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The map label for this gene is pyrG [H]

Identifier: 71911422

GI number: 71911422

Start: 1566441

End: 1567895

Strand: Reverse

Name: pyrG [H]

Synonym: M5005_Spy_1609

Alternate gene names: 71911422

Gene position: 1567895-1566441 (Counterclockwise)

Preceding gene: 71911423

Following gene: 71911421

Centisome position: 85.28

GC content: 41.17

Gene sequence:

>1455_bases
ATGAGCCCCTATCAGCATGGAGAAGTATATGTGACAGATGACGGTGCTGAAACCGACTTGGATTTGGGTCACTATGAACG
TTTTATTGATATCAATCTTAATAAATATTCCAATGTTACAACAGGTAAAATTTATAGTGAAGTCCTTCGTAAAGAACGTA
AAGGCGAGTACCTTGGAGCGACTGTTCAGGTGATTCCTCATATCACAGATGCCCTTAAAGAAAAAATCAAACGCGCAGCT
TCAACAACGGACTCTGATGTAATTATTACTGAGGTCGGGGGGACTGTTGGCGATATTGAAAGTTTACCGTTCTTAGAAGC
TCTTCGTCAGATGAAAGCTGATGTTGGTTCTGAAAATGTGATGTATATTCACACCACCTTGTTGCCATATTTAAAGGCAG
CGGGTGAAATGAAAACTAAGCCAACGCAACATTCCGTTAAAGAATTGCGTGGTTTAGGGATTCAACCGAATATGTTGGTT
ATTCGTACGGAAGAGCCAGTTGAGCAGGGGATTAAAAATAAATTGGCTCAGTTCTGTGATGTGAATTCAGAAGCTGTTAT
CGAGTCACGTGATGTAGAACACTTGTATCAAATTCCATTGAACTTGCAAGCTCAATCAATGGATCAGATTGTTTGTGATC
ATTTGAAATTGAATGCGCCACAAGCTGATATGACCGAGTGGTCAGCTATGGTTGACAAGGTGATGAATTTAAGAAAAACA
ACAAAAATTGCCTTGGTTGGTAAATATGTGGAATTGCCAGATGCCTACTTATCTGTTGTGGAAGCCTTGAAACATTCTGG
TTATGCCAATGATACCGCAATTGACCTAAAATGGGTCAATGCTAACGATGTGACGGTGGATAATGCAGCTGACTTGTTGG
GAGATGCGGATGGGATTATTGTACCAGGTGGTTTTGGTCAACGTGGTACAGAAGGCAAGATCCAAGCGATTCGCTATGCC
CGCGAAAATGATGTGCCAATGCTTGGTATTTGTCTTGGCATGCAGTTGACTTGTGTTGAATTTGCCCGCCATGTCCTCAA
TATGGAAGGTGCAAACTCTTTTGAATTAGAGCCAAGTACTAAATACCCCATCATTGATATCATGCGTGATCAAATTGATA
TTGAAGATATGGGAGGTACGCTTCGTCTGGGATTGTACCCATGTAAATTGAAACCAGGTTCAAAAGCAGCAATGGCTTAC
AATAATCAAGAGGTGGTGCAACGTCGTCATCGTCACCGTTATGAGTTCAACAACAAATTCCGTCCAGAATTTGAAGCAGC
TGGTTTTGTTTTCTCAGGGGTATCACCTGACAACCGCTTAGTTGAAATTGTAGAATTGAAAGAGAAAAAATTCTTTGTAG
CAGCTCAATACCACCCAGAATTGCAAAGCCGTCCAAATCGTCCTGAGGAGCTATACACCGCTTTTGTTACAGCTGCTATT
AAAAATAGCAATTAG

Upstream 100 bases:

>100_bases
AAGGAATTGTTGCGGCAAGTCTTGGCCGATTGTTAAAAAATCGTGGACTTAAGGTGACGATTCAAAAATTTGATCCTTAT
ATTAATATTGATCCAGGACG

Downstream 100 bases:

>100_bases
TGGTTTTAAGGGTAAGTTTGTGGCATTCTACTTGGCTGGCAGATACTGATAAAGATTACTAGCTATTAAAGGGATCTTTT
CTATAAAAATAGCATTAGAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERKGEYLGATVQVIPHITDALKEKIKRAA
STTDSDVIITEVGGTVGDIESLPFLEALRQMKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLV
IRTEEPVEQGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTEWSAMVDKVMNLRKT
TKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDVTVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYA
RENDVPMLGICLGMQLTCVEFARHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYHPELQSRPNRPEELYTAFVTAAI
KNSN

Sequences:

>Translated_484_residues
MSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERKGEYLGATVQVIPHITDALKEKIKRAA
STTDSDVIITEVGGTVGDIESLPFLEALRQMKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLV
IRTEEPVEQGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTEWSAMVDKVMNLRKT
TKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDVTVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYA
RENDVPMLGICLGMQLTCVEFARHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYHPELQSRPNRPEELYTAFVTAAI
KNSN
>Mature_483_residues
SPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERKGEYLGATVQVIPHITDALKEKIKRAAS
TTDSDVIITEVGGTVGDIESLPFLEALRQMKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVI
RTEEPVEQGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTEWSAMVDKVMNLRKTT
KIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDVTVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYAR
ENDVPMLGICLGMQLTCVEFARHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAYN
NQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYHPELQSRPNRPEELYTAFVTAAIK
NSN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=503, Percent_Identity=45.3280318091451, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI28559085, Length=506, Percent_Identity=43.8735177865613, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI28559083, Length=506, Percent_Identity=43.8735177865613, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI221316689, Length=506, Percent_Identity=43.8735177865613, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789142, Length=489, Percent_Identity=49.8977505112474, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI25148299, Length=557, Percent_Identity=34.8294434470377, Blast_Score=353, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6319432, Length=514, Percent_Identity=42.9961089494163, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322563, Length=511, Percent_Identity=42.4657534246575, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24664469, Length=504, Percent_Identity=42.8571428571429, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI21357815, Length=497, Percent_Identity=42.4547283702213, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 54187; Mature: 54056

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERKGEYLGA
CCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
TVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQMKADVGSENV
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCE
MYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVEQGIKNKLAQFCD
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
VNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTEWSAMVDKVMNLRKT
CCCHHEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
TKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDVTVDNAADLLGDADGII
HEEEEEECHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCHHHHHCCCCCEE
VPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFARHVLNMEGANSFELEPST
ECCCCCCCCCCCHHHHEEECCCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCCCEECCCCC
KYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAYNNQEVVQRRHRHRYEFNNKF
CCCHHHHHHHHCCHHHCCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCC
RPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYHPELQSRPNRPEELYTAFVTAAI
CCCHHCCCEEEECCCCCCHHHHHEECCCCEEEEEEECCCHHHCCCCCHHHHHHHHHHHHC
KNSN
CCCC
>Mature Secondary Structure 
SPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERKGEYLGA
CCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
TVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQMKADVGSENV
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCE
MYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVEQGIKNKLAQFCD
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
VNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTEWSAMVDKVMNLRKT
CCCHHEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
TKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDVTVDNAADLLGDADGII
HEEEEEECHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCHHHHHCCCCCEE
VPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFARHVLNMEGANSFELEPST
ECCCCCCCCCCCHHHHEEECCCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCCCEECCCCC
KYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAYNNQEVVQRRHRHRYEFNNKF
CCCHHHHHHHHCCHHHCCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCC
RPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYHPELQSRPNRPEELYTAFVTAAI
CCCHHCCCEEEECCCCCCHHHHHEECCCCEEEEEEECCCHHHCCCCCHHHHHHHHHHHHC
KNSN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA