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The map label for this gene is rpiR [C]

Identifier: 71910033

GI number: 71910033

Start: 224320

End: 225150

Strand: Reverse

Name: rpiR [C]

Synonym: M5005_Spy_0219

Alternate gene names: 71910033

Gene position: 225150-224320 (Counterclockwise)

Preceding gene: 71910048

Following gene: 71910008

Centisome position: 12.25

GC content: 38.39

Gene sequence:

>831_bases
ATGCCTAAAAACCACCGCCTAATTACCAAAATCGAAGCATCATTGGAACATATGACCAGTTTAGAAAAAGGGATTGCTCA
TTTTTTTATTACAACCGATCTGACACCTCAAGAGTTGACCGCATCCGAGATAGTTAAGCGACTACACATCTCTCAAGCCG
CCTTGACTCGATTTGCAAAAAAATGTGGTTTTACTGGTTATCGTGCTTTTGCCTTTGATTACCTTCACAGTCTTCAAGAA
TCACAAGAGACTTTCCAATCTATTCACTTAGAATTGACAAAGCGCGTGCTGATGGACTACGACGCTCTTATTAATAAAAC
CTATGAGCTCGTTAATGAGGAAAAATTACTGAACTTAGCCAAGCTGATCGATTCTTCTGAGCGAGTCTATTTCTTTGGCA
AAGGATCTTCTGGACTAGTAGCAAGAGAAATGAAGCTACGCTTTATGCGACTTGGTTTAATCTGTGATGCTTATTCAGAC
ACTGATGGTTTTACTTGGGCAAATAGCCTCGTCAATGAAAATTGCCTTGTTTTTGGTTTTTCTTTATCTGGCAAAACAAA
CTCTGTCATCACAGCACTTCATCAAGCTAGTCAGCGAGGAGCCAAAACTGTCCTCTTAACAACAGATAATCAAACCGAAT
TTGACGACTCCTTAGATATTATTCCCGTATCATCAACTCACCAACTACATTATGGGAATCGTGTGTCACCACAATTTCCT
CTTCTTATTATGATGGACATTATTTATGCTTACGTTTTAGCGATTGACAAACCGCACAAAGAAAAAATCTTCAAAAACAC
TATTATTGACAAAGAAAATAACGACCTTTAG

Upstream 100 bases:

>100_bases
GTGGGTTGAACAAATCTGTATCACAAAAAGGGTCGCTACCTTCTGTTAGCGATCTATGCTATACTAGTAGGTAGTTAAAC
TGTAAGGAGTGTGCTTTGCT

Downstream 100 bases:

>100_bases
ACTACAATAGCAAAAAGGGCGTTGTTTTCTTTTTAAAGGGTCGTCATTCACCTCCCCCAGTATACTTAAACAAGAACTCT
TCACGTCATATAGACTCACA

Product: RpiR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEIVKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQE
SQETFQSIHLELTKRVLMDYDALINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSLDIIPVSSTHQLHYGNRVSPQFP
LLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL

Sequences:

>Translated_276_residues
MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEIVKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQE
SQETFQSIHLELTKRVLMDYDALINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSLDIIPVSSTHQLHYGNRVSPQFP
LLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
>Mature_275_residues
PKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEIVKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQES
QETFQSIHLELTKRVLMDYDALINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSDT
DGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSLDIIPVSSTHQLHYGNRVSPQFPL
LIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL

Specific function: Regulatory Protein Involved In Rpib Gene Repression. Also Involved In Als Operon Repression. [C]

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI87082369, Length=265, Percent_Identity=23.3962264150943, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01418 HTH_6; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 31380; Mature: 31249

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEIVKRLHISQAALTRFAK
CCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDALINKTYELVNEEKLLNLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSDTDGFTWANSLVNENCLVFGF
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHCCCCEEEEEE
SLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSLDIIPVSSTHQLHYGNRVSPQFP
EECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEECCCCCCCCCCC
LLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEIVKRLHISQAALTRFAK
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDALINKTYELVNEEKLLNLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSDTDGFTWANSLVNENCLVFGF
HHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHCCCCEEEEEE
SLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSLDIIPVSSTHQLHYGNRVSPQFP
EECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEECCCCCCCCCCC
LLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]