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The map label for this gene is narI [H]

Identifier: 73538830

GI number: 73538830

Start: 1739691

End: 1740374

Strand: Direct

Name: narI [H]

Synonym: Reut_B5005

Alternate gene names: 73538830

Gene position: 1739691-1740374 (Clockwise)

Preceding gene: 73538829

Following gene: 73538831

Centisome position: 63.81

GC content: 62.87

Gene sequence:

>684_bases
ATGAACCTGATCAACCAATTCCTCTTCGGCATCTACCCGTACATCGCGCTCGCCATCTTCCTGTTCGGCAGCCTCGCGCG
CTTCGAGCGCGAGCAGTACACCTGGAAGACTGACAGCTCGCAGGTGCTGTATCGCGGCAACCTGCGCACGGGCAACATCC
TGTTCCACGTCGGCATCATCGGGCTGTTTTTCGGCCATCTGGCCGGTCTGCTGACGCCCGTGGCAGTGTGGGATGCGCTT
GGTGTTTCGCACAGCCTCAAGCAGGCTGTGGCGATGACCGCCGGCGGCGTGATGGGCACCATGTGCCTGGCCGGCCTGCT
GATCCTGCTGCATCGCCGCCTGACCAACGCGCGCGTATCGGCAGTGACCCGCACGGGCGACAAGGTATTGCTGTTGTGGC
TGCTGGTGACGCTGCTGCTAGGGCTGTCGACCATCTTCGAATCGGCCAGCCATATGGACGGCCACATGATGGTGCAGCTG
ATGACTTGGGCCCAGCACATCGTCACGCTGCGCGGCGATGCCGCAAGCTTCGTCGCCGAAGCCCCGCTCCTGTTCAAGGT
GCACCTGTTCATGGGCATGTCGCTGTTCGTGATCTTCCCGTTCACGCGCCTCGTCCATGTCTGGAGCGGTTTCGCTTCCG
TGGGCTATCTCGGCCGCGCGTGGCAACTCGTGCGCAGCCGTTAA

Upstream 100 bases:

>100_bases
CCCGCCACTGGCGCGCAGGCCGTGAAGTTCTATCCGCGTGGCACAACAGCGCAGCGCGCGCCGGTGTCCCCCGTCTGCTA
AGCATGCCAAGGTTACGACC

Downstream 100 bases:

>100_bases
GCGCATCCATCAGAACCGGAGAACACCATGCCAGTCACCGTCAATGGCGTAGAACTGACCGATGACGATATCGAACGCGA
GATGGACCAACACCGCGATG

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNLINQFLFGIYPYIALAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGIIGLFFGHLAGLLTPVAVWDAL
GVSHSLKQAVAMTAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHIVTLRGDAASFVAEAPLLFKVHLFMGMSLFVIFPFTRLVHVWSGFASVGYLGRAWQLVRSR

Sequences:

>Translated_227_residues
MNLINQFLFGIYPYIALAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGIIGLFFGHLAGLLTPVAVWDAL
GVSHSLKQAVAMTAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHIVTLRGDAASFVAEAPLLFKVHLFMGMSLFVIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
>Mature_227_residues
MNLINQFLFGIYPYIALAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGIIGLFFGHLAGLLTPVAVWDAL
GVSHSLKQAVAMTAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHIVTLRGDAASFVAEAPLLFKVHLFMGMSLFVIFPFTRLVHVWSGFASVGYLGRAWQLVRSR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=46.6960352422907, Blast_Score=200, Evalue=6e-53,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=45.8149779735683, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25228; Mature: 25228

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLINQFLFGIYPYIALAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLAGLLTPVAVWDALGVSHSLKQAVAMTAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHIVTLRGDAASFVAE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
APLLFKVHLFMGMSLFVIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLINQFLFGIYPYIALAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLAGLLTPVAVWDALGVSHSLKQAVAMTAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHIVTLRGDAASFVAE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
APLLFKVHLFMGMSLFVIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]