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The map label for this gene is citA [H]

Identifier: 73540592

GI number: 73540592

Start: 970188

End: 971531

Strand: Direct

Name: citA [H]

Synonym: Reut_A0889

Alternate gene names: 73540592

Gene position: 970188-971531 (Clockwise)

Preceding gene: 73540591

Following gene: 73540593

Centisome position: 25.49

GC content: 64.06

Gene sequence:

>1344_bases
ATGCACAACAGCGCCACGCCAACGAGCGCCACCGCCACCGCCACCTATTCATCGACGGCTCCGGCCCGGTCCGCAGCTGT
CATCGAGCCCGCCCAACGGCGCCGCGCCATCATCGCCACGGTGATCGGCAATGGGCTGGAGTGGTTCGACTTTACCGTCT
ACAGCTTCTTCGCAATCATCATCGCGAAGCTGTTCTTTCCGACCGGCAATGACCTTAGCTCGCTACTACTCGCCGTGGCG
ACTTTCGGTGTGGGCTTCTTCATGCGTCCTGTCGGCGGCATCGTGCTCGGCGTTTATGCTGACCGTGTCGGCCGCAAGGC
CGCGCTGTCGATGACGATTCTGCTGATGGCGCTGGGCACCACGCTGATCGGCATCGCGCCGACCTACGAGCAGATGGGGA
TCTTCGCTCCGCTCTTGATCGTGGTGGCGCGCCTGCTGCAAGGTTTCTCCGCCGGCGGCGAGATGGGCGGCGCCACGGCC
TTCCTGACCGAGTATGCGCCGGAACGCGAGCGCGCCTACTACTCGAGCTGGATCCAGGCAAGCATCGGCGTGGCCGTGCT
GCTTGGCGCGGCAGTCGGCACCTTCGTCACGCGTTCGCTGAGTCCTGAAGCGCTCAACAGCTGGGGCTGGCGCCTGCCGT
TCCTGCTTGGCATCGTCATCGGGCCGGTGGGCTACTACATCCGCCATCATCTCGACGAGACCCCGACCTTCCGCGAAACA
GACGAACGCGCCGACTCGCCGCTGAAGGAGGTGTTCCAGGCTTACCCGCGCGAAACGCTGGCCAGCTTTTCGATGGTGAT
CCTTTGGACGGTGTGCACCTACGTCTTGCTGTTCTACATGCCGACCTATGCCGTCAAGGTGCTGAACGTGCCGCAGTCGG
ACGGCTTTGTCGCGGGCATGGTCGGTGGCTGCACGATCATGCTGTTCGCACCGCTGGTCGGCCGGCTCGCGGACCGCATC
GGCCGGCGCGCACTGCTGAGCGGATCGGCGCTGCTGATCCTGGTGCTGGCCTGGCCGATGTTCGCGTACATCAATCATGT
GCCGGGGCTGTCGTCGCTGCTGGTTTTCCAGCTCGTGTTCGGCATCCTGATCGCTGCCTATACCGGCCCGATTCTGGCCG
CATTCTCGGAGCTGTTCCCGGCGCGCGTGCTGTCAACCGGCCTGTCGGTGGCTTACAACTTCGCGGTGACGATCTTCGGT
GGTTTTGCGTCGTTCATCATCACGTGGCTGATTGCTACCACGGGCAGCAGCATGGCGCCCGCAATCTACGTCATGATCGC
GGCCGCCATCAGCCTGGCCGGCACGCGCCTTGTCCGTGAACCCGCTTACCTGCAACAACGCTGA

Upstream 100 bases:

>100_bases
TCGCTGCCTCCCTTCTCCTAACATCGGCTCACCATCTGCCAGTCAGCCGTGCGGTCCATGCGCTACGAGCCGCGGCGGTG
GGCTACCGACGAGGAGACCC

Downstream 100 bases:

>100_bases
TATGACCCAGATTCCACAGATCCTCTTTCACGGCGGCGACGTGCTCGACCCCGGCATGGGCGAGTTGCTGCGCGGCCATG
ATGTCCTCGTCGAGGGCGAG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MHNSATPTSATATATYSSTAPARSAAVIEPAQRRRAIIATVIGNGLEWFDFTVYSFFAIIIAKLFFPTGNDLSSLLLAVA
TFGVGFFMRPVGGIVLGVYADRVGRKAALSMTILLMALGTTLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATA
FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRET
DERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLNVPQSDGFVAGMVGGCTIMLFAPLVGRLADRI
GRRALLSGSALLILVLAWPMFAYINHVPGLSSLLVFQLVFGILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG
GFASFIITWLIATTGSSMAPAIYVMIAAAISLAGTRLVREPAYLQQR

Sequences:

>Translated_447_residues
MHNSATPTSATATATYSSTAPARSAAVIEPAQRRRAIIATVIGNGLEWFDFTVYSFFAIIIAKLFFPTGNDLSSLLLAVA
TFGVGFFMRPVGGIVLGVYADRVGRKAALSMTILLMALGTTLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATA
FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRET
DERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLNVPQSDGFVAGMVGGCTIMLFAPLVGRLADRI
GRRALLSGSALLILVLAWPMFAYINHVPGLSSLLVFQLVFGILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG
GFASFIITWLIATTGSSMAPAIYVMIAAAISLAGTRLVREPAYLQQR
>Mature_447_residues
MHNSATPTSATATATYSSTAPARSAAVIEPAQRRRAIIATVIGNGLEWFDFTVYSFFAIIIAKLFFPTGNDLSSLLLAVA
TFGVGFFMRPVGGIVLGVYADRVGRKAALSMTILLMALGTTLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATA
FLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRET
DERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLNVPQSDGFVAGMVGGCTIMLFAPLVGRLADRI
GRRALLSGSALLILVLAWPMFAYINHVPGLSSLLVFQLVFGILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFG
GFASFIITWLIATTGSSMAPAIYVMIAAAISLAGTRLVREPAYLQQR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=437, Percent_Identity=34.324942791762, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788292, Length=440, Percent_Identity=30.6818181818182, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788942, Length=399, Percent_Identity=33.0827067669173, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=34.2723004694836, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=24.8704663212435, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082231, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48047; Mature: 48047

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNSATPTSATATATYSSTAPARSAAVIEPAQRRRAIIATVIGNGLEWFDFTVYSFFAII
CCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAKLFFPTGNDLSSLLLAVATFGVGFFMRPVGGIVLGVYADRVGRKAALSMTILLMALGT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATAFLTEYAPERERAYYSSWIQA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
SIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRET
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLNVPQSDGFVAGM
HHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHH
VGGCTIMLFAPLVGRLADRIGRRALLSGSALLILVLAWPMFAYINHVPGLSSLLVFQLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFIITWLIATTGSSMAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AIYVMIAAAISLAGTRLVREPAYLQQR
HHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MHNSATPTSATATATYSSTAPARSAAVIEPAQRRRAIIATVIGNGLEWFDFTVYSFFAII
CCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAKLFFPTGNDLSSLLLAVATFGVGFFMRPVGGIVLGVYADRVGRKAALSMTILLMALGT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLIGIAPTYEQMGIFAPLLIVVARLLQGFSAGGEMGGATAFLTEYAPERERAYYSSWIQA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
SIGVAVLLGAAVGTFVTRSLSPEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRET
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DERADSPLKEVFQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLNVPQSDGFVAGM
HHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHH
VGGCTIMLFAPLVGRLADRIGRRALLSGSALLILVLAWPMFAYINHVPGLSSLLVFQLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GILIAAYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFIITWLIATTGSSMAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AIYVMIAAAISLAGTRLVREPAYLQQR
HHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]