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The map label for this gene is maiA [H]

Identifier: 73539682

GI number: 73539682

Start: 2700885

End: 2701526

Strand: Reverse

Name: maiA [H]

Synonym: Reut_B5862

Alternate gene names: 73539682

Gene position: 2701526-2700885 (Counterclockwise)

Preceding gene: 73539683

Following gene: 73539681

Centisome position: 99.1

GC content: 65.89

Gene sequence:

>642_bases
ATGCAGCTCTATAGTTTCTTCAACAGCTCCACGTCCTACCGCGTGCGCATTGCACTGGCGCTCAAGGGCCTGCCGTTCGA
GACCCTGCCGGTCAACATCCGCACCGGCCAGCACCGCGAGGCGGAGTACGTGCAAGGCATCAACCCGTCTGCGTGCGTAC
CCGCGCTGGTGGACGGCGACTTCACGCTCGGACAGTCGCTTGCGATCATGGACTACCTTGACGCGCACTACCCGGAACCG
CGGCTGCTGCCGCAGGACGCGGAGCCGCGTGCGCGCGTGCTGGAACTGACCAGCCTGATCGCCTGCGACATCCATCCGGT
CAACAACCTGCGCGTACTGCGCTACCTGCAGGATGTGCTCAAGGTGACGCCGGAACAGAAGGATGCCTGGTACCGCCACT
GGGTCGATGAAGGCATGGCCGGTGTGGAACGCCTGCTCGCGCTGCACGGCAAGGGCCCGTGGTGTTTCGGTGACAAGCCG
ACCCTGGCCGACGTCACGCTGGTGCCGCAGATCGCCAACGTGCAGCGCATGGGCTGCGACCTGTCGCCCTACCCGCGCGC
CATGGCGGTCTATGCGCATGCCACGGCGCACCCCGCGTTTGCCAAAGCTGCGCCCACGCAGCAACCCGATTACACGGCCT
GA

Upstream 100 bases:

>100_bases
CGGTCAAGGCCGGCGACACCATGGTGACCGGCGTCGACGGCCTCGGCGAACTCAAGGTCCGCGTGGTCTGATATCCACGC
CGGCAAGCAGCGAAGCCATC

Downstream 100 bases:

>100_bases
TTCGCTGACCCGGCCAGGAGACAAGCATGAATACATCACAAGACACGGGCCGCAAGGTTCTGATCATCGGCGGCGGCATC
GGCGGGCTGGCAGCGGCGCT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLYSFFNSSTSYRVRIALALKGLPFETLPVNIRTGQHREAEYVQGINPSACVPALVDGDFTLGQSLAIMDYLDAHYPEP
RLLPQDAEPRARVLELTSLIACDIHPVNNLRVLRYLQDVLKVTPEQKDAWYRHWVDEGMAGVERLLALHGKGPWCFGDKP
TLADVTLVPQIANVQRMGCDLSPYPRAMAVYAHATAHPAFAKAAPTQQPDYTA

Sequences:

>Translated_213_residues
MQLYSFFNSSTSYRVRIALALKGLPFETLPVNIRTGQHREAEYVQGINPSACVPALVDGDFTLGQSLAIMDYLDAHYPEP
RLLPQDAEPRARVLELTSLIACDIHPVNNLRVLRYLQDVLKVTPEQKDAWYRHWVDEGMAGVERLLALHGKGPWCFGDKP
TLADVTLVPQIANVQRMGCDLSPYPRAMAVYAHATAHPAFAKAAPTQQPDYTA
>Mature_213_residues
MQLYSFFNSSTSYRVRIALALKGLPFETLPVNIRTGQHREAEYVQGINPSACVPALVDGDFTLGQSLAIMDYLDAHYPEP
RLLPQDAEPRARVLELTSLIACDIHPVNNLRVLRYLQDVLKVTPEQKDAWYRHWVDEGMAGVERLLALHGKGPWCFGDKP
TLADVTLVPQIANVQRMGCDLSPYPRAMAVYAHATAHPAFAKAAPTQQPDYTA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=41.6267942583732, Blast_Score=161, Evalue=3e-40,
Organism=Homo sapiens, GI22202622, Length=158, Percent_Identity=38.6075949367089, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI4504187, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI124249394, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1789624, Length=182, Percent_Identity=27.4725274725275, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17551302, Length=213, Percent_Identity=36.6197183098592, Blast_Score=126, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=34.9282296650718, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=36.3636363636364, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645375, Length=209, Percent_Identity=39.7129186602871, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355859, Length=209, Percent_Identity=39.7129186602871, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553325, Length=209, Percent_Identity=39.7129186602871, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=35.7142857142857, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23727; Mature: 23727

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLPFETLPVNIRTGQHREAEYVQGINPSACVPALVDGD
CCCEEECCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEECCC
FTLGQSLAIMDYLDAHYPEPRLLPQDAEPRARVLELTSLIACDIHPVNNLRVLRYLQDVL
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KVTPEQKDAWYRHWVDEGMAGVERLLALHGKGPWCFGDKPTLADVTLVPQIANVQRMGCD
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCC
LSPYPRAMAVYAHATAHPAFAKAAPTQQPDYTA
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLPFETLPVNIRTGQHREAEYVQGINPSACVPALVDGD
CCCEEECCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEECCC
FTLGQSLAIMDYLDAHYPEPRLLPQDAEPRARVLELTSLIACDIHPVNNLRVLRYLQDVL
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KVTPEQKDAWYRHWVDEGMAGVERLLALHGKGPWCFGDKPTLADVTLVPQIANVQRMGCD
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCC
LSPYPRAMAVYAHATAHPAFAKAAPTQQPDYTA
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]