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The map label for this gene is papS

Identifier: 71910486

GI number: 71910486

Start: 675044

End: 676252

Strand: Direct

Name: papS

Synonym: M5005_Spy_0673

Alternate gene names: 71910486

Gene position: 675044-676252 (Clockwise)

Preceding gene: 71910485

Following gene: 71910487

Centisome position: 36.72

GC content: 39.37

Gene sequence:

>1209_bases
ATGAAATTAATGACAATGCCTTCTGAGTTTCAGAAGGCTTTACCCATATTGACTAAAATAAAAGAAGCTGGTTATGAAGC
CTATTTTGTGGGAGGAAGCGTCAGAGATGTTTTACTCGAGCGTCCCATTCATGATGTAGATATTGCAACTAGTTCCTATC
CTGAAGAGACTAAAGCGATTTTTAACAGAACTGTCGATGTTGGCATTGAACATGGTACAGTGCTCGTGCTTGAAAATGGC
GGTGAGTATGAAATTACAACCTTTAGGACAGAAGATATCTATGTTGATTATCGCCGTCCTAGTCAGGTTTCTTTTGTGCG
CTCTTTAGAGGAAGACTTAAAACGAAGAGACTTTACCGTCAATGCCTTGGCCTTGGATGAAAATGGTCAAGTTATTGATA
AATTTAGAGGACTCATTGATTTAAAGCAAAAACGGTTACGAGCAGTGGGTAAAGCAGAGGAACGTTTTGAAGAAGATGCA
CTACGCATTATGAGAGGCTTTCGATTTGCGGCGAGTCTGGATTTTGACATTGAAGCTATAACTTTTGAAGCTATGAGGAG
CCATTCACCTCTATTGGAAAAGATTTCTGTGGAACGTTCTTTTACTGAATTTGATAAATTATTGATGGCACCGCACTGGC
GAAAAGGGATTTCTGCCATGATTGCTTGTCAGGCTTACGACTATTTACCAGGCTTGAAGCAGCAGGAAGCAGGCTTGAAT
CATCTGATAGTTTCTTTGAAAGATAACTTTACCTTTAGTGATTATCACCAAGCTTGGGCTTATGTGATGATTAGTTTAGC
CATTGAGGATCCTAAAAGCTTCCTAAAAGCATGGAAGACTTCAAATGATTTTCAGCGTTATGTCACAAAGTTGATTGCTC
TTTATAGAATTCGTCAGGAAAGATCTTTTGAAAAATTGGATATTTACCAGTACGGCAAGAAAATGGCGAGTCTAGTGGAA
GACCTCCGAAAAGCTCAAAGCTTATCCGTTGACATGGATCGTATTAATACTTTAGATCAGGCACTGGTTATTCATGACAA
ACACGATATCGTCCTTAATGGTTCTCACCTGATTAAGGATTTTGGGATGAAATCAGGTCCTCAGCTAGGGCTTATGCTTG
AGAAAGTTGAATTAGCGATTGTAGAGGGGCGCCTAGATAATGATTTCACTACAATTGAAGCATTTGTTAGAGAGGAATTA
GCAACATGA

Upstream 100 bases:

>100_bases
TGGCAAGAATCACTAAAACAATAACAAAATGTGATAGAACCCAATTAGCCTAATCACACTTTAGATTGGGCTAGTTGGAT
GATAGGTTAGAAAACAACGT

Downstream 100 bases:

>100_bases
GCCATTTTTTGGTGGAGAAATTAACAAAAACGGTGGGAGATAAGACCGTCTTTCAAGATATTTCCTTTATTATTCATGAC
TTTGACCGTATTGGGATTAT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVE
DLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT

Sequences:

>Translated_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVE
DLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT
>Mature_402_residues
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENG
GEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
LRIMRGFRFAASLDFDIEAITFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVE
DLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKDFGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREEL
AT

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=226, Percent_Identity=36.7256637168142, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI87081691, Length=211, Percent_Identity=35.5450236966825, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789436, Length=195, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=234, Percent_Identity=35.042735042735, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360112, Length=386, Percent_Identity=30.8290155440415, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21357337, Length=386, Percent_Identity=30.8290155440415, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_STRP1 (Q9A0A5)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269068.1
- RefSeq:   YP_282036.1
- ProteinModelPortal:   Q9A0A5
- SMR:   Q9A0A5
- EnsemblBacteria:   EBSTRT00000001513
- EnsemblBacteria:   EBSTRT00000028733
- GeneID:   3572224
- GeneID:   901027
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0866
- KEGG:   spz:M5005_Spy_0673
- GeneTree:   EBGT00050000028148
- HOGENOM:   HBG635063
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- BioCyc:   SPYO160490:SPY0866-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0673-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46235; Mature: 46235

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 35-35 BINDING 116-116 BINDING 159-159 BINDING 162-162 BINDING 165-165 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAI
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
HLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCEEECCHHHHHH
FGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIHDVDIATSSYPEETKAI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FNRTVDVGIEHGTVLVLENGGEYEITTFRTEDIYVDYRRPSQVSFVRSLEEDLKRRDFTV
HHHHHEEEECCCEEEEEECCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHHHHHCCCEE
NALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDALRIMRGFRFAASLDFDIEAI
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGISAMIACQAYDYLPGLKQQEAGLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCC
HLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLKAWKTSNDFQRYVTKLIALYRIRQE
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDRINTLDQALVIHDKHDIVLNGSHLIKD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCEEECCHHHHHH
FGMKSGPQLGLMLEKVELAIVEGRLDNDFTTIEAFVREELAT
CCCCCCCCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296