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The map label for this gene is oprM [H]

Identifier: 73540628

GI number: 73540628

Start: 1008260

End: 1009744

Strand: Direct

Name: oprM [H]

Synonym: Reut_A0925

Alternate gene names: 73540628

Gene position: 1008260-1009744 (Clockwise)

Preceding gene: 73540627

Following gene: 73540629

Centisome position: 26.49

GC content: 64.71

Gene sequence:

>1485_bases
ATGCAGTTGCAGGAAGACATCGTGCCCAAGGTCTCTACCGAATCGTCATGGATTGTTCCACGGCCGCGCCGCGCTTATGC
CTGGGTCGCCGCTGCGGCCTGCGCAGGATGGCTCACTGGCTGTGCCGATTTCCGTCCGCCGGTGTACGAGCGTCCGGAAA
CGCCGGCCAAGACGGAATGGTCGCGCCAGGAGTCGATTACGGTATCGCCGGCCGAGACCGTGCAGCCGAACTGGTGGCAA
GGATTCAAGGACCCGTACCTGGACAAGCTGATCACACAGGCGCTGTCCGGCAACTACGACATCAAGGTGCTCGCCGCACG
CATCCGCGTGGCGCAGGCGCAGATCGGTGAAGCGAAGGCCGGTCAGTTGCCGGTGATCGACCTCAACGCCAACGCGAACG
TCACCAAGACCACCGGCCAGGCCACCGCGCGCAGCTACAACCTGGGTGGCCAGCTGAGCTGGGACATCGACATCTGGGGC
AAGGTAGAGAAGGGCGTGCAGGCGCAGACCGCCGAGTTCCACGCCACCGAAGCGGACTGGCGCGCCGGCTACCTGACGCT
GGCGGCCAATGTCTCGACCGCTTACTTCCAGATCCTGCAGTTCGACGAGCAGATCGACCAGCAGACGCGCACCGTGCAGA
AGAACGGGCAAATCCTCAATACATACCAGGCCATGTTCCAGAACGGGCTGGTGCCGAAGATCCGCGTCATGCAGCAGCAG
GCGGAAATCAACCGGCTGACCAAGGACCTGATCGAACTGCGCCGCTCGCGTGACGTGACGGAGAACGCGCTGGCCACGCT
GGTCGGCGTGCCCGCCGGCAATCTCAAGGTGCCGCCCGGCAAGCTGCAGGACCGCGTGCAGCCGCCGCCGGTGCCGGCGG
GCCTGCCGTCGCAGTTGCTGGCGCGCCGGCCCGACATCGTGGCCGCGGAGTATCGCGTGCTCGCGGCGTACAACATCGTC
GGCCAGGCCCGGCTTGCGCAGCTGCCGAGCATCAGCCTGACCGCGAACGGCGGCATGGCCAGCTTCGCGCTGAACAGCTT
GCTGAAGACTTTCACGCTCGGCATTGCGCCCAGCATCACTCTGCCGATGCTGGACCCGAACATTCGCGCTCGCGTCAAGA
CCACGGAGGCGCAGGTCAAGGTGGTGGAAAGCGACTACGGCCGCACCGTGATGAACGCGTTCGAAGAGGTGGAGACCGCA
CTGGTCAATGTCGACGCGCACAAGAAGCAGCGCATCGAGTTGCAGCAGCAGGTTGACCAGCTGCGCGTGGTGTCGTCGCA
GATCGAGGCGCAGCTCAAGGAAGGCGTTGTGTCGCAGCTTGAAGTGTTCGAGACCGAGCGTTCGCTGCTGAGCGCGCAAC
TGTCCTTGTTGGCCGTCCACCAACAGATCCTTGCCGATACTGTCACGCTCTACAAGGCGCTAGGCGGCGGCTGGACCGAC
GTGCGGGTCAGCAACGCCACGGCAAGCACTTCGTCGTCGCAGTGA

Upstream 100 bases:

>100_bases
CCGACCTCACCGCCAACCTCATTTCGGGGTTGGAGCGCGCGCAAGCGCGAACCACAATACAGACACGCAGGGCGGCGCCA
CTTCGGTGGCGGCGTCATGC

Downstream 100 bases:

>100_bases
CCTGGCGCCTTTGCGCCAGGCAGCGTTCACGATACATCGGCTCCCCGGCGCGCAGGGTACGCCAGCGGTTTGCGCCGCAT
GGGCGACACTCTGCGCGCTA

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MQLQEDIVPKVSTESSWIVPRPRRAYAWVAAAACAGWLTGCADFRPPVYERPETPAKTEWSRQESITVSPAETVQPNWWQ
GFKDPYLDKLITQALSGNYDIKVLAARIRVAQAQIGEAKAGQLPVIDLNANANVTKTTGQATARSYNLGGQLSWDIDIWG
KVEKGVQAQTAEFHATEADWRAGYLTLAANVSTAYFQILQFDEQIDQQTRTVQKNGQILNTYQAMFQNGLVPKIRVMQQQ
AEINRLTKDLIELRRSRDVTENALATLVGVPAGNLKVPPGKLQDRVQPPPVPAGLPSQLLARRPDIVAAEYRVLAAYNIV
GQARLAQLPSISLTANGGMASFALNSLLKTFTLGIAPSITLPMLDPNIRARVKTTEAQVKVVESDYGRTVMNAFEEVETA
LVNVDAHKKQRIELQQQVDQLRVVSSQIEAQLKEGVVSQLEVFETERSLLSAQLSLLAVHQQILADTVTLYKALGGGWTD
VRVSNATASTSSSQ

Sequences:

>Translated_494_residues
MQLQEDIVPKVSTESSWIVPRPRRAYAWVAAAACAGWLTGCADFRPPVYERPETPAKTEWSRQESITVSPAETVQPNWWQ
GFKDPYLDKLITQALSGNYDIKVLAARIRVAQAQIGEAKAGQLPVIDLNANANVTKTTGQATARSYNLGGQLSWDIDIWG
KVEKGVQAQTAEFHATEADWRAGYLTLAANVSTAYFQILQFDEQIDQQTRTVQKNGQILNTYQAMFQNGLVPKIRVMQQQ
AEINRLTKDLIELRRSRDVTENALATLVGVPAGNLKVPPGKLQDRVQPPPVPAGLPSQLLARRPDIVAAEYRVLAAYNIV
GQARLAQLPSISLTANGGMASFALNSLLKTFTLGIAPSITLPMLDPNIRARVKTTEAQVKVVESDYGRTVMNAFEEVETA
LVNVDAHKKQRIELQQQVDQLRVVSSQIEAQLKEGVVSQLEVFETERSLLSAQLSLLAVHQQILADTVTLYKALGGGWTD
VRVSNATASTSSSQ
>Mature_494_residues
MQLQEDIVPKVSTESSWIVPRPRRAYAWVAAAACAGWLTGCADFRPPVYERPETPAKTEWSRQESITVSPAETVQPNWWQ
GFKDPYLDKLITQALSGNYDIKVLAARIRVAQAQIGEAKAGQLPVIDLNANANVTKTTGQATARSYNLGGQLSWDIDIWG
KVEKGVQAQTAEFHATEADWRAGYLTLAANVSTAYFQILQFDEQIDQQTRTVQKNGQILNTYQAMFQNGLVPKIRVMQQQ
AEINRLTKDLIELRRSRDVTENALATLVGVPAGNLKVPPGKLQDRVQPPPVPAGLPSQLLARRPDIVAAEYRVLAAYNIV
GQARLAQLPSISLTANGGMASFALNSLLKTFTLGIAPSITLPMLDPNIRARVKTTEAQVKVVESDYGRTVMNAFEEVETA
LVNVDAHKKQRIELQQQVDQLRVVSSQIEAQLKEGVVSQLEVFETERSLLSAQLSLLAVHQQILADTVTLYKALGGGWTD
VRVSNATASTSSSQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=454, Percent_Identity=29.5154185022026, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI2367348, Length=463, Percent_Identity=26.133909287257, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54252; Mature: 54252

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQEDIVPKVSTESSWIVPRPRRAYAWVAAAACAGWLTGCADFRPPVYERPETPAKTEW
CCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SRQESITVSPAETVQPNWWQGFKDPYLDKLITQALSGNYDIKVLAARIRVAQAQIGEAKA
CCCCCEEECCHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCC
GQLPVIDLNANANVTKTTGQATARSYNLGGQLSWDIDIWGKVEKGVQAQTAEFHATEADW
CCCCEEEECCCCCEEECCCCHHHHEECCCCEEEEEEEECCHHHCCCCHHHHHHCCCCCCC
RAGYLTLAANVSTAYFQILQFDEQIDQQTRTVQKNGQILNTYQAMFQNGLVPKIRVMQQQ
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
AEINRLTKDLIELRRSRDVTENALATLVGVPAGNLKVPPGKLQDRVQPPPVPAGLPSQLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHH
ARRPDIVAAEYRVLAAYNIVGQARLAQLPSISLTANGGMASFALNSLLKTFTLGIAPSIT
HCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
LPMLDPNIRARVKTTEAQVKVVESDYGRTVMNAFEEVETALVNVDAHKKQRIELQQQVDQ
EECCCCCCEEEEEECHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRVVSSQIEAQLKEGVVSQLEVFETERSLLSAQLSLLAVHQQILADTVTLYKALGGGWTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VRVSNATASTSSSQ
EEEECCCCCCCCCC
>Mature Secondary Structure
MQLQEDIVPKVSTESSWIVPRPRRAYAWVAAAACAGWLTGCADFRPPVYERPETPAKTEW
CCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SRQESITVSPAETVQPNWWQGFKDPYLDKLITQALSGNYDIKVLAARIRVAQAQIGEAKA
CCCCCEEECCHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCC
GQLPVIDLNANANVTKTTGQATARSYNLGGQLSWDIDIWGKVEKGVQAQTAEFHATEADW
CCCCEEEECCCCCEEECCCCHHHHEECCCCEEEEEEEECCHHHCCCCHHHHHHCCCCCCC
RAGYLTLAANVSTAYFQILQFDEQIDQQTRTVQKNGQILNTYQAMFQNGLVPKIRVMQQQ
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHH
AEINRLTKDLIELRRSRDVTENALATLVGVPAGNLKVPPGKLQDRVQPPPVPAGLPSQLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHH
ARRPDIVAAEYRVLAAYNIVGQARLAQLPSISLTANGGMASFALNSLLKTFTLGIAPSIT
HCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
LPMLDPNIRARVKTTEAQVKVVESDYGRTVMNAFEEVETALVNVDAHKKQRIELQQQVDQ
EECCCCCCEEEEEECHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LRVVSSQIEAQLKEGVVSQLEVFETERSLLSAQLSLLAVHQQILADTVTLYKALGGGWTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VRVSNATASTSSSQ
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]