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The map label for this gene is lptD

Identifier: 73540203

GI number: 73540203

Start: 539257

End: 541689

Strand: Direct

Name: lptD

Synonym: Reut_A0497

Alternate gene names: 73540203

Gene position: 539257-541689 (Clockwise)

Preceding gene: 73540185

Following gene: 73540204

Centisome position: 14.17

GC content: 64.0

Gene sequence:

>2433_bases
ATGACCGAACAACGACGATCACCGAACAAACGAGCCAACAAGCCGCCTGCGCTTTCCGGCCTATCCAGCCGCACCGGCCG
GCTGCCTGCCAGCGCCCTGCGGCCGCTGGTGCTGGCCATGGCCGGCCTGTCGGTGGGCGCGCACGCCCAGATGTCCAGTT
CGGCGGTGCCAGACATCGATCAGACCGAGTCCGTGGTGGAAACCGCCCCGGAAGTGCCGTTGGCGCCGCCTGTGTCCGGC
GAGCTCGTCCCGCGCCTGGCCGAGCCCGCGAAGACCACCCAGCCTGGGGAAAGCCCGCCAGCATTCGTGTCGGGCGACCG
GCTCACCGGCTACAACGAGCGTGGCGTAGTGCTCGAAGGCAATGCCGAATTGCGCCGGGGCGGCGCCGTGGTCAAGGGCG
ACAAGCTGACCTACGACCAGGACACCGACGAAGCCTTCGCGCATGGCAATGCCCGTGTGTCGCGCGGCGGCGCGCTGGCC
GTGGGCCCCGAGGCTCGTATGAAGGTCGAGGCGAACGAGGGCTACATGCTCTCGCCCGACTACTACTTCCAGCAGACCGG
CGGTACGGGCAAGGCCGAACGCATCGATTTCATCGACAAGGACCGCAGTGTGATCAAGCGGGCCACGTACACGACGTGCT
CGCCGGACAACGCCGACTGGTACTTCAGCGCGCGCCAGCTCGACCTGGACAGCGATCGCGAAGTGGGCGTGGCCTACGGC
GGCGTGCTGCATTTCTTCGACGTACCGATCGCGGGCGCCCCGGTGTTCAGCTTCCCGCTCACCGACGAGCGCCGCAGCGG
TTTCCTGTCGCCGCTGTTCGGCTACGGGTCGAAATCGGGCCTCGACGTCACGCTCCCGTACTACTTCAACATCGCGCCGA
ACCGTGACCTGACGCTGTATCCGCGCATCATGACGTCGCGTGGTTTCATGCTCGGCGGCGACTACCGGTACATCGGCCAG
GGATATTCGGGCCGGCTGCGCGCTGAAATCCTGCCGGATGACCGTCAGACGAATTCGAACCGCTGGTCCTATTCGTTCCA
GCATACCCAGAACCTGGCGAAAGGGCTTAACGCTTACCTGAACCTGAACCGCGTATCGGACGACCGGTATCCGGACGATC
TGACGCGTACCGTGGCGCAATCGACGCAGCGGCAGTACATCCAGGAAGGTGGTGTCACCTATAACTGGCAGGACTTCACG
GTCCTCGCGCGCGTACAGAAGTTCCAGACGCTGCTGCCGAGCGGGCCGTCCTACGAGCGCGAGCCGCAGCTCAACGGCAA
GTACATCCGCTATGACCTCGGCGGCTTCGACGTGCAGGTGGAGGCCGACTACACGCGTTTCCGCATCCCGCTGACCTCGA
CCGGCTTCCAGCAGCCGCAGGGCGAGCGCATGTTCGTGCAGCCGACCATCAGCTATCCGATCGTGCGGCCGGGCTGGTAC
GCGACGCCCAAGGTCATCTTCAATGCGACGCAGTACCAGATGGAGCCGCAAAGCAATACGCCGACGGCGCAGAACAACTT
CTCTCGCACGGTACCGACATTCAGTTTCGATTCGGGCATGACCTTCGAGCGCGACGCGCCGACCATGAGCCGGGTGTTCG
GGGTCAACTACCTGCAGACGCTCGAGCCGCGGCTGTTCTACGTCTACACGCCGTTCCGCGACCAGAGCCAGTTTCCGCTG
TTCGATACCGTGCAGTCGGACTTCGGTTACGGCCAGATCTTTTCCGAGAACCCGTTCACCGGCTATGACCGGGTGGCCGA
CAACAATAAGCTGACTGCGGGCCTGACCACGCGTTTGATCGAGGCCGACACCGGCATCGAGCGTTTCCGCGGCACCATCG
CGCAACGCTACGACTTCAGTGGCCAGCGCGTGCAGATCAACGGCACGCTGCCGGATCCGAAGGCCGGGAGCTCCGACCTG
CTGGCCGCCACGACCATTCAGCTGTTCCGTGGTTACTACCTGGATGCAGGCATCCAATACAATCCGGATTCGGAACACAT
CAACTACTCGAACCTGGCGTTCGCGTGGCGTCCGGAGCCGCGCAAGCTGCTCAACGTGGGCTACCGCTACCGGCGGGCCA
CGTCGGTCACGGACAACACCGCCATCGACCAGTTCGAGATTTCCGGTCAGTGGCCCATTACCCAGCGCGCCTATGGCATA
GGCCGTTTCGCGTTCGACCGCAATGCGAACCAGATGGTCGATGCGCTCGCCGGCCTGGAATACGCGGCTGACTGCTGGGT
GGGCCGCGTGGTCTACCAGCGCTACCGCAATACCACGCAGGGCTATACCGGCCGGATCTTCCTGCAGGTGGAGTTCCGCG
GACTGTCGAAGATCGGCTCGAATCCGCTTGATATCCTGCGCCTGAATGTTCCTGGGTACGAACCTGTCACCGCCAAGCCG
GTGCCGACGACCCAGTTTGATCACTATGAATGA

