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The map label for this gene is gcvA [H]

Identifier: 73540594

GI number: 73540594

Start: 972888

End: 973787

Strand: Direct

Name: gcvA [H]

Synonym: Reut_A0891

Alternate gene names: 73540594

Gene position: 972888-973787 (Clockwise)

Preceding gene: 73540593

Following gene: 73540597

Centisome position: 25.56

GC content: 66.0

Gene sequence:

>900_bases
ATGCGCATCTCCCCGCTACCGCCCCTGCCTAACCTTGTCGCCTTCGAAGCCGCGATGCGGCATGGCAGCTTCACCCGTGC
CGCAGCGGAGCTTCACCTGACGCAAAGCGCGATCAGCCGCCAGGTCGCCCAGCTCGAGAGCTTTCTCGGACGCAAGCTGT
TCATCCGCGAGCCGCGCGCGCTGCGGCTGACGGTCAGCGGCCAGCGCTATGCAGAGGTTGTGCAGCGTTTGCTGGTCGGT
TGCGCCGAAGCGACCGAAGACGTGATGAAAGTGCGCAGCCACACCGCCCTGACTGTGGCGTGCTCCACGGGGGTGGCCGT
GCTGTGGCTGACACCGCGTCTGGCATCGTTCCGCGCAGCGCACCCGGAGATCCAGTTGCGGCTGATCGTCAATGACAGCC
TTGCGTCGCTGTCCGCCGCGGAATTCGATATTGGCCTGTACTACTTGCGCGAAGGTCCACCGGCGGGCTTTGCGGCACGG
CGCCTATATGGCGAGGAAGTCTTTCCCGTGTGCGCGCCAGCCTATCTCGGCGGGCGCGCTCTGGCCCCGTCGGCGCTGGT
CAACGAGACCTTGCTGATGCTCGACGACGGCCAGCGGCAATGGATGTCATGGCAAGCATGGTTTGCCCACCAGGGCGTGC
ATGACGCGAATCCGCGCGCGTTTCTGACATCGAACCAGTATCCGATCCTGCTGCAACTGGCGATAGAAGGTCAGGGCATC
GCGCTCGGCTGGCGGCATATGATCGACGGATGCCTGCGCGACGGCCTGCTGGTGCGTGCCTGCGAAGCGAGCGCGAGCCT
GGGCGGGGGCTACTACGTGGTTTGGCCGCGCGATCGCATGGAGCCGGCAGCAGCGCGCGCATTCCGCAACTGGATGGTGC
GGCAGGCATCGACGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGGACGTGCCGGAGCCGGGCTCATTCGCTCACGCTTTCCTCTCCCTTTGCGTCGTATTCGCGGTCTATCATGGCGAC
TTCAGCGACCGGAAGCTTCC

Downstream 100 bases:

>100_bases
CACGCAGCCCCGCTCGGGGCTGCCGTGTGCTTCAGTACCCCGCCGCCCGATCGACCAGGTTCAACAACTCCCTGCCATCC
CGCATCCGCCGCACGTTCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLESFLGRKLFIREPRALRLTVSGQRYAEVVQRLLVG
CAEATEDVMKVRSHTALTVACSTGVAVLWLTPRLASFRAAHPEIQLRLIVNDSLASLSAAEFDIGLYYLREGPPAGFAAR
RLYGEEVFPVCAPAYLGGRALAPSALVNETLLMLDDGQRQWMSWQAWFAHQGVHDANPRAFLTSNQYPILLQLAIEGQGI
ALGWRHMIDGCLRDGLLVRACEASASLGGGYYVVWPRDRMEPAAARAFRNWMVRQASTA

Sequences:

>Translated_299_residues
MRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLESFLGRKLFIREPRALRLTVSGQRYAEVVQRLLVG
CAEATEDVMKVRSHTALTVACSTGVAVLWLTPRLASFRAAHPEIQLRLIVNDSLASLSAAEFDIGLYYLREGPPAGFAAR
RLYGEEVFPVCAPAYLGGRALAPSALVNETLLMLDDGQRQWMSWQAWFAHQGVHDANPRAFLTSNQYPILLQLAIEGQGI
ALGWRHMIDGCLRDGLLVRACEASASLGGGYYVVWPRDRMEPAAARAFRNWMVRQASTA
>Mature_299_residues
MRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLESFLGRKLFIREPRALRLTVSGQRYAEVVQRLLVG
CAEATEDVMKVRSHTALTVACSTGVAVLWLTPRLASFRAAHPEIQLRLIVNDSLASLSAAEFDIGLYYLREGPPAGFAAR
RLYGEEVFPVCAPAYLGGRALAPSALVNETLLMLDDGQRQWMSWQAWFAHQGVHDANPRAFLTSNQYPILLQLAIEGQGI
ALGWRHMIDGCLRDGLLVRACEASASLGGGYYVVWPRDRMEPAAARAFRNWMVRQASTA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=36.0544217687075, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=31.4569536423841, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=27.5167785234899, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=28.6195286195286, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790262, Length=267, Percent_Identity=27.3408239700375, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=24.5674740484429, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=35, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789440, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081978, Length=245, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=27.0491803278689, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789204, Length=207, Percent_Identity=31.4009661835749, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=236, Percent_Identity=26.271186440678, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790208, Length=242, Percent_Identity=26.4462809917355, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLESFLGRKLFIREPRA
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCE
LRLTVSGQRYAEVVQRLLVGCAEATEDVMKVRSHTALTVACSTGVAVLWLTPRLASFRAA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHCC
HPEIQLRLIVNDSLASLSAAEFDIGLYYLREGPPAGFAARRLYGEEVFPVCAPAYLGGRA
CCCEEEEEEECCCCHHCCHHEEEEEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHCCCC
LAPSALVNETLLMLDDGQRQWMSWQAWFAHQGVHDANPRAFLTSNQYPILLQLAIEGQGI
CCCHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCE
ALGWRHMIDGCLRDGLLVRACEASASLGGGYYVVWPRDRMEPAAARAFRNWMVRQASTA
EEHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLESFLGRKLFIREPRA
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCE
LRLTVSGQRYAEVVQRLLVGCAEATEDVMKVRSHTALTVACSTGVAVLWLTPRLASFRAA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHCC
HPEIQLRLIVNDSLASLSAAEFDIGLYYLREGPPAGFAARRLYGEEVFPVCAPAYLGGRA
CCCEEEEEEECCCCHHCCHHEEEEEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHCCCC
LAPSALVNETLLMLDDGQRQWMSWQAWFAHQGVHDANPRAFLTSNQYPILLQLAIEGQGI
CCCHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCCCE
ALGWRHMIDGCLRDGLLVRACEASASLGGGYYVVWPRDRMEPAAARAFRNWMVRQASTA
EEHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]