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The map label for this gene is gatB [H]

Identifier: 73539777

GI number: 73539777

Start: 89061

End: 90518

Strand: Reverse

Name: gatB [H]

Synonym: Reut_A0071

Alternate gene names: 73539777

Gene position: 90518-89061 (Counterclockwise)

Preceding gene: 73539778

Following gene: 73539770

Centisome position: 2.38

GC content: 62.83

Gene sequence:

>1458_bases
ATGCAATGGGAAGTGGTGATCGGCCTCGAAACGCACACGCAGCTCTCGACGGCCTCCAAGATTTTCTCCGGGACCTCCAC
CGCCTTCGGCGCCGAGCCCAACACGCAGGCCTCGCCGGTTGACCTCGCGCTGCCGGGCGTGCTGCCTGTGCTGAACAAGG
GCGCGGTAGAACGCGCCATCCAGTTCGGCCTGGCGATCGGCGCCACCATCGCGCCGCGCAGCATCTTCGCGCGCAAGAAC
TACTTCTACCCCGACCTGCCCAAGGGCTACCAGATCAGCCAGTACGAGATTCCTGTGGTGCAGGGCGGCAGCATCACCAT
CCAGGTCGAAGGCAAGAAGGGCGAGGTGTACGAGAAGACCGTGCAGCTCACGCGCGCGCACCTGGAAGAAGACGCGGGCA
AGTCGCTCCACGAAGACTTCGCCGGCATGACCGGCATCGACCTGAACCGCGCCGGCACGCCGCTGCTCGAAATTGTGACC
GAGCCGGACATGCGCAGTGCGGCCGAGGCCGTAGCCTATGCCAAGGCGCTGCACTCGCTGGTGGTGTGGCTGGGCATCTG
CGACGGCAACATGCAGGAAGGCAGCTTCCGCTGCGACGCCAACGTCTCGGTGCGCCCCGTTGGCCAGAAGGAATTCGGCA
CGCGCCGCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTGCAACAGGCCATCGAATACGAGGTGCAGTGGCAGATCAAC
GAGATCGAGGATGGCCGCAAGATCCAGCAGGCCACCGTGCTGTTCGATCCGGACACCGGCGAAACGCGCGCGATGCGCAC
CAAGGAAGACGCGCACGACTATCGCTACTTCCCGGACCCGGACCTGATGCCGCTTGAGATCGACGCAGCCTGGATCGAAC
GTGTGCGCGGTGAGCTGCCCGAATTGCCGGCGGCCATGCAAGCGCGTTTCGTTTCGCAATATGGCCTGTCGGCGTATGAC
GCCAGCACGCTGACGGCTTCGAAGGCGATGGCCAGCTACTACGAAGCTGTCGTCGCCGAGGTCGGTGCGGCCAATGCCAA
GCCGGCAGCCAACTGGCTGATGGGCGATGTGGCGTCGCAGCTCAACCGTGAAGGCATCAGCATCGATGCCGCGCCGGTGA
AGCCTTTGCAACTGGCCAGGCTGCTGGCGCGCATTGCCGATGGCACGGTGTCCAACAACACCGCCAAGAAGGATGTCTTC
CCGGCCATGTGGGCAGGTGAGTCCAATGGCGATGCGGATGCGATCATCGCCGCCAAGGGACTGAAGCAGATGTCCGATAC
CGGCGAGCTCGAGAAGATCATCGATGACGTGCTGGCGGCCAATGCCAAGTCAGTGGAAGAGTTCCGGGCCGGAAAGGAGA
AGGCGTTCAATGCGCTGGTTGGACAGGCAATGAAGGCAACCAAGGGAAAGGCTAATCCGGCGCAGGTGAATGAGTTGTTG
AAGAAGAAATTGGGGTAA

Upstream 100 bases:

>100_bases
CCCCTGAGAGCGCCGGCAGTACAGACACAGACAATCGGACCGGTGCCAGCTCTGGTCCATCGCAACAGACAGAACCCAAG
AACCGAACAGGAATACCGCC

Downstream 100 bases:

>100_bases
GCGGTTTTGGTGATGCGGCGGCGTGTGCCTGGCATGCGCCACCGCCTCTATGCCAAAAGAAAATCAGCCCGAATCCCTTC
CCCCACCTCCCTGACCTTCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNKGAVERAIQFGLAIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGSITIQVEGKKGEVYEKTVQLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVT
EPDMRSAAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRREIKNLNSFRFLQQAIEYEVQWQIN
EIEDGRKIQQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWIERVRGELPELPAAMQARFVSQYGLSAYD
ASTLTASKAMASYYEAVVAEVGAANAKPAANWLMGDVASQLNREGISIDAAPVKPLQLARLLARIADGTVSNNTAKKDVF
PAMWAGESNGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELL
KKKLG

Sequences:

>Translated_485_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNKGAVERAIQFGLAIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGSITIQVEGKKGEVYEKTVQLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVT
EPDMRSAAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRREIKNLNSFRFLQQAIEYEVQWQIN
EIEDGRKIQQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWIERVRGELPELPAAMQARFVSQYGLSAYD
ASTLTASKAMASYYEAVVAEVGAANAKPAANWLMGDVASQLNREGISIDAAPVKPLQLARLLARIADGTVSNNTAKKDVF
PAMWAGESNGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELL
KKKLG
>Mature_485_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNKGAVERAIQFGLAIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGSITIQVEGKKGEVYEKTVQLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVT
EPDMRSAAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRREIKNLNSFRFLQQAIEYEVQWQIN
EIEDGRKIQQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWIERVRGELPELPAAMQARFVSQYGLSAYD
ASTLTASKAMASYYEAVVAEVGAANAKPAANWLMGDVASQLNREGISIDAAPVKPLQLARLLARIADGTVSNNTAKKDVF
PAMWAGESNGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELL
KKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=39.5582329317269, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=464, Percent_Identity=30.8189655172414, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=31.5384615384615, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=34.2799188640974, Blast_Score=309, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52654; Mature: 52654

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNKGAVERAI
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCHHHHHHHH
QFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGSITIQVEGKKGEVYEKT
HHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHHHH
VQLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSAAEAVAYAKALHSL
HHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
VVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRREIKNLNSFRFLQQAIEYEVQWQIN
HHHHHCCCCCCCCCCEEECCCCEEEECCCHHCCCHHHHHCCHHHHHHHHHHCEEEEEEEE
EIEDGRKIQQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWIERVRGELP
CCCCCCEEEEEEEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHCCCC
ELPAAMQARFVSQYGLSAYDASTLTASKAMASYYEAVVAEVGAANAKPAANWLMGDVASQ
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LNREGISIDAAPVKPLQLARLLARIADGTVSNNTAKKDVFPAMWAGESNGDADAIIAAKG
HCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCHHCCCCCCCCCHHHHHHHH
LKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELL
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKKLG
HHHCC
>Mature Secondary Structure
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNKGAVERAI
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCHHHHHHHH
QFGLAIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGSITIQVEGKKGEVYEKT
HHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHHHH
VQLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSAAEAVAYAKALHSL
HHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
VVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRREIKNLNSFRFLQQAIEYEVQWQIN
HHHHHCCCCCCCCCCEEECCCCEEEECCCHHCCCHHHHHCCHHHHHHHHHHCEEEEEEEE
EIEDGRKIQQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWIERVRGELP
CCCCCCEEEEEEEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHCCCC
ELPAAMQARFVSQYGLSAYDASTLTASKAMASYYEAVVAEVGAANAKPAANWLMGDVASQ
CCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LNREGISIDAAPVKPLQLARLLARIADGTVSNNTAKKDVFPAMWAGESNGDADAIIAAKG
HCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCHHCCCCCCCCCHHHHHHHH
LKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELL
HHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKKLG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA