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The map label for this gene is kefA [H]

Identifier: 73537317

GI number: 73537317

Start: 18591

End: 21032

Strand: Reverse

Name: kefA [H]

Synonym: Reut_B3482

Alternate gene names: 73537317

Gene position: 21032-18591 (Counterclockwise)

Preceding gene: 73537319

Following gene: 73537316

Centisome position: 0.77

GC content: 67.04

Gene sequence:

>2442_bases
ATGCGCAGACTTTCCTGGCTCGCCCTGAGCCTTGTGCTCACCGCACTGTTCCACCTGATGCCGGCCGCGCTGGCGGCCGA
GCCGGCGTCCGCGCCCGAAGCGGACCAGCCTCCGGCCCTCACCCACGCAGAGGCCATCACCGAACTCAAGCGCTTGCAGA
CCGAACAGGATCGCATCAAGCAGTCGGCATCAAGCGTCAACGGCACAACGCGCCTCGGTGACCTCGACGAAGGCCTGCAC
CAGCTCGCCAAGGACGTCGACAAGCTGACCGCCGCGCTGACGCCCCAGCGTGCGCAGTTGCAGGCCCAGCTCGACGTGCT
CGGGCCACCGCCCGCAGCGGGCGCCACGCCGCCGGAAATGCCCGCCGTGGCACGCCAGCGGGCCGAGCTCAATGCGCGCA
AGGTCCAGCTCGACAACGCGCTGAAACAGGCAGCAGAAGGCAAGGAGAACATCGAGAATCTGAACGCCCAGCTCGCCAAG
CTGCAGCGCAGCCGCATGAAGGACCAGCTTGCGCTGCGCTCGGAAAGCATCCTGAACCCGCAGTTCTGGGCGCCGCTGTT
CAAGCCATCCGATGACGACAGCGCCCGCTTGGAAGCGTTCGTGGATGAAGTTGTGCCGCTGTTGAAGGCCGCATGGGAAC
CGGAGCAGCGCGGCGCCACCGCGCTGCTGCTCGTACTGGCACTCGCGGTCTGGACGCTCGGCCGGCGACTTGCCGAACGT
GCCGCCGCCTGGTTCTGCCTGACGCGCTTGCCCGAAACCCGCTTGCGGCGCAGCGCGCTCGCGCTCGCCACCGCGCTTAC
CACCGTCGTCACGACGGCGCTCGCGGTGCAGCTGGTCTACTACGCCTTCACGCGCGGCTACGAGCCGCCGACTGACCTGC
AGGACCTGCTCGGCGCACTGGCCAGGCTCATGCTGACCAGTGCGCTGATTGCTGGGCTCGGCCGCGCGCTGCTGTGTACG
CATCACCCTTCGTGGCGACTGCCGGCGATGGCGGACCCGGTGGCGCGCGCACTCAAGCCGTTCCCGCGCGCACTGGCCGG
GCTGTTGCTGCTGGCGGGCACGCTCGAATTGCTGAACCATATTGCCGATACCAGCGTGCCGTTCACGCTGCTGGGCCGCG
GCCTGGTGTCGCTGGTGGTGGTGTTCACGATCGGCGCCGCGCTGTTGCGCGCCAACCGCGTGCGCAGCGGGCTGGCCGCG
GCCGGCGAGCGCGCCGAGGCACGCGCCACACTGGCGGGCATGATCCACGCCGGCGTCACGCTCGCCGTCATCGTTGCGCT
GATTGCGCTGCTCGCGGGCTATGTCAGCTTCGCCCGCTTCGTGACCTACGAACTGGTGTGGTTCGACATCGTGCTGTGCA
CGCTGTACCTGCTGACCCAGCTCACGCGCGACATCTTCGAAAGCCTGCTGTCGACGCAGCACGCAAGCGGCCGCGTGATC
CGGCAGCTGTTCGGCGTGGACGACAACCGCCTGGAGCAGGCATCGACGCTGCTGTCGGGCCTTGGCACCAGCGTGCTGAC
GATCGTTGCCATCATCGCGCTGCTGACCGGCGGCTTCGGCACCACGCCGGCGGAACTGCTCGACAGCCTGCTGACCGTGC
TCGGCGGCGACAAGCTGCGCAGCTATAACATCATGCCGGAGCGCATCATCAATGCCGTGATCGCGCTGTCGGTGGGCATC
TGGCTGCTGCGTTCGGTGCGGCGCTGGCTCGATGCGGAGCTGCTGCCGCGCATGTGCATGGAGCCGGGCCTGCGCGCGTC
GCTGGTCACGCTGTTCAGCAATGTCGGCTACGTGCTGATCGTACTGCTGACGCTGGCCCAGCTGGGGGTGAAGTGGGAGA
ACCTGGCATGGATCGTCAGCGCGCTGTCGGTGGGCATCGGCTTCGGCCTGCAGGAAATCGTCAAGAACTTCGTGTCGGGC
CTGATCCTGCTGACCGAGCGCCCGGTGAAGGTTGGCGACATGGTCAGCATCAGCGGCGTGGAAGGCGATATCCGCCGCAT
CAACGTGCGCGCAACCGAGATCCAGCTCGGCGACCGCTCCACGATGATCGTGCCGAACTCGCAGCTCATCTCGCAGAACG
TGCGCAACGTGACCATGAGCCAAAGCACGCAGGGCGTGGCCTCATTGCAGCTCACGTTCCCGCTCAACACCGATCCGGAG
CAGGTGCGCGACCTGCTGCTCGACACTTATCTGGATAACGAAACCATCCTCGACCACCCGGTGCCATCGGTCACCTTCAG
CCAGCTTGCACCCAACGGCATCACGCTGAGTGTGACGGGCTTCGTGGCCAACCCGCGCATTGCCGCGTCGACCAAGAGTG
ATCTGCTGTTCGAGATCCTCAAGCGCCTGCGTGCCGCCGGCATTTCGCTGACCATGCCGCAGACGCTGCGTGTCGAGAAC
CTGCCGGGCATGTCGGCGGAAGCGCTGCTGCCGGCGCCATGA

Upstream 100 bases:

>100_bases
TCCGCATCCTCAGGCATTCGCTCCCCGCCATCCGGCGCACAGTGGCTTGCCGGAGCGCGGCGGCAGTTTCCGGGCGGCCA
CGTCACCACGTGAATCCGCC

Downstream 100 bases:

>100_bases
CGCCGCGCACCGGCATGGGGCCGAGGCACCCGCACGTTGCCCCCCACGCTGCCAGATCGGTGCGCCGGCCGCGCGCCATG
CATCGGTCTGGCGCGCCGTG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MRRLSWLALSLVLTALFHLMPAALAAEPASAPEADQPPALTHAEAITELKRLQTEQDRIKQSASSVNGTTRLGDLDEGLH
QLAKDVDKLTAALTPQRAQLQAQLDVLGPPPAAGATPPEMPAVARQRAELNARKVQLDNALKQAAEGKENIENLNAQLAK
LQRSRMKDQLALRSESILNPQFWAPLFKPSDDDSARLEAFVDEVVPLLKAAWEPEQRGATALLLVLALAVWTLGRRLAER
AAAWFCLTRLPETRLRRSALALATALTTVVTTALAVQLVYYAFTRGYEPPTDLQDLLGALARLMLTSALIAGLGRALLCT
HHPSWRLPAMADPVARALKPFPRALAGLLLLAGTLELLNHIADTSVPFTLLGRGLVSLVVVFTIGAALLRANRVRSGLAA
AGERAEARATLAGMIHAGVTLAVIVALIALLAGYVSFARFVTYELVWFDIVLCTLYLLTQLTRDIFESLLSTQHASGRVI
RQLFGVDDNRLEQASTLLSGLGTSVLTIVAIIALLTGGFGTTPAELLDSLLTVLGGDKLRSYNIMPERIINAVIALSVGI
WLLRSVRRWLDAELLPRMCMEPGLRASLVTLFSNVGYVLIVLLTLAQLGVKWENLAWIVSALSVGIGFGLQEIVKNFVSG
LILLTERPVKVGDMVSISGVEGDIRRINVRATEIQLGDRSTMIVPNSQLISQNVRNVTMSQSTQGVASLQLTFPLNTDPE
QVRDLLLDTYLDNETILDHPVPSVTFSQLAPNGITLSVTGFVANPRIAASTKSDLLFEILKRLRAAGISLTMPQTLRVEN
LPGMSAEALLPAP

Sequences:

>Translated_813_residues
MRRLSWLALSLVLTALFHLMPAALAAEPASAPEADQPPALTHAEAITELKRLQTEQDRIKQSASSVNGTTRLGDLDEGLH
QLAKDVDKLTAALTPQRAQLQAQLDVLGPPPAAGATPPEMPAVARQRAELNARKVQLDNALKQAAEGKENIENLNAQLAK
LQRSRMKDQLALRSESILNPQFWAPLFKPSDDDSARLEAFVDEVVPLLKAAWEPEQRGATALLLVLALAVWTLGRRLAER
AAAWFCLTRLPETRLRRSALALATALTTVVTTALAVQLVYYAFTRGYEPPTDLQDLLGALARLMLTSALIAGLGRALLCT
HHPSWRLPAMADPVARALKPFPRALAGLLLLAGTLELLNHIADTSVPFTLLGRGLVSLVVVFTIGAALLRANRVRSGLAA
AGERAEARATLAGMIHAGVTLAVIVALIALLAGYVSFARFVTYELVWFDIVLCTLYLLTQLTRDIFESLLSTQHASGRVI
RQLFGVDDNRLEQASTLLSGLGTSVLTIVAIIALLTGGFGTTPAELLDSLLTVLGGDKLRSYNIMPERIINAVIALSVGI
WLLRSVRRWLDAELLPRMCMEPGLRASLVTLFSNVGYVLIVLLTLAQLGVKWENLAWIVSALSVGIGFGLQEIVKNFVSG
LILLTERPVKVGDMVSISGVEGDIRRINVRATEIQLGDRSTMIVPNSQLISQNVRNVTMSQSTQGVASLQLTFPLNTDPE
QVRDLLLDTYLDNETILDHPVPSVTFSQLAPNGITLSVTGFVANPRIAASTKSDLLFEILKRLRAAGISLTMPQTLRVEN
LPGMSAEALLPAP
>Mature_813_residues
MRRLSWLALSLVLTALFHLMPAALAAEPASAPEADQPPALTHAEAITELKRLQTEQDRIKQSASSVNGTTRLGDLDEGLH
QLAKDVDKLTAALTPQRAQLQAQLDVLGPPPAAGATPPEMPAVARQRAELNARKVQLDNALKQAAEGKENIENLNAQLAK
LQRSRMKDQLALRSESILNPQFWAPLFKPSDDDSARLEAFVDEVVPLLKAAWEPEQRGATALLLVLALAVWTLGRRLAER
AAAWFCLTRLPETRLRRSALALATALTTVVTTALAVQLVYYAFTRGYEPPTDLQDLLGALARLMLTSALIAGLGRALLCT
HHPSWRLPAMADPVARALKPFPRALAGLLLLAGTLELLNHIADTSVPFTLLGRGLVSLVVVFTIGAALLRANRVRSGLAA
AGERAEARATLAGMIHAGVTLAVIVALIALLAGYVSFARFVTYELVWFDIVLCTLYLLTQLTRDIFESLLSTQHASGRVI
RQLFGVDDNRLEQASTLLSGLGTSVLTIVAIIALLTGGFGTTPAELLDSLLTVLGGDKLRSYNIMPERIINAVIALSVGI
WLLRSVRRWLDAELLPRMCMEPGLRASLVTLFSNVGYVLIVLLTLAQLGVKWENLAWIVSALSVGIGFGLQEIVKNFVSG
LILLTERPVKVGDMVSISGVEGDIRRINVRATEIQLGDRSTMIVPNSQLISQNVRNVTMSQSTQGVASLQLTFPLNTDPE
QVRDLLLDTYLDNETILDHPVPSVTFSQLAPNGITLSVTGFVANPRIAASTKSDLLFEILKRLRAAGISLTMPQTLRVEN
LPGMSAEALLPAP

