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The map label for this gene is ilvI [H]

Identifier: 73540650

GI number: 73540650

Start: 1033639

End: 1035402

Strand: Direct

Name: ilvI [H]

Synonym: Reut_A0947

Alternate gene names: 73540650

Gene position: 1033639-1035402 (Clockwise)

Preceding gene: 73540639

Following gene: 73540651

Centisome position: 27.15

GC content: 61.85

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCCACGCAGACAGCAATTCATCTGCCGCACCCGAAATGATCGGTGCGGAAATCCT
CGTTCATGCACTTGCCGAAGAAGGCGTTGAATACGTCTGGGGTTATCCCGGCGGCGCAGTGCTGTATATCTACGACGAGC
TTCACAAGCAAAAGAAATTCGAGCACATCCTGGTGCGCCACGAGCAGGCCGCGGTCCATGCCGCAGACGGCTATGCACGG
GCAACCGGCAAGGTGGGCGTCGCCCTGGTGACCTCGGGTCCCGGCGTGACCAATGCCGTCACGGGCATTGCCACCGCGTA
CCTCGATTCGATCCCGATGGTGGTGATCACCGGCAACGTGCCGACCCACGCGATCGGCCAGGATGCCTTCCAGGAATGCG
ACACCGTCGGGATTACGCGCCCGATCGTCAAGCACAACTTCCTGGTGAAGGACGTGCGCGATCTCGCCTCGACCATCAAG
AAGGCCTTCTACATTGCTTCGACCGGCCGTCCGGGCCCGGTGGTCGTGGACATCCCCAAGGATGTTTCGCGCAACACCTG
CAAGTACGAGTACCCGAAGTCGCTTGACATGCGCTCGTACAACCCGGTCAACAAGGGCCACTCGGGCCAGATCCGCAAGG
CCGTGGCGCTGCTGCAGGGCGCCGAGCGTCCGTACATCTACGCCGGCGGCGGCGTGGTGCTGGCCAACGCCAGCGAGGAA
CTGCGCCAGCTCGCGGCCCTCACGGGCCACCCGGTCACAAACACCCTGATGGGCCTGGGCTCGTTCCCCGGCACCAGCAA
GCAGTTCGTCGGCATGCTTGGCATGCACGGCACGTATGAAGCCAACATGGCCATGCAGAACTGCGACGTGCTGATTGCCA
TCGGTGCGCGTTTCGATGACCGCGTGATCGGCAACCCGTCGCACTTCACCTCGCAGGCGCGCAAGATCGTCCATATCGAT
ATCGACCCGTCGTCGATCTCGAAGCGCGTCAAGGTGGATATCCCCATCGTCGGCAACGTCAAGGACGTGCTGCAGGAACT
GATCGCGCAGCTGCAGGCCAGCGACGTCAAGCCCAAGCGCGAGGCGCTCGCCAAGTGGTGGGAGCAGATCGAGCAATGGC
GTTCGGTGGACTGCCTCAAGTACGACCGCAGCTCCGAGATCATCAAGCCGCAATACGTAGTCGAGAAGATCTGGGAACTG
ACCAAGGGCGATGCGTTCATCTGCTCCGACGTCGGCCAGCACCAGATGTGGGCCGCGCAGTTCTACAAGTTCAACGAGCC
GCGCCGCTGGATCAACTCGGGCGGCTTGGGCACGATGGGCGTAGGCCTGCCGTACGCAATGGGCATCAAGAAGGCGTTTC
CGGAGAAGGAAGTCGTCACCATCACCGGTGAGGGCTCGATCCAGATGTGTATCCAGGAACTGTCGACCTGCCTGCAGTTC
GACACCCCCGTCAAGATCTGCTCGCTCAACAACGGCTACCTCGGCATGGTGCGCCAGTGGCAGGAAATCGAGTACGACAA
CCGCTACTCGCATTCCTACATGAATGCGCTGCCCGACTTCGTGAAACTGGCTGAGGCCTATGGCCACGTCGGCATGCGTG
TGGAAAAGACGTCCGACGTCGAGCCGGCGCTGCGCGAGGCCTTCCGCCTGAAGGACCGTACCGTGTTCCTGGACTTCCAG
ACCGATCCCACTGAAAACGTCTGGCCGATGGTCCAGGCGGGCAAGGGCATTTCGGAAATGCTGCTCGGCGCGGAGGACCT
GTAA

Upstream 100 bases:

>100_bases
AGGCGGTCTTCTCATGCAGACGCGCACCGCTTGAACCCGTGCCACAAGCCCGGCAGAGAGCATCCAAACAATTTTTTGCC
GAAAATTGCAAAGGACTGAA

Downstream 100 bases:

>100_bases
ATGCGACACATCATTTCGGTCCTGCTGGAAAACGAACCGGGCGCGCTGTCGCGCGTGGTGGGCCTGTTCTCGGCCCGCGG
CTATAACATCGAGACGCTGA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSHADSNSSAAPEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQKKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLASTIK
KAFYIASTGRPGPVVVDIPKDVSRNTCKYEYPKSLDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYAGGGVVLANASEE
LRQLAALTGHPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTSQARKIVHID
IDPSSISKRVKVDIPIVGNVKDVLQELIAQLQASDVKPKREALAKWWEQIEQWRSVDCLKYDRSSEIIKPQYVVEKIWEL
TKGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQF
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMNALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL

Sequences:

>Translated_587_residues
MNMPSAEFSHADSNSSAAPEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQKKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLASTIK
KAFYIASTGRPGPVVVDIPKDVSRNTCKYEYPKSLDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYAGGGVVLANASEE
LRQLAALTGHPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTSQARKIVHID
IDPSSISKRVKVDIPIVGNVKDVLQELIAQLQASDVKPKREALAKWWEQIEQWRSVDCLKYDRSSEIIKPQYVVEKIWEL
TKGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQF
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMNALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL
>Mature_587_residues
MNMPSAEFSHADSNSSAAPEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQKKFEHILVRHEQAAVHAADGYAR
ATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLASTIK
KAFYIASTGRPGPVVVDIPKDVSRNTCKYEYPKSLDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYAGGGVVLANASEE
LRQLAALTGHPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPSHFTSQARKIVHID
IDPSSISKRVKVDIPIVGNVKDVLQELIAQLQASDVKPKREALAKWWEQIEQWRSVDCLKYDRSSEIIKPQYVVEKIWEL
TKGDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQF
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMNALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFLDFQ
TDPTENVWPMVQAGKGISEMLLGAEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=577, Percent_Identity=24.9566724436742, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=25.78125, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=56.8661971830986, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=574, Percent_Identity=39.7212543554007, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=34.5656192236599, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=29.4014084507042, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=24.5045045045045, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=546, Percent_Identity=26.9230769230769, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=546, Percent_Identity=26.9230769230769, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=511, Percent_Identity=26.027397260274, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=43.4931506849315, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=554, Percent_Identity=24.0072202166065, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320123, Length=513, Percent_Identity=22.0272904483431, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=520, Percent_Identity=22.6923076923077, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=25.9130434782609, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64735; Mature: 64735

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSHADSNSSAAPEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQKKF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHH
EHILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNV
HHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLASTIKKAFYIASTGRPGPVVVDIPK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSRNTCKYEYPKSLDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYAGGGVVLANASEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LRQLAALTGHPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDD
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCC
RVIGNPSHFTSQARKIVHIDIDPSSISKRVKVDIPIVGNVKDVLQELIAQLQASDVKPKR
CCCCCCHHHHHHCCEEEEEECCHHHHCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCHHH
EALAKWWEQIEQWRSVDCLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQ
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
FYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQF
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMNALPDFVKLAEAYGHVGMRVEKTSDV
CCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSHADSNSSAAPEMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQKKF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEHHHHHHHHH
EHILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNV
HHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRDLASTIKKAFYIASTGRPGPVVVDIPK
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DVSRNTCKYEYPKSLDMRSYNPVNKGHSGQIRKAVALLQGAERPYIYAGGGVVLANASEE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LRQLAALTGHPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDD
HHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCC
RVIGNPSHFTSQARKIVHIDIDPSSISKRVKVDIPIVGNVKDVLQELIAQLQASDVKPKR
CCCCCCHHHHHHCCEEEEEECCHHHHCCEEEEEEEEECCHHHHHHHHHHHHHHCCCCHHH
EALAKWWEQIEQWRSVDCLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQ
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
FYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQF
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMNALPDFVKLAEAYGHVGMRVEKTSDV
CCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]