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The map label for this gene is estB [H]

Identifier: 73539753

GI number: 73539753

Start: 61040

End: 62233

Strand: Reverse

Name: estB [H]

Synonym: Reut_A0047

Alternate gene names: 73539753

Gene position: 62233-61040 (Counterclockwise)

Preceding gene: 73539754

Following gene: 73539752

Centisome position: 1.63

GC content: 66.83

Gene sequence:

>1194_bases
ATGACCCGCATTTCCCGCCTGCTGGCCGGCGCCGTGCTTGCGCTGGGCGCCCTGCAAGTCCACGCCGCTCCGGCGGCGCC
CGCTCGCCCGGCCGATGGCATTCCGAGTGCGCAGCCTGAAGCGGTCGGCGTCGATTCAGCGAAACTCGTTGCGCTGTCCG
AATGGATTCGCCGCGACAAGCTCGACGTGCGCAGCCTGCTCGTCATCAAGGACGGCAAGCTCGTGTTCGAGCGCTACGGC
GACGGCCTCTCGCGTGACTACAACCACGAGTTGTACTCCGTCACGAAATTCATCAGCGCACTGCTGGTTGGCACACTGGT
CGGCGACGGCAAGCTCAGCGCCGAAGACACGCCCGCGCAGCGCCTCGCGGCTGCGCGCCCCGACCTGGCCCCTGCTCTCG
CCGACAAGAAAGCAATCCAGCTGCGCCATCTGATGTCGATGTCGAGTGGCCTGTCGTACAAGCTTGTGGAAGGCACCGAC
ACGCTGTACTACGGCGTGCCCGACCGGCTCAAGGTCGCGGCGTCCGCCAGCGTGCGTGTCGCGCCCGGCAAGGAGTTCGA
CTACATCGACGTGAACCCGGTGCTGGTCGGCGCCACCGTCAGCCAGGTCACGGGGCTGCCCGAACAGCAATACGCCGAAC
AGCGCCTGTTCAAGCCGCTCGGCATGCAGCACTACCGCTGGGACGGCGCCGACGGCAAGGGCGCTGTGTCCGGCGGCTGG
GGCCTGCGCCTGCGCTCGATCGACATGGCGCGGCTCGGCATGCTGATGCTGAACCAGGGCCGCTGGAACGGCCAGCAGGT
CGTGCCGGCCTCATGGGTCGCGCGCATGACCACGCCGTCCGGCCCGGCGCCGGACTACGGCTTCTACTGCTGGATCAACA
ACATCGTAAAGAGCGAGCGCGAGTTCAGCGCCATGGGCTACAAGGGCCAGTTCATCACTGTGCTGCCCAGGCAGAACGCC
GTCGTCGTGATGAACAGCATCATGTCCACGCAAGGCGGCCTGCGTGACGCGGCGTACCTCGACCTGTACCGGCGCATGGT
GAACGACTATTTGCTACCCGCGCTGCAGGCCGGCAATACCGTGCATCCCGATGCAGCCCGGCAGGCCGCGCTGCAGAAGG
AGCTCGAACTGGCACACACGAGCCAGCGCGTGCCCGGCACCGCGCTCGCCTTCAACGACAAGCCCGAACAGTAA

Upstream 100 bases:

>100_bases
TACGAGACCAAGATCCCGGTCTGACGACCTTCCCCCAGCAACGCTGTATCCGCCGCGCCGGTCCGTCCGACGGTCCACGC
GGCGGTGGAGGAGACACACC

Downstream 100 bases:

>100_bases
GCCACTACACAACAACTTGCGAGACGCCGATGACGGCGTCCGCACCACTCCTGAGAACACCTCCAATGACCAAGCAAATC
CGCCTCGGCATGCTGACGCC

Product: Beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTRISRLLAGAVLALGALQVHAAPAAPARPADGIPSAQPEAVGVDSAKLVALSEWIRRDKLDVRSLLVIKDGKLVFERYG
DGLSRDYNHELYSVTKFISALLVGTLVGDGKLSAEDTPAQRLAAARPDLAPALADKKAIQLRHLMSMSSGLSYKLVEGTD
TLYYGVPDRLKVAASASVRVAPGKEFDYIDVNPVLVGATVSQVTGLPEQQYAEQRLFKPLGMQHYRWDGADGKGAVSGGW
GLRLRSIDMARLGMLMLNQGRWNGQQVVPASWVARMTTPSGPAPDYGFYCWINNIVKSEREFSAMGYKGQFITVLPRQNA
VVVMNSIMSTQGGLRDAAYLDLYRRMVNDYLLPALQAGNTVHPDAARQAALQKELELAHTSQRVPGTALAFNDKPEQ

Sequences:

>Translated_397_residues
MTRISRLLAGAVLALGALQVHAAPAAPARPADGIPSAQPEAVGVDSAKLVALSEWIRRDKLDVRSLLVIKDGKLVFERYG
DGLSRDYNHELYSVTKFISALLVGTLVGDGKLSAEDTPAQRLAAARPDLAPALADKKAIQLRHLMSMSSGLSYKLVEGTD
TLYYGVPDRLKVAASASVRVAPGKEFDYIDVNPVLVGATVSQVTGLPEQQYAEQRLFKPLGMQHYRWDGADGKGAVSGGW
GLRLRSIDMARLGMLMLNQGRWNGQQVVPASWVARMTTPSGPAPDYGFYCWINNIVKSEREFSAMGYKGQFITVLPRQNA
VVVMNSIMSTQGGLRDAAYLDLYRRMVNDYLLPALQAGNTVHPDAARQAALQKELELAHTSQRVPGTALAFNDKPEQ
>Mature_396_residues
TRISRLLAGAVLALGALQVHAAPAAPARPADGIPSAQPEAVGVDSAKLVALSEWIRRDKLDVRSLLVIKDGKLVFERYGD
GLSRDYNHELYSVTKFISALLVGTLVGDGKLSAEDTPAQRLAAARPDLAPALADKKAIQLRHLMSMSSGLSYKLVEGTDT
LYYGVPDRLKVAASASVRVAPGKEFDYIDVNPVLVGATVSQVTGLPEQQYAEQRLFKPLGMQHYRWDGADGKGAVSGGWG
LRLRSIDMARLGMLMLNQGRWNGQQVVPASWVARMTTPSGPAPDYGFYCWINNIVKSEREFSAMGYKGQFITVLPRQNAV
VVMNSIMSTQGGLRDAAYLDLYRRMVNDYLLPALQAGNTVHPDAARQAALQKELELAHTSQRVPGTALAFNDKPEQ

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=398, Percent_Identity=24.1206030150754, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 43132; Mature: 43001

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRISRLLAGAVLALGALQVHAAPAAPARPADGIPSAQPEAVGVDSAKLVALSEWIRRDK
CCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
LDVRSLLVIKDGKLVFERYGDGLSRDYNHELYSVTKFISALLVGTLVGDGKLSAEDTPAQ
CCCEEEEEEECCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RLAAARPDLAPALADKKAIQLRHLMSMSSGLSYKLVEGTDTLYYGVPDRLKVAASASVRV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCHHHEEEECCEEEE
APGKEFDYIDVNPVLVGATVSQVTGLPEQQYAEQRLFKPLGMQHYRWDGADGKGAVSGGW
CCCCCCCEEECCCEEEECCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCC
GLRLRSIDMARLGMLMLNQGRWNGQQVVPASWVARMTTPSGPAPDYGFYCWINNIVKSER
CEEEEECCHHHHHHHHHCCCCCCCCEECCHHHHHEECCCCCCCCCCCEEEEHHHHHHCHH
EFSAMGYKGQFITVLPRQNAVVVMNSIMSTQGGLRDAAYLDLYRRMVNDYLLPALQAGNT
HHHHCCCCCCEEEEECCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
VHPDAARQAALQKELELAHTSQRVPGTALAFNDKPEQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TRISRLLAGAVLALGALQVHAAPAAPARPADGIPSAQPEAVGVDSAKLVALSEWIRRDK
CHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
LDVRSLLVIKDGKLVFERYGDGLSRDYNHELYSVTKFISALLVGTLVGDGKLSAEDTPAQ
CCCEEEEEEECCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RLAAARPDLAPALADKKAIQLRHLMSMSSGLSYKLVEGTDTLYYGVPDRLKVAASASVRV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCHHHEEEECCEEEE
APGKEFDYIDVNPVLVGATVSQVTGLPEQQYAEQRLFKPLGMQHYRWDGADGKGAVSGGW
CCCCCCCEEECCCEEEECCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCC
GLRLRSIDMARLGMLMLNQGRWNGQQVVPASWVARMTTPSGPAPDYGFYCWINNIVKSER
CEEEEECCHHHHHHHHHCCCCCCCCEECCHHHHHEECCCCCCCCCCCEEEEHHHHHHCHH
EFSAMGYKGQFITVLPRQNAVVVMNSIMSTQGGLRDAAYLDLYRRMVNDYLLPALQAGNT
HHHHCCCCCCEEEEECCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
VHPDAARQAALQKELELAHTSQRVPGTALAFNDKPEQ
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]