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The map label for this gene is fabI2 [H]

Identifier: 73540334

GI number: 73540334

Start: 677101

End: 677880

Strand: Direct

Name: fabI2 [H]

Synonym: Reut_A0628

Alternate gene names: 73540334

Gene position: 677101-677880 (Clockwise)

Preceding gene: 73540333

Following gene: 73540335

Centisome position: 17.79

GC content: 66.79

Gene sequence:

>780_bases
ATGGTGCAAGTATCGAGTCCGCCGCTGGCCGGCGCGCGTGTGCTGATCGTCGGCGTCGCCAATGCCGATTCGATTGCCTG
GGGATGTGCGCGCGCATTCCGCGAACTGGGCGCGGAGATCGCGCTGACGTATCTCAACGACAAGGCCCATCCCCACGTCG
CGCCACTGGCCGAAGCGGTGCAGGCGCCGATCCTGATGCCGCTCAATGTCGAGGACGACACGCAGATGGACGCGCTGTTC
GCACGCATTGCCAGCGACTGGGGCCGGCTCGATGCCGTCGTGCACTCGGTCGCGTTTGCCCCGAAGGAAGACCTGCAGGG
CGGGCTGATCAACTCGTCCGCGGCAGGCTTCGCGCGTGCGATGGATATCTCGTGCCACTCGTTCATCCGCATGGCGCGGC
GCGCGGTGCCGCTGATGACCCAAGGCGGCACGCTGTTCGCCATGAGCTATGAGGGCGCGAACCGCGTGGTGCCCAACTAC
GACCTGATGGGGCCGGTCAAGGCCGCGCTGGAAGCGAGCTGCCGCTATCTCGCGCACGAACTCGGGCCGCGAGGTATCCG
CGTGCATGCGATTTCGCCGGGACCGCTCAAGACGCGCGCGGCATCGGGCCTGAAGGACTTTGACCTGATGCTTGCCGAGG
CCGCGGGACGTGCGCCGCTCGGCGAACTGGTCGACATCATGGACGTCGGCTTCGCGACGGCCTACCTGGCCACACCGTAT
GCACGGCGCATCTCCGGCAACACGATGTACGTGGATGGCGGCGTGCACATCATGGCCTGA

Upstream 100 bases:

>100_bases
GGTAACGCGTCCCGAAGCAAGCAACACGCAGACGACCGGCAAGCGCAACGCCGGTCCGCGCACTTAGCCATCACTGACTG
AAGCCTTCCGGGAGTTCGCC

Downstream 100 bases:

>100_bases
CGCGCGTAGGTTCGAGGGCCACGAAAGCCATGCAGATACTGATCCGCGGCCTGCACCGCGACGTGACCGAAGCGATGCTG
CGCGACATGCTCGCGCGGCA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MVQVSSPPLAGARVLIVGVANADSIAWGCARAFRELGAEIALTYLNDKAHPHVAPLAEAVQAPILMPLNVEDDTQMDALF
ARIASDWGRLDAVVHSVAFAPKEDLQGGLINSSAAGFARAMDISCHSFIRMARRAVPLMTQGGTLFAMSYEGANRVVPNY
DLMGPVKAALEASCRYLAHELGPRGIRVHAISPGPLKTRAASGLKDFDLMLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRISGNTMYVDGGVHIMA

Sequences:

>Translated_259_residues
MVQVSSPPLAGARVLIVGVANADSIAWGCARAFRELGAEIALTYLNDKAHPHVAPLAEAVQAPILMPLNVEDDTQMDALF
ARIASDWGRLDAVVHSVAFAPKEDLQGGLINSSAAGFARAMDISCHSFIRMARRAVPLMTQGGTLFAMSYEGANRVVPNY
DLMGPVKAALEASCRYLAHELGPRGIRVHAISPGPLKTRAASGLKDFDLMLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRISGNTMYVDGGVHIMA
>Mature_259_residues
MVQVSSPPLAGARVLIVGVANADSIAWGCARAFRELGAEIALTYLNDKAHPHVAPLAEAVQAPILMPLNVEDDTQMDALF
ARIASDWGRLDAVVHSVAFAPKEDLQGGLINSSAAGFARAMDISCHSFIRMARRAVPLMTQGGTLFAMSYEGANRVVPNY
DLMGPVKAALEASCRYLAHELGPRGIRVHAISPGPLKTRAASGLKDFDLMLAEAAGRAPLGELVDIMDVGFATAYLATPY
ARRISGNTMYVDGGVHIMA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=43.2539682539683, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1790717, Length=257, Percent_Identity=24.5136186770428, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644337, Length=177, Percent_Identity=28.8135593220339, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=24.7104247104247, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=24.609375, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27457; Mature: 27457

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVSSPPLAGARVLIVGVANADSIAWGCARAFRELGAEIALTYLNDKAHPHVAPLAEAV
CCEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHHHH
QAPILMPLNVEDDTQMDALFARIASDWGRLDAVVHSVAFAPKEDLQGGLINSSAAGFARA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHH
MDISCHSFIRMARRAVPLMTQGGTLFAMSYEGANRVVPNYDLMGPVKAALEASCRYLAHE
HHHHHHHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGPRGIRVHAISPGPLKTRAASGLKDFDLMLAEAAGRAPLGELVDIMDVGFATAYLATPY
CCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
ARRISGNTMYVDGGVHIMA
HHHCCCCEEEEECCEEEEC
>Mature Secondary Structure
MVQVSSPPLAGARVLIVGVANADSIAWGCARAFRELGAEIALTYLNDKAHPHVAPLAEAV
CCEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHHHH
QAPILMPLNVEDDTQMDALFARIASDWGRLDAVVHSVAFAPKEDLQGGLINSSAAGFARA
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHH
MDISCHSFIRMARRAVPLMTQGGTLFAMSYEGANRVVPNYDLMGPVKAALEASCRYLAHE
HHHHHHHHHHHHHHHCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGPRGIRVHAISPGPLKTRAASGLKDFDLMLAEAAGRAPLGELVDIMDVGFATAYLATPY
CCCCCEEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
ARRISGNTMYVDGGVHIMA
HHHCCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]