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The map label for this gene is dmlR [H]

Identifier: 73538858

GI number: 73538858

Start: 1767174

End: 1768133

Strand: Direct

Name: dmlR [H]

Synonym: Reut_B5033

Alternate gene names: 73538858

Gene position: 1767174-1768133 (Clockwise)

Preceding gene: 73538856

Following gene: 73538860

Centisome position: 64.82

GC content: 67.4

Gene sequence:

>960_bases
ATGGAAACTCAGAATGCGCTGTCAGCGAACAATGACCCGTTCCTGCGGGACTTGCGGCTGTTCTGCACCGTAGCGCAGCG
TTCGAGCTTCATTGCCGCCGCGCATGAGATGGGCATCTCGCCCGCGCATGTGAGCAAGCGCATCGCCATGCTGGAGGCGA
TGCTCGGCACCCGGCTCTTTCATCGCACGACGCGACGCGTCAGCGTGACGGCGGAGGGCGAGAACGCGTTTGCGTGGGCG
CGGAAGATCCTGGAGGACGTGGATGGCATGCTCGAAGCCGTCGGCGGCGCCGGCAGGGCCGACAGCGAGCCGCGCGGGCT
GCTGCGCATTGCCGCAAGCTTCCGGCTCGGACGGCGTTTCGTGGCGCCGGTGCTGGCGCTGCTGCGGCAACAGCATCCGG
GGCTGGAGGTATGGCTGGAGCTGCTGGACCGGCGCGTGGACCTGATCGGCGAGAACTTCGATCTCGATATCCGCATCGGC
GAAGTTGAGGAGCCGCACCTGATTGCGAGCCACATCGCCAGCGGCAGCCGCGTGCTGTGTGCGGCGCCGGCCTACCTGGC
ACGGCGCGGCACGCCGCGCCATCTGGCCGAGCTTGCGCAGCACGATTGCCTGCTCCACCGCGAGCGCGACCAGGCCTTTG
GCGTGTGGCGGCTGCAAGGCCCGGCCGGCATGGAGATGGTCAAGGTGACCGGCCCGATGGCGTCCAACCACAGCGACGTC
ACGCGCCAGTGGGCCCATGAAGGCTACGGCATCATCATGGCGTCGGTCTGGGATGTGGCCGACAGCATCCGCGAAGGCGA
ACTCATGCAGGTGCTGCCGGCCTGGCAGCAGCCGGCCGATGTCTGGGCCGTGACCAGCGCGCGCGCATCTTCATCGGCGC
GCATGCGCGTGTGCATCGAATTTCTCAAGGACCGCCTGTCGCAGGGGCCGTTCGCGCTGGCGAGTTCATTGCACAAGTAA

Upstream 100 bases:

>100_bases
TACCGTCTTCATGTCGTCTCCTGAAATGAACGCGGCCGCGCCTCTGGCGCGGCCTTCGTCGATAGCCTATCGTTAAGCCT
GCGAATAACAATCCGGCAAT

Downstream 100 bases:

>100_bases
GCCACTGCGCTTAGCACGGCAGGCGCATGGGCGCTACAGCGTACGCATGATCGACAGCGCGGTGTCCTGCAGCACCTGCA
GCACGCGCGCGCATGCCGCT

Product: regulatory protein LysR

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
METQNALSANNDPFLRDLRLFCTVAQRSSFIAAAHEMGISPAHVSKRIAMLEAMLGTRLFHRTTRRVSVTAEGENAFAWA
RKILEDVDGMLEAVGGAGRADSEPRGLLRIAASFRLGRRFVAPVLALLRQQHPGLEVWLELLDRRVDLIGENFDLDIRIG
EVEEPHLIASHIASGSRVLCAAPAYLARRGTPRHLAELAQHDCLLHRERDQAFGVWRLQGPAGMEMVKVTGPMASNHSDV
TRQWAHEGYGIIMASVWDVADSIREGELMQVLPAWQQPADVWAVTSARASSSARMRVCIEFLKDRLSQGPFALASSLHK

Sequences:

>Translated_319_residues
METQNALSANNDPFLRDLRLFCTVAQRSSFIAAAHEMGISPAHVSKRIAMLEAMLGTRLFHRTTRRVSVTAEGENAFAWA
RKILEDVDGMLEAVGGAGRADSEPRGLLRIAASFRLGRRFVAPVLALLRQQHPGLEVWLELLDRRVDLIGENFDLDIRIG
EVEEPHLIASHIASGSRVLCAAPAYLARRGTPRHLAELAQHDCLLHRERDQAFGVWRLQGPAGMEMVKVTGPMASNHSDV
TRQWAHEGYGIIMASVWDVADSIREGELMQVLPAWQQPADVWAVTSARASSSARMRVCIEFLKDRLSQGPFALASSLHK
>Mature_319_residues
METQNALSANNDPFLRDLRLFCTVAQRSSFIAAAHEMGISPAHVSKRIAMLEAMLGTRLFHRTTRRVSVTAEGENAFAWA
RKILEDVDGMLEAVGGAGRADSEPRGLLRIAASFRLGRRFVAPVLALLRQQHPGLEVWLELLDRRVDLIGENFDLDIRIG
EVEEPHLIASHIASGSRVLCAAPAYLARRGTPRHLAELAQHDCLLHRERDQAFGVWRLQGPAGMEMVKVTGPMASNHSDV
TRQWAHEGYGIIMASVWDVADSIREGELMQVLPAWQQPADVWAVTSARASSSARMRVCIEFLKDRLSQGPFALASSLHK

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=47.4916387959866, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=39.5904436860068, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=29.3918918918919, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=28.3276450511945, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=30.0341296928328, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.1786941580756, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787589, Length=261, Percent_Identity=26.0536398467433, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=26.5384615384615, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790011, Length=279, Percent_Identity=24.7311827956989, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35338; Mature: 35338

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METQNALSANNDPFLRDLRLFCTVAQRSSFIAAAHEMGISPAHVSKRIAMLEAMLGTRLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HRTTRRVSVTAEGENAFAWARKILEDVDGMLEAVGGAGRADSEPRGLLRIAASFRLGRRF
HHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VAPVLALLRQQHPGLEVWLELLDRRVDLIGENFDLDIRIGEVEEPHLIASHIASGSRVLC
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEE
AAPAYLARRGTPRHLAELAQHDCLLHRERDQAFGVWRLQGPAGMEMVKVTGPMASNHSDV
ECHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHH
TRQWAHEGYGIIMASVWDVADSIREGELMQVLPAWQQPADVWAVTSARASSSARMRVCIE
HHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEEHHHHCCCHHHHHHHHH
FLKDRLSQGPFALASSLHK
HHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
METQNALSANNDPFLRDLRLFCTVAQRSSFIAAAHEMGISPAHVSKRIAMLEAMLGTRLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
HRTTRRVSVTAEGENAFAWARKILEDVDGMLEAVGGAGRADSEPRGLLRIAASFRLGRRF
HHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
VAPVLALLRQQHPGLEVWLELLDRRVDLIGENFDLDIRIGEVEEPHLIASHIASGSRVLC
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEE
AAPAYLARRGTPRHLAELAQHDCLLHRERDQAFGVWRLQGPAGMEMVKVTGPMASNHSDV
ECHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHH
TRQWAHEGYGIIMASVWDVADSIREGELMQVLPAWQQPADVWAVTSARASSSARMRVCIE
HHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEEHHHHCCCHHHHHHHHH
FLKDRLSQGPFALASSLHK
HHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]