Upstream 100 bases:

>100_bases
CCCCCGGGAGGGGCTCACCGTTGCCCCGCAGCCTGTTCCGTGGGGGGCTGGCGGCTAGCAGGCAAGCCGTCGCCGCCGAC
GTGACTGACAACCCGCTCGC

Downstream 100 bases:

>100_bases
CGGATTACGATGAAACGTCAAGAATTCGCCTTGTTTTCCCTGACCCTGATGTTGTCGCCGTGGCGCCGCGTTCTGCTGCC
GGCCGTGCTCGCGGCCATGG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTEQRRSPNKRANKPPALSGLSSRTGRLPASALRPLVLAMAGLSVGAHAQMSSSAVPDIDQTESVVETAPEVPLAPPVSG
ELVPRLAEPAKTTQPGESPPAFVSGDRLTGYNERGVVLEGNAELRRGGAVVKGDKLTYDQDTDEAFAHGNARVSRGGALA
VGPEARMKVEANEGYMLSPDYYFQQTGGTGKAERIDFIDKDRSVIKRATYTTCSPDNADWYFSARQLDLDSDREVGVAYG
GVLHFFDVPIAGAPVFSFPLTDERRSGFLSPLFGYGSKSGLDVTLPYYFNIAPNRDLTLYPRIMTSRGFMLGGDYRYIGQ
GYSGRLRAEILPDDRQTNSNRWSYSFQHTQNLAKGLNAYLNLNRVSDDRYPDDLTRTVAQSTQRQYIQEGGVTYNWQDFT
VLARVQKFQTLLPSGPSYEREPQLNGKYIRYDLGGFDVQVEADYTRFRIPLTSTGFQQPQGERMFVQPTISYPIVRPGWY
ATPKVIFNATQYQMEPQSNTPTAQNNFSRTVPTFSFDSGMTFERDAPTMSRVFGVNYLQTLEPRLFYVYTPFRDQSQFPL
FDTVQSDFGYGQIFSENPFTGYDRVADNNKLTAGLTTRLIEADTGIERFRGTIAQRYDFSGQRVQINGTLPDPKAGSSDL
LAATTIQLFRGYYLDAGIQYNPDSEHINYSNLAFAWRPEPRKLLNVGYRYRRATSVTDNTAIDQFEISGQWPITQRAYGI
GRFAFDRNANQMVDALAGLEYAADCWVGRVVYQRYRNTTQGYTGRIFLQVEFRGLSKIGSNPLDILRLNVPGYEPVTAKP
VPTTQFDHYE

Sequences:

>Translated_810_residues
MTEQRRSPNKRANKPPALSGLSSRTGRLPASALRPLVLAMAGLSVGAHAQMSSSAVPDIDQTESVVETAPEVPLAPPVSG
ELVPRLAEPAKTTQPGESPPAFVSGDRLTGYNERGVVLEGNAELRRGGAVVKGDKLTYDQDTDEAFAHGNARVSRGGALA
VGPEARMKVEANEGYMLSPDYYFQQTGGTGKAERIDFIDKDRSVIKRATYTTCSPDNADWYFSARQLDLDSDREVGVAYG
GVLHFFDVPIAGAPVFSFPLTDERRSGFLSPLFGYGSKSGLDVTLPYYFNIAPNRDLTLYPRIMTSRGFMLGGDYRYIGQ
GYSGRLRAEILPDDRQTNSNRWSYSFQHTQNLAKGLNAYLNLNRVSDDRYPDDLTRTVAQSTQRQYIQEGGVTYNWQDFT
VLARVQKFQTLLPSGPSYEREPQLNGKYIRYDLGGFDVQVEADYTRFRIPLTSTGFQQPQGERMFVQPTISYPIVRPGWY
ATPKVIFNATQYQMEPQSNTPTAQNNFSRTVPTFSFDSGMTFERDAPTMSRVFGVNYLQTLEPRLFYVYTPFRDQSQFPL
FDTVQSDFGYGQIFSENPFTGYDRVADNNKLTAGLTTRLIEADTGIERFRGTIAQRYDFSGQRVQINGTLPDPKAGSSDL
LAATTIQLFRGYYLDAGIQYNPDSEHINYSNLAFAWRPEPRKLLNVGYRYRRATSVTDNTAIDQFEISGQWPITQRAYGI
GRFAFDRNANQMVDALAGLEYAADCWVGRVVYQRYRNTTQGYTGRIFLQVEFRGLSKIGSNPLDILRLNVPGYEPVTAKP
VPTTQFDHYE
>Mature_809_residues
TEQRRSPNKRANKPPALSGLSSRTGRLPASALRPLVLAMAGLSVGAHAQMSSSAVPDIDQTESVVETAPEVPLAPPVSGE
LVPRLAEPAKTTQPGESPPAFVSGDRLTGYNERGVVLEGNAELRRGGAVVKGDKLTYDQDTDEAFAHGNARVSRGGALAV
GPEARMKVEANEGYMLSPDYYFQQTGGTGKAERIDFIDKDRSVIKRATYTTCSPDNADWYFSARQLDLDSDREVGVAYGG
VLHFFDVPIAGAPVFSFPLTDERRSGFLSPLFGYGSKSGLDVTLPYYFNIAPNRDLTLYPRIMTSRGFMLGGDYRYIGQG
YSGRLRAEILPDDRQTNSNRWSYSFQHTQNLAKGLNAYLNLNRVSDDRYPDDLTRTVAQSTQRQYIQEGGVTYNWQDFTV
LARVQKFQTLLPSGPSYEREPQLNGKYIRYDLGGFDVQVEADYTRFRIPLTSTGFQQPQGERMFVQPTISYPIVRPGWYA
TPKVIFNATQYQMEPQSNTPTAQNNFSRTVPTFSFDSGMTFERDAPTMSRVFGVNYLQTLEPRLFYVYTPFRDQSQFPLF
DTVQSDFGYGQIFSENPFTGYDRVADNNKLTAGLTTRLIEADTGIERFRGTIAQRYDFSGQRVQINGTLPDPKAGSSDLL
AATTIQLFRGYYLDAGIQYNPDSEHINYSNLAFAWRPEPRKLLNVGYRYRRATSVTDNTAIDQFEISGQWPITQRAYGIG
RFAFDRNANQMVDALAGLEYAADCWVGRVVYQRYRNTTQGYTGRIFLQVEFRGLSKIGSNPLDILRLNVPGYEPVTAKPV
PTTQFDHYE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=742, Percent_Identity=27.3584905660377, Blast_Score=273, Evalue=2e-74,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_CUPPJ (Q475Q4)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294723.1
- GeneID:   3611801
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0497
- NMPDR:   fig|264198.3.peg.1009
- HOGENOM:   HBG701380
- OMA:   TPYYWNI
- ProtClustDB:   CLSK2302405
- BioCyc:   REUT264198:REUT_A0497-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 90727; Mature: 90596

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQRRSPNKRANKPPALSGLSSRTGRLPASALRPLVLAMAGLSVGAHAQMSSSAVPDID
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
QTESVVETAPEVPLAPPVSGELVPRLAEPAKTTQPGESPPAFVSGDRLTGYNERGVVLEG
HHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEC
NAELRRGGAVVKGDKLTYDQDTDEAFAHGNARVSRGGALAVGPEARMKVEANEGYMLSPD
CCHHHCCCCEEECCEEECCCCCCHHHHCCCCEEECCCEEEECCCCEEEEECCCCEEECCC
YYFQQTGGTGKAERIDFIDKDRSVIKRATYTTCSPDNADWYFSARQLDLDSDREVGVAYG
CEEEECCCCCCCCEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCEEEEC
GVLHFFDVPIAGAPVFSFPLTDERRSGFLSPLFGYGSKSGLDVTLPYYFNIAPNRDLTLY
CEEEEECCCCCCCCEEECCCCCHHHCCCCCHHHCCCCCCCCEEEEEEEEEECCCCCEEEE
PRIMTSRGFMLGGDYRYIGQGYSGRLRAEILPDDRQTNSNRWSYSFQHTQNLAKGLNAYL
EEECCCCCEEECCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHEE
NLNRVSDDRYPDDLTRTVAQSTQRQYIQEGGVTYNWQDFTVLARVQKFQTLLPSGPSYER
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCCCCCC
EPQLNGKYIRYDLGGFDVQVEADYTRFRIPLTSTGFQQPQGERMFVQPTISYPIVRPGWY
CCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEECCCCC
ATPKVIFNATQYQMEPQSNTPTAQNNFSRTVPTFSFDSGMTFERDAPTMSRVFGVNYLQT
CCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCEECCCCCEECCCCCHHHHHHCCHHHHH
LEPRLFYVYTPFRDQSQFPLFDTVQSDFGYGQIFSENPFTGYDRVADNNKLTAGLTTRLI
CCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCHHHCCCCEEEECCEEEEE
EADTGIERFRGTIAQRYDFSGQRVQINGTLPDPKAGSSDLLAATTIQLFRGYYLDAGIQY
ECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCEEEECCEEE
NPDSEHINYSNLAFAWRPEPRKLLNVGYRYRRATSVTDNTAIDQFEISGQWPITQRAYGI
CCCCCCCCCCCEEEEECCCHHHHHHHCHHHEEECCCCCCCCCCEEEECCCCCCHHHHCCC
GRFAFDRNANQMVDALAGLEYAADCWVGRVVYQRYRNTTQGYTGRIFLQVEFRGLSKIGS
EEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHCC
NPLDILRLNVPGYEPVTAKPVPTTQFDHYE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEQRRSPNKRANKPPALSGLSSRTGRLPASALRPLVLAMAGLSVGAHAQMSSSAVPDID
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
QTESVVETAPEVPLAPPVSGELVPRLAEPAKTTQPGESPPAFVSGDRLTGYNERGVVLEG
HHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEC
NAELRRGGAVVKGDKLTYDQDTDEAFAHGNARVSRGGALAVGPEARMKVEANEGYMLSPD
CCHHHCCCCEEECCEEECCCCCCHHHHCCCCEEECCCEEEECCCCEEEEECCCCEEECCC
YYFQQTGGTGKAERIDFIDKDRSVIKRATYTTCSPDNADWYFSARQLDLDSDREVGVAYG
CEEEECCCCCCCCEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCEEEEC
GVLHFFDVPIAGAPVFSFPLTDERRSGFLSPLFGYGSKSGLDVTLPYYFNIAPNRDLTLY
CEEEEECCCCCCCCEEECCCCCHHHCCCCCHHHCCCCCCCCEEEEEEEEEECCCCCEEEE
PRIMTSRGFMLGGDYRYIGQGYSGRLRAEILPDDRQTNSNRWSYSFQHTQNLAKGLNAYL
EEECCCCCEEECCCEEEECCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHEE
NLNRVSDDRYPDDLTRTVAQSTQRQYIQEGGVTYNWQDFTVLARVQKFQTLLPSGPSYER
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCCCCCC
EPQLNGKYIRYDLGGFDVQVEADYTRFRIPLTSTGFQQPQGERMFVQPTISYPIVRPGWY
CCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEECCCCC
ATPKVIFNATQYQMEPQSNTPTAQNNFSRTVPTFSFDSGMTFERDAPTMSRVFGVNYLQT
CCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCEECCCCCEECCCCCHHHHHHCCHHHHH
LEPRLFYVYTPFRDQSQFPLFDTVQSDFGYGQIFSENPFTGYDRVADNNKLTAGLTTRLI
CCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCHHHCCCCEEEECCEEEEE
EADTGIERFRGTIAQRYDFSGQRVQINGTLPDPKAGSSDLLAATTIQLFRGYYLDAGIQY
ECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCEEEECCEEE
NPDSEHINYSNLAFAWRPEPRKLLNVGYRYRRATSVTDNTAIDQFEISGQWPITQRAYGI
CCCCCCCCCCCEEEEECCCHHHHHHHCHHHEEECCCCCCCCCCEEEECCCCCCHHHHCCC
GRFAFDRNANQMVDALAGLEYAADCWVGRVVYQRYRNTTQGYTGRIFLQVEFRGLSKIGS
EEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHCC
NPLDILRLNVPGYEPVTAKPVPTTQFDHYE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA