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The map label for this gene is mdeA [H]

Identifier: 73538793

GI number: 73538793

Start: 1695417

End: 1696829

Strand: Direct

Name: mdeA [H]

Synonym: Reut_B4968

Alternate gene names: 73538793

Gene position: 1695417-1696829 (Clockwise)

Preceding gene: 73538789

Following gene: 73538794

Centisome position: 62.19

GC content: 65.32

Gene sequence:

>1413_bases
ATGGTTCTTTCCCCCACATGGCCTGAGCCACCCCGCGCTGCTACCATCCCCCAAGCCCGACGCATCCGTTCGCCGCGGCT
CCATCCGGCATCTTCCGCACGCGTGCCGGTCCAGCACCTCTCCAAGAACAACCCGAACATGAGACTTGAAACCCTTGCCG
TCCACGGCGGCTACTCGCCCGATCCGACCACGCGTGCCGTAGCGGTACCGATCTATCAAACCGTCGCCTACTCGTTCGAC
AGCGCCCAGCATGGCGCCGACCTGTTTGACCTGAAGGTGCCAGGCAATATCTACAGCCGCATCATGAACCCGACCAACGA
CGTGCTGGAGCAGCGCCTGGCCGCGCTCGAAGGCGGCGTCGGCGCACTTGCGCTGGCTTCGGGCATGGCGGCCATCACCT
ACGCGATCCAGACCATCGCGGAGGCCGGCGACAACATCGTCTCATCGAGCCAGCTTTACGGCGGCACCTATAACCTGCTC
GCGCATACGCTGCCACAGTCGGGCATCGAAACGCGCTTTGCCGATGGCCGCAATCCGGCGAGCTTTGGCGACCTGATCGA
CGAACGTACCAAGGCCGTTTTCGTGGAATCGGTCGGCAATCCGCTCGGCAACGTGGTCGATATCGAAGCGATTGCGCGCG
TGGCGCACAACCATGGCGTGCCCCTGATCGTCGACAACACCGTGCCCTCTCCGTATCTGTGCCGGCCGTTCGAGCATGGC
GCGGATATCGTCGTGCATTCGCTGACCAAGTACCTGGGCGGCCACGGCAACAGCGTGGGCGGCGCGATCGTGGACAGCGG
GAAGTTCCCGTGGGCCAAGCACAAGGACCGCTTCAAGCGCCTGAACGAGCCCGACGTGTCATACCACGGCGTGGTCTATA
CCGAGGCACTGGGCCCAGCGGCCTACATCGGCCGCGCGCGCGTGGTGCCCCTGCGCAATACCGGCGCGGCGCTGTCGCCG
TTCAACGCGTTCCTCATCCTGCAAGGCATCGAGACGCTGGCGCTGCGCCTGGACCGCATCACCGAGAACGCGCTGGCTAT
CGCTCACCACCTGCAGAAGCATCCGAAGGTGGCATGGGTCAACTTCGCAGGCCTGCCGGAGCACGCTGATCACGCGCTGG
TGCAGAAGTACCTCGGCGGCCGCGGCCCCGGCATCCTGTCGTTCGGCCTGAAGGCGGGCGGACGTGAAGGCGGCGCGCGT
TTCCTCGACGCGCTGCAGCTGTTCACGCGCCTGGTCAACATTGGCGATGCCAAGTCGCTGGCTACGCACCCGGCCTCGAC
CACGCACCGCCAGCTCAATGCCGACGAGCTCAAGGCCGCCGGCGTGAGCGAAGACATGGTGCGCCTGTCGATCGGCATCG
AGCATATCGATGACCTGATCGAAGACCTCGACCGCGCCCTCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CTCGGCGGGTGATCGCTGTTTTTGCCGGGGTCTTCGGCGGGGGCTTGCGGTTCGGGCCCACAACCTCAAAAAGTCCCAAA
CGCGCATAAGCAAATGCAGT

Downstream 100 bases:

>100_bases
GCGTCGTGCCGCTTGCCGCGCTCGCTCGATGATGTGGACTATGCGTTCGTCAACGGCAACTTCGCGCTGGCTTCCGGTTT
GAAACTGACCTCGGCGCTGG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVLSPTWPEPPRAATIPQARRIRSPRLHPASSARVPVQHLSKNNPNMRLETLAVHGGYSPDPTTRAVAVPIYQTVAYSFD
SAQHGADLFDLKVPGNIYSRIMNPTNDVLEQRLAALEGGVGALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLL
AHTLPQSGIETRFADGRNPASFGDLIDERTKAVFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHG
ADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAKHKDRFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNTGAALSP
FNAFLILQGIETLALRLDRITENALAIAHHLQKHPKVAWVNFAGLPEHADHALVQKYLGGRGPGILSFGLKAGGREGGAR
FLDALQLFTRLVNIGDAKSLATHPASTTHRQLNADELKAAGVSEDMVRLSIGIEHIDDLIEDLDRALEAA

Sequences:

>Translated_470_residues
MVLSPTWPEPPRAATIPQARRIRSPRLHPASSARVPVQHLSKNNPNMRLETLAVHGGYSPDPTTRAVAVPIYQTVAYSFD
SAQHGADLFDLKVPGNIYSRIMNPTNDVLEQRLAALEGGVGALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLL
AHTLPQSGIETRFADGRNPASFGDLIDERTKAVFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHG
ADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAKHKDRFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNTGAALSP
FNAFLILQGIETLALRLDRITENALAIAHHLQKHPKVAWVNFAGLPEHADHALVQKYLGGRGPGILSFGLKAGGREGGAR
FLDALQLFTRLVNIGDAKSLATHPASTTHRQLNADELKAAGVSEDMVRLSIGIEHIDDLIEDLDRALEAA
>Mature_470_residues
MVLSPTWPEPPRAATIPQARRIRSPRLHPASSARVPVQHLSKNNPNMRLETLAVHGGYSPDPTTRAVAVPIYQTVAYSFD
SAQHGADLFDLKVPGNIYSRIMNPTNDVLEQRLAALEGGVGALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLL
AHTLPQSGIETRFADGRNPASFGDLIDERTKAVFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHG
ADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAKHKDRFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNTGAALSP
FNAFLILQGIETLALRLDRITENALAIAHHLQKHPKVAWVNFAGLPEHADHALVQKYLGGRGPGILSFGLKAGGREGGAR
FLDALQLFTRLVNIGDAKSLATHPASTTHRQLNADELKAAGVSEDMVRLSIGIEHIDDLIEDLDRALEAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.9881796690307, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=31.6784869976359, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.3317535545024, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=34.7619047619048, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=28.169014084507, Blast_Score=129, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=31.0679611650485, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=35.377358490566, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=429, Percent_Identity=34.7319347319347, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=49.0740740740741, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319307, Length=379, Percent_Identity=34.5646437994723, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=451, Percent_Identity=29.490022172949, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=28.3707865168539, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=436, Percent_Identity=26.1467889908257, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=35.0588235294118, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 50414; Mature: 50414

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSPTWPEPPRAATIPQARRIRSPRLHPASSARVPVQHLSKNNPNMRLETLAVHGGYSP
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECCCCC
DPTTRAVAVPIYQTVAYSFDSAQHGADLFDLKVPGNIYSRIMNPTNDVLEQRLAALEGGV
CCCCCEEEEHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCC
GALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQSGIETRFADGRNPA
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SFGDLIDERTKAVFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHG
HHHHHHHHHHHEEHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
ADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAKHKDRFKRLNEPDVSYHGVVYTEALGPA
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCH
AYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALAIAHHLQKHPKVAWV
HHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NFAGLPEHADHALVQKYLGGRGPGILSFGLKAGGREGGARFLDALQLFTRLVNIGDAKSL
EECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
ATHPASTTHRQLNADELKAAGVSEDMVRLSIGIEHIDDLIEDLDRALEAA
CCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVLSPTWPEPPRAATIPQARRIRSPRLHPASSARVPVQHLSKNNPNMRLETLAVHGGYSP
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEEEECCCCC
DPTTRAVAVPIYQTVAYSFDSAQHGADLFDLKVPGNIYSRIMNPTNDVLEQRLAALEGGV
CCCCCEEEEHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCC
GALALASGMAAITYAIQTIAEAGDNIVSSSQLYGGTYNLLAHTLPQSGIETRFADGRNPA
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SFGDLIDERTKAVFVESVGNPLGNVVDIEAIARVAHNHGVPLIVDNTVPSPYLCRPFEHG
HHHHHHHHHHHEEHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
ADIVVHSLTKYLGGHGNSVGGAIVDSGKFPWAKHKDRFKRLNEPDVSYHGVVYTEALGPA
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCH
AYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRLDRITENALAIAHHLQKHPKVAWV
HHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NFAGLPEHADHALVQKYLGGRGPGILSFGLKAGGREGGARFLDALQLFTRLVNIGDAKSL
EECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
ATHPASTTHRQLNADELKAAGVSEDMVRLSIGIEHIDDLIEDLDRALEAA
CCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]