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The map label for this gene is tmoA [H]

Identifier: 73539501

GI number: 73539501

Start: 2510823

End: 2512328

Strand: Reverse

Name: tmoA [H]

Synonym: Reut_B5679

Alternate gene names: 73539501

Gene position: 2512328-2510823 (Counterclockwise)

Preceding gene: 73539516

Following gene: 73539500

Centisome position: 92.16

GC content: 64.14

Gene sequence:

>1506_bases
ATGGCGCTTTTGGAACGCGCAGCGTGGTACGACATTGCCCGCACAACGAACTGGACGCCGACGTACGTCGCCGAGTCCGA
CCTTTTCCCGGAGGTCATGGCAGGGGCGCAGGGCGTGCCCATGGAAATCTGGGAAACCTATGACGAGCCCTACAAGACGT
CGTATCCGGAGTACGTCCGCATTCAGCGCGAGAAGGACGCCGGCGCCTATTCCGTGAAGGCCGCGCTCGAGCGCAGCCGC
ATGTTCGAGGACGCCGATCCCGGCTGGCTGTCGATCCTGAAGGCGCATTACGGCGCCATCGCGCTCGGCGAATACGCGGC
GATGAGCGCGGAGGCGCGCATGGCCCGCTTCGGCCGCGCGCCAGGGATGCGCAACATGGCCACCTTCGGCATGCTCGACG
AGAACCGTCACGGGCAATTGCAGCTCTACTTTCCGCACGACTACTGCCCGAAGGACCGGCAATTTGACTGGGCGCACAAG
GCCTATCACACCAACCAATGGGGCGCGATCGCCGCCCGCAGCACCTTCGACGACCTGTTCATGTCGCGAAGCGCGATCGA
GATCGCCGTGATGCTTACGTTTGCCTTCGAAACGGGTTTCACCAACATGCAGTTCCTCGGGCTGGCGGCCGATGCGGCGG
AAGCCGGCGATTTCACCTTCGCCAGCCTCATCTCGAGCATCCAGACGGACGAGTCGCGACACGCGCAGATCGGCGGCCCG
GCACTGCAGATCCTGATCGCCAACGGGCGCAAGGAGCAGGCGCAGCAGCTCGTGGACGTCGCCATTGCACGCGCATGGCG
CCTGTTCTCCCTGCTGACTGGCACGTCGATGGACTACGCCACGCCGCTGCAGCATCGCAAGGAGTCATTCAAGGAGTTCA
TGACGGAATGGATCGTCGGCCAGTTCGAGCGCACGCTGATCGACCTCGGTCTCGACCTGCCCTGGTACTGGGACCAGATG
ATCAATGAGTTCGACTACCAGCACCATGCCTATCAGATGGGCATCTGGTTCTGGCGGCCGACCGTCTGGTGGAACCCGGC
CGCCGGCATGACGCCGGACTGCCGTGACTGGCTCGAGGAAAAGTATCCCGGCTGGAACGACACGTTCGGCAAGGCCTGGG
ACGTCATCATCGACAACCTGCTTGCGGGCAGGAAGGAATTGACCGTCCCCGAGACCCTGCCCATCGTCTGCAACATGAGC
CAGTTGCCAATCTGCGCGGTGCCCGGCAACGGCTGGAACGTCAGGGACTATCCGCTCGAGTACAACGGCCGCACGTACCA
CTTCAACTCCGAGATCGACCGCTGGGTCTTCCAGCAGGACCCGGTGCGCTACCGCGACCACCTGACCCTGGTCGACCGTT
TCCTCGCCGGGCACATCCAGCCGCCCGACCTGGGGGGCGCACTCCGGTACATGAACCTCGCCCCCGGCGAGATTGGCGAC
GACGCACACCAGTACGCGTGGGTAGAGGCCTACCGCCGCCAGCGCGAACAGAACAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCATTGCATTTGCGAAAGGGACAGCGACCCCGGGATCGCGGCCTTTCGCAACCGGGGCAGCAAAGCAAGAAAGACATGAC
ATTCACCAAGGAGATATCCC

Downstream 100 bases:

>100_bases
CGCGGCCATCAGATGGAGGATGACATGGCACTGTTTCCAGTGATTTCGAATTTTCAGTACGACTTCGTGCTGCAGCTCGT
CGCCGTCGATACGGAGAACT

Product: methane/phenol/toluene hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MALLERAAWYDIARTTNWTPTYVAESDLFPEVMAGAQGVPMEIWETYDEPYKTSYPEYVRIQREKDAGAYSVKAALERSR
MFEDADPGWLSILKAHYGAIALGEYAAMSAEARMARFGRAPGMRNMATFGMLDENRHGQLQLYFPHDYCPKDRQFDWAHK
AYHTNQWGAIAARSTFDDLFMSRSAIEIAVMLTFAFETGFTNMQFLGLAADAAEAGDFTFASLISSIQTDESRHAQIGGP
ALQILIANGRKEQAQQLVDVAIARAWRLFSLLTGTSMDYATPLQHRKESFKEFMTEWIVGQFERTLIDLGLDLPWYWDQM
INEFDYQHHAYQMGIWFWRPTVWWNPAAGMTPDCRDWLEEKYPGWNDTFGKAWDVIIDNLLAGRKELTVPETLPIVCNMS
QLPICAVPGNGWNVRDYPLEYNGRTYHFNSEIDRWVFQQDPVRYRDHLTLVDRFLAGHIQPPDLGGALRYMNLAPGEIGD
DAHQYAWVEAYRRQREQNKAA

Sequences:

>Translated_501_residues
MALLERAAWYDIARTTNWTPTYVAESDLFPEVMAGAQGVPMEIWETYDEPYKTSYPEYVRIQREKDAGAYSVKAALERSR
MFEDADPGWLSILKAHYGAIALGEYAAMSAEARMARFGRAPGMRNMATFGMLDENRHGQLQLYFPHDYCPKDRQFDWAHK
AYHTNQWGAIAARSTFDDLFMSRSAIEIAVMLTFAFETGFTNMQFLGLAADAAEAGDFTFASLISSIQTDESRHAQIGGP
ALQILIANGRKEQAQQLVDVAIARAWRLFSLLTGTSMDYATPLQHRKESFKEFMTEWIVGQFERTLIDLGLDLPWYWDQM
INEFDYQHHAYQMGIWFWRPTVWWNPAAGMTPDCRDWLEEKYPGWNDTFGKAWDVIIDNLLAGRKELTVPETLPIVCNMS
QLPICAVPGNGWNVRDYPLEYNGRTYHFNSEIDRWVFQQDPVRYRDHLTLVDRFLAGHIQPPDLGGALRYMNLAPGEIGD
DAHQYAWVEAYRRQREQNKAA
>Mature_500_residues
ALLERAAWYDIARTTNWTPTYVAESDLFPEVMAGAQGVPMEIWETYDEPYKTSYPEYVRIQREKDAGAYSVKAALERSRM
FEDADPGWLSILKAHYGAIALGEYAAMSAEARMARFGRAPGMRNMATFGMLDENRHGQLQLYFPHDYCPKDRQFDWAHKA
YHTNQWGAIAARSTFDDLFMSRSAIEIAVMLTFAFETGFTNMQFLGLAADAAEAGDFTFASLISSIQTDESRHAQIGGPA
LQILIANGRKEQAQQLVDVAIARAWRLFSLLTGTSMDYATPLQHRKESFKEFMTEWIVGQFERTLIDLGLDLPWYWDQMI
NEFDYQHHAYQMGIWFWRPTVWWNPAAGMTPDCRDWLEEKYPGWNDTFGKAWDVIIDNLLAGRKELTVPETLPIVCNMSQ
LPICAVPGNGWNVRDYPLEYNGRTYHFNSEIDRWVFQQDPVRYRDHLTLVDRFLAGHIQPPDLGGALRYMNLAPGEIGDD
AHQYAWVEAYRRQREQNKAA

Specific function: Hydroxylates toluene to form p-cresol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR003430
- InterPro:   IPR012348
- InterPro:   IPR007029 [H]

Pfam domain/function: PF02332 Phenol_Hydrox; PF04945 YHS [H]

EC number: NA

Molecular weight: Translated: 57643; Mature: 57511

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLERAAWYDIARTTNWTPTYVAESDLFPEVMAGAQGVPMEIWETYDEPYKTSYPEYVR
CCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCHHEE
IQREKDAGAYSVKAALERSRMFEDADPGWLSILKAHYGAIALGEYAAMSAEARMARFGRA
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCC
PGMRNMATFGMLDENRHGQLQLYFPHDYCPKDRQFDWAHKAYHTNQWGAIAARSTFDDLF
CCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEHHHHHHHHH
MSRSAIEIAVMLTFAFETGFTNMQFLGLAADAAEAGDFTFASLISSIQTDESRHAQIGGP
HCCHHHEEEEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
ALQILIANGRKEQAQQLVDVAIARAWRLFSLLTGTSMDYATPLQHRKESFKEFMTEWIVG
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFERTLIDLGLDLPWYWDQMINEFDYQHHAYQMGIWFWRPTVWWNPAAGMTPDCRDWLEE
HHHHHHHHHCCCCCHHHHHHHHHHCHHHHHEEEEEEEECCCEEECCCCCCCCHHHHHHHH
KYPGWNDTFGKAWDVIIDNLLAGRKELTVPETLPIVCNMSQLPICAVPGNGWNVRDYPLE
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCEE
YNGRTYHFNSEIDRWVFQQDPVRYRDHLTLVDRFLAGHIQPPDLGGALRYMNLAPGEIGD
ECCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
DAHQYAWVEAYRRQREQNKAA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALLERAAWYDIARTTNWTPTYVAESDLFPEVMAGAQGVPMEIWETYDEPYKTSYPEYVR
CHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCHHEE
IQREKDAGAYSVKAALERSRMFEDADPGWLSILKAHYGAIALGEYAAMSAEARMARFGRA
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCC
PGMRNMATFGMLDENRHGQLQLYFPHDYCPKDRQFDWAHKAYHTNQWGAIAARSTFDDLF
CCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEHHHHHHHHH
MSRSAIEIAVMLTFAFETGFTNMQFLGLAADAAEAGDFTFASLISSIQTDESRHAQIGGP
HCCHHHEEEEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
ALQILIANGRKEQAQQLVDVAIARAWRLFSLLTGTSMDYATPLQHRKESFKEFMTEWIVG
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFERTLIDLGLDLPWYWDQMINEFDYQHHAYQMGIWFWRPTVWWNPAAGMTPDCRDWLEE
HHHHHHHHHCCCCCHHHHHHHHHHCHHHHHEEEEEEEECCCEEECCCCCCCCHHHHHHHH
KYPGWNDTFGKAWDVIIDNLLAGRKELTVPETLPIVCNMSQLPICAVPGNGWNVRDYPLE
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCEE
YNGRTYHFNSEIDRWVFQQDPVRYRDHLTLVDRFLAGHIQPPDLGGALRYMNLAPGEIGD
ECCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
DAHQYAWVEAYRRQREQNKAA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885512 [H]