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The map label for this gene is pheT [H]

Identifier: 71910401

GI number: 71910401

Start: 586212

End: 588632

Strand: Direct

Name: pheT [H]

Synonym: M5005_Spy_0588

Alternate gene names: 71910401

Gene position: 586212-588632 (Clockwise)

Preceding gene: 71910400

Following gene: 71910402

Centisome position: 31.88

GC content: 42.3

Gene sequence:

>2421_bases
TTGGGCACTAAAATTATGTTAGTATCTTATAAATGGTTAAAAGAATTAGTAGATATCGATGTGACACCAGCAGCACTTGC
TGAGAAAATGTCGACAACGGGTATCGAGGTAGAAGGAATTGAAGTCCCTGCAGATGGTTTATCAAAACTGGTTGTGGGGC
ATGTGCTTTCTTGTGAAGATGTGCCAGAGACACATTTGCATTTGTGTCAGGTAGATACAGGTGATGAAACGCCACGTCAA
ATTGTGTGTGGCGCACCAAATGTGAAAGCAGGGATTAAGGTTATCGTGGCTGTGCCAGGTGCTCGTATTGCGGATAACTA
TAAAATCAAAAAAGGCAAAATCCGCGGCATGGAATCTCTTGGCATGATTTGTTCCTTGCAAGAACTTGGTTTGTCAGATA
GTATTATTCCAAAAGAATTTTCAGATGGTATTCAAATCTTGCCAGAGGAGGCTGTGCCAGGTGATGCTATTTTTAAATAC
CTGGACTTGGACGATCATATCATTGAATTGTCCATTACACCTAACCGTGCTGATGCGCTTTCTATGCGAGGTGTGGCACA
TGAAGTCGCAGCTATTTATGGCAAGTCAGTATCATTCCCCCAAAAAAATCTACAAGAATCTGATAAGGCAACTTCAGAAG
CCATTGAAGTGGCCATTGCCTCTGATAACGTTCTAACTTACGCTAGCCGTGTCGTTGAAAATGTCAAAGTTAAGCCAAGT
CCACAATGGTTGCAAAACCTTTTAATGAATGCTGGTATTCGTCCTATTAATAATGTGGTTGATGTGACCAACTATGTGCT
CTTGTATTTTGGTCAACCTATGCATGCTTTTGATTATGATAAATTTGAAGACCATAAGATTGTGGCGCGTGCGGCTCGTC
AAGGAGAAAGTTTGGTCACACTTGATGGTGAAAAACGGGATTTGACCACTGAGGATCTTGTTATTACCGTGGCTGACAAA
CCAGTTGCTTTAGCAGGTGTTATGGGTGGTCAAGCCACTGAAATTGATGCCAACTCTCAGACGGTTGTTCTTGAAGCTGC
TGTTTTTGATGGCAAATCGATTCGTAAAACCAGTGGTCGTTTAAACCTTCGTTCGGAATCCTCTTCACGTTTTGAAAAAG
GGGTTAACTATGCAACTGTTTTAGAAGCACTTGACTTTGCAGCGGCTATGTTACAAGAATTAGCAGAAGGTCAAGTCTTA
TCAGGCCATGTTCAAGCTGGACAATTGCCAACAGAACCTGTTGAAGTCTCAACTAGCCTTGACTATGTGAATGTTCGTTT
AGGAACAGAATTAACTTTTGCTGATATTCAAAGGATTTTTGACCAACTTGGATTTGGTCTAACAGGAGATGAAACAAGCT
TCACGGTAGCTGTTCCTCGGCGTCGTTGGGATGTTAGCATCCCAGCTGACTTGGTGGAAGAAATTGCGCGTATCTATGGT
TATGATAAGTTGCCAACAACCTTACCAGAGGCAGGGGGAACGGCTGCAGAATTAACGCCAACACAGGCTCTTCGTCGTAA
AGTCCGTGGCTTAGCGGAAGGATTGGGCTTGACTGAAATCATTTCGTATGCTTTGACAACACCAGAGAAAGCTGTTGAGT
TTGCAGTGGCACCAAGTCACCTGACAGAACTCATGTGGCCGATGTCTGTGGAGCGTTCAGCACTACGTCAAAATATGGTT
TCAGGGATGTTAGATACGGTTGCCTATAATGTGGCTCGTAAACAAAGTAACTTGGCTCTTTATGAAATTGGAAAAATCTT
TGAACAAGAAGCTAATCCAAAAGAAGACCTTCCTAATGAAGTGAACCATTTTGCTTTTGCTATTTGTGGCTTAGTGGCAC
AAAAAGATTTCCAAACCCAAGCTCAGGCAGTGGATTTTTACCATGCTAAAGGTAACTTGGATACTTTATTTGCAAACCTT
AACCTCAAGGTTCAATATGTGCCAACCAAAGACCTAGCAAACATGCACCCAGGACGGACAGCGCTTATCCTATTAGATGA
GCAAGTGATTGGATTTGTTGGTCAAGTTCATCCAGGAACAGCAAAGGCTTATAGTATTCCAGAAACCTATGTGGCAGAAT
TGGATATGGCTGCTTTAGAAGCAGCGTTGCCAAGTGATCAAACTTTTGCGGAAATCACAAAATTCCCTGCTATGACACGT
GATGTTGCCTTGTTACTTGACCGTGAGGTGAGTCATCAGGCCATTGTAACAGCTATTGAATCAGCAGGTGTAAAACGCTT
GACTAGCATTAAACTCTTTGACGTCTATGAAGGAGCAACCATTCAGGCTGGCAAAAAATCAATGGCTTATAGCCTTACTT
TCCAAAATCCAAATGACAATTTAACGGATGAAGAAGTTGCCAAATACATGGAGAAAATCACCAAAGCATTGACAGAACAA
GTCGGTGCTGAAGTACGCTAA

Upstream 100 bases:

>100_bases
TCCCTCTCGCTTCAACGTCATAAGGGTTTTGACTTGGAACTTAAAATACGAAAGGAAATGACTCAAATCACGGCTTGAGA
GCTTTCTCTAGCCGAGTGAT

Downstream 100 bases:

>100_bases
GAAAAAAACTGGGCCTCTAAGGCTCCGTTTTTTTCTGTTATAATAGAGGAAAAAAGAACTCCAGTTCAAATGATTGGAGA
AAAACATCAGAAGGTGGAGG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MGTKIMLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSKLVVGHVLSCEDVPETHLHLCQVDTGDETPRQ
IVCGAPNVKAGIKVIVAVPGARIADNYKIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKY
LDLDDHIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDNVLTYASRVVENVKVKPS
PQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDKFEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADK
PVALAGVMGGQATEIDANSQTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEGQVL
SGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFTVAVPRRRWDVSIPADLVEEIARIYG
YDKLPTTLPEAGGTAAELTPTQALRRKVRGLAEGLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMV
SGMLDTVAYNVARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHAKGNLDTLFANL
NLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIPETYVAELDMAALEAALPSDQTFAEITKFPAMTR
DVALLLDREVSHQAIVTAIESAGVKRLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQ
VGAEVR

Sequences:

>Translated_806_residues
MGTKIMLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSKLVVGHVLSCEDVPETHLHLCQVDTGDETPRQ
IVCGAPNVKAGIKVIVAVPGARIADNYKIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKY
LDLDDHIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDNVLTYASRVVENVKVKPS
PQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDKFEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADK
PVALAGVMGGQATEIDANSQTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEGQVL
SGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFTVAVPRRRWDVSIPADLVEEIARIYG
YDKLPTTLPEAGGTAAELTPTQALRRKVRGLAEGLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMV
SGMLDTVAYNVARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHAKGNLDTLFANL
NLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIPETYVAELDMAALEAALPSDQTFAEITKFPAMTR
DVALLLDREVSHQAIVTAIESAGVKRLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQ
VGAEVR
>Mature_805_residues
GTKIMLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSKLVVGHVLSCEDVPETHLHLCQVDTGDETPRQI
VCGAPNVKAGIKVIVAVPGARIADNYKIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYL
DLDDHIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDNVLTYASRVVENVKVKPSP
QWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDKFEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKP
VALAGVMGGQATEIDANSQTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEGQVLS
GHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFTVAVPRRRWDVSIPADLVEEIARIYGY
DKLPTTLPEAGGTAAELTPTQALRRKVRGLAEGLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVS
GMLDTVAYNVARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHAKGNLDTLFANLN
LKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIPETYVAELDMAALEAALPSDQTFAEITKFPAMTRD
VALLLDREVSHQAIVTAIESAGVKRLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQV
GAEVR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=822, Percent_Identity=34.4282238442822, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87798; Mature: 87667

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTKIMLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSKLVVGHVLSCED
CCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCC
VPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNYKIKKGKIRGMESL
CCCCCEEEEEECCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCEEECCCCCCHHHH
GMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDDHIIELSITPNRADAL
HHHHHHHHCCCCCCCCCHHHCCCCEECCCCCCCHHHHHEEECCCCEEEEEEECCCCHHHH
SMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDNVLTYASRVVENVKVKPS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCC
PQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDKFEDHKIVARAARQGESLVT
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEE
LDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANSQTVVLEAAVFDGKSIRKTSGR
ECCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCCCCCC
LNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEGQVLSGHVQAGQLPTEPVEVSTSL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCCCCEEEECCC
DYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFTVAVPRRRWDVSIPADLVEEIARIYG
CEEEEEECCEECHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC
YDKLPTTLPEAGGTAAELTPTQALRRKVRGLAEGLGLTEIISYALTTPEKAVEFAVAPSH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEECHHH
LTELMWPMSVERSALRQNMVSGMLDTVAYNVARKQSNLALYEIGKIFEQEANPKEDLPNE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCHH
VNHFAFAICGLVAQKDFQTQAQAVDFYHAKGNLDTLFANLNLKVQYVPTKDLANMHPGRT
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECEEEEEEECCHHHHCCCCCCE
ALILLDEQVIGFVGQVHPGTAKAYSIPETYVAELDMAALEAALPSDQTFAEITKFPAMTR
EEEEECCHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
DVALLLDREVSHQAIVTAIESAGVKRLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDN
HHHHHHCCCCCHHHHHHHHHHCCCHHHHEEEEEEEECCCEEECCCCCEEEEEEEECCCCC
LTDEEVAKYMEKITKALTEQVGAEVR
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTKIMLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSKLVVGHVLSCED
CCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHCCCC
VPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNYKIKKGKIRGMESL
CCCCCEEEEEECCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCEEECCCCCCHHHH
GMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDDHIIELSITPNRADAL
HHHHHHHHCCCCCCCCCHHHCCCCEECCCCCCCHHHHHEEECCCCEEEEEEECCCCHHHH
SMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDNVLTYASRVVENVKVKPS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCC
PQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDKFEDHKIVARAARQGESLVT
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEE
LDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANSQTVVLEAAVFDGKSIRKTSGR
ECCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCCCCCC
LNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEGQVLSGHVQAGQLPTEPVEVSTSL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCCCCEEEECCC
DYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFTVAVPRRRWDVSIPADLVEEIARIYG
CEEEEEECCEECHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC
YDKLPTTLPEAGGTAAELTPTQALRRKVRGLAEGLGLTEIISYALTTPEKAVEFAVAPSH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEECHHH
LTELMWPMSVERSALRQNMVSGMLDTVAYNVARKQSNLALYEIGKIFEQEANPKEDLPNE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCHH
VNHFAFAICGLVAQKDFQTQAQAVDFYHAKGNLDTLFANLNLKVQYVPTKDLANMHPGRT
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECEEEEEEECCHHHHCCCCCCE
ALILLDEQVIGFVGQVHPGTAKAYSIPETYVAELDMAALEAALPSDQTFAEITKFPAMTR
EEEEECCHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHH
DVALLLDREVSHQAIVTAIESAGVKRLTSIKLFDVYEGATIQAGKKSMAYSLTFQNPNDN
HHHHHHCCCCCHHHHHHHHHHCCCHHHHEEEEEEEECCCEEECCCCCEEEEEEEECCCCC
LTDEEVAKYMEKITKALTEQVGAEVR
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA