Click here to switch to the map view.

The map label for this gene is hyuA [H]

Identifier: 73539751

GI number: 73539751

Start: 58788

End: 60209

Strand: Reverse

Name: hyuA [H]

Synonym: Reut_A0045

Alternate gene names: 73539751

Gene position: 60209-58788 (Counterclockwise)

Preceding gene: 73539752

Following gene: 73539750

Centisome position: 1.58

GC content: 66.1

Gene sequence:

>1422_bases
ATGCCCGCAGACAACAACCTAGACCTGGTGATCCGCAATGCCGACGTGGTCACCGCTTCGGACCGCTTCCAGTGCGATAT
CGGCGTGCGCGACGGCCGCATCGTCGCGCTCGGCCACGGCTTGCCGCAAGGCAACCGTGAAGTCGATGCCACCGGCCTGC
TCGCGCTGCCCGGCGGCGTCGACGGCCACTGCCACCTGGACCAGCCCATGCCCGACGGCATGCGCATGGCCGATAATTTC
TTCACCGGTACGCGCGCGGCTGTGTGCGGCGGCACCACCACGGTGATCCCGTTCGCCGCGCAGGAAAAGGGCGCTTCGCT
CAAGGCCGCGGTGGCGGACTACCATCGCCGCGCCGAAGGCCGCGCCGTGATCGACTACGCGTTCCACCTGATCGTGGCCG
ACCCAACCCCCACTGTGCTGCAGGACGAACTGCCCTCGCTGATCCGCAAGGGCTATACCTCGTTCAAGGTCTACATGACC
TATGACGACCTCAAGCTGTCGGATCGCGAAATGCTGGAAGTGCTGGACGTCGCGCGCGAACACAAGGCGCTCGTGATGGT
CCACGCGGAAAACTCGGACTGCATCTCATGGCTGACCGACAAGCTGACCGGCGAAGGGCGCATCGCCCCGCGTTTCCACG
GGCTCGCGCGTCCCGCTCCGGTCGAGCGCGAGGCCACGCACCGCGCCATCACGTTCGCGGAACTCGTCGACGTGCCAATC
CTGATCGTGCATGTGTCGGGCAAGGAGGCGATCGAGCAGATTCGCTGGGCGCAGGGCAGGGGACTGAAGATCCTGGCCGA
GACCTGCCCGCAGTACCTGTACCTCACCGCGGACGACATGGGCCTGCCCGGCGACGATGGCTATGAAGGCGCCAAGTGCG
TGTGCAGCCCTCCGCCGCGCGATCCGGAGAACCAGGCCGCCGTGTGGCGTGCGCTGGTGAACGGCGTATTCAGCGTGTTC
TCGTCGGACCATGCGCCGTTCAATTACGACGATCCGGAAGGCAAGAAGCTTGGCGGTAGCACCGAGCATTCGTTCGATCA
CATTCCCAACGGCGTGCCGGGCATCGAAACGCGCCTGCCGCTGCTGTTCGACGGCGTCGCTCAGGGCAAGCTGTCGCTGC
ACCAGTTCGTCGAGCTGACCTCATACCGCCCGGCCAAGATCTACGGCCTGTATCCGCGCAAGGGCACCATTGCCGTCGGC
GCCGATGCAGACATCGTGCTGTGGGATCCGGCGCGCAAGGTGCGCATCACCAACAGCGCGCTGCACCATGCTGTCGACTA
CACGCCGTATGAAGGCCTCGACGTCACCGGCTGGCCCGTGACCTGCTTCTCGCGCGGCGACATGCTGGTGCGCGATGGCG
AGTACCTGGAGCCCGCAGCCGGACGCGGCAGGTTCCTCGAAGCCGGCCAACCCGAGGTCTGA

Upstream 100 bases:

>100_bases
CCTGGCTGGCGTCGACACGCGCGCGCTGACCGGCTGGGGTCGTCTGTTCCGCGAAGTCCAGTAAGGCAGAGTACAGAGGC
AACGAACCGGAGTCATGACC

Downstream 100 bases:

>100_bases
ACCCGAACCATCGCAAACCCGATATGAACCTCCTGATCGTCAACCCCAACATCAGCGAGTCGGTCACCGACCTGATTCAC
GCGGAAGCCCTGCGCACCGC

Product: phenylhydantoinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MPADNNLDLVIRNADVVTASDRFQCDIGVRDGRIVALGHGLPQGNREVDATGLLALPGGVDGHCHLDQPMPDGMRMADNF
FTGTRAAVCGGTTTVIPFAAQEKGASLKAAVADYHRRAEGRAVIDYAFHLIVADPTPTVLQDELPSLIRKGYTSFKVYMT
YDDLKLSDREMLEVLDVAREHKALVMVHAENSDCISWLTDKLTGEGRIAPRFHGLARPAPVEREATHRAITFAELVDVPI
LIVHVSGKEAIEQIRWAQGRGLKILAETCPQYLYLTADDMGLPGDDGYEGAKCVCSPPPRDPENQAAVWRALVNGVFSVF
SSDHAPFNYDDPEGKKLGGSTEHSFDHIPNGVPGIETRLPLLFDGVAQGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVG
ADADIVLWDPARKVRITNSALHHAVDYTPYEGLDVTGWPVTCFSRGDMLVRDGEYLEPAAGRGRFLEAGQPEV

Sequences:

>Translated_473_residues
MPADNNLDLVIRNADVVTASDRFQCDIGVRDGRIVALGHGLPQGNREVDATGLLALPGGVDGHCHLDQPMPDGMRMADNF
FTGTRAAVCGGTTTVIPFAAQEKGASLKAAVADYHRRAEGRAVIDYAFHLIVADPTPTVLQDELPSLIRKGYTSFKVYMT
YDDLKLSDREMLEVLDVAREHKALVMVHAENSDCISWLTDKLTGEGRIAPRFHGLARPAPVEREATHRAITFAELVDVPI
LIVHVSGKEAIEQIRWAQGRGLKILAETCPQYLYLTADDMGLPGDDGYEGAKCVCSPPPRDPENQAAVWRALVNGVFSVF
SSDHAPFNYDDPEGKKLGGSTEHSFDHIPNGVPGIETRLPLLFDGVAQGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVG
ADADIVLWDPARKVRITNSALHHAVDYTPYEGLDVTGWPVTCFSRGDMLVRDGEYLEPAAGRGRFLEAGQPEV
>Mature_472_residues
PADNNLDLVIRNADVVTASDRFQCDIGVRDGRIVALGHGLPQGNREVDATGLLALPGGVDGHCHLDQPMPDGMRMADNFF
TGTRAAVCGGTTTVIPFAAQEKGASLKAAVADYHRRAEGRAVIDYAFHLIVADPTPTVLQDELPSLIRKGYTSFKVYMTY
DDLKLSDREMLEVLDVAREHKALVMVHAENSDCISWLTDKLTGEGRIAPRFHGLARPAPVEREATHRAITFAELVDVPIL
IVHVSGKEAIEQIRWAQGRGLKILAETCPQYLYLTADDMGLPGDDGYEGAKCVCSPPPRDPENQAAVWRALVNGVFSVFS
SDHAPFNYDDPEGKKLGGSTEHSFDHIPNGVPGIETRLPLLFDGVAQGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGA
DADIVLWDPARKVRITNSALHHAVDYTPYEGLDVTGWPVTCFSRGDMLVRDGEYLEPAAGRGRFLEAGQPEV

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=468, Percent_Identity=38.2478632478632, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI4503051, Length=464, Percent_Identity=35.3448275862069, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI62422571, Length=464, Percent_Identity=35.3448275862069, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4503379, Length=465, Percent_Identity=35.2688172043011, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI190194363, Length=462, Percent_Identity=35.7142857142857, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI4503377, Length=465, Percent_Identity=35.2688172043011, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI19923821, Length=466, Percent_Identity=33.9055793991416, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI18105007, Length=409, Percent_Identity=25.9168704156479, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI87082175, Length=465, Percent_Identity=38.0645161290323, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1786722, Length=473, Percent_Identity=30.0211416490486, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17539558, Length=466, Percent_Identity=34.549356223176, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71989490, Length=467, Percent_Identity=33.4047109207709, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI86575075, Length=459, Percent_Identity=27.4509803921569, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=413, Percent_Identity=24.2130750605327, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322218, Length=409, Percent_Identity=30.8068459657702, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI221377917, Length=468, Percent_Identity=36.5384615384615, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17137462, Length=466, Percent_Identity=34.7639484978541, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24644287, Length=298, Percent_Identity=35.9060402684564, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24644289, Length=232, Percent_Identity=36.6379310344828, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24642586, Length=414, Percent_Identity=27.0531400966184, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859883, Length=410, Percent_Identity=27.8048780487805, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059
- InterPro:   IPR011612 [H]

Pfam domain/function: PF01979 Amidohydro_1; PF00449 Urease_alpha [H]

EC number: 3.5.2.2

Molecular weight: Translated: 51619; Mature: 51488

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADNNLDLVIRNADVVTASDRFQCDIGVRDGRIVALGHGLPQGNREVDATGLLALPGGV
CCCCCCEEEEEECCEEEEECCCEEEEEECCCCEEEEEECCCCCCCCEECCCEEEECCCCC
DGHCHLDQPMPDGMRMADNFFTGTRAAVCGGTTTVIPFAAQEKGASLKAAVADYHRRAEG
CCCEEECCCCCCCHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
RAVIDYAFHLIVADPTPTVLQDELPSLIRKGYTSFKVYMTYDDLKLSDREMLEVLDVARE
CEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHC
HKALVMVHAENSDCISWLTDKLTGEGRIAPRFHGLARPAPVEREATHRAITFAELVDVPI
CCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCE
LIVHVSGKEAIEQIRWAQGRGLKILAETCPQYLYLTADDMGLPGDDGYEGAKCVCSPPPR
EEEEECCHHHHHHHHHHCCCCEEEEHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCC
DPENQAAVWRALVNGVFSVFSSDHAPFNYDDPEGKKLGGSTEHSFDHIPNGVPGIETRLP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
LLFDGVAQGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGADADIVLWDPARKVRITNSA
CEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEECCCCEEEEEHHH
LHHAVDYTPYEGLDVTGWPVTCFSRGDMLVRDGEYLEPAAGRGRFLEAGQPEV
HHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure 
PADNNLDLVIRNADVVTASDRFQCDIGVRDGRIVALGHGLPQGNREVDATGLLALPGGV
CCCCCEEEEEECCEEEEECCCEEEEEECCCCEEEEEECCCCCCCCEECCCEEEECCCCC
DGHCHLDQPMPDGMRMADNFFTGTRAAVCGGTTTVIPFAAQEKGASLKAAVADYHRRAEG
CCCEEECCCCCCCHHHHHHHCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
RAVIDYAFHLIVADPTPTVLQDELPSLIRKGYTSFKVYMTYDDLKLSDREMLEVLDVARE
CEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHC
HKALVMVHAENSDCISWLTDKLTGEGRIAPRFHGLARPAPVEREATHRAITFAELVDVPI
CCEEEEEECCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCE
LIVHVSGKEAIEQIRWAQGRGLKILAETCPQYLYLTADDMGLPGDDGYEGAKCVCSPPPR
EEEEECCHHHHHHHHHHCCCCEEEEHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCC
DPENQAAVWRALVNGVFSVFSSDHAPFNYDDPEGKKLGGSTEHSFDHIPNGVPGIETRLP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
LLFDGVAQGKLSLHQFVELTSYRPAKIYGLYPRKGTIAVGADADIVLWDPARKVRITNSA
CEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCEEEECCCCEEEEEHHH
LHHAVDYTPYEGLDVTGWPVTCFSRGDMLVRDGEYLEPAAGRGRFLEAGQPEV
HHHHCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA