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The map label for this gene is catB [H]

Identifier: 73539613

GI number: 73539613

Start: 2617475

End: 2618647

Strand: Direct

Name: catB [H]

Synonym: Reut_B5793

Alternate gene names: 73539613

Gene position: 2617475-2618647 (Clockwise)

Preceding gene: 73539612

Following gene: 73539614

Centisome position: 96.01

GC content: 63.94

Gene sequence:

>1173_bases
ATGAAAATCACCAAAGTCGAATGCATTCCGGTATCGATGCCGCTGAAGAAGCCGCTGCGATTGCCGAATGTCACCATTAC
CAAGCTTGATACGCTGCTGCTGCGGCTGCATACCGAAACGGGCCTGGTGGGCATCGCCGAATCCGGCGATACGTCTTCGT
GGTACCGCGGCGAAAGCCAGGCCTCGATGGTCAGCATGATCTGCGACATATTTGCGCCGCGCATTCTTCTCGGTGAAGAC
CCGTGCAACATTGAAAAAATTGTCGGCGCAATGGACACGTTGGCGCGTGACAACAACCAGGCCAAGGGTCTCGTCGACTG
CGCGCTGCACGACCTGAAGGGCAAGGTACTGAACGTGCCGGTCTACCAGTTGCTCGGCGGACGCACACGCGAATCGGCGC
CGCTTGGCTGGGTGCTGTCGGCCGGCGAGCCGGACAAAGTGGCCGAGGCCGCGCTATCGGCCCGGGAAGCGGGCTTTGTC
GCCTTCAAGCTGAAGGTGGGGCGCGGCACCTTTGACAACGATGTGGCGACCGCCGCAGCCGTGCGCAAGGCGCTGGGCGA
TAGCGCGCGCATCTGCCTGGACGCCAATGGCTCATGGAACTACGAGCAGGCCCTGCGCACGATCCGACGGCTCGATGCGT
ATCGACTCGACTTCGTCGAGCAACCCCTGCCGCACTGGGACATCGAAGGCATGGCGCGCCTGCGACAGCGCATCGACACG
CCCGTGTACGCCGACGAGGCAGCACAGGAGCTGCATCACCTGAAGGAAATCGCCGAGCGGCGCGCCGCCGACGGCGTCAT
CCTCAAAGTGCAGAAGGTCGGTGGCCTGTTGAAGGCCCAGCGCTTCCTCGCCGTGGCCCAGATGTTCGACTTGCCAGTGA
TGTGCAGCACGCTGATCGGCTCTGGCGTGGAGGCCTGTCCGACCGCGCATCTGTGGGTGGCGAACCAGTGGGCTTGCCAG
TTCATCAGCGAGAGTACGGGGCCGCTGGCTGTGCATGGCGTCTGGAACAGCGAAGACATCCCGCAGGGCGCGGACCTTGC
CCTGCAAGGCGCGCGATTCCATCGCGGCCATGTATCCCCCGGCGACGGTCCGGGCCTTGGCGTGGAACTGAACGAGGCAC
TGCTCGGGCAGATTCGCACCGAGGGCCGTCCTGCGCGGGTGGTCAACCTGTAG

Upstream 100 bases:

>100_bases
GCAAGACCGCTGAGAATCTACGACTACGGCCCGCCGCTGGCCGCGGGCGTGGTTGCCGTGGTTGCGCTGCTCCGTGACGC
CGGAAGGAAGTGAGGCCGCC

Downstream 100 bases:

>100_bases
GGCGCCCCCGACCCCGCCAGGATTTCAATTCGTGTTGATCTAGGAGACAGACATGAGCAGGGCCAATCAATCACCGTGGA
AGTACGCGATCCTGTGGTTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKITKVECIPVSMPLKKPLRLPNVTITKLDTLLLRLHTETGLVGIAESGDTSSWYRGESQASMVSMICDIFAPRILLGED
PCNIEKIVGAMDTLARDNNQAKGLVDCALHDLKGKVLNVPVYQLLGGRTRESAPLGWVLSAGEPDKVAEAALSAREAGFV
AFKLKVGRGTFDNDVATAAAVRKALGDSARICLDANGSWNYEQALRTIRRLDAYRLDFVEQPLPHWDIEGMARLRQRIDT
PVYADEAAQELHHLKEIAERRAADGVILKVQKVGGLLKAQRFLAVAQMFDLPVMCSTLIGSGVEACPTAHLWVANQWACQ
FISESTGPLAVHGVWNSEDIPQGADLALQGARFHRGHVSPGDGPGLGVELNEALLGQIRTEGRPARVVNL

Sequences:

>Translated_390_residues
MKITKVECIPVSMPLKKPLRLPNVTITKLDTLLLRLHTETGLVGIAESGDTSSWYRGESQASMVSMICDIFAPRILLGED
PCNIEKIVGAMDTLARDNNQAKGLVDCALHDLKGKVLNVPVYQLLGGRTRESAPLGWVLSAGEPDKVAEAALSAREAGFV
AFKLKVGRGTFDNDVATAAAVRKALGDSARICLDANGSWNYEQALRTIRRLDAYRLDFVEQPLPHWDIEGMARLRQRIDT
PVYADEAAQELHHLKEIAERRAADGVILKVQKVGGLLKAQRFLAVAQMFDLPVMCSTLIGSGVEACPTAHLWVANQWACQ
FISESTGPLAVHGVWNSEDIPQGADLALQGARFHRGHVSPGDGPGLGVELNEALLGQIRTEGRPARVVNL
>Mature_390_residues
MKITKVECIPVSMPLKKPLRLPNVTITKLDTLLLRLHTETGLVGIAESGDTSSWYRGESQASMVSMICDIFAPRILLGED
PCNIEKIVGAMDTLARDNNQAKGLVDCALHDLKGKVLNVPVYQLLGGRTRESAPLGWVLSAGEPDKVAEAALSAREAGFV
AFKLKVGRGTFDNDVATAAAVRKALGDSARICLDANGSWNYEQALRTIRRLDAYRLDFVEQPLPHWDIEGMARLRQRIDT
PVYADEAAQELHHLKEIAERRAADGVILKVQKVGGLLKAQRFLAVAQMFDLPVMCSTLIGSGVEACPTAHLWVANQWACQ
FISESTGPLAVHGVWNSEDIPQGADLALQGARFHRGHVSPGDGPGLGVELNEALLGQIRTEGRPARVVNL

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=377, Percent_Identity=25.7294429708223, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081876, Length=267, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 42346; Mature: 42346

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKVECIPVSMPLKKPLRLPNVTITKLDTLLLRLHTETGLVGIAESGDTSSWYRGESQ
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHCCCHH
ASMVSMICDIFAPRILLGEDPCNIEKIVGAMDTLARDNNQAKGLVDCALHDLKGKVLNVP
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCC
VYQLLGGRTRESAPLGWVLSAGEPDKVAEAALSAREAGFVAFKLKVGRGTFDNDVATAAA
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHH
VRKALGDSARICLDANGSWNYEQALRTIRRLDAYRLDFVEQPLPHWDIEGMARLRQRIDT
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PVYADEAAQELHHLKEIAERRAADGVILKVQKVGGLLKAQRFLAVAQMFDLPVMCSTLIG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHC
SGVEACPTAHLWVANQWACQFISESTGPLAVHGVWNSEDIPQGADLALQGARFHRGHVSP
CCCCCCCCCEEEECHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCEEECCCCCC
GDGPGLGVELNEALLGQIRTEGRPARVVNL
CCCCCCCEEHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKITKVECIPVSMPLKKPLRLPNVTITKLDTLLLRLHTETGLVGIAESGDTSSWYRGESQ
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCCCCCHHHCCCHH
ASMVSMICDIFAPRILLGEDPCNIEKIVGAMDTLARDNNQAKGLVDCALHDLKGKVLNVP
HHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCC
VYQLLGGRTRESAPLGWVLSAGEPDKVAEAALSAREAGFVAFKLKVGRGTFDNDVATAAA
HHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHH
VRKALGDSARICLDANGSWNYEQALRTIRRLDAYRLDFVEQPLPHWDIEGMARLRQRIDT
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PVYADEAAQELHHLKEIAERRAADGVILKVQKVGGLLKAQRFLAVAQMFDLPVMCSTLIG
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHC
SGVEACPTAHLWVANQWACQFISESTGPLAVHGVWNSEDIPQGADLALQGARFHRGHVSP
CCCCCCCCCEEEECHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCEEECCCCCC
GDGPGLGVELNEALLGQIRTEGRPARVVNL
CCCCCCCEEHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990288 [H]