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The map label for this gene is macA [H]

Identifier: 73539676

GI number: 73539676

Start: 2693868

End: 2695145

Strand: Direct

Name: macA [H]

Synonym: Reut_B5856

Alternate gene names: 73539676

Gene position: 2693868-2695145 (Clockwise)

Preceding gene: 73539665

Following gene: 73539677

Centisome position: 98.82

GC content: 65.1

Gene sequence:

>1278_bases
TTGTCCCCGCGACAACGAGCCCAAAGTGCCCCCAGCGCTATACTGACAACCTCTCCCAGCAGGATGACCCGATTGGCCGA
CCAAGACCTCTCCCGGCTGAAAATCGACCGCGGTCCACACGCGGCCGCGCCGCGCCGCCGCCGGTGGCTGCTTTATGTGG
CCATCGCCGTGGCGCTCGCTGCAGCTGCTGCTATCGCCTTCAAGGTAGCCAGTCCCGCCGCGGTGGAAACCGTCACGGTC
TCGTCCGCCTATCCTTCCCAAAACTACACCCTGCTTAACGCTACCGGCTATGTCGTGCCGCAACGCAAGGCGGCTGTCGC
CTCCAAGGCGCAAGGCAGGCTGGAGTGGCTGGGCGTGCTGGAAGGTTCGCGCGTAAAGAAGGACGAAATCATCGCCCGGA
TAGAACGCCGGGACGTCGATGCCACGCTTGCGCAGGCGACCGCGCAGGTGAAGGTAGCGCAAGCCAATCTGGAATTGCAG
CTCGCCGAGCTGCGCGACGCGGAAGTGAACCTGCGCCGCTCGGAACAACTGCTGGGCCCGAAGGCGATTTCGGTCCAGCA
GTATGATGCCGACCTGGCCCGCTTCAAGAAGGCGCAGGCGTCGGTCTCGAATTCGCGTGCGGCCATCACCTCCGCGCAGG
CGAATGCGCAGGCTGCGCAAGTGTCGGTCGACCAGACGGTCATTCGCGCGCCGTTCGACGGAGTGGTGCTGATCAAGCAT
GCCAACGTGGGCGACAACATCACGCCGTTCTCGTCGGCGTCCGACACCAAGGGCGCGGTCGTGACGATTGCGGACATGGA
TACGCTCGAAGTCGAGGCCGACGTCGCTGAATCCAATATCGCGAAGATCCGCGCGGACCAGCCGTGTCTGCTGCAGCTTG
ATGCGCTGCCCGATATGCGATTCGCGGGCCAAGTGTCGCGCATCGTGCCGACCGTGGACCGCTCCAAGGCGACCGTGCTG
GTCAAGGTGCGCTTTGTCGACCGCGACGCGCGCGTGCTGCCCGACATGAGCGCCAAGATTGCGTTCCTGTCCAAACCGCT
GGCCAATCAGGACCGCAAGGCCGTTATCGCCGTGCAGCCGGCCGCGATTGCCTCCCGTGACGGCCACAAGGTGGTGTTCG
TCGTCCGCGACGGCAAGGCGCATGAAACTACCGTGACGACCGGCGAGCGCATCGGCGAACTGGTGGCCGTTCACGGCGTG
GCCGCGGGCGATGTGCTGGTGCTTTCGCCCGGTGACAAGCTGCACGACGGCGCCCGCGTCACCGTGGCGAAGAAGTAA

Upstream 100 bases:

>100_bases
GTCTGCATTGTTCATCTCCTTTGCAAACGTTTGCAGCAAACGTTTGCAATTATGATGGGTATAAGGAAATGGTCAAGGGA
ATTTTTTAGGTGGCGTGCGG

Downstream 100 bases:

>100_bases
CGTGGCCGCGATCCCGCTCGTCAGCATCCGCCACGTCGCCAAGTCATACCGGCGCGGCATGCAGACCGTGCCCGTGCTGA
CCGATATCACGCTCGACATT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSPRQRAQSAPSAILTTSPSRMTRLADQDLSRLKIDRGPHAAAPRRRRWLLYVAIAVALAAAAAIAFKVASPAAVETVTV
SSAYPSQNYTLLNATGYVVPQRKAAVASKAQGRLEWLGVLEGSRVKKDEIIARIERRDVDATLAQATAQVKVAQANLELQ
LAELRDAEVNLRRSEQLLGPKAISVQQYDADLARFKKAQASVSNSRAAITSAQANAQAAQVSVDQTVIRAPFDGVVLIKH
ANVGDNITPFSSASDTKGAVVTIADMDTLEVEADVAESNIAKIRADQPCLLQLDALPDMRFAGQVSRIVPTVDRSKATVL
VKVRFVDRDARVLPDMSAKIAFLSKPLANQDRKAVIAVQPAAIASRDGHKVVFVVRDGKAHETTVTTGERIGELVAVHGV
AAGDVLVLSPGDKLHDGARVTVAKK

Sequences:

>Translated_425_residues
MSPRQRAQSAPSAILTTSPSRMTRLADQDLSRLKIDRGPHAAAPRRRRWLLYVAIAVALAAAAAIAFKVASPAAVETVTV
SSAYPSQNYTLLNATGYVVPQRKAAVASKAQGRLEWLGVLEGSRVKKDEIIARIERRDVDATLAQATAQVKVAQANLELQ
LAELRDAEVNLRRSEQLLGPKAISVQQYDADLARFKKAQASVSNSRAAITSAQANAQAAQVSVDQTVIRAPFDGVVLIKH
ANVGDNITPFSSASDTKGAVVTIADMDTLEVEADVAESNIAKIRADQPCLLQLDALPDMRFAGQVSRIVPTVDRSKATVL
VKVRFVDRDARVLPDMSAKIAFLSKPLANQDRKAVIAVQPAAIASRDGHKVVFVVRDGKAHETTVTTGERIGELVAVHGV
AAGDVLVLSPGDKLHDGARVTVAKK
>Mature_424_residues
SPRQRAQSAPSAILTTSPSRMTRLADQDLSRLKIDRGPHAAAPRRRRWLLYVAIAVALAAAAAIAFKVASPAAVETVTVS
SAYPSQNYTLLNATGYVVPQRKAAVASKAQGRLEWLGVLEGSRVKKDEIIARIERRDVDATLAQATAQVKVAQANLELQL
AELRDAEVNLRRSEQLLGPKAISVQQYDADLARFKKAQASVSNSRAAITSAQANAQAAQVSVDQTVIRAPFDGVVLIKHA
NVGDNITPFSSASDTKGAVVTIADMDTLEVEADVAESNIAKIRADQPCLLQLDALPDMRFAGQVSRIVPTVDRSKATVLV
KVRFVDRDARVLPDMSAKIAFLSKPLANQDRKAVIAVQPAAIASRDGHKVVFVVRDGKAHETTVTTGERIGELVAVHGVA
AGDVLVLSPGDKLHDGARVTVAKK

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=338, Percent_Identity=27.810650887574, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87081796, Length=333, Percent_Identity=27.6276276276276, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=22.0779220779221, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45381; Mature: 45250

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRQRAQSAPSAILTTSPSRMTRLADQDLSRLKIDRGPHAAAPRRRRWLLYVAIAVALA
CCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHH
AAAAIAFKVASPAAVETVTVSSAYPSQNYTLLNATGYVVPQRKAAVASKAQGRLEWLGVL
HHHHHHHEECCCCEEEEEEEECCCCCCCEEEEEECEEEECCHHHHHHHHCCCCEEEEEEC
EGSRVKKDEIIARIERRDVDATLAQATAQVKVAQANLELQLAELRDAEVNLRRSEQLLGP
CCCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCHHHHHHCCC
KAISVQQYDADLARFKKAQASVSNSRAAITSAQANAQAAQVSVDQTVIRAPFDGVVLIKH
CEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEHHHHEEECCCCCEEEEEE
ANVGDNITPFSSASDTKGAVVTIADMDTLEVEADVAESNIAKIRADQPCLLQLDALPDMR
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCH
FAGQVSRIVPTVDRSKATVLVKVRFVDRDARVLPDMSAKIAFLSKPLANQDRKAVIAVQP
HHCCHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEHHHCCCCCCCCCEEEEEECC
AAIASRDGHKVVFVVRDGKAHETTVTTGERIGELVAVHGVAAGDVLVLSPGDKLHDGARV
HHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHEEEEECCCCCCEEEECCCCCCCCCCEE
TVAKK
EEECC
>Mature Secondary Structure 
SPRQRAQSAPSAILTTSPSRMTRLADQDLSRLKIDRGPHAAAPRRRRWLLYVAIAVALA
CCHHHHHCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHH
AAAAIAFKVASPAAVETVTVSSAYPSQNYTLLNATGYVVPQRKAAVASKAQGRLEWLGVL
HHHHHHHEECCCCEEEEEEEECCCCCCCEEEEEECEEEECCHHHHHHHHCCCCEEEEEEC
EGSRVKKDEIIARIERRDVDATLAQATAQVKVAQANLELQLAELRDAEVNLRRSEQLLGP
CCCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCHHHHHHCCC
KAISVQQYDADLARFKKAQASVSNSRAAITSAQANAQAAQVSVDQTVIRAPFDGVVLIKH
CEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEHHHHEEECCCCCEEEEEE
ANVGDNITPFSSASDTKGAVVTIADMDTLEVEADVAESNIAKIRADQPCLLQLDALPDMR
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCH
FAGQVSRIVPTVDRSKATVLVKVRFVDRDARVLPDMSAKIAFLSKPLANQDRKAVIAVQP
HHCCHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEHHHCCCCCCCCCEEEEEECC
AAIASRDGHKVVFVVRDGKAHETTVTTGERIGELVAVHGVAAGDVLVLSPGDKLHDGARV
HHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHEEEEECCCCCCEEEECCCCCCCCCCEE
TVAKK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]