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The map label for this gene is 73538528

Identifier: 73538528

GI number: 73538528

Start: 1399558

End: 1400856

Strand: Reverse

Name: 73538528

Synonym: Reut_B4702

Alternate gene names: NA

Gene position: 1400856-1399558 (Counterclockwise)

Preceding gene: 73538530

Following gene: 73538527

Centisome position: 51.39

GC content: 63.66

Gene sequence:

>1299_bases
ATGTCAGGCGCGACCACTTCAATGAGATGCCTGGAGACACCAAGGCACTTTCGCGTAATGGTCGACGGCATCCGACACAA
ACAGATTAGCGGCCGGATTCCCTCTGGCAGCCACAGCATGAATTCCTCAATGGGCAGTCTGGATAACTTTTCGGCGGCGG
CGGCGTGGGCGCCGATGGGGTCATTCTTGTCGGCCATGTGCGGCGCGCAGTTCGCCTACGCCTACGAACTGTTCCAGAGG
TCGATGCGCCAGTTCGGGCCGCCGTCATACCATCTGGACACTGTCTGCCAGGACGGCAAACACGTGCCGGTGACAATGAC
CGTCGTTGCCGAGACCCCGTTCTGCCGTTTGCTGCGCTTCTCGGTGGGCGACGGTCACGGCGTACGCCGGATGTTGTGCG
CACCGCTTGCCGGGCACCGGGCAGTCGTGGTTCGCGACGCCATCGAATCCATGCTGCTTGACGCGGACGTCTATGTCACC
GACTGGATTGATGCACGGGACGTACCGCGCGAAGCCGGCCACTTCGGCCTGGCAGACTGCGTATTGCTGCTCGAGCAGTT
CATGACGCTGCTCGATCCGGCGCGCCTTGATGTGGTGGCGATCTGCCAGGCGACCATTCCGTGCATTGCGGCCGTTTCTC
GCCTGGCATCCGGAGATGCGCCGGAAGTGAGGGCGCTGGTACTGATGGGCGGCCCGCTGGATGCAAGGCTTCACCCGACC
AGACTCGGCGACATCGCGGCCATGATGCCGCTGCCAGTGTTTCTCGCCCACTGCACAGGCGCCGTGCCGCACGAATATCG
CGGCGCGGGACGTATCGTGTATCCGGGCTTTCTGCAATTGCCCACGCTGGCATGTGGCCAGCCGGAGCGCCTTTCGGCGA
TGGTGTTCGCTGCGCTTCAGGGGCCATGGCCGGGGTCCCGGGCGCAGGCAGATCTGCGCCATGCAGCCGTAGCGGGGTAT
GTGGCGATGATGGACATGCCGGTTGAATTCGTGCTCGATACCATGCGCATCGTCTTCCGCGAGCACCTGTTGCCACGTGG
GTCGTGGTACGTCGGAGGAATGCCGGTGGATCCGGCAGCCATGCGGACAACACGGCTACTGACGGTTGAAGGGCAGCTCG
ATACGATCACCGGCGCCGGGCAGACCCACGCCGCGCAGACGCTGTGCCGTGGTCTGGAACGACGGTTGTGTACGCAAGTC
ACCGTTCCGGGTTGCGACCACTATGGGTTGTTTTCCGGCCCGGTGTGGCGAAGCCGCGTTTATCCCATGCTACGGAGCTG
GCTTAACGCGCCGGCGTGA

Upstream 100 bases:

>100_bases
GCCGTCGAGGCTGACTCGGCGAGGAGAAGTGGGGCGAAACGCAGTCGCTCGGAACGATATTGCGTTGCCGCACGCGGGGC
CGCATCTGTGGATTATCTTG

Downstream 100 bases:

>100_bases
CACCGGCGATCCTGACGTGAAGAGACGCTCTTGCGGAGAGGGTCGAGGCAGGCCCATTGGCACTGCCTTCCGAATCCTTC
AAGCTCGTCGTCTCGTGCTA

Product: polyhydroxyalkanoate depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly(3-Hydroxybutyrate) Depolymerase; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSGATTSMRCLETPRHFRVMVDGIRHKQISGRIPSGSHSMNSSMGSLDNFSAAAAWAPMGSFLSAMCGAQFAYAYELFQR
SMRQFGPPSYHLDTVCQDGKHVPVTMTVVAETPFCRLLRFSVGDGHGVRRMLCAPLAGHRAVVVRDAIESMLLDADVYVT
DWIDARDVPREAGHFGLADCVLLLEQFMTLLDPARLDVVAICQATIPCIAAVSRLASGDAPEVRALVLMGGPLDARLHPT
RLGDIAAMMPLPVFLAHCTGAVPHEYRGAGRIVYPGFLQLPTLACGQPERLSAMVFAALQGPWPGSRAQADLRHAAVAGY
VAMMDMPVEFVLDTMRIVFREHLLPRGSWYVGGMPVDPAAMRTTRLLTVEGQLDTITGAGQTHAAQTLCRGLERRLCTQV
TVPGCDHYGLFSGPVWRSRVYPMLRSWLNAPA

Sequences:

>Translated_432_residues
MSGATTSMRCLETPRHFRVMVDGIRHKQISGRIPSGSHSMNSSMGSLDNFSAAAAWAPMGSFLSAMCGAQFAYAYELFQR
SMRQFGPPSYHLDTVCQDGKHVPVTMTVVAETPFCRLLRFSVGDGHGVRRMLCAPLAGHRAVVVRDAIESMLLDADVYVT
DWIDARDVPREAGHFGLADCVLLLEQFMTLLDPARLDVVAICQATIPCIAAVSRLASGDAPEVRALVLMGGPLDARLHPT
RLGDIAAMMPLPVFLAHCTGAVPHEYRGAGRIVYPGFLQLPTLACGQPERLSAMVFAALQGPWPGSRAQADLRHAAVAGY
VAMMDMPVEFVLDTMRIVFREHLLPRGSWYVGGMPVDPAAMRTTRLLTVEGQLDTITGAGQTHAAQTLCRGLERRLCTQV
TVPGCDHYGLFSGPVWRSRVYPMLRSWLNAPA
>Mature_431_residues
SGATTSMRCLETPRHFRVMVDGIRHKQISGRIPSGSHSMNSSMGSLDNFSAAAAWAPMGSFLSAMCGAQFAYAYELFQRS
MRQFGPPSYHLDTVCQDGKHVPVTMTVVAETPFCRLLRFSVGDGHGVRRMLCAPLAGHRAVVVRDAIESMLLDADVYVTD
WIDARDVPREAGHFGLADCVLLLEQFMTLLDPARLDVVAICQATIPCIAAVSRLASGDAPEVRALVLMGGPLDARLHPTR
LGDIAAMMPLPVFLAHCTGAVPHEYRGAGRIVYPGFLQLPTLACGQPERLSAMVFAALQGPWPGSRAQADLRHAAVAGYV
AMMDMPVEFVLDTMRIVFREHLLPRGSWYVGGMPVDPAAMRTTRLLTVEGQLDTITGAGQTHAAQTLCRGLERRLCTQVT
VPGCDHYGLFSGPVWRSRVYPMLRSWLNAPA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47005; Mature: 46874

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGATTSMRCLETPRHFRVMVDGIRHKQISGRIPSGSHSMNSSMGSLDNFSAAAAWAPMG
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
SFLSAMCGAQFAYAYELFQRSMRQFGPPSYHLDTVCQDGKHVPVTMTVVAETPFCRLLRF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
SVGDGHGVRRMLCAPLAGHRAVVVRDAIESMLLDADVYVTDWIDARDVPREAGHFGLADC
HCCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCHHHH
VLLLEQFMTLLDPARLDVVAICQATIPCIAAVSRLASGDAPEVRALVLMGGPLDARLHPT
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
RLGDIAAMMPLPVFLAHCTGAVPHEYRGAGRIVYPGFLQLPTLACGQPERLSAMVFAALQ
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCHHHCCCCCCCCCHHHHHHHHHHHC
GPWPGSRAQADLRHAAVAGYVAMMDMPVEFVLDTMRIVFREHLLPRGSWYVGGMPVDPAA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHH
MRTTRLLTVEGQLDTITGAGQTHAAQTLCRGLERRLCTQVTVPGCDHYGLFSGPVWRSRV
HHEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YPMLRSWLNAPA
HHHHHHHHCCCC
>Mature Secondary Structure 
SGATTSMRCLETPRHFRVMVDGIRHKQISGRIPSGSHSMNSSMGSLDNFSAAAAWAPMG
CCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
SFLSAMCGAQFAYAYELFQRSMRQFGPPSYHLDTVCQDGKHVPVTMTVVAETPFCRLLRF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
SVGDGHGVRRMLCAPLAGHRAVVVRDAIESMLLDADVYVTDWIDARDVPREAGHFGLADC
HCCCCCHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCCHHHH
VLLLEQFMTLLDPARLDVVAICQATIPCIAAVSRLASGDAPEVRALVLMGGPLDARLHPT
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
RLGDIAAMMPLPVFLAHCTGAVPHEYRGAGRIVYPGFLQLPTLACGQPERLSAMVFAALQ
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCHHHCCCCCCCCCHHHHHHHHHHHC
GPWPGSRAQADLRHAAVAGYVAMMDMPVEFVLDTMRIVFREHLLPRGSWYVGGMPVDPAA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHH
MRTTRLLTVEGQLDTITGAGQTHAAQTLCRGLERRLCTQVTVPGCDHYGLFSGPVWRSRV
HHEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YPMLRSWLNAPA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA