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The map label for this gene is nasD [H]

Identifier: 73538687

GI number: 73538687

Start: 1571871

End: 1573112

Strand: Direct

Name: nasD [H]

Synonym: Reut_B4862

Alternate gene names: 73538687

Gene position: 1571871-1573112 (Clockwise)

Preceding gene: 73538686

Following gene: 73538688

Centisome position: 57.66

GC content: 68.68

Gene sequence:

>1242_bases
ATGACGCCATCCGCCCCCTCGCAGTCCCGCCCCCGGCTGATCGTCGTCGGCAACGGCATGGCCGGCATGCGCACGGTCGA
GGAACTGCTCAAGCTGGCCCCGGACCTGTACGACATCACCGTGTTCGGCGCGGAGCCGCACGGCAACTACAACCGCATCC
TGCTGTCGCCGGTGCTGGCCGGCGAGAAGACCGTCGACGACATCATGCTCAATACGCGAGAGTGGTATGCGCAGAACGGC
ATCGAACTGCTGGCGGGCGACCCCGTGGTGGCGATCGATCGCCCGCGCCGCACGGTGCGTGCGCAGTCCGGGCGCGAGGT
GCGCTATGACCGCCTGCTGCTCGCCACCGGCTCGAAGCCTTTCATCCTGCCGGTACCCGGCCACCAGCTCGACGGCGTGA
TCGCGTTTCGCGATATCCAGGACGTGGAAATCATGCTCGACGCCGCGCGTAACCACCGCCACGCGGTGGTGATCGGCGGC
GGCCTGCTCGGGCTGGAGGCCGCCAACGGCCTGCTGCGCCAGGGCATGGATGTCACCGTCGTGCATCTGTGCGACAGCCT
GATGGAACGCCAGCTCGACAAGCCCGCCGCCACGCTGCTCAAGGGCGCGCTCGAACGCAAGGGCCTGCGCTTCATGCTGG
GCGCGCAGACCGCGGAGATTCTAGGCGATACGCGCGTGACCGGCGTGCGTTTCAAGGACGGCAACGAGATCCCGGCCGAC
CTGGTGGTGATGACCGCCGGCGTGCGCCCGAACATCGAGCTGGCGGCCGGTGCAGGCCTGCGCTGCGAACGCGCCATCGT
GGTCGACGACACGCTGCAGACCTACGATCCGCGCATCTATGCCGTCGGTGAATGCGTTCAGCACCGCCAGGCCACGTTCG
GACTGGTGGCGCCGATCTGGGACCAGGCGCGCGTGTGCGCCGCGCACCTGGCCGGTGCGGGCCATCGCCGCTATATCCAG
CAGGCCACCGCCACGAAGCTCAAGGTCACCGGCGTGGATCTCTATTCCGCCGGCGATTTCATCGGCGGCGAAGGCAGCGA
GGACCTCGTGCTGCGCGACGCACGGCGCGGCGTCTACAAGCGGCTGGTACTGCAGGACGGGCGCCTGATCGGTGCCGTGC
TCTATGGCGACGTGCAGGACGGGCCGTGGTACTTCGACCTGATCCAGCAATGCGCACCGGTCGCGGCGCTGCGCCAGCGC
CTGCTGTTCGGCAAGGCCCAATGCGAGGCGCTCGCTGCCTGA

Upstream 100 bases:

>100_bases
GACGGCAAGGTATGGATCGCCGCCAATGTAACGCTGCGCGAAGCCGAAGAAGAACTCGCCGCCTGACACTGCAACCATGT
TCCCGATGGAGACCGCGCGC

Downstream 100 bases:

>100_bases
ACGCCATCATCCGACGACAACGACATCGCCCCACGGAGAACGCGTGAATCTGTCCGACATCCCGGTGGTATCCGCCACCC
CGACCACCACCACCGCCACC

Product: assimilatory nitrate reductase (NADH) subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTPSAPSQSRPRLIVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTREWYAQNG
IELLAGDPVVAIDRPRRTVRAQSGREVRYDRLLLATGSKPFILPVPGHQLDGVIAFRDIQDVEIMLDAARNHRHAVVIGG
GLLGLEAANGLLRQGMDVTVVHLCDSLMERQLDKPAATLLKGALERKGLRFMLGAQTAEILGDTRVTGVRFKDGNEIPAD
LVVMTAGVRPNIELAAGAGLRCERAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYIQ
QATATKLKVTGVDLYSAGDFIGGEGSEDLVLRDARRGVYKRLVLQDGRLIGAVLYGDVQDGPWYFDLIQQCAPVAALRQR
LLFGKAQCEALAA

Sequences:

>Translated_413_residues
MTPSAPSQSRPRLIVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTREWYAQNG
IELLAGDPVVAIDRPRRTVRAQSGREVRYDRLLLATGSKPFILPVPGHQLDGVIAFRDIQDVEIMLDAARNHRHAVVIGG
GLLGLEAANGLLRQGMDVTVVHLCDSLMERQLDKPAATLLKGALERKGLRFMLGAQTAEILGDTRVTGVRFKDGNEIPAD
LVVMTAGVRPNIELAAGAGLRCERAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYIQ
QATATKLKVTGVDLYSAGDFIGGEGSEDLVLRDARRGVYKRLVLQDGRLIGAVLYGDVQDGPWYFDLIQQCAPVAALRQR
LLFGKAQCEALAA
>Mature_412_residues
TPSAPSQSRPRLIVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTREWYAQNGI
ELLAGDPVVAIDRPRRTVRAQSGREVRYDRLLLATGSKPFILPVPGHQLDGVIAFRDIQDVEIMLDAARNHRHAVVIGGG
LLGLEAANGLLRQGMDVTVVHLCDSLMERQLDKPAATLLKGALERKGLRFMLGAQTAEILGDTRVTGVRFKDGNEIPADL
VVMTAGVRPNIELAAGAGLRCERAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYIQQ
ATATKLKVTGVDLYSAGDFIGGEGSEDLVLRDARRGVYKRLVLQDGRLIGAVLYGDVQDGPWYFDLIQQCAPVAALRQRL
LFGKAQCEALAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=290, Percent_Identity=28.9655172413793, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI226437568, Length=290, Percent_Identity=28.9655172413793, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI65787454, Length=290, Percent_Identity=28.9655172413793, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4757732, Length=192, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI22202629, Length=192, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1789765, Length=390, Percent_Identity=36.9230769230769, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1789065, Length=328, Percent_Identity=28.0487804878049, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788892, Length=247, Percent_Identity=25.9109311740891, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=316, Percent_Identity=27.5316455696203, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI281359715, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281359713, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639250, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18543267, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639252, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639257, Length=311, Percent_Identity=25.7234726688103, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=23.8434163701068, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21358499, Length=208, Percent_Identity=29.8076923076923, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581020, Length=199, Percent_Identity=26.1306532663317, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28573993, Length=199, Percent_Identity=26.1306532663317, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 44898; Mature: 44767

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSAPSQSRPRLIVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLA
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHC
GEKTVDDIMLNTREWYAQNGIELLAGDPVVAIDRPRRTVRAQSGREVRYDRLLLATGSKP
CCCHHHHHHHCCHHHHHCCCCEEEECCCEEEECCCCHHHHCCCCCCEEEEEEEEEECCCC
FILPVPGHQLDGVIAFRDIQDVEIMLDAARNHRHAVVIGGGLLGLEAANGLLRQGMDVTV
EEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHCCCCCHH
VHLCDSLMERQLDKPAATLLKGALERKGLRFMLGAQTAEILGDTRVTGVRFKDGNEIPAD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHCCCEEEEEEECCCCCCCCE
LVVMTAGVRPNIELAAGAGLRCERAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIW
EEEEECCCCCCEEEECCCCCEECEEEEEECCHHHCCCEEEEHHHHHHHHHHHHEEECCHH
DQARVCAAHLAGAGHRRYIQQATATKLKVTGVDLYSAGDFIGGEGSEDLVLRDARRGVYK
HHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RLVLQDGRLIGAVLYGDVQDGPWYFDLIQQCAPVAALRQRLLFGKAQCEALAA
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TPSAPSQSRPRLIVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEEHHC
GEKTVDDIMLNTREWYAQNGIELLAGDPVVAIDRPRRTVRAQSGREVRYDRLLLATGSKP
CCCHHHHHHHCCHHHHHCCCCEEEECCCEEEECCCCHHHHCCCCCCEEEEEEEEEECCCC
FILPVPGHQLDGVIAFRDIQDVEIMLDAARNHRHAVVIGGGLLGLEAANGLLRQGMDVTV
EEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHCCCCCHH
VHLCDSLMERQLDKPAATLLKGALERKGLRFMLGAQTAEILGDTRVTGVRFKDGNEIPAD
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHCCCEEEEEEECCCCCCCCE
LVVMTAGVRPNIELAAGAGLRCERAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIW
EEEEECCCCCCEEEECCCCCEECEEEEEECCHHHCCCEEEEHHHHHHHHHHHHEEECCHH
DQARVCAAHLAGAGHRRYIQQATATKLKVTGVDLYSAGDFIGGEGSEDLVLRDARRGVYK
HHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
RLVLQDGRLIGAVLYGDVQDGPWYFDLIQQCAPVAALRQRLLFGKAQCEALAA
HHHHCCCCEEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]