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The map label for this gene is yckB [H]

Identifier: 71910519

GI number: 71910519

Start: 708810

End: 709673

Strand: Direct

Name: yckB [H]

Synonym: M5005_Spy_0706

Alternate gene names: 71910519

Gene position: 708810-709673 (Clockwise)

Preceding gene: 71910518

Following gene: 71910520

Centisome position: 38.55

GC content: 38.19

Gene sequence:

>864_bases
ATGAAGAAATCAGTTACATTACTGTCTATCGGACTTGCCAGCCTTTTATTGGCAGCATGTGCGCCACATCAATCTCAAAA
ATCAAGTTGGGACACTATCAAAGAAAAAGGGGTGTTAAAAGTAGCTACCCCAGGAACCTATCAGCCAACTTCTTTTTACA
ACGATAATAATGAGTTAGTAGGTTACGAAGTAGATATGGTCAAAGAAATCGGTAAACGACTTAACATTAAAGTCAAGTTT
GTTGAAACAGGATTTGACCAAGCCTTTACCTCAGTTGATAGTGGTCGAGTGGATATTTCTTTGAACAACTTTGACATTAC
CCCAAAACGTCAGAAAAAATACAATATCTCTACGCCTTATAAATACGGGGTAGGAGGCATGATTGTCCGTGCTGATGGCA
GCTCAAACATGGCTAAAAAAGATCTTAGCGACTGGAAAGGAAAAAAAGCAGCCGGCGCTTCTGGAACAGAATACATGAAA
GTAGCGCAAAAACAGGGAGCTGAACTGGTGACCTACGATAATGTTACAGGAGATGTTTATCTAAACGATGTGGCTAACGG
TCGAACAGATTTCATCCCAAATGATTACCCAGCTCAAAAATTATTTGTGGATTACATGTTATCTCAAAATCCAAATCTAA
ATGTCAAAATGAGCGATGTTCAGTACAACCCAACCGAACAAGGAATTGTTATGAACAAAAAAGACGATAGCCTCAAGAAA
AAAATAGATGCTGTTATTAAAGACATGATAAAAGATGGTAGCTTGAAGAAAATCTCTGAAACCTACCATGCTGGTCAAGA
TCTGACAAAACCTTTTGGCAAAGACAAAAAAATTCCCGTCATTGATACGAAAGACGTTAACTAA

Upstream 100 bases:

>100_bases
TTAACTATTATAGATTAACTTTTTAAAGAGATTGAGTGCTCACTGATAACACAACTTAATCAAAATGTTATACTATTCTT
ATCTTAATAAAGGAGTTACT

Downstream 100 bases:

>100_bases
TACTTGATGTTAAAGGAGTCCTAACCACATGATTAATATCCCATTAATGAAAGATAGTTTAGGATTTGTGTTATCGGGAT
TACCTTATACCCTTGGTATT

Product: cystine-binding protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATPGTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKF
VETGFDQAFTSVDSGRVDISLNNFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMSDVQYNPTEQGIVMNKKDDSLKK
KIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDKKIPVIDTKDVN

Sequences:

>Translated_287_residues
MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATPGTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKF
VETGFDQAFTSVDSGRVDISLNNFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMSDVQYNPTEQGIVMNKKDDSLKK
KIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDKKIPVIDTKDVN
>Mature_287_residues
MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATPGTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKF
VETGFDQAFTSVDSGRVDISLNNFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMSDVQYNPTEQGIVMNKKDDSLKK
KIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDKKIPVIDTKDVN

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=267, Percent_Identity=31.4606741573034, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787085, Length=268, Percent_Identity=29.4776119402985, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787088, Length=226, Percent_Identity=28.7610619469027, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=24.7826086956522, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788649, Length=269, Percent_Identity=25.2788104089219, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31792; Mature: 31792

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATPGTYQPTSFYNDNNELV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCEECCCCCEE
GYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLNNFDITPKRQKKYNISTPY
EEEHHHHHHHHHHCEEEEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCE
KYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMKVAQKQGAELVTYDNVTGDVY
EECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEE
LNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMSDVQYNPTEQGIVMNKKDDSLKK
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEECCCCCCEEEECCCHHHHH
KIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDKKIPVIDTKDVN
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCEECCCCCC
>Mature Secondary Structure
MKKSVTLLSIGLASLLLAACAPHQSQKSSWDTIKEKGVLKVATPGTYQPTSFYNDNNELV
CCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCEECCCCCEE
GYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLNNFDITPKRQKKYNISTPY
EEEHHHHHHHHHHCEEEEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCE
KYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMKVAQKQGAELVTYDNVTGDVY
EECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEE
LNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMSDVQYNPTEQGIVMNKKDDSLKK
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCEECCCCCCEEEECCCHHHHH
KIDAVIKDMIKDGSLKKISETYHAGQDLTKPFGKDKKIPVIDTKDVN
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 9384377 [H]