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The map label for this gene is hipO [H]

Identifier: 73538130

GI number: 73538130

Start: 958967

End: 960205

Strand: Direct

Name: hipO [H]

Synonym: Reut_B4300

Alternate gene names: 73538130

Gene position: 958967-960205 (Clockwise)

Preceding gene: 73538128

Following gene: 73538131

Centisome position: 35.18

GC content: 65.38

Gene sequence:

>1239_bases
ATGGATGCCGGACGCGTGCGCGTGGAGAATAGCTCCACGATCCAGCCCCGCCACGCCATGAACCCGTCCACCGTTGCAGA
ACTGCGGCAATCCATCCAGGCGCTCTCGCCCGAATTCGTCGAAATCCGCCGGAAGATTCACGCCCATCCCGAACTCGCCT
TCGAAGAGCGCCAGACCAGCGACCTGGTCGCGGAACGCCTCGCGGCATGGGGCTACAAGATCCATCGTGGCCTGGGCATG
ACCGGCGTCGTCGGCACGCTGAGGAAGGGACACGGGACCAGGTCGCTGGGCATCCGCGCCGACATGGACGCGCTGCCGAT
CCAGGAAAAGACGGGCCTCGACTACGCGAGCACCATTCCCGGCAAGATGCACGCGTGCGGCCACGACGGCCACACCGCCA
TCCTGCTCTGCGCCGCGCGCCATCTCGCCCAATCCGTCGATTTCAACGGCACGCTGAACCTGATCTTCCAGCCCGCCGAA
GAAAACGAAGGCGGCGCGCTGCGCATGCTGGAAGACGGCCTGTTCGAGCGTTTCCCGTGCGACGAAGTCTATGCGCTGCA
CAACTCGCCGGGGCTGCCTGTGGGCCAGATCGGCGTGATCACCGGACCGGCCATGGCCTCGTTCGACCGCGCCACCGTCA
CGCTGCGCGGCCGCGGTGCGCACGGCGCCATGCCACACCACGGCATTGATCCGATGCAGTGCGCCGCCAGCATCGTGCTG
GGCCTGCAATCGATCGTCAGCCGCGAAATCGACGCGCTCAAGTCCGCAGTGATCACGGTCGGTTCGATCCAGGCCGGCGC
AACCTATAACGTCGTCCCCGAAAGCGCGCTGATCAAGATCGGCGTGCGCACGCTTGACCCGAAGGTACGCACGCTGGTCG
AGACGCGCATCCAGGCATTCGTCGCCGCGCAGGCCGAGAGCTATGGCCTGCAATCGGAAGTCGTGTACGAACGCAAGTAC
CCGGTGCTCGTCAACCATGCAGCGCAGACCGAACGCGCACGGGAAGCGGCAATTCGCCTGCTCGGCGAGGACAACGTCGT
GGAACGCCCGCCCGTGATGGGCAGCGAAGACTTCGCCTACATGCTCGAACAGCGGCCCGGCGCATACGTGCGCCTTGGCA
ATGGGCTGGGCGAAGACGGGGGTTGCATGGTCCATAACCCCCTGTACGACTTCAACGACAAAGCCTTGCCTGTCGGCGCC
GCATTCTGGGCGCACCTCGCGCAAAGCTACCTGGTTTGA

Upstream 100 bases:

>100_bases
TTCCGGTCCAGTACATCGTCCATCATTCCTCCCCCGATCCGCGCAACGTGGCGAACTTCAATCGTTGCATTTTATACAAC
GTTTTACCGCAATCATTGTC

Downstream 100 bases:

>100_bases
ATAAGCGACCTGAATCGACCTGAAACGGCCTGAATAATCCAAAAACGGGGACACCATGCATTCGATGAACCGCCGCCGCT
TCCTTCAATATGCCGGCTTC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MDAGRVRVENSSTIQPRHAMNPSTVAELRQSIQALSPEFVEIRRKIHAHPELAFEERQTSDLVAERLAAWGYKIHRGLGM
TGVVGTLRKGHGTRSLGIRADMDALPIQEKTGLDYASTIPGKMHACGHDGHTAILLCAARHLAQSVDFNGTLNLIFQPAE
ENEGGALRMLEDGLFERFPCDEVYALHNSPGLPVGQIGVITGPAMASFDRATVTLRGRGAHGAMPHHGIDPMQCAASIVL
GLQSIVSREIDALKSAVITVGSIQAGATYNVVPESALIKIGVRTLDPKVRTLVETRIQAFVAAQAESYGLQSEVVYERKY
PVLVNHAAQTERAREAAIRLLGEDNVVERPPVMGSEDFAYMLEQRPGAYVRLGNGLGEDGGCMVHNPLYDFNDKALPVGA
AFWAHLAQSYLV

Sequences:

>Translated_412_residues
MDAGRVRVENSSTIQPRHAMNPSTVAELRQSIQALSPEFVEIRRKIHAHPELAFEERQTSDLVAERLAAWGYKIHRGLGM
TGVVGTLRKGHGTRSLGIRADMDALPIQEKTGLDYASTIPGKMHACGHDGHTAILLCAARHLAQSVDFNGTLNLIFQPAE
ENEGGALRMLEDGLFERFPCDEVYALHNSPGLPVGQIGVITGPAMASFDRATVTLRGRGAHGAMPHHGIDPMQCAASIVL
GLQSIVSREIDALKSAVITVGSIQAGATYNVVPESALIKIGVRTLDPKVRTLVETRIQAFVAAQAESYGLQSEVVYERKY
PVLVNHAAQTERAREAAIRLLGEDNVVERPPVMGSEDFAYMLEQRPGAYVRLGNGLGEDGGCMVHNPLYDFNDKALPVGA
AFWAHLAQSYLV
>Mature_412_residues
MDAGRVRVENSSTIQPRHAMNPSTVAELRQSIQALSPEFVEIRRKIHAHPELAFEERQTSDLVAERLAAWGYKIHRGLGM
TGVVGTLRKGHGTRSLGIRADMDALPIQEKTGLDYASTIPGKMHACGHDGHTAILLCAARHLAQSVDFNGTLNLIFQPAE
ENEGGALRMLEDGLFERFPCDEVYALHNSPGLPVGQIGVITGPAMASFDRATVTLRGRGAHGAMPHHGIDPMQCAASIVL
GLQSIVSREIDALKSAVITVGSIQAGATYNVVPESALIKIGVRTLDPKVRTLVETRIQAFVAAQAESYGLQSEVVYERKY
PVLVNHAAQTERAREAAIRLLGEDNVVERPPVMGSEDFAYMLEQRPGAYVRLGNGLGEDGGCMVHNPLYDFNDKALPVGA
AFWAHLAQSYLV

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=29.7423887587822, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 44622; Mature: 44622

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAGRVRVENSSTIQPRHAMNPSTVAELRQSIQALSPEFVEIRRKIHAHPELAFEERQTS
CCCCCEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
DLVAERLAAWGYKIHRGLGMTGVVGTLRKGHGTRSLGIRADMDALPIQEKTGLDYASTIP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCHHCCCCHHHCCC
GKMHACGHDGHTAILLCAARHLAQSVDFNGTLNLIFQPAEENEGGALRMLEDGLFERFPC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHCCC
DEVYALHNSPGLPVGQIGVITGPAMASFDRATVTLRGRGAHGAMPHHGIDPMQCAASIVL
CHHEEEECCCCCCCCCEEEEECCCHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GLQSIVSREIDALKSAVITVGSIQAGATYNVVPESALIKIGVRTLDPKVRTLVETRIQAF
HHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHH
VAAQAESYGLQSEVVYERKYPVLVNHAAQTERAREAAIRLLGEDNVVERPPVMGSEDFAY
HHHHHHHCCCCHHEEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
MLEQRPGAYVRLGNGLGEDGGCMVHNPLYDFNDKALPVGAAFWAHLAQSYLV
HHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAGRVRVENSSTIQPRHAMNPSTVAELRQSIQALSPEFVEIRRKIHAHPELAFEERQTS
CCCCCEEECCCCCCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
DLVAERLAAWGYKIHRGLGMTGVVGTLRKGHGTRSLGIRADMDALPIQEKTGLDYASTIP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCHHCCCCHHHCCC
GKMHACGHDGHTAILLCAARHLAQSVDFNGTLNLIFQPAEENEGGALRMLEDGLFERFPC
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEHHHHHHHHCCC
DEVYALHNSPGLPVGQIGVITGPAMASFDRATVTLRGRGAHGAMPHHGIDPMQCAASIVL
CHHEEEECCCCCCCCCEEEEECCCHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GLQSIVSREIDALKSAVITVGSIQAGATYNVVPESALIKIGVRTLDPKVRTLVETRIQAF
HHHHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHH
VAAQAESYGLQSEVVYERKYPVLVNHAAQTERAREAAIRLLGEDNVVERPPVMGSEDFAY
HHHHHHHCCCCHHEEEECCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
MLEQRPGAYVRLGNGLGEDGGCMVHNPLYDFNDKALPVGAAFWAHLAQSYLV
HHHCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]