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The map label for this gene is dnaN [H]

Identifier: 71909816

GI number: 71909816

Start: 1712

End: 2848

Strand: Direct

Name: dnaN [H]

Synonym: M5005_Spy_0002

Alternate gene names: 71909816

Gene position: 1712-2848 (Clockwise)

Preceding gene: 71909815

Following gene: 71909817

Centisome position: 0.09

GC content: 33.16

Gene sequence:

>1137_bases
ATGATTCAATTTTCAATTAATCGCACATTATTTATTCATGCTTTAAATACAACTAAACGTGCTATTAGCACTAAAAATGC
CATTCCTATTCTTTCATCAATAAAAATTGAAGTCACTTCTACAGGAGTAACTTTAACAGGGTCTAACGGTCAAATATCAA
TTGAAAACACTATTCCTGTAAGTAATGAAAATGCTGGTTTGCTAATTACCTCTCCAGGAGCTATTTTATTAGAAGCTAGT
TTTTTTATTAATATTATTTCAAGTTTGCCAGATATTAGTATAAATGTTAAAGAAATTGAACAACACCAAGTTGTTTTAAC
CAGTGGTAAATCAGAGATTACCTTAAAAGGAAAAