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The map label for this gene is pleD [H]

Identifier: 73538536

GI number: 73538536

Start: 1408394

End: 1408879

Strand: Reverse

Name: pleD [H]

Synonym: Reut_B4711

Alternate gene names: 73538536

Gene position: 1408879-1408394 (Counterclockwise)

Preceding gene: 73538538

Following gene: 73538534

Centisome position: 51.68

GC content: 59.26

Gene sequence:

>486_bases
ATGCGGCGTCCCTACAAGCGTGAGGATATCGGTAAGAGCATTGCCGGTGCCCCGTCGTTCGAGCCGCTCATCCAGTCCGA
CAATGGTTCCTACATTGGCACCGCGACGCTAAAGGCGATTGCGCAATGCATTACCAAGAATATCCGTCGCCCGGGAGATT
TCGTTGGCCGTTATGGTGGCGAAGAGTTCTCTGTGTTGCTGCCAAGCACGGACATGAACGGGGCAATCAGGATTGCGGAG
AACATTCGCGCAGCAGTGATGGCAACGGAGTTGACAAGTATGGGCGCGCGGCCTGTTCCGCTGAGCGTGAGTATTGGGGT
GGCGACTTTTGACGGCAAGAATGCTCGCGCGGAATCTTCTGAACAGCTTCTGCGCCAGGCCGACCGCCGTCTCTACCAGG
CAAAGGCTGCTGGTCGTAACACGATCATGCCGCGCCAAGCGCATGGCCAGGCGGCGATGGTCGGGGATCCCCACCCGAGT
ACCTGA

Upstream 100 bases:

>100_bases
CGCCGTTGTTCATTGTGGGGGCGAGTGTTGCCGCCGTGACCATGGCATGCGTTGCCGCAGTCGCCCTTTATGAAATGCGT
CTGGCGACGGAACGGTACTG

Downstream 100 bases:

>100_bases
AAGCCGCGGCCAATCCAGTCGGAAGAAGCCGTCACACGCGGAGCACCGCCGCCCCGGCCAGACGGCCAGCGCGCAGGTCA
TCCAACGCCGCATTGGCACT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MRRPYKREDIGKSIAGAPSFEPLIQSDNGSYIGTATLKAIAQCITKNIRRPGDFVGRYGGEEFSVLLPSTDMNGAIRIAE
NIRAAVMATELTSMGARPVPLSVSIGVATFDGKNARAESSEQLLRQADRRLYQAKAAGRNTIMPRQAHGQAAMVGDPHPS
T

Sequences:

>Translated_161_residues
MRRPYKREDIGKSIAGAPSFEPLIQSDNGSYIGTATLKAIAQCITKNIRRPGDFVGRYGGEEFSVLLPSTDMNGAIRIAE
NIRAAVMATELTSMGARPVPLSVSIGVATFDGKNARAESSEQLLRQADRRLYQAKAAGRNTIMPRQAHGQAAMVGDPHPS
T
>Mature_161_residues
MRRPYKREDIGKSIAGAPSFEPLIQSDNGSYIGTATLKAIAQCITKNIRRPGDFVGRYGGEEFSVLLPSTDMNGAIRIAE
NIRAAVMATELTSMGARPVPLSVSIGVATFDGKNARAESSEQLLRQADRRLYQAKAAGRNTIMPRQAHGQAAMVGDPHPS
T

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=33.7748344370861, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87081881, Length=129, Percent_Identity=35.6589147286822, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082007, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 17301; Mature: 17301

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPYKREDIGKSIAGAPSFEPLIQSDNGSYIGTATLKAIAQCITKNIRRPGDFVGRYGG
CCCCCCHHHHCCHHCCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHCCCC
EEFSVLLPSTDMNGAIRIAENIRAAVMATELTSMGARPVPLSVSIGVATFDGKNARAESS
CEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHH
EQLLRQADRRLYQAKAAGRNTIMPRQAHGQAAMVGDPHPST
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MRRPYKREDIGKSIAGAPSFEPLIQSDNGSYIGTATLKAIAQCITKNIRRPGDFVGRYGG
CCCCCCHHHHCCHHCCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHCCCC
EEFSVLLPSTDMNGAIRIAENIRAAVMATELTSMGARPVPLSVSIGVATFDGKNARAESS
CEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHH
EQLLRQADRRLYQAKAAGRNTIMPRQAHGQAAMVGDPHPST
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA