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The map label for this gene is citH [H]

Identifier: 73540600

GI number: 73540600

Start: 979143

End: 980441

Strand: Direct

Name: citH [H]

Synonym: Reut_A0897

Alternate gene names: 73540600

Gene position: 979143-980441 (Clockwise)

Preceding gene: 73540599

Following gene: 73540602

Centisome position: 25.72

GC content: 65.59

Gene sequence:

>1299_bases
ATGTCCATCGACACCACCGCCGGCGCGGCGATCGCGGCGCCGCAGCCGCAGCTGTCCCGCGCGCGCGCCATTGCCGCCAT
CACCATCGGCAACGGCCTGGAGTTCTACGATTTCGTCGTCTACAGCTTCTTTGCCACGCTGATCGGTCGGCTGTATTTCC
CGGTCGACAACCCGACCGGCCAGCTGCTGATGTCGTTCGCCACGTTCGGCGTCGGCTTCCTGATGCGTCCGCTCGGCGGC
TTGCTGATCGGCATGTACGCCGACCGCGCCGGGCGCAAGCCGGCCGTGGCGCTAACACTGTGGCTGATGGGCCTGAGCTC
GCTGATTTTCGTGGTGACGCCGTCGTATGCGCAAATCGGCATCTTCGCGCCGGCCATGGTGGTGATCGCGCGGCTGGTGC
AGGGCTTTGCCATTGGCGGCGAGATGGGCGCATCGACCGCGCTGCTGCTTGAGTACGCCGATGACCGTACGCGCGGCTTC
TATACCGCGTGGCAGCCGTTCAGCCAGGGCCTGGCGGCGCTGATGGGGGCGATCACGGGCCTGGTGCTCAGCAATGTGCT
GGCCCCGGCCGAGCTGGAATCGTGGGGCTGGCGCCTTGCGTTCCTGATCGGCATTCTGGTCATTCCGGTAGGCCTGTTGA
TCCGTCGTCGGCTGGAAGAAACTGCAGAGCCTTTGCACCGAGGCGAACACGCTGCGCAATGGCCGCTGCTGCGCCAGCAT
GCACGCGAAGTGCTCGCAAGCGTCCTGCTGATGATCGGTCTGGCCTCGTCGACCTATATCGTTATCTACTACCTCAGCAA
CTACGCCGTGAGCGTGCTGAAGATGCCGCTGTCGCTGGGCATCTGGGCAGGCTGCATCGCGTCGGCGGTGCAGGTGGTGC
TGTCGCCGTTCGCCGGCTGGCTGGCCGACCGCGTCGGCCGCAAGACAGTGGTGCTGTGGTCGCGCGTCGCGCTGCTGGTG
ATGGTGTACCCGGCCTTCGTGCTGATCAACGCCGAGCCTTCGCTGGCGCGTCTGCTGGTCGTGGTGGCCTGCCTGTCGGT
GCCGATGTCGATGACCTCGCCCGCGTCGATGGTGCTGGTCAGCGAGGTGCTGCCGAAGCGTCTGCGCGCCACCGGCTTGT
CGATCGCGTATTGCGTGGCGATTGCCATCTTCGGCGGCTTTGCGCAGTATTTCTCGACGCGTCTGATCCAGATCACTGGT
GATGCCAATGCACCGGCGCTCTACGTGATCGGTTGCGGTCTGGTGTCGCTGGTCGGTCTGGCGCTGGTGCCCGAGACGCT
GGGGCGTCGCCTGAAATAA

Upstream 100 bases:

>100_bases
GCGCAAGTAAAGCGCGACCGAGCCGGCCGCAAGAGCCGTACTGATGACCGGGTTACCTCGCGTGCGCCCGGTCGCTGCTA
TCCCATTCACGGAGACACCC

Downstream 100 bases:

>100_bases
TCGCTGCGGTAGCGTTTGCTGGCAGGCACGCCTATGCATTAGCGCGGGACCAGCCGCTCAGCGCGCCTGCAAGTTACCCC
AGCGGTCCTGGCCTCCTGCC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSIDTTAGAAIAAPQPQLSRARAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVDNPTGQLLMSFATFGVGFLMRPLGG
LLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPSYAQIGIFAPAMVVIARLVQGFAIGGEMGASTALLLEYADDRTRGF
YTAWQPFSQGLAALMGAITGLVLSNVLAPAELESWGWRLAFLIGILVIPVGLLIRRRLEETAEPLHRGEHAAQWPLLRQH
AREVLASVLLMIGLASSTYIVIYYLSNYAVSVLKMPLSLGIWAGCIASAVQVVLSPFAGWLADRVGRKTVVLWSRVALLV
MVYPAFVLINAEPSLARLLVVVACLSVPMSMTSPASMVLVSEVLPKRLRATGLSIAYCVAIAIFGGFAQYFSTRLIQITG
DANAPALYVIGCGLVSLVGLALVPETLGRRLK

Sequences:

>Translated_432_residues
MSIDTTAGAAIAAPQPQLSRARAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVDNPTGQLLMSFATFGVGFLMRPLGG
LLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPSYAQIGIFAPAMVVIARLVQGFAIGGEMGASTALLLEYADDRTRGF
YTAWQPFSQGLAALMGAITGLVLSNVLAPAELESWGWRLAFLIGILVIPVGLLIRRRLEETAEPLHRGEHAAQWPLLRQH
AREVLASVLLMIGLASSTYIVIYYLSNYAVSVLKMPLSLGIWAGCIASAVQVVLSPFAGWLADRVGRKTVVLWSRVALLV
MVYPAFVLINAEPSLARLLVVVACLSVPMSMTSPASMVLVSEVLPKRLRATGLSIAYCVAIAIFGGFAQYFSTRLIQITG
DANAPALYVIGCGLVSLVGLALVPETLGRRLK
>Mature_431_residues
SIDTTAGAAIAAPQPQLSRARAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVDNPTGQLLMSFATFGVGFLMRPLGGL
LIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPSYAQIGIFAPAMVVIARLVQGFAIGGEMGASTALLLEYADDRTRGFY
TAWQPFSQGLAALMGAITGLVLSNVLAPAELESWGWRLAFLIGILVIPVGLLIRRRLEETAEPLHRGEHAAQWPLLRQHA
REVLASVLLMIGLASSTYIVIYYLSNYAVSVLKMPLSLGIWAGCIASAVQVVLSPFAGWLADRVGRKTVVLWSRVALLVM
VYPAFVLINAEPSLARLLVVVACLSVPMSMTSPASMVLVSEVLPKRLRATGLSIAYCVAIAIFGGFAQYFSTRLIQITGD
ANAPALYVIGCGLVSLVGLALVPETLGRRLK

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=431, Percent_Identity=32.7146171693735, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=30.3317535545024, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788942, Length=420, Percent_Identity=32.6190476190476, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=30.1624129930394, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=29.646017699115, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46272; Mature: 46140

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDTTAGAAIAAPQPQLSRARAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVDNPTG
CCCCCCCCCEECCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHH
QLLMSFATFGVGFLMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPSYAQIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCHHHHH
IFAPAMVVIARLVQGFAIGGEMGASTALLLEYADDRTRGFYTAWQPFSQGLAALMGAITG
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSNVLAPAELESWGWRLAFLIGILVIPVGLLIRRRLEETAEPLHRGEHAAQWPLLRQH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AREVLASVLLMIGLASSTYIVIYYLSNYAVSVLKMPLSLGIWAGCIASAVQVVLSPFAGW
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADRVGRKTVVLWSRVALLVMVYPAFVLINAEPSLARLLVVVACLSVPMSMTSPASMVLV
HHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
SEVLPKRLRATGLSIAYCVAIAIFGGFAQYFSTRLIQITGDANAPALYVIGCGLVSLVGL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHHHHH
ALVPETLGRRLK
HHHHHHHCCCCC
>Mature Secondary Structure 
SIDTTAGAAIAAPQPQLSRARAIAAITIGNGLEFYDFVVYSFFATLIGRLYFPVDNPTG
CCCCCCCCEECCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHH
QLLMSFATFGVGFLMRPLGGLLIGMYADRAGRKPAVALTLWLMGLSSLIFVVTPSYAQIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCHHHHH
IFAPAMVVIARLVQGFAIGGEMGASTALLLEYADDRTRGFYTAWQPFSQGLAALMGAITG
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSNVLAPAELESWGWRLAFLIGILVIPVGLLIRRRLEETAEPLHRGEHAAQWPLLRQH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AREVLASVLLMIGLASSTYIVIYYLSNYAVSVLKMPLSLGIWAGCIASAVQVVLSPFAGW
HHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADRVGRKTVVLWSRVALLVMVYPAFVLINAEPSLARLLVVVACLSVPMSMTSPASMVLV
HHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHH
SEVLPKRLRATGLSIAYCVAIAIFGGFAQYFSTRLIQITGDANAPALYVIGCGLVSLVGL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHHHHH
ALVPETLGRRLK
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]