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The map label for this gene is metG

Identifier: 71910158

GI number: 71910158

Start: 351377

End: 353374

Strand: Direct

Name: metG

Synonym: M5005_Spy_0345

Alternate gene names: 71910158

Gene position: 351377-353374 (Clockwise)

Preceding gene: 71910157

Following gene: 71910159

Centisome position: 19.11

GC content: 44.09

Gene sequence:

>1998_bases
ATGAAAAAACCATTTTACATTACGACACCCATTTATTACCCATCTGGGAAATTACACATTGGTTCTGCCTATACAACTAT
TGCTTGTGATGTCTTGGCACGCTACAAGCGCCTGATGGGCCATGAGGTCTTTTACCTCACAGGACTAGATGAACACGGAC
AAAAGATTCAAACCAAGGCAAAAGAAGCTGGTATTACACCTCAAACCTACGTTGATAACATGGCAAAGGATGTCAAAGCC
CTTTGGCAATTGCTAGATATTTCCTATGACACATTTATCCGGACAACGGACGATTACCACGAAGAGGTCGTTGCTGCTGT
CTTTGAAAAATTGCTGGCGCAAGATGACATTTACCTTGGCGAATACTCTGGTTGGTATTCTGTCTCTGATGAGGAATTTT
TCACTGAAAGCCAATTAAAAGAAGTCTTTAGAGATGAAGACGGTCAGGTTATTGGAGGGATTGCCCCATCAGGTCATGAG
GTCGAGTGGGTCTCTGAAGAATCCTACTTCCTACGCCTTAGCAAATACGATGATCGTCTTGTGGCCTTCTTTAAAGAGCG
ACCTGACTTTATCCAACCAGATGGTCGTATGAATGAAATGGTGAAAAACTTTATCGAGCCAGGCCTAGAAGATTTGGCTG
TTAGCCGTACTACCTTTACATGGGGTGTACCTGTTCCATCAGACCCAAAACACGTGGTTTATGTCTGGATCGATGCCCTC
TTAAACTACGCGACAGCTCTTGGTTATCGTCAAGCTAATCACGCTAACTTTGATAAGTTTTGGAATGGAACGGTTTTCCA
CATGGTTGGAAAAGATATCTTGCGTTTTCATAGCATCTACTGGCCAATTTTACTCATGATGCTTGATTTACCAATGCCAG
ACCGCTTAATTGCCCATGGTTGGTTTGTCATGAAAGACGGTAAAATGTCTAAATCAAAAGGCAATGTGGTCTATCCAGAA
ATGTTAGTTGAGCGCTTTGGACTTGATCCTCTTCGTTATTACCTCATGCGTTCTTTGCCAGTTGGTTCAGACGGGACCTT
TACCCCTGAAGATTATGTGGGTCGTATCAACTATGAGTTAGCCAACGACCTTGGCAATTTATTGAACCGTACCGTTGCCA
TGATCAACAAGTATTTTGACGGCACGGTGCCAGCTTATGTTGACAATGGAACTGCCTTTGATGCTGACTTAAGTCAACTG
ATTGATGCTCAGCTAGCAGACTACCATAAGCACATGGAAGCGGTGGACTACCCACGCGCCTTGGAGGCTGTTTGGACCAT
CATCGCTCGTACCAACAAATACATCGATGAAACCGCTCCTTGGGTGCTCGCTAAAGAAGATGGTGACAAAGCTCAACTAG
CCAGTGTCATGGCACACTTAGCAGCCAGCTTGCGTCTGGTCGCTCATGTGATCCAACCCTTTATGATGGAAACATCAGCT
GCTATCATGGCCCAGCTTGGTTTGGAACCTGTTTCTGATTTGTCAACACTTGCTCTAGCAGATTTTCCTGCCAATACAAA
GGTTGTTGCCAAAGGGACACCGATTTTCCCACGTCTTGACATGGAAGCTGAGATTGACTACATCAAAGCCCAAATGGGTG
ACAGCTCAGCTATTTCCCAAGAAAAAGAATGGGTCCCTGAAGAGGTTGCGCTCAAATCTGAAAAAGACGTCATCACATTT
GAAACCTTTGATGCTGTGGAAATCCGTGTGGCAGAAGTCAAGGAAGTGTCAAAAGTGGAAGGTTCAGAAAAATTGCTGCG
CTTTAGAGTAGATGCAGGAGACGGGCAAGACCGTCAAATCTTGTCAGGTATTGCCAAATTTTACCCCAACGAACAAGAAT
TAGTGGGTAAAAAACTACAAATTGTTGCTAACCTGAAACCGCGTAAAATGATGAAAAAATACATCAGTCAAGGGATGATT
CTATCAGCAGAGCATGGAGATCAGCTAACGGTCTTAACCGTTGATTCCTCTGTTCCAAATGGAAGTATCATTGGCTGA

Upstream 100 bases:

>100_bases
ATTAGCCTTGAGGGTTGAATTTAAACAGGAAAAAACGAAATCCTACACTATGCATCTCAATCGTACTTTCATTTGAGTAT
GGTTCTGAAAGGAAACTATG

Downstream 100 bases:

>100_bases
TGCTGACCTATTATTCAAAAAACCTAAGCAAGAACATGTGCTTAGGTTTTTTTGATTGCCTTCAAGAAAGACGAATAACG
AGATGGGTGATATGACATCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQL
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETSA
AIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG

Sequences:

>Translated_665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQL
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETSA
AIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG
>Mature_665_residues
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKA
LWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHE
VEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
LNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPE
MLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQL
IDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETSA
AIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITF
ETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMI
LSAEHGDQLTVLTVDSSVPNGSIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=507, Percent_Identity=34.7140039447732, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI14043022, Length=587, Percent_Identity=27.427597955707, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1788432, Length=696, Percent_Identity=27.8735632183908, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=31.25, Blast_Score=251, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17540794, Length=571, Percent_Identity=26.6199649737303, Blast_Score=190, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=29.6875, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=479, Percent_Identity=32.776617954071, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=25.4901960784314, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=33.4669338677355, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24655311, Length=566, Percent_Identity=25.0883392226148, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_STRP1 (Q9A178)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268725.1
- RefSeq:   YP_281708.1
- ProteinModelPortal:   Q9A178
- EnsemblBacteria:   EBSTRT00000000749
- EnsemblBacteria:   EBSTRT00000028790
- GeneID:   3572556
- GeneID:   900674
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0422
- KEGG:   spz:M5005_Spy_0345
- GeneTree:   EBGT00050000027084
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   SPYO160490:SPY0422-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0345-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75132; Mature: 75132

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKA
CCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
KEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEE
EYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYDDRL
CCCCCEECCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEEEECCCHHH
VAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHHH
LNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHG
HHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
WFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYEL
EEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHHH
ANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPRA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETSA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQ
HHHHHHCCCCHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHCH
EKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQI
HHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
LSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPN
HHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCC
GSIIG
CCCCC
>Mature Secondary Structure
MKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKA
CCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
KEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLG
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEE
EYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYDDRL
CCCCCEECCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEEEECCCHHH
VAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDAL
HHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHHH
LNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHG
HHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
WFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYEL
EEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHHH
ANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPRA
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETSA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQ
HHHHHHCCCCHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHCH
EKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQI
HHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCHHHH
LSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPN
HHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCC
GSIIG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296