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The map label for this gene is lutR [H]

Identifier: 73537549

GI number: 73537549

Start: 302791

End: 303534

Strand: Reverse

Name: lutR [H]

Synonym: Reut_B3714

Alternate gene names: 73537549

Gene position: 303534-302791 (Counterclockwise)

Preceding gene: 73537552

Following gene: 73537548

Centisome position: 11.13

GC content: 68.68

Gene sequence:

>744_bases
ATGACCATGCCCGACGCCCAGATGACTTCGCCCGCACCGCTGCGCCGCCGCGGCCGTACGCTTGCCGAAGAAGTGGTCAG
CGGCCTGACCGAGCGCATTCGCCAGGGACAGCTGAAGCCGGGCGACAAGCTGCCCACCGAATCCGAGGTCATGGCCACGT
TTGGCGTGAGCCGCACCGTCGTGCGCGAGGCGCTGTCGCGCCTGCAGGCCAGCGGCCTGGTGGAAACGCGTCACGGGATT
GGCACGTTCGTGCTCGCGCGCCCTGCCGAAACGCCGTCACCATTCCGCATCGACACCGCCGCCATGGGCACCGCCATGGA
TGTGCTGGCCATGCTGGAGTTTCGCGTGAGCCTGGAAGCGGAAGCCGCCGGCCTGGCGGCGACGCGCCGCGGCGACGAAC
ATCTGGCCGCCATGCGCCGTGCGCTCGACTCCATGAAGCGCAATACGACCGAAGGCAGCGACGCCGTCGGCGCCGATTTC
GAGTTCCACCTGTCCATCGCGCGCGCCACTGGCAACCGCTACTTCACCGACATCATGGGCCACCTGGGCACCATGCTGAT
CCCGCGCAGCCGCCTGCAGGTCAATACGCAGGAGCGCGCCCAGTATCTGGAACGCGTGCATGTCGAGCACGAGAACATCT
ACGACGCCATCGAGCGCCGCGACCCGGAGGCCGCCAAGGCCGCCATGCGCATGCACCTGACCAACAGCCGCGAGCGCCTG
CGCAAGGCGTCCGCGGCAGCCTGA

Upstream 100 bases:

>100_bases
AATGCTTTAGCATACGTTATCAGTCGTCGTATGACTTACAACCGAGTATAATGACGGCATCCCGGCCAGGTCAAGGCCGC
AATTTCCCTACGCCATTCCC

Downstream 100 bases:

>100_bases
GCCTGCGTCGTCCCTACTGTTGCGGACGACCGTCGCATGTCATATGATGTCCTATGAGATTCGGCAGCATGCTCAGGCCG
ATCATTCCGTTTGATGGTGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTMPDAQMTSPAPLRRRGRTLAEEVVSGLTERIRQGQLKPGDKLPTESEVMATFGVSRTVVREALSRLQASGLVETRHGI
GTFVLARPAETPSPFRIDTAAMGTAMDVLAMLEFRVSLEAEAAGLAATRRGDEHLAAMRRALDSMKRNTTEGSDAVGADF
EFHLSIARATGNRYFTDIMGHLGTMLIPRSRLQVNTQERAQYLERVHVEHENIYDAIERRDPEAAKAAMRMHLTNSRERL
RKASAAA

Sequences:

>Translated_247_residues
MTMPDAQMTSPAPLRRRGRTLAEEVVSGLTERIRQGQLKPGDKLPTESEVMATFGVSRTVVREALSRLQASGLVETRHGI
GTFVLARPAETPSPFRIDTAAMGTAMDVLAMLEFRVSLEAEAAGLAATRRGDEHLAAMRRALDSMKRNTTEGSDAVGADF
EFHLSIARATGNRYFTDIMGHLGTMLIPRSRLQVNTQERAQYLERVHVEHENIYDAIERRDPEAAKAAMRMHLTNSRERL
RKASAAA
>Mature_246_residues
TMPDAQMTSPAPLRRRGRTLAEEVVSGLTERIRQGQLKPGDKLPTESEVMATFGVSRTVVREALSRLQASGLVETRHGIG
TFVLARPAETPSPFRIDTAAMGTAMDVLAMLEFRVSLEAEAAGLAATRRGDEHLAAMRRALDSMKRNTTEGSDAVGADFE
FHLSIARATGNRYFTDIMGHLGTMLIPRSRLQVNTQERAQYLERVHVEHENIYDAIERRDPEAAKAAMRMHLTNSRERLR
KASAAA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=233, Percent_Identity=32.618025751073, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=29.2035398230088, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789621, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789353, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994955, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790032, Length=231, Percent_Identity=31.1688311688312, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786303, Length=227, Percent_Identity=29.9559471365639, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786950, Length=66, Percent_Identity=43.9393939393939, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27300; Mature: 27168

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPDAQMTSPAPLRRRGRTLAEEVVSGLTERIRQGQLKPGDKLPTESEVMATFGVSRTV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
VREALSRLQASGLVETRHGIGTFVLARPAETPSPFRIDTAAMGTAMDVLAMLEFRVSLEA
HHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
EAAGLAATRRGDEHLAAMRRALDSMKRNTTEGSDAVGADFEFHLSIARATGNRYFTDIMG
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHCCCHHHHHHHH
HLGTMLIPRSRLQVNTQERAQYLERVHVEHENIYDAIERRDPEAAKAAMRMHLTNSRERL
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKASAAA
HHHHCCC
>Mature Secondary Structure 
TMPDAQMTSPAPLRRRGRTLAEEVVSGLTERIRQGQLKPGDKLPTESEVMATFGVSRTV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
VREALSRLQASGLVETRHGIGTFVLARPAETPSPFRIDTAAMGTAMDVLAMLEFRVSLEA
HHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHH
EAAGLAATRRGDEHLAAMRRALDSMKRNTTEGSDAVGADFEFHLSIARATGNRYFTDIMG
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHCCCHHHHHHHH
HLGTMLIPRSRLQVNTQERAQYLERVHVEHENIYDAIERRDPEAAKAAMRMHLTNSRERL
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RKASAAA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]