Click here to switch to the map view.

The map label for this gene is pcaK [H]

Identifier: 73539680

GI number: 73539680

Start: 2698200

End: 2699543

Strand: Reverse

Name: pcaK [H]

Synonym: Reut_B5860

Alternate gene names: 73539680

Gene position: 2699543-2698200 (Counterclockwise)

Preceding gene: 73539681

Following gene: 73539675

Centisome position: 99.02

GC content: 67.26

Gene sequence:

>1344_bases
ATGCCTGCCAGCCAGACCATCAATATTTCCGCGTTCATCGACCGCCAGCCGCTGTCGCCCTTCCAGGTCATGATCGTGGT
GCTGTGCTTTCTGATCGTCGCCATCGATGGCTTCGATACCGCCGCCATCGGCTTCATCGCGCCGGCGATCCGCGCCGAAT
GGCAACTGACGCCGGCGCACCTCGCACCGCTGTTCGGTGCCGGACTCGGCGGCCTGATGGCCGGCGCTTTCCTGTTCGGC
CCGCTTGCCGACCGCTTCGGCCGCAAGAGCGTGCTGGTGTTCTCCGTGCTGTTCTTCGGCGTGGCAAGCCTCGCTTCCGC
GTGGTCGGGCGGGTTGTGGGAGCTGATTGCGCTGCGCTTCCTGACTGGCCTTGGACTCGGCGGAGCCATGCCCAACACCA
TCACGCTGACGTCCGAATTCTGTCCGGAGAAGCGCCGCTCGTTCCTGGTCACGACCATGTTCTGCGGCTTCACGCTCGGC
TCGGCGCTCGGTGGCCTCGCTTCGGCGGGATTGATCGAGGCGTATGGCTGGCGTTCAGTGCTCGTCGTTGGCGGCGTGCT
GCCGCTGGTGCTGTCGGTGCTCCTGGCCCTGCTGCTGCCGGAATCGGTGCGCTACCTGGTGATGGCCCGCCGCGCGCCGG
AACGCATCGCCGCAACGCTGCGGCGCATTGCCCCGCAGGCCAACCTGGATGGCGCCACCTTCACGGTGTCGGAATCCCGC
AGCACCAGCTCGCCGGTGCGCCACCTGTTCAAGCCCGAGCTTGTACGCGGCACGCTGCTGTTCTGGCTGACCTTCTTCAT
GAGCCTGCTGGTGATCTACCTGCTGTCGAGCTGGCTGCCGACGCTGCTGCGCGGCACCGGCCTGTCGCTGCGCACCGCGG
CGCTGGTCACCACCATGTTCCAGGTCGGCGGCACGCTTGGCGCCATCGTGCTGGGCTGGCTGATGGACCGATTCAATCCG
CACTACGTGCTGGCGATCAGCTACGCGCTGGCCGGCGTGTTCGTCGCCGCGATCGGCAGCCTGGCCGATACTCCGATGCT
GGCAGGTGTCGCGGTATTCCTCGCTGGCTTCTGCGTCTCGGGTTCGCAGGTCGGCGCCAACGCATTGTCGGCCGGCTTCT
ATCCGACCGACTGCCGCGCGACCGGCGTGAGCTGGGCCAATGGCGTAGGCCGGATGGGCTCGGTGGTGGGCTCTGTCGGC
GGTGCGACGATGCTGTCGATGGATCTGGGCATGCCCACGCTGTTCTTGCTGATCGGCATCCCCGCTCTGCTGGCAGGGGG
CACCATGCTGTCGCTTGGGCTGGCACGCCGCGCGCCTGTACGGCAAGAGCTGGCCGCGAGCTGA

Upstream 100 bases:

>100_bases
CACCCCGCAAACCCAAGCAGTAACCGACAAGGTGACTGGCGGCCCATCCGGCCGCCGGCCCGAGTTCGTCCCGAAACAGG
CAACGAGGAGGAGACAACCC

Downstream 100 bases:

>100_bases
CGGCCGGCAGCGTCAGTGAGCCCGCAGCGCATCGACGATGTTCATGCGGGCCGCGCGCAATGCCGGCAGGAAGCCGCCGA
TCAGCCCCATCGCCATCGAG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPASQTINISAFIDRQPLSPFQVMIVVLCFLIVAIDGFDTAAIGFIAPAIRAEWQLTPAHLAPLFGAGLGGLMAGAFLFG
PLADRFGRKSVLVFSVLFFGVASLASAWSGGLWELIALRFLTGLGLGGAMPNTITLTSEFCPEKRRSFLVTTMFCGFTLG
SALGGLASAGLIEAYGWRSVLVVGGVLPLVLSVLLALLLPESVRYLVMARRAPERIAATLRRIAPQANLDGATFTVSESR
STSSPVRHLFKPELVRGTLLFWLTFFMSLLVIYLLSSWLPTLLRGTGLSLRTAALVTTMFQVGGTLGAIVLGWLMDRFNP
HYVLAISYALAGVFVAAIGSLADTPMLAGVAVFLAGFCVSGSQVGANALSAGFYPTDCRATGVSWANGVGRMGSVVGSVG
GATMLSMDLGMPTLFLLIGIPALLAGGTMLSLGLARRAPVRQELAAS

Sequences:

>Translated_447_residues
MPASQTINISAFIDRQPLSPFQVMIVVLCFLIVAIDGFDTAAIGFIAPAIRAEWQLTPAHLAPLFGAGLGGLMAGAFLFG
PLADRFGRKSVLVFSVLFFGVASLASAWSGGLWELIALRFLTGLGLGGAMPNTITLTSEFCPEKRRSFLVTTMFCGFTLG
SALGGLASAGLIEAYGWRSVLVVGGVLPLVLSVLLALLLPESVRYLVMARRAPERIAATLRRIAPQANLDGATFTVSESR
STSSPVRHLFKPELVRGTLLFWLTFFMSLLVIYLLSSWLPTLLRGTGLSLRTAALVTTMFQVGGTLGAIVLGWLMDRFNP
HYVLAISYALAGVFVAAIGSLADTPMLAGVAVFLAGFCVSGSQVGANALSAGFYPTDCRATGVSWANGVGRMGSVVGSVG
GATMLSMDLGMPTLFLLIGIPALLAGGTMLSLGLARRAPVRQELAAS
>Mature_446_residues
PASQTINISAFIDRQPLSPFQVMIVVLCFLIVAIDGFDTAAIGFIAPAIRAEWQLTPAHLAPLFGAGLGGLMAGAFLFGP
LADRFGRKSVLVFSVLFFGVASLASAWSGGLWELIALRFLTGLGLGGAMPNTITLTSEFCPEKRRSFLVTTMFCGFTLGS
ALGGLASAGLIEAYGWRSVLVVGGVLPLVLSVLLALLLPESVRYLVMARRAPERIAATLRRIAPQANLDGATFTVSESRS
TSSPVRHLFKPELVRGTLLFWLTFFMSLLVIYLLSSWLPTLLRGTGLSLRTAALVTTMFQVGGTLGAIVLGWLMDRFNPH
YVLAISYALAGVFVAAIGSLADTPMLAGVAVFLAGFCVSGSQVGANALSAGFYPTDCRATGVSWANGVGRMGSVVGSVGG
ATMLSMDLGMPTLFLLIGIPALLAGGTMLSLGLARRAPVRQELAAS

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=432, Percent_Identity=26.3888888888889, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI166064021, Length=339, Percent_Identity=26.5486725663717, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI8923870, Length=361, Percent_Identity=26.8698060941828, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI213021148, Length=236, Percent_Identity=29.6610169491525, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI296080734, Length=397, Percent_Identity=26.448362720403, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI296080721, Length=397, Percent_Identity=26.1964735516373, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI296080719, Length=397, Percent_Identity=26.1964735516373, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI24497499, Length=397, Percent_Identity=26.1964735516373, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4507005, Length=382, Percent_Identity=27.2251308900524, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI7662270, Length=206, Percent_Identity=26.2135922330097, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87081723, Length=419, Percent_Identity=34.8448687350835, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082159, Length=393, Percent_Identity=27.9898218829517, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788068, Length=460, Percent_Identity=23.9130434782609, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082231, Length=411, Percent_Identity=25.3041362530414, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786229, Length=355, Percent_Identity=25.6338028169014, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=203, Percent_Identity=27.0935960591133, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788074, Length=360, Percent_Identity=23.0555555555556, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789515, Length=315, Percent_Identity=22.8571428571429, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789152, Length=305, Percent_Identity=23.2786885245902, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=437, Percent_Identity=24.9427917620137, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922874, Length=392, Percent_Identity=25.5102040816327, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640198, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640196, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640200, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI161077756, Length=440, Percent_Identity=24.7727272727273, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46889; Mature: 46758

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00318 HMG_COA_REDUCTASE_2 ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASQTINISAFIDRQPLSPFQVMIVVLCFLIVAIDGFDTAAIGFIAPAIRAEWQLTPAH
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
LAPLFGAGLGGLMAGAFLFGPLADRFGRKSVLVFSVLFFGVASLASAWSGGLWELIALRF
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LTGLGLGGAMPNTITLTSEFCPEKRRSFLVTTMFCGFTLGSALGGLASAGLIEAYGWRSV
HHHCCCCCCCCCCEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVVGGVLPLVLSVLLALLLPESVRYLVMARRAPERIAATLRRIAPQANLDGATFTVSESR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCC
STSSPVRHLFKPELVRGTLLFWLTFFMSLLVIYLLSSWLPTLLRGTGLSLRTAALVTTMF
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QVGGTLGAIVLGWLMDRFNPHYVLAISYALAGVFVAAIGSLADTPMLAGVAVFLAGFCVS
HHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GSQVGANALSAGFYPTDCRATGVSWANGVGRMGSVVGSVGGATMLSMDLGMPTLFLLIGI
CCHHCCHHHHCCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCHHHEEHHCCHHHHHHHHHH
PALLAGGTMLSLGLARRAPVRQELAAS
HHHHHCCHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
PASQTINISAFIDRQPLSPFQVMIVVLCFLIVAIDGFDTAAIGFIAPAIRAEWQLTPAH
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
LAPLFGAGLGGLMAGAFLFGPLADRFGRKSVLVFSVLFFGVASLASAWSGGLWELIALRF
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LTGLGLGGAMPNTITLTSEFCPEKRRSFLVTTMFCGFTLGSALGGLASAGLIEAYGWRSV
HHHCCCCCCCCCCEEECHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LVVGGVLPLVLSVLLALLLPESVRYLVMARRAPERIAATLRRIAPQANLDGATFTVSESR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCC
STSSPVRHLFKPELVRGTLLFWLTFFMSLLVIYLLSSWLPTLLRGTGLSLRTAALVTTMF
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QVGGTLGAIVLGWLMDRFNPHYVLAISYALAGVFVAAIGSLADTPMLAGVAVFLAGFCVS
HHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GSQVGANALSAGFYPTDCRATGVSWANGVGRMGSVVGSVGGATMLSMDLGMPTLFLLIGI
CCHHCCHHHHCCCCCCCCCCCCCHHHHCHHHHHHHHHHCCCHHHEEHHCCHHHHHHHHHH
PALLAGGTMLSLGLARRAPVRQELAAS
HHHHHCCHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]