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The map label for this gene is bcsZ [H]

Identifier: 73537361

GI number: 73537361

Start: 70634

End: 71812

Strand: Reverse

Name: bcsZ [H]

Synonym: Reut_B3526

Alternate gene names: 73537361

Gene position: 71812-70634 (Counterclockwise)

Preceding gene: 161611273

Following gene: 73537360

Centisome position: 2.63

GC content: 68.62

Gene sequence:

>1179_bases
ATGTGGCGCGCCTTGCCGGGTCTGACCAGGTGGCTCGCGGCCGGCGCGCTCGCGTGTGCCTGCGTCGCCGCGTTGCCCGC
GCATGCCGCCACATGCGGCTGGGCCGACTGGGACACCTTCAAACGCACGCTGATCAGCGCCGACGGGCGCGTCATCGACG
CGAGCACAGACGACAAGGTCACCGTGTCTGAAGGGCAGGCCTATGGCCTGTTCTTCGCGCTTGTGGCGAACGACCGCACC
ACGTTCGACAAGCTCCTCGCGTGGACCGAGAACAACCTTGCGCAGGGCGATCTCATCGCCCATCTGCCCGCCTGGATCTG
GGGCCGTATTCCCGCCGACAAGGCCAAGGACGGCAAGGAGACGTGGGGCGTGATCGACACCAACTCCGCTTCCGACGCCG
ACCTGTGGATCGCCTACACGCTGCTGGAAGCGGGCCGCCTGTGGAACGAGCGGCGCTTCACCGCGCTCGGCACGCTGACG
GCGCGCCGCATCATGCGCGAAGAGACTGCGGCCATGCGCGGCCTGGGCCGCACCGTGCTGCCTGGCCCGGTCGGCTTTTC
GCTGAGTGAAGGCCGCTGGCGCGTAAATCCGAGCTATGTGCCGCTGCAGGTCATGCGGCGCCTTGCTGTGTTGCTGGCAA
AAGAGTCTGGCTGGGACCAGCTTGTGGCCAGCTCGTTGAAGGTCATCCTGGAAACCGCGCCGCGCGGCTTCTCGCCGGAC
TGGGCCGAGTACGACAACAGCCGCGGCTTTCTGCCCGATACCGCAACCAAGGCCGAAAGCGCCTACAACGCGATCCGCGT
CTATCTGTGGGCGGGCACGCTTGCCGCCGACGATCCGCAGCGAGCCCGCATGCTGCAGCAGTTCATGCCGCTGGCAGACT
ACGTCGCAGCCCACGGATATGCGCCTGAACGCGTCGACACGCAGACCGGTCAGCCAGGCCCCGATTCCGGCAATGCCGGT
TTCTCGGCGGCGGTCGCGCCCTACCTGGCCGCGCTCGGCCGCACCGACCAGGCGCGCGCGCAGGCGCAGCGCACGCGCGA
ACTGACTGCACGCGAGCCGCTCGGCTACTACAGCCAGGCGCTTGCGCTGTTCGGCCTCGGCCACCTGGACGGCCTGTACC
GCTTTGCAGGGGACGGCGCCCTGCTGCCAGCCTGGAAGACCGGATGTCCCGCGCGCTGA

Upstream 100 bases:

>100_bases
AGCCTGATGGCCATTGCCGGCCTGCTCGCCGGGCTGGTCGTGGCGCTGTCGCTGTTCTGGACGCTGGGCAAGCTTGCGGC
ACGGCGCAACGGAGGCTGAC

Downstream 100 bases:

>100_bases
CGCCCCTCGTACTCACCGTCGTCAGCGCGCTGGCGGTGCCCGCCATGGCGCAGCAGGCAGAGAAGCCGTCGCCGGAGATG
ACGCAGTTGCTGGCCGCTGC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MWRALPGLTRWLAAGALACACVAALPAHAATCGWADWDTFKRTLISADGRVIDASTDDKVTVSEGQAYGLFFALVANDRT
TFDKLLAWTENNLAQGDLIAHLPAWIWGRIPADKAKDGKETWGVIDTNSASDADLWIAYTLLEAGRLWNERRFTALGTLT
ARRIMREETAAMRGLGRTVLPGPVGFSLSEGRWRVNPSYVPLQVMRRLAVLLAKESGWDQLVASSLKVILETAPRGFSPD
WAEYDNSRGFLPDTATKAESAYNAIRVYLWAGTLAADDPQRARMLQQFMPLADYVAAHGYAPERVDTQTGQPGPDSGNAG
FSAAVAPYLAALGRTDQARAQAQRTRELTAREPLGYYSQALALFGLGHLDGLYRFAGDGALLPAWKTGCPAR

Sequences:

>Translated_392_residues
MWRALPGLTRWLAAGALACACVAALPAHAATCGWADWDTFKRTLISADGRVIDASTDDKVTVSEGQAYGLFFALVANDRT
TFDKLLAWTENNLAQGDLIAHLPAWIWGRIPADKAKDGKETWGVIDTNSASDADLWIAYTLLEAGRLWNERRFTALGTLT
ARRIMREETAAMRGLGRTVLPGPVGFSLSEGRWRVNPSYVPLQVMRRLAVLLAKESGWDQLVASSLKVILETAPRGFSPD
WAEYDNSRGFLPDTATKAESAYNAIRVYLWAGTLAADDPQRARMLQQFMPLADYVAAHGYAPERVDTQTGQPGPDSGNAG
FSAAVAPYLAALGRTDQARAQAQRTRELTAREPLGYYSQALALFGLGHLDGLYRFAGDGALLPAWKTGCPAR
>Mature_392_residues
MWRALPGLTRWLAAGALACACVAALPAHAATCGWADWDTFKRTLISADGRVIDASTDDKVTVSEGQAYGLFFALVANDRT
TFDKLLAWTENNLAQGDLIAHLPAWIWGRIPADKAKDGKETWGVIDTNSASDADLWIAYTLLEAGRLWNERRFTALGTLT
ARRIMREETAAMRGLGRTVLPGPVGFSLSEGRWRVNPSYVPLQVMRRLAVLLAKESGWDQLVASSLKVILETAPRGFSPD
WAEYDNSRGFLPDTATKAESAYNAIRVYLWAGTLAADDPQRARMLQQFMPLADYVAAHGYAPERVDTQTGQPGPDSGNAG
FSAAVAPYLAALGRTDQARAQAQRTRELTAREPLGYYSQALALFGLGHLDGLYRFAGDGALLPAWKTGCPAR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=378, Percent_Identity=43.9153439153439, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 42679; Mature: 42679

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRALPGLTRWLAAGALACACVAALPAHAATCGWADWDTFKRTLISADGRVIDASTDDKV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCE
TVSEGQAYGLFFALVANDRTTFDKLLAWTENNLAQGDLIAHLPAWIWGRIPADKAKDGKE
EECCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
TWGVIDTNSASDADLWIAYTLLEAGRLWNERRFTALGTLTARRIMREETAAMRGLGRTVL
HCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGPVGFSLSEGRWRVNPSYVPLQVMRRLAVLLAKESGWDQLVASSLKVILETAPRGFSPD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
WAEYDNSRGFLPDTATKAESAYNAIRVYLWAGTLAADDPQRARMLQQFMPLADYVAAHGY
HHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
APERVDTQTGQPGPDSGNAGFSAAVAPYLAALGRTDQARAQAQRTRELTAREPLGYYSQA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LALFGLGHLDGLYRFAGDGALLPAWKTGCPAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWRALPGLTRWLAAGALACACVAALPAHAATCGWADWDTFKRTLISADGRVIDASTDDKV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCE
TVSEGQAYGLFFALVANDRTTFDKLLAWTENNLAQGDLIAHLPAWIWGRIPADKAKDGKE
EECCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
TWGVIDTNSASDADLWIAYTLLEAGRLWNERRFTALGTLTARRIMREETAAMRGLGRTVL
HCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PGPVGFSLSEGRWRVNPSYVPLQVMRRLAVLLAKESGWDQLVASSLKVILETAPRGFSPD
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
WAEYDNSRGFLPDTATKAESAYNAIRVYLWAGTLAADDPQRARMLQQFMPLADYVAAHGY
HHHHCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
APERVDTQTGQPGPDSGNAGFSAAVAPYLAALGRTDQARAQAQRTRELTAREPLGYYSQA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHH
LALFGLGHLDGLYRFAGDGALLPAWKTGCPAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]