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The map label for this gene is tig [H]

Identifier: 71911425

GI number: 71911425

Start: 1569093

End: 1570376

Strand: Reverse

Name: tig [H]

Synonym: M5005_Spy_1612

Alternate gene names: 71911425

Gene position: 1570376-1569093 (Counterclockwise)

Preceding gene: 71911427

Following gene: 161936120

Centisome position: 85.41

GC content: 37.54

Gene sequence:

>1284_bases
ATGTCTACATCATTTGAAAACAAAGCTACAAATCGTGGCGTGATTACATTTACAATCAGTCAAGATAAAATAAAACCAGC
TCTTGATAAAGCTTTTAATAAAATCAAAAAAGACTTGAATGCACCAGGTTTCCGTAAAGGACACATGCCTCGTCCAGTCT
TCAATCAAAAATTTGGTGAAGAAGTTCTTTATGAAGATGCTTTGAATATTGTATTGCCAGAAGCTTATGAAGCAGCTGTG
ACGGAACTTGGTCTTGATGTGGTTGCACAACCAAAAATCGATGTTGTGTCAATGGAAAAAGGGAAAGAGTGGACACTTTC
TGCTGAAGTTGTGACAAAACCTGAAGTGAAACTTGGTGATTACAAAAACCTAGTTGTAGAAGTTGATGCTTCAAAAGAAG
TTTCAGATGAAGACGTGGATGCTAAAATTGAGCGCGAACGTCAAAACCTTGCGGAACTCATTATTAAAGACGGTGAAGCA
GCTCAAGGTGACACTGTTGTGATTGACTTTGTTGGTTCAGTTGATGGTGTTGAGTTTGATGGCGGTAAAGGAGATAACTT
CTCTCTTGAACTTGGTTCAGGACAATTTATCCCAGGTTTTGAAGATCAACTAGTTGGTGCTAAAGCTGGCGATGAAGTAG
AAGTTAATGTCACATTCCCAGAATCTTACCAAGCAGAAGATCTTGCAGGTAAAGCCGCTAAATTTATGACAACTATTCAC
GAAGTCAAAACAAAAGAAGTACCAGAGCTTGATGATGAGCTTGCAAAAGATATTGATGAAGATGTTGACACACTTGAAGA
CTTAAAAGTAAAATATCGTAAAGAACTTGAAGCAGCTCAAGAAACTGCTTATGATGATGCTGTTGAAGGAGCTGCGATTG
AATTAGCAGTTGCAAATGCTGAAATTGTTGATTTACCCGAAGAAATGATTCATGAAGAAGTCAACCGTTCAGTGAATGAA
TTTATGGGCAACATGCAACGTCAAGGAATCTCACCTGAAATGTACTTCCAATTGACTGGTACAACTCAAGAAGATTTACA
TAACCAATATTCAGCTGAAGCTGACAAACGTGTTAAAACAAACCTTGTTATTGAAGCAATTGCTAAAGCAGAAGGTTTTG
AAGCAACAGATAGTGAAATTGAACAAGAAATTAATGACCTTGCAACAGAATATAACATGCCAGCTGACCAAGTTCGTTCT
CTTCTTTCAGCAGATATGTTGAAACATGATATTGCAATGAAAAAAGCAGTTGAAGTGATTACAAGCACAGCAAGCGTTAA
GTAA

Upstream 100 bases:

>100_bases
CTTAATAGCGCGTTTGATTTCACGCATAAAAAGCTTTAAAAACGCTTTAAATTATGATAAAATGAACTGTATCGAAATTA
TTTTAAGGAGAAATGACTTA

Downstream 100 bases:

>100_bases
TCTTATTAAGGAACAAAACGTACAAGCCGGATTAGAACGTCTTTCTAGTCTGGTTTTTCTTTTTCTAAAGAAATAATAAG
GAATTAGTTAAACGTTATTT

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGFRKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAV
TELGLDVVAQPKIDVVSMEKGKEWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVTFPESYQAEDLAGKAAKFMTTIH
EVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKELEAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNE
FMGNMQRQGISPEMYFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYNMPADQVRS
LLSADMLKHDIAMKKAVEVITSTASVK

Sequences:

>Translated_427_residues
MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGFRKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAV
TELGLDVVAQPKIDVVSMEKGKEWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVTFPESYQAEDLAGKAAKFMTTIH
EVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKELEAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNE
FMGNMQRQGISPEMYFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYNMPADQVRS
LLSADMLKHDIAMKKAVEVITSTASVK
>Mature_426_residues
STSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGFRKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVT
ELGLDVVAQPKIDVVSMEKGKEWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEAA
QGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVTFPESYQAEDLAGKAAKFMTTIHE
VKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKELEAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEF
MGNMQRQGISPEMYFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYNMPADQVRSL
LSADMLKHDIAMKKAVEVITSTASVK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=421, Percent_Identity=30.6413301662708, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: 5.2.1.8

Molecular weight: Translated: 47096; Mature: 46965

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGFRKGHMPRPVFNQKFGE
CCCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGKEWTLSAEVVTKPEVKLGD
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCC
YKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEAAQGDTVVIDFVGSVDGVEFD
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEC
GGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVTFPESYQAEDLAGKAAKFMTTIH
CCCCCCEEEEECCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKELEAAQETAYDDAVEGAAIELAVANA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
EIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEMYFQLTGTTQEDLHNQYSAEADKRVKT
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
NLVIEAIAKAEGFEATDSEIEQEINDLATEYNMPADQVRSLLSADMLKHDIAMKKAVEVI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTASVK
HHHCCCC
>Mature Secondary Structure 
STSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGFRKGHMPRPVFNQKFGE
CCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGKEWTLSAEVVTKPEVKLGD
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCC
YKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEAAQGDTVVIDFVGSVDGVEFD
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEC
GGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVTFPESYQAEDLAGKAAKFMTTIH
CCCCCCEEEEECCCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKELEAAQETAYDDAVEGAAIELAVANA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
EIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEMYFQLTGTTQEDLHNQYSAEADKRVKT
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
NLVIEAIAKAEGFEATDSEIEQEINDLATEYNMPADQVRSLLSADMLKHDIAMKKAVEVI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSTASVK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA