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The map label for this gene is purU [H]

Identifier: 73538637

GI number: 73538637

Start: 1512539

End: 1513411

Strand: Direct

Name: purU [H]

Synonym: Reut_B4812

Alternate gene names: 73538637

Gene position: 1512539-1513411 (Clockwise)

Preceding gene: 73538630

Following gene: 73538648

Centisome position: 55.48

GC content: 62.54

Gene sequence:

>873_bases
ATGGACGCCCTGCCCCGCCCACAAACCTTCGTTCTCACGCTGTCGTGCCCGAGCGCCCCCGGCCAGGTGGCTGCGGTCGT
CGGCCTGCTGGAGCGCCACCGCTGCTACATCGACGAACTGACCGTCTTCGATGACGACCTGAGCAACCGCTTCTTCTTGC
GCTGTGTATTCCATCCAACCCACGCCCAGGCGCCAGGCATTGACGTGTTGCGGCAGGAGTTCACGCCGATAGCGGAGGGC
TTCGGCATGCAATGGGCGATCCACGACGCACTCGCGCGCCCGAAGGTACTGATCATGGTGTCCAGGCTCGAGCATTGCCT
CGCGGATCTGTTGTTCCGCTGGCGCATGGGCGAGTTGAAGATGGACATCGTGGGGATTGCTTCGAACCATCCCGACCTGG
AACCACTCGCCCGGCAGCATGATCTCCCGTTCCGGCATTTCCCGATCACGCCAGAGACGAAGGCAAAACAGGAAGCGCAG
TGGCTGGACCTGTTCGAGTCGAGCGGCGCCGAGCTTGTGATCCTTGCACGCTATATGCAGGTGCTGTCCCCCGAAACCAG
CGGAAAGCTCGCCAATCGCGCGATCAACATCCACCATTCTTTCCTGCCTGGCTTCAAGGGCGCGAAGCCGTATCACCAGG
CGCATGCGCGCGGCGTCAAGCTGATCGGCGCGACGGCGCATTTCGTCACCGACGATCTCGATGAAGGTCCCATCATCGAG
CAGGCGGTCGAGCGCGTCGATCATTCGTACCGGCCCGAGCAATTGCTCGCGGTTGGACGCGACGTCGAATGCATCACGCT
CGCACGCGCGGTCAAGGCCTTCATCGATCGACGGGTGTTCCTGAACGGCGACCGCACGGTCGTGTTCCAGTAA

Upstream 100 bases:

>100_bases
GGTTCTATCCAGCCTGGCATCCGGACTGCTCTACTTCGCGCATGGCACGCGCGAGCGCACGACGCGCCCGGCGGCCACAC
CTCTCCACCAAAGGAATTCC

Downstream 100 bases:

>100_bases
GCCCCAAAACACATCGGCCACGATCGCAATGCGACCGTGGCCGGAACCCTCCCTGACTTTCTTGAGGCGTTACGAACTAG
CGGTCGCCTTCGAGCCACGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDALPRPQTFVLTLSCPSAPGQVAAVVGLLERHRCYIDELTVFDDDLSNRFFLRCVFHPTHAQAPGIDVLRQEFTPIAEG
FGMQWAIHDALARPKVLIMVSRLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPITPETKAKQEAQ
WLDLFESSGAELVILARYMQVLSPETSGKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QAVERVDHSYRPEQLLAVGRDVECITLARAVKAFIDRRVFLNGDRTVVFQ

Sequences:

>Translated_290_residues
MDALPRPQTFVLTLSCPSAPGQVAAVVGLLERHRCYIDELTVFDDDLSNRFFLRCVFHPTHAQAPGIDVLRQEFTPIAEG
FGMQWAIHDALARPKVLIMVSRLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPITPETKAKQEAQ
WLDLFESSGAELVILARYMQVLSPETSGKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QAVERVDHSYRPEQLLAVGRDVECITLARAVKAFIDRRVFLNGDRTVVFQ
>Mature_290_residues
MDALPRPQTFVLTLSCPSAPGQVAAVVGLLERHRCYIDELTVFDDDLSNRFFLRCVFHPTHAQAPGIDVLRQEFTPIAEG
FGMQWAIHDALARPKVLIMVSRLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPITPETKAKQEAQ
WLDLFESSGAELVILARYMQVLSPETSGKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QAVERVDHSYRPEQLLAVGRDVECITLARAVKAFIDRRVFLNGDRTVVFQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=37.5886524822695, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788846, Length=151, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=151, Percent_Identity=33.7748344370861, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32668; Mature: 32668

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALPRPQTFVLTLSCPSAPGQVAAVVGLLERHRCYIDELTVFDDDLSNRFFLRCVFHPT
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCC
HAQAPGIDVLRQEFTPIAEGFGMQWAIHDALARPKVLIMVSRLEHCLADLLFRWRMGELK
CCCCCCHHHHHHHCCHHHHCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE
MDIVGIASNHPDLEPLARQHDLPFRHFPITPETKAKQEAQWLDLFESSGAELVILARYMQ
EEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHH
VLSPETSGKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
HHCCCCCCHHHHHEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHHH
QAVERVDHSYRPEQLLAVGRDVECITLARAVKAFIDRRVFLNGDRTVVFQ
HHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCEEEEEC
>Mature Secondary Structure
MDALPRPQTFVLTLSCPSAPGQVAAVVGLLERHRCYIDELTVFDDDLSNRFFLRCVFHPT
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCC
HAQAPGIDVLRQEFTPIAEGFGMQWAIHDALARPKVLIMVSRLEHCLADLLFRWRMGELK
CCCCCCHHHHHHHCCHHHHCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEE
MDIVGIASNHPDLEPLARQHDLPFRHFPITPETKAKQEAQWLDLFESSGAELVILARYMQ
EEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHH
VLSPETSGKLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
HHCCCCCCHHHHHEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHHH
QAVERVDHSYRPEQLLAVGRDVECITLARAVKAFIDRRVFLNGDRTVVFQ
HHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]