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The map label for this gene is paaX [H]

Identifier: 73537569

GI number: 73537569

Start: 327765

End: 328688

Strand: Reverse

Name: paaX [H]

Synonym: Reut_B3734

Alternate gene names: 73537569

Gene position: 328688-327765 (Counterclockwise)

Preceding gene: 73537580

Following gene: 73537568

Centisome position: 12.06

GC content: 67.53

Gene sequence:

>924_bases
ATGGCCACTCGTTCGGCGACACAACCGGTTTCCCCGCAGGTCGCGCGGCTCGCACGCGGCCTTAAGCTCGGCGCCAATTC
GATGCTCGTGACACTGTTTGGCGATGTGGTCGCGCCGCGGCCTCAGGCGCTGTGGCTGGGCAGCCTGATCCGCCTGGCCG
AGCCGTTCGGCATCAACGACCGGCTTGTACGCACTGCGACGTTCCGGCTGACGTCCGATGACTGGCTCAACGCCACGCGC
ATCGGGCGGCGCAGCTACTACGGCTTGTCCGAGGCGGGGCTGCAGCGCTGCCTGCATGCCGGCAAGCGCATCTACGCCGG
CGACGCACCCGACTGGGACGGCCGCTGGACGTTGGCGCTGGTGCGTGGCGACGCGCGCGCCACCATCCGCCAGCGATTGA
AGCGCGAGCTGCTGTGGGAAGGCTTCGGCGCGATCGCGCCGGGCGTGTATGCGCATCCGAATGCCGATGCAAACTCGCTA
GGCGAGATCATCCGTGCAGCGCATGCGCAGGACTTCGTCGCGGTGATGGACGCGACCAGCCTCGAGACATTCTCGATCCG
ACCGCTGCAGACGTTGATGCACCAGACGTTCAAGCTCGGCGACGTGGCGTCCGCGTGGCAGGCGCTGCTGCGCCGCTTCT
CGCCCGTGCTGGCCGACGCACATGCCATGACGCCGGCCGACGCCTTTTTCGTACGCACGCTGCTGCTGCACGAATACCGC
CGCGTGCTGCTGCGCGACCCGAACCTGCCGGAACAACTGCTGCCCACGGACTGGCCCGGTCGCACTGCGCGAGACCTGTG
CCGTGATATGTACGCGGCACTGCTGGATGCCAGCGAGGACTATCTGCGCGAGGTTGTGGAGGTATCCGAAGGTACGCTGG
CCAACGCCACCCGGCTTCTGCGCAGGCGCTTTGCCATGGCGTAG

Upstream 100 bases:

>100_bases
CTGCGTCAAATCATTCGGTTGCAATGGATGATAGTGATACAATTTGATTCAAACAATCGACGTTTGTGTATCACTTTCGG
AATCATTTTGAGGGGCATGC

Downstream 100 bases:

>100_bases
GCGGTTTGTGCCTGCTCATACCGCGGCCGCACCGGGCCCGGTCCGACGAGCTAGCATTTCGAAAGACGCAATCAACTAGG
CATTTCCGAAATTCCGTCCT

Product: phenylacetic acid degradation operon negative regulatory protein PaaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MATRSATQPVSPQVARLARGLKLGANSMLVTLFGDVVAPRPQALWLGSLIRLAEPFGINDRLVRTATFRLTSDDWLNATR
IGRRSYYGLSEAGLQRCLHAGKRIYAGDAPDWDGRWTLALVRGDARATIRQRLKRELLWEGFGAIAPGVYAHPNADANSL
GEIIRAAHAQDFVAVMDATSLETFSIRPLQTLMHQTFKLGDVASAWQALLRRFSPVLADAHAMTPADAFFVRTLLLHEYR
RVLLRDPNLPEQLLPTDWPGRTARDLCRDMYAALLDASEDYLREVVEVSEGTLANATRLLRRRFAMA

Sequences:

>Translated_307_residues
MATRSATQPVSPQVARLARGLKLGANSMLVTLFGDVVAPRPQALWLGSLIRLAEPFGINDRLVRTATFRLTSDDWLNATR
IGRRSYYGLSEAGLQRCLHAGKRIYAGDAPDWDGRWTLALVRGDARATIRQRLKRELLWEGFGAIAPGVYAHPNADANSL
GEIIRAAHAQDFVAVMDATSLETFSIRPLQTLMHQTFKLGDVASAWQALLRRFSPVLADAHAMTPADAFFVRTLLLHEYR
RVLLRDPNLPEQLLPTDWPGRTARDLCRDMYAALLDASEDYLREVVEVSEGTLANATRLLRRRFAMA
>Mature_306_residues
ATRSATQPVSPQVARLARGLKLGANSMLVTLFGDVVAPRPQALWLGSLIRLAEPFGINDRLVRTATFRLTSDDWLNATRI
GRRSYYGLSEAGLQRCLHAGKRIYAGDAPDWDGRWTLALVRGDARATIRQRLKRELLWEGFGAIAPGVYAHPNADANSLG
EIIRAAHAQDFVAVMDATSLETFSIRPLQTLMHQTFKLGDVASAWQALLRRFSPVLADAHAMTPADAFFVRTLLLHEYRR
VLLRDPNLPEQLLPTDWPGRTARDLCRDMYAALLDASEDYLREVVEVSEGTLANATRLLRRRFAMA

Specific function: Negative regulator of the paaZ and paaABCDEFGHIJK catabolic operons. Binds the consensus sequence 5'- WWTRTGATTCGYGWT-3'. Binding of paaX is specifically inhibited by phenylacetyl-coenzyme A [H]

COG id: COG3327

COG function: function code K; Phenylacetic acid-responsive transcriptional repressor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787666, Length=287, Percent_Identity=36.2369337979094, Blast_Score=195, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012906
- InterPro:   IPR013225
- InterPro:   IPR011965 [H]

Pfam domain/function: PF07848 PaaX; PF08223 PaaX_C [H]

EC number: NA

Molecular weight: Translated: 34248; Mature: 34117

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRSATQPVSPQVARLARGLKLGANSMLVTLFGDVVAPRPQALWLGSLIRLAEPFGIND
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
RLVRTATFRLTSDDWLNATRIGRRSYYGLSEAGLQRCLHAGKRIYAGDAPDWDGRWTLAL
HHHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEEE
VRGDARATIRQRLKRELLWEGFGAIAPGVYAHPNADANSLGEIIRAAHAQDFVAVMDATS
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LETFSIRPLQTLMHQTFKLGDVASAWQALLRRFSPVLADAHAMTPADAFFVRTLLLHEYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RVLLRDPNLPEQLLPTDWPGRTARDLCRDMYAALLDASEDYLREVVEVSEGTLANATRLL
HHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
RRRFAMA
HHHHCCC
>Mature Secondary Structure 
ATRSATQPVSPQVARLARGLKLGANSMLVTLFGDVVAPRPQALWLGSLIRLAEPFGIND
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
RLVRTATFRLTSDDWLNATRIGRRSYYGLSEAGLQRCLHAGKRIYAGDAPDWDGRWTLAL
HHHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEEE
VRGDARATIRQRLKRELLWEGFGAIAPGVYAHPNADANSLGEIIRAAHAQDFVAVMDATS
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LETFSIRPLQTLMHQTFKLGDVASAWQALLRRFSPVLADAHAMTPADAFFVRTLLLHEYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RVLLRDPNLPEQLLPTDWPGRTARDLCRDMYAALLDASEDYLREVVEVSEGTLANATRLL
HHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
RRRFAMA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]