Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 71911648

GI number: 71911648

Start: 1800257

End: 1801624

Strand: Reverse

Name: holB [H]

Synonym: M5005_Spy_1835

Alternate gene names: 71911648

Gene position: 1801624-1800257 (Counterclockwise)

Preceding gene: 71911649

Following gene: 71911647

Centisome position: 97.99

GC content: 38.96

Gene sequence:

>1368_bases
ATGAAGTTGCCTGAAGTAGCTGAATTACGAGTTCAACCCCAAGATTTACTAGCAGAGCAATCTGTTCTTGGGTCAATCTT
TATCTCACCTGATAAGCTGATTGCAGTGAGAGAATTTATCAGTCCAGACGATTTTTATAAGTACGCTCATAAAATTATCT
TTCGGGCAATGATTACCCTCAGCGATCGTAATGATGCCATTGATGCAACCACTATAAGAACAATCCTAGATGATCAAGAT
GATCTGCAAAGTATTGGTGGCTTATCCTATATTGTTGAACTAGTTAATAGTGTCCCAACTAGTGCTAATGCAGAATATTA
TGCTAAAATTGTAGCTGAGAAAGCTATGTTGCGTGATATTATTGCTAGGTTGACAGAATCTGTCAACCTAGCTTATGATG
AAATTTTAAAACCAGAAGAGGTTATCGCTGGAGTTGAGAGAGCTTTAATTGAACTCAATGAACATAGTAATCGTAGTGGG
TTTCGCAAAATTTCAGATGTGCTAAAAGTTAATTACGAGGCTTTAGAAGCACGTTCTAAGCAGACTTCAAATGTTACAGG
TTTACCAACTGGTTTTAGAGACCTTGACAAGATTACAACAGGTTTACACCCAGATCAATTAGTTATTTTAGCTGCTCGGC
CAGCAGTGGGGAAGACTGCCTTTGTTCTTAATATTGCGCAAAATGTGGGGACTAAGCAAAAAAAGACTGTTGCTATTTTT
TCTTTGGAAATGGGTGCTGAAAGTTTAGTAGATCGTATGCTTGCAGCAGAAGGAATGGTTGATTCGCACAGTTTAAGAAC
AGGGCAACTCACAGATCAGGATTGGAATAATGTAACAATTGCTCAGGGAGCTTTGGCAGAAGCACCGATTTATATTGACG
ATACGCCCGGGATTAAAATTACTGAAATCCGCGCAAGATCACGGAAATTGTCTCAAGAAGTGGATGGTGGTTTAGGTCTC
ATTGTAATTGACTACTTACAGTTGATTACAGGAACTAAACCCGAAAATCGTCAGCAAGAGGTTTCAGATATTTCAAGACA
GCTTAAAATCCTAGCTAAAGAATTGAAAGTACCAGTTATTGCCCTAAGTCAGCTTTCTCGTGGCGTTGAGCAAAGGCAAG
ATAAACGACCAGTTTTATCAGATATTCGTGAATCAGGATCTATTGAGCAGGATGCCGATATTGTAGCCTTCTTATACCGG
GACGATTATTACCGTAAAGAATGTGATGATGCTGAAGAAGCTGTTGAAGATAACACAATTGAAGTTATCCTCGAGAAAAA
TAGAGCTGGGGCGCGTGGAACAGTCAAACTGATGTTCCAAAAAGAATACAACAAATTCTCAAGTATAGCCCAGTTTGAAG
AAAGATAA

Upstream 100 bases:

>100_bases
TAATTGAAGTTCCTGTGAAATTACATAAGGAAGTAACAGCTGAGATTAAATTGGCTATCACAGAAGCATAATATAAAAAA
CATCACAAGATTTGGAGGTT

Downstream 100 bases:

>100_bases
GAAAGAGAGACAAAAAATGAGCGATGCATTTACAGATGTTGCAAAAATGAAAAAAATTAAAGAGGACATAAGAGCCCATG
AAGGACAGTTAGTAGAATTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQD
DLQSIGGLSYIVELVNSVPTSANAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTAFVLNIAQNVGTKQKKTVAIF
SLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGL
IVIDYLQLITGTKPENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVAFLYR
DDYYRKECDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFEER

Sequences:

>Translated_455_residues
MKLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQD
DLQSIGGLSYIVELVNSVPTSANAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTAFVLNIAQNVGTKQKKTVAIF
SLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGL
IVIDYLQLITGTKPENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVAFLYR
DDYYRKECDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFEER
>Mature_455_residues
MKLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQD
DLQSIGGLSYIVELVNSVPTSANAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTAFVLNIAQNVGTKQKKTVAIF
SLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGL
IVIDYLQLITGTKPENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVAFLYR
DDYYRKECDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFEER

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=453, Percent_Identity=42.8256070640177, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50757; Mature: 50757

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFYKYAHKIIFRAMITL
CCCCCCHHHCCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
SDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSANAEYYAKIVAEKAMLRDI
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSGFRKISDVLKVNYEALEARSK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
QTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTAFVLNIAQNVGTKQKKTVAIF
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHEEEEHHHCCCCCCCEEEEE
SLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVTIAQGALAEAPIYIDDTPGIKI
EECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCEEE
TEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKPENRQQEVSDISRQLKILAKELKVPVI
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVAFLYRDDYYRKECDDAEEAVEDNTI
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHCCCCE
EVILEKNRAGARGTVKLMFQKEYNKFSSIAQFEER
EEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFYKYAHKIIFRAMITL
CCCCCCHHHCCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
SDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSANAEYYAKIVAEKAMLRDI
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSGFRKISDVLKVNYEALEARSK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
QTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTAFVLNIAQNVGTKQKKTVAIF
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHEEEEHHHCCCCCCCEEEEE
SLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVTIAQGALAEAPIYIDDTPGIKI
EECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCEEE
TEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKPENRQQEVSDISRQLKILAKELKVPVI
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVAFLYRDDYYRKECDDAEEAVEDNTI
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHCCCCE
EVILEKNRAGARGTVKLMFQKEYNKFSSIAQFEER
EEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]