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=252, Percent_Identity=31.3492063492063, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI2367355, Length=302, Percent_Identity=28.8079470198675, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1789291, Length=195, Percent_Identity=25.1282051282051, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 87868; Mature: 87868

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLSWLALSLVLTALFHLMPAALAAEPASAPEADQPPALTHAEAITELKRLQTEQDRIK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QSASSVNGTTRLGDLDEGLHQLAKDVDKLTAALTPQRAQLQAQLDVLGPPPAAGATPPEM
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PAVARQRAELNARKVQLDNALKQAAEGKENIENLNAQLAKLQRSRMKDQLALRSESILNP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QFWAPLFKPSDDDSARLEAFVDEVVPLLKAAWEPEQRGATALLLVLALAVWTLGRRLAER
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
AAAWFCLTRLPETRLRRSALALATALTTVVTTALAVQLVYYAFTRGYEPPTDLQDLLGAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ARLMLTSALIAGLGRALLCTHHPSWRLPAMADPVARALKPFPRALAGLLLLAGTLELLNH
HHHHHHHHHHHHCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADTSVPFTLLGRGLVSLVVVFTIGAALLRANRVRSGLAAAGERAEARATLAGMIHAGVT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAVIVALIALLAGYVSFARFVTYELVWFDIVLCTLYLLTQLTRDIFESLLSTQHASGRVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQLFGVDDNRLEQASTLLSGLGTSVLTIVAIIALLTGGFGTTPAELLDSLLTVLGGDKLR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHC
SYNIMPERIINAVIALSVGIWLLRSVRRWLDAELLPRMCMEPGLRASLVTLFSNVGYVLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VLLTLAQLGVKWENLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSISGV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHEECCCCCCCCEEEECCC
EGDIRRINVRATEIQLGDRSTMIVPNSQLISQNVRNVTMSQSTQGVASLQLTFPLNTDPE
CCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
QVRDLLLDTYLDNETILDHPVPSVTFSQLAPNGITLSVTGFVANPRIAASTKSDLLFEIL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
KRLRAAGISLTMPQTLRVENLPGMSAEALLPAP
HHHHHCCCEEECCCHHEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRLSWLALSLVLTALFHLMPAALAAEPASAPEADQPPALTHAEAITELKRLQTEQDRIK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QSASSVNGTTRLGDLDEGLHQLAKDVDKLTAALTPQRAQLQAQLDVLGPPPAAGATPPEM
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PAVARQRAELNARKVQLDNALKQAAEGKENIENLNAQLAKLQRSRMKDQLALRSESILNP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QFWAPLFKPSDDDSARLEAFVDEVVPLLKAAWEPEQRGATALLLVLALAVWTLGRRLAER
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
AAAWFCLTRLPETRLRRSALALATALTTVVTTALAVQLVYYAFTRGYEPPTDLQDLLGAL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ARLMLTSALIAGLGRALLCTHHPSWRLPAMADPVARALKPFPRALAGLLLLAGTLELLNH
HHHHHHHHHHHHCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADTSVPFTLLGRGLVSLVVVFTIGAALLRANRVRSGLAAAGERAEARATLAGMIHAGVT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LAVIVALIALLAGYVSFARFVTYELVWFDIVLCTLYLLTQLTRDIFESLLSTQHASGRVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQLFGVDDNRLEQASTLLSGLGTSVLTIVAIIALLTGGFGTTPAELLDSLLTVLGGDKLR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHC
SYNIMPERIINAVIALSVGIWLLRSVRRWLDAELLPRMCMEPGLRASLVTLFSNVGYVLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VLLTLAQLGVKWENLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSISGV
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHEECCCCCCCCEEEECCC
EGDIRRINVRATEIQLGDRSTMIVPNSQLISQNVRNVTMSQSTQGVASLQLTFPLNTDPE
CCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
QVRDLLLDTYLDNETILDHPVPSVTFSQLAPNGITLSVTGFVANPRIAASTKSDLLFEIL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHH
KRLRAAGISLTMPQTLRVENLPGMSAEALLPAP
HHHHHCCCEEECCCHHEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]