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The map label for this gene is dnaG [H]

Identifier: 73540590

GI number: 73540590

Start: 965208

End: 967022

Strand: Direct

Name: dnaG [H]

Synonym: Reut_A0887

Alternate gene names: 73540590

Gene position: 965208-967022 (Clockwise)

Preceding gene: 73540589

Following gene: 73540591

Centisome position: 25.36

GC content: 65.18

Gene sequence:

>1815_bases
GTGATTCCGCAATCCTTCATTCAGGACCTGCTCAACCGTGTCGACATTGTCGATGTGGTGGGCAAGTATGTGCAGCTGAA
GAAGGGCGGCGCCAACTTTATGGGGCTGTGCCCCTTCCATAACGAGAAATCGCCGTCGTTCACGGTGTCGCCGACCAAGC
AGTTCTATCACTGCTTTGGCTGCGGGGCGCACGGGTCGGCGATCGGTTTCCTGATGGAGTATTCGGGGCAGTCGTATCCG
GAGGCTGTCCGAGAGCTTGCCCAGTCCGTGGGCATGTCGGTCCCCGAGGAGCGCGACCGCCTGCCGCCGGGCCAGCGCGC
GGAACAGCAGGCGCGTTCGGTGGCGTTGTCCGACGCCATGAGCCGCGCCACGGAGTTCTACCGCCGCCAGCTGCGCGGCG
CACCGCAGGCGATCCAGTACCTGAAGGGGCGCGGCCTCACCGGCGAGATCGCGGCCCAGTTCGGCCTTGGCTACGCGCCG
GACGACTGGCAGGGTCTGGAAGCCGTCTTTGGCAGCTATCGCGATGACACGATTGCGGCGCCGCTTGTGGAGGCCGGCCT
GCTGATCGAAAGCAGCGAGAAGCGCGATGCGGACGGCAAGCCACGCCGCTACGACCGTTTCCGCGACCGCATCATGTTCC
CTATCCGCAACACCAAGGGTGCGGTGATCGGCTTCGGTGGGCGCGTCATAGGGCAGGGCGAGCCCAAGTACCTTAATTCG
CCCGAGACGCCTCTGTTCAGCAAAGGCACTGAACTGTACGGGCTGTTTGAGGCCCGTCACGCGATTCGGGAAACCGGCTA
CGTCCTCGTGGTCGAAGGGTATATGGATGTCGTGGCGCTGGCGCAGCTTGGGTTTGCCAATGCCGTGGCCACATTGGGCA
CGGCGTGCACGCCCGTGCACGTGCAAAAGCTTCTGCGCCAGACTGATTCGGTCGTGTTCTCGTTCGATGGGGACGCGGCG
GGCCGGCGCGCCGCGCGGCGCGCGCTCGAGGCGTGCCTCCCGCACGTGGCTGACAACAAGGCCATCCGCTTCCTGTTCCT
GCCGGCCGAGCACGATCCGGACAGCTATGTACGCGAAGAGGGTACCGAAGCGTTCGCTGCTCAGGTGCGCGACGCCATGC
CGTTGTCGCGGTTCCTGCTGCAGGCGGTGACGGAAGAGCAGGATATGCGTCAGCCGGAGGGTCGTGCCCGCGCACAGTAT
GAAGCCAAGCCACTGCTGCAGGCGATGCCGGCTTGCGGCCTGCGGCTACAGATTATCCGCGAACTTGCCGATGCTACAGG
GACAACACCGGCCGAGATCGAGGCGATTTGCGGGCTGCGCAGCGACCCGGCACGTGCCGGCCGCTTCGCCCAGCCGCGGC
CACGTGCGAAGCGCCAGGCACCGACCGGTCTCGAGCAGCGCGTGCTGCAATTGCTGATGTGCTATCCGTCCCTGGCGGCG
CGTCTTGACGAGGATAGCCGGGCACTGCTGCTTGATCCGGAGGGTGCGGACGCCGAAGTGCTGGCTCACCTAGTCGCTGC
CTGTGACGAGGTGGAAGGTCAGGTGAACTTTGCGGCCTTTAGCGAGCACCTGGCGCAATCGCCGTATGCGGAAATGTATG
CTGCGGCGCGCACAGCGGTGCTGCGTGAAGAAATTGAAGAGGAACCCGCGACGCAGGAATTCGACGCCGCGGTCACCAAG
CTGCTCGCTGAACCGCTGAGCCGCGAACTGGAAAGGCTGCAGGCGGCCGTGATTGCCGGTACCGCCGATGAGGATGGCAA
GCAGCGCATGCGCTGGCTGGTGGGTGAAATCCATCGCCGCCGCCGTCTCGGCTGA

Upstream 100 bases:

>100_bases
TACAATAGTTCGATAGCAACCGGGAGACTTTCCGGTCACGCATTCTTAAAAAGCGGCTGCCCGACCAAGGGAAGGCCATG
CCATCAGGACGGTCAGTCGG

Downstream 100 bases:

>100_bases
AGCAGGCTGAATGGTGCCGAGGGGGCATTTGCGCGGGGCAGCAAGGAAGATGTAGTAGGCTGAGCCAAGGCGGGGTCGAG
CTTAGCCCTTGATTTTTCAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP
EAVRELAQSVGMSVPEERDRLPPGQRAEQQARSVALSDAMSRATEFYRRQLRGAPQAIQYLKGRGLTGEIAAQFGLGYAP
DDWQGLEAVFGSYRDDTIAAPLVEAGLLIESSEKRDADGKPRRYDRFRDRIMFPIRNTKGAVIGFGGRVIGQGEPKYLNS
PETPLFSKGTELYGLFEARHAIRETGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQTDSVVFSFDGDAA
GRRAARRALEACLPHVADNKAIRFLFLPAEHDPDSYVREEGTEAFAAQVRDAMPLSRFLLQAVTEEQDMRQPEGRARAQY
EAKPLLQAMPACGLRLQIIRELADATGTTPAEIEAICGLRSDPARAGRFAQPRPRAKRQAPTGLEQRVLQLLMCYPSLAA
RLDEDSRALLLDPEGADAEVLAHLVAACDEVEGQVNFAAFSEHLAQSPYAEMYAAARTAVLREEIEEEPATQEFDAAVTK
LLAEPLSRELERLQAAVIAGTADEDGKQRMRWLVGEIHRRRRLG

Sequences:

>Translated_604_residues
MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP
EAVRELAQSVGMSVPEERDRLPPGQRAEQQARSVALSDAMSRATEFYRRQLRGAPQAIQYLKGRGLTGEIAAQFGLGYAP
DDWQGLEAVFGSYRDDTIAAPLVEAGLLIESSEKRDADGKPRRYDRFRDRIMFPIRNTKGAVIGFGGRVIGQGEPKYLNS
PETPLFSKGTELYGLFEARHAIRETGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQTDSVVFSFDGDAA
GRRAARRALEACLPHVADNKAIRFLFLPAEHDPDSYVREEGTEAFAAQVRDAMPLSRFLLQAVTEEQDMRQPEGRARAQY
EAKPLLQAMPACGLRLQIIRELADATGTTPAEIEAICGLRSDPARAGRFAQPRPRAKRQAPTGLEQRVLQLLMCYPSLAA
RLDEDSRALLLDPEGADAEVLAHLVAACDEVEGQVNFAAFSEHLAQSPYAEMYAAARTAVLREEIEEEPATQEFDAAVTK
LLAEPLSRELERLQAAVIAGTADEDGKQRMRWLVGEIHRRRRLG
>Mature_604_residues
MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGCGAHGSAIGFLMEYSGQSYP
EAVRELAQSVGMSVPEERDRLPPGQRAEQQARSVALSDAMSRATEFYRRQLRGAPQAIQYLKGRGLTGEIAAQFGLGYAP
DDWQGLEAVFGSYRDDTIAAPLVEAGLLIESSEKRDADGKPRRYDRFRDRIMFPIRNTKGAVIGFGGRVIGQGEPKYLNS
PETPLFSKGTELYGLFEARHAIRETGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVHVQKLLRQTDSVVFSFDGDAA
GRRAARRALEACLPHVADNKAIRFLFLPAEHDPDSYVREEGTEAFAAQVRDAMPLSRFLLQAVTEEQDMRQPEGRARAQY
EAKPLLQAMPACGLRLQIIRELADATGTTPAEIEAICGLRSDPARAGRFAQPRPRAKRQAPTGLEQRVLQLLMCYPSLAA
RLDEDSRALLLDPEGADAEVLAHLVAACDEVEGQVNFAAFSEHLAQSPYAEMYAAARTAVLREEIEEEPATQEFDAAVTK
LLAEPLSRELERLQAAVIAGTADEDGKQRMRWLVGEIHRRRRLG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=483, Percent_Identity=46.1697722567288, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66532; Mature: 66532

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGSAIGFLMEYSGQSYPEAVRELAQSVGMSVPEERDRLPPGQRAEQQARSVALSDAM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
SRATEFYRRQLRGAPQAIQYLKGRGLTGEIAAQFGLGYAPDDWQGLEAVFGSYRDDTIAA
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHH
PLVEAGLLIESSEKRDADGKPRRYDRFRDRIMFPIRNTKGAVIGFGGRVIGQGEPKYLNS
HHHHHHHHEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCCEEEECCEEECCCCCCCCCC
PETPLFSKGTELYGLFEARHAIRETGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVH
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VQKLLRQTDSVVFSFDGDAAGRRAARRALEACLPHVADNKAIRFLFLPAEHDPDSYVREE
HHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHC
GTEAFAAQVRDAMPLSRFLLQAVTEEQDMRQPEGRARAQYEAKPLLQAMPACGLRLQIIR
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ELADATGTTPAEIEAICGLRSDPARAGRFAQPRPRAKRQAPTGLEQRVLQLLMCYPSLAA
HHHHCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHH
RLDEDSRALLLDPEGADAEVLAHLVAACDEVEGQVNFAAFSEHLAQSPYAEMYAAARTAV
HCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LREEIEEEPATQEFDAAVTKLLAEPLSRELERLQAAVIAGTADEDGKQRMRWLVGEIHRR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RRLG
HCCC
>Mature Secondary Structure
MIPQSFIQDLLNRVDIVDVVGKYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEECCHHHHHHHHC
CGAHGSAIGFLMEYSGQSYPEAVRELAQSVGMSVPEERDRLPPGQRAEQQARSVALSDAM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
SRATEFYRRQLRGAPQAIQYLKGRGLTGEIAAQFGLGYAPDDWQGLEAVFGSYRDDTIAA
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHH
PLVEAGLLIESSEKRDADGKPRRYDRFRDRIMFPIRNTKGAVIGFGGRVIGQGEPKYLNS
HHHHHHHHEECCCCCCCCCCCHHHHHHHHHEEEEEECCCCCEEEECCEEECCCCCCCCCC
PETPLFSKGTELYGLFEARHAIRETGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVH
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VQKLLRQTDSVVFSFDGDAAGRRAARRALEACLPHVADNKAIRFLFLPAEHDPDSYVREE
HHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHC
GTEAFAAQVRDAMPLSRFLLQAVTEEQDMRQPEGRARAQYEAKPLLQAMPACGLRLQIIR
CHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
ELADATGTTPAEIEAICGLRSDPARAGRFAQPRPRAKRQAPTGLEQRVLQLLMCYPSLAA
HHHHCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHH
RLDEDSRALLLDPEGADAEVLAHLVAACDEVEGQVNFAAFSEHLAQSPYAEMYAAARTAV
HCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LREEIEEEPATQEFDAAVTKLLAEPLSRELERLQAAVIAGTADEDGKQRMRWLVGEIHRR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RRLG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]