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The map label for this gene is shiA [H]

Identifier: 73537619

GI number: 73537619

Start: 385027

End: 386628

Strand: Reverse

Name: shiA [H]

Synonym: Reut_B3784

Alternate gene names: 73537619

Gene position: 386628-385027 (Counterclockwise)

Preceding gene: 73537628

Following gene: 73537618

Centisome position: 14.18

GC content: 63.3

Gene sequence:

>1602_bases
ATGACTATGAGCCTGACCCGCGATGAAGCGCGCGCCTCCGCTTCCGGCATGACCGCCGAAGAACGCAAGGTCATCCTTGC
CTCCTCCTTCGGTGCGCTGATGGAGTGGTACGACTTTTACATCTACGCTGCGCTGGCGGTTTACTTCGGCGCCCTGTTCT
TCCCGAAGGGCAATGAAACCGCCGCCTTCCTCGCCAGCCTCGCGACGTTCGGCGCCGGCTTCCTGATCCGCCCCGTTGGC
GCACTGCTGTTCGGTCGTCTGGGCGACCTAATCGGCCGCAAGTACACCTTTCTCGTCACCATCCTGCTGATGGGCATTGC
CACGGTCGGCGTTGGCGTCCTGCCCACCTACGAGCAGATCGGCATCGCAGCCACCGTGCTGCTGGTGCTGCTGCGGTTGT
TGCAGGGCCTCGCGCTCGGCGGCGAGGTGGGCGGCGCGGTCACCTACGTGGCCGAGCATTCGCCGGTGAGCAAGCGCGGC
CTGTACACAAGTTCGCTGCAGACCACGGCGACCCTGGGCCTGCTGTCGTCGCTGTTCGTGGTGTATCTGCTCAAGACGTT
CCTGACCGAGGCGGAATTCCGTGCCTGGGGCTGGCGCATTCCGTTCATCGTGTCGCTGGTGATGCTGGTGATATCGGTGT
ACATCCGCGGCAAGCTGCACGAATCGCCAGTGTTTGCGCGCATGAAGGCCGGCAACGCGACATCGAAGGCGCCGATCCGC
GAGAGCTTCACGCAATGGCACAACCTCAAGTTCGTGCTGCTGCTGTTCGTGGTGGCAGCCGGCCTTGGTGCGATTTTCGG
CACGGGCCACTTCTACACGATGTTCTTCGTGAACAAGACGCTGCATGTGCCGATCGAGACGGTGCACATGCTGATCGCCA
TCGCACTGGTTGTAGCCACGCCTTGTTACCTGTTCTTCGGCTGGCTGTCGGATCGCATCGGCCGCAAGTACATCATGATG
GTGGCCTGCTTGCTGGCGGCGCTCACGATTCAGCCCATCTTCCAGGGCCTGACGCACTACGCCAATCCGGCGCTCGAACA
CTTCCAGAAGCAGGGAAGCGTGCAGGTCACTGCAGGCAACTGCAAAGCGCGTCTGTTCGGTGATCCGGTGTCCGACTGCG
ACAAGATCCGCGGCTACCTGACCGACCTTGGCGTGAGCTACACCTTCGTGCCCGCCAGCGATGCGAAGGCCGAAATCCGC
ATCGGCGCGCAAGCGCTGCAGGGCTTTGACCGCGACGCAATCAAGCAAGCGCTCGTCGCAGCCGGCTGGCCCGAGCGCGC
CGATCCGGCGCACATCAATATGCCGATGCTGTTCCTGCTGCTGCTCGTGCCCATCCTGTACCTCGCCATGGTGTACGGGC
CGATGGCCGCGTTCATGGTCGAACTGTTCCCGGCACGCGTGCGCTACACCTCGCTGTCCCTGCCCTTCCACCTGGGTGCG
GGCTGGGTCGGCGGCATGCTGTCCTTCGTCGTCACGGCGATGAATGTATCGAGCGGCGACGTCTACTACGGGCTTTGGTA
TCCGGTGATCATCGCGGGCGCTGCATTCGTGATCGGCATGCTGTTCGTGCCGGAGACGCGCGGTCGCGACCTGTCCACCT
GA

Upstream 100 bases:

>100_bases
CGCAACCGGGCTGTCGGCAGCCAAGTGGCTCGCTGCTTTGCACCATCCGGGAAGAAGAAAAGACAAATGAGACAACTGGC
TTCACCATAGGACCCCCGCC

Downstream 100 bases:

>100_bases
ACCGCACGCGGACCCGTGAAGGGGCGGAGTCATCCGCCCTCTTTTCCGCCACATCGTCAGCATACTGATTTCATGACCGA
GTGTCCCGGCGCGGCGATGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTMSLTRDEARASASGMTAEERKVILASSFGALMEWYDFYIYAALAVYFGALFFPKGNETAAFLASLATFGAGFLIRPVG
ALLFGRLGDLIGRKYTFLVTILLMGIATVGVGVLPTYEQIGIAATVLLVLLRLLQGLALGGEVGGAVTYVAEHSPVSKRG
LYTSSLQTTATLGLLSSLFVVYLLKTFLTEAEFRAWGWRIPFIVSLVMLVISVYIRGKLHESPVFARMKAGNATSKAPIR
ESFTQWHNLKFVLLLFVVAAGLGAIFGTGHFYTMFFVNKTLHVPIETVHMLIAIALVVATPCYLFFGWLSDRIGRKYIMM
VACLLAALTIQPIFQGLTHYANPALEHFQKQGSVQVTAGNCKARLFGDPVSDCDKIRGYLTDLGVSYTFVPASDAKAEIR
IGAQALQGFDRDAIKQALVAAGWPERADPAHINMPMLFLLLLVPILYLAMVYGPMAAFMVELFPARVRYTSLSLPFHLGA
GWVGGMLSFVVTAMNVSSGDVYYGLWYPVIIAGAAFVIGMLFVPETRGRDLST

Sequences:

>Translated_533_residues
MTMSLTRDEARASASGMTAEERKVILASSFGALMEWYDFYIYAALAVYFGALFFPKGNETAAFLASLATFGAGFLIRPVG
ALLFGRLGDLIGRKYTFLVTILLMGIATVGVGVLPTYEQIGIAATVLLVLLRLLQGLALGGEVGGAVTYVAEHSPVSKRG
LYTSSLQTTATLGLLSSLFVVYLLKTFLTEAEFRAWGWRIPFIVSLVMLVISVYIRGKLHESPVFARMKAGNATSKAPIR
ESFTQWHNLKFVLLLFVVAAGLGAIFGTGHFYTMFFVNKTLHVPIETVHMLIAIALVVATPCYLFFGWLSDRIGRKYIMM
VACLLAALTIQPIFQGLTHYANPALEHFQKQGSVQVTAGNCKARLFGDPVSDCDKIRGYLTDLGVSYTFVPASDAKAEIR
IGAQALQGFDRDAIKQALVAAGWPERADPAHINMPMLFLLLLVPILYLAMVYGPMAAFMVELFPARVRYTSLSLPFHLGA
GWVGGMLSFVVTAMNVSSGDVYYGLWYPVIIAGAAFVIGMLFVPETRGRDLST
>Mature_532_residues
TMSLTRDEARASASGMTAEERKVILASSFGALMEWYDFYIYAALAVYFGALFFPKGNETAAFLASLATFGAGFLIRPVGA
LLFGRLGDLIGRKYTFLVTILLMGIATVGVGVLPTYEQIGIAATVLLVLLRLLQGLALGGEVGGAVTYVAEHSPVSKRGL
YTSSLQTTATLGLLSSLFVVYLLKTFLTEAEFRAWGWRIPFIVSLVMLVISVYIRGKLHESPVFARMKAGNATSKAPIRE
SFTQWHNLKFVLLLFVVAAGLGAIFGTGHFYTMFFVNKTLHVPIETVHMLIAIALVVATPCYLFFGWLSDRIGRKYIMMV
ACLLAALTIQPIFQGLTHYANPALEHFQKQGSVQVTAGNCKARLFGDPVSDCDKIRGYLTDLGVSYTFVPASDAKAEIRI
GAQALQGFDRDAIKQALVAAGWPERADPAHINMPMLFLLLLVPILYLAMVYGPMAAFMVELFPARVRYTSLSLPFHLGAG
WVGGMLSFVVTAMNVSSGDVYYGLWYPVIIAGAAFVIGMLFVPETRGRDLST

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=344, Percent_Identity=35.4651162790698, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1790550, Length=315, Percent_Identity=36.8253968253968, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789941, Length=334, Percent_Identity=39.8203592814371, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788942, Length=322, Percent_Identity=35.0931677018634, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=24.5535714285714, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082404, Length=332, Percent_Identity=24.0963855421687, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58060; Mature: 57929

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSLTRDEARASASGMTAEERKVILASSFGALMEWYDFYIYAALAVYFGALFFPKGNET
CCCCCCHHHHHHHHCCCCHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AAFLASLATFGAGFLIRPVGALLFGRLGDLIGRKYTFLVTILLMGIATVGVGVLPTYEQI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
GIAATVLLVLLRLLQGLALGGEVGGAVTYVAEHSPVSKRGLYTSSLQTTATLGLLSSLFV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHH
VYLLKTFLTEAEFRAWGWRIPFIVSLVMLVISVYIRGKLHESPVFARMKAGNATSKAPIR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCHHH
ESFTQWHNLKFVLLLFVVAAGLGAIFGTGHFYTMFFVNKTLHVPIETVHMLIAIALVVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCEEECCHHHHHHHHHHHHHHHH
PCYLFFGWLSDRIGRKYIMMVACLLAALTIQPIFQGLTHYANPALEHFQKQGSVQVTAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECC
CKARLFGDPVSDCDKIRGYLTDLGVSYTFVPASDAKAEIRIGAQALQGFDRDAIKQALVA
CEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHEECHHHHCCCCHHHHHHHHHH
AGWPERADPAHINMPMLFLLLLVPILYLAMVYGPMAAFMVELFPARVRYTSLSLPFHLGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GWVGGMLSFVVTAMNVSSGDVYYGLWYPVIIAGAAFVIGMLFVPETRGRDLST
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TMSLTRDEARASASGMTAEERKVILASSFGALMEWYDFYIYAALAVYFGALFFPKGNET
CCCCCHHHHHHHHCCCCHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AAFLASLATFGAGFLIRPVGALLFGRLGDLIGRKYTFLVTILLMGIATVGVGVLPTYEQI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
GIAATVLLVLLRLLQGLALGGEVGGAVTYVAEHSPVSKRGLYTSSLQTTATLGLLSSLFV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHH
VYLLKTFLTEAEFRAWGWRIPFIVSLVMLVISVYIRGKLHESPVFARMKAGNATSKAPIR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCHHH
ESFTQWHNLKFVLLLFVVAAGLGAIFGTGHFYTMFFVNKTLHVPIETVHMLIAIALVVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCEEECCHHHHHHHHHHHHHHHH
PCYLFFGWLSDRIGRKYIMMVACLLAALTIQPIFQGLTHYANPALEHFQKQGSVQVTAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECC
CKARLFGDPVSDCDKIRGYLTDLGVSYTFVPASDAKAEIRIGAQALQGFDRDAIKQALVA
CEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHEECHHHHCCCCHHHHHHHHHH
AGWPERADPAHINMPMLFLLLLVPILYLAMVYGPMAAFMVELFPARVRYTSLSLPFHLGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
GWVGGMLSFVVTAMNVSSGDVYYGLWYPVIIAGAAFVIGMLFVPETRGRDLST
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]