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The map label for this gene is gcvA [H]

Identifier: 73538195

GI number: 73538195

Start: 1022751

End: 1023683

Strand: Direct

Name: gcvA [H]

Synonym: Reut_B4366

Alternate gene names: 73538195

Gene position: 1022751-1023683 (Clockwise)

Preceding gene: 73538193

Following gene: 73538197

Centisome position: 37.52

GC content: 66.67

Gene sequence:

>933_bases
ATGTCCGTACCGCTTGTCCGACTGTTCCCGCTCGACCTGCTCAAGGGCTTCGTCGCTGTCGGCCGGCGCATGAGCATCAC
GCAGGCGGCCGACGACCTTTGCCTCACGCAATCGGCGGTCAGCCGCCAGATCCATGCGCTGGAGGAGCAGTTGAAGGTGC
CCCTGTTCGTGCGCAAGCATCGCGGCGTGGCATTCACGGCCGAGGGAGAACGGCTGTTCCGCAGCGCCGACAGCGCGTTG
CAGCAACTGCAGGACGTCGTCGCCGAAGTGCGCCGCAACGAAGTCCCGCGGCCCGTCACGGTCACTGCGAGCATTGGCGT
CACGGGTTTATGGTTGCTGCCACGGCTCGGGAGCCTGCAACGGACGCATCCGCACCTCGATGTACGTCTGTCCGCGAGCA
ACCGCATCAGCGACCTGCGCGCCGAGGGCATCGACCTGGCCGTGCGTTATTGCCGCGACGCCGCGGCGCCGACCGATGCC
ATGCGCCTGTTCGGCGAGAACATCGCGCCGGTCGCGCATCCGTCGCTGCTGGCCGATGCCGGCCCGCATGACGATGTGCT
GCTGGAGTTGCCGCTGCTCGAGTTCGACGATCCGCGTCCGTGGCTGCAGTGGCGCGGCTGGCTGGAGCCACGCGGCTACC
GGCAGGCGAGCCAGCGCGGCATGCTTCGCTTCAACCAGTACGACCAGGTCATCCACGCCGCGCTGGCGGGGCAAGGCATT
GCGTTGGGCAGGCTTGAACTCATGCGGCCGCTGATTGAAGGCGGCCAGTTGGTGATCGTGCCGACCATTCGGTCGGCACC
GCCAAGCCCGAACGCTTACTGGCTGATCTTCGCCACGCCAGATCCGCGCAATGATGTCAGGCGCGTGGCCGAATGGATCT
GCGAACAGGCCGAGCAAACCGTCAGGATGTCCACGCATCCCGCAACCACCTGA

Upstream 100 bases:

>100_bases
AGCGATCTTTCGGTCGTTCTGCCATGCGTACGGCGCATGGCAAGGCAATCGCGGATCGTGCTGTAATGGAGCCATCGAAG
GTTTGTCAGGAGCGCCACGC

Downstream 100 bases:

>100_bases
GCCACGGCACGGCAACGCGGTGCCCCAGTGACGGCGCATCGATCTCGCACAATGCGGGGCCCGCTCCGTGCAGAGCGGCA
ACAAACACCTCGGCGTCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSVPLVRLFPLDLLKGFVAVGRRMSITQAADDLCLTQSAVSRQIHALEEQLKVPLFVRKHRGVAFTAEGERLFRSADSAL
QQLQDVVAEVRRNEVPRPVTVTASIGVTGLWLLPRLGSLQRTHPHLDVRLSASNRISDLRAEGIDLAVRYCRDAAAPTDA
MRLFGENIAPVAHPSLLADAGPHDDVLLELPLLEFDDPRPWLQWRGWLEPRGYRQASQRGMLRFNQYDQVIHAALAGQGI
ALGRLELMRPLIEGGQLVIVPTIRSAPPSPNAYWLIFATPDPRNDVRRVAEWICEQAEQTVRMSTHPATT

Sequences:

>Translated_310_residues
MSVPLVRLFPLDLLKGFVAVGRRMSITQAADDLCLTQSAVSRQIHALEEQLKVPLFVRKHRGVAFTAEGERLFRSADSAL
QQLQDVVAEVRRNEVPRPVTVTASIGVTGLWLLPRLGSLQRTHPHLDVRLSASNRISDLRAEGIDLAVRYCRDAAAPTDA
MRLFGENIAPVAHPSLLADAGPHDDVLLELPLLEFDDPRPWLQWRGWLEPRGYRQASQRGMLRFNQYDQVIHAALAGQGI
ALGRLELMRPLIEGGQLVIVPTIRSAPPSPNAYWLIFATPDPRNDVRRVAEWICEQAEQTVRMSTHPATT
>Mature_309_residues
SVPLVRLFPLDLLKGFVAVGRRMSITQAADDLCLTQSAVSRQIHALEEQLKVPLFVRKHRGVAFTAEGERLFRSADSALQ
QLQDVVAEVRRNEVPRPVTVTASIGVTGLWLLPRLGSLQRTHPHLDVRLSASNRISDLRAEGIDLAVRYCRDAAAPTDAM
RLFGENIAPVAHPSLLADAGPHDDVLLELPLLEFDDPRPWLQWRGWLEPRGYRQASQRGMLRFNQYDQVIHAALAGQGIA
LGRLELMRPLIEGGQLVIVPTIRSAPPSPNAYWLIFATPDPRNDVRRVAEWICEQAEQTVRMSTHPATT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=31.3131313131313, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786448, Length=268, Percent_Identity=32.4626865671642, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=28.0769230769231, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=32.4324324324324, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=27.4576271186441, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=251, Percent_Identity=27.8884462151394, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=256, Percent_Identity=25.390625, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=241, Percent_Identity=28.6307053941909, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786713, Length=295, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34580; Mature: 34449

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPLVRLFPLDLLKGFVAVGRRMSITQAADDLCLTQSAVSRQIHALEEQLKVPLFVRKH
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RGVAFTAEGERLFRSADSALQQLQDVVAEVRRNEVPRPVTVTASIGVTGLWLLPRLGSLQ
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCC
RTHPHLDVRLSASNRISDLRAEGIDLAVRYCRDAAAPTDAMRLFGENIAPVAHPSLLADA
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHCC
GPHDDVLLELPLLEFDDPRPWLQWRGWLEPRGYRQASQRGMLRFNQYDQVIHAALAGQGI
CCCCCEEEEECCEECCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC
ALGRLELMRPLIEGGQLVIVPTIRSAPPSPNAYWLIFATPDPRNDVRRVAEWICEQAEQT
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
VRMSTHPATT
HCCCCCCCCC
>Mature Secondary Structure 
SVPLVRLFPLDLLKGFVAVGRRMSITQAADDLCLTQSAVSRQIHALEEQLKVPLFVRKH
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RGVAFTAEGERLFRSADSALQQLQDVVAEVRRNEVPRPVTVTASIGVTGLWLLPRLGSLQ
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCC
RTHPHLDVRLSASNRISDLRAEGIDLAVRYCRDAAAPTDAMRLFGENIAPVAHPSLLADA
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHCC
GPHDDVLLELPLLEFDDPRPWLQWRGWLEPRGYRQASQRGMLRFNQYDQVIHAALAGQGI
CCCCCEEEEECCEECCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC
ALGRLELMRPLIEGGQLVIVPTIRSAPPSPNAYWLIFATPDPRNDVRRVAEWICEQAEQT
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
VRMSTHPATT
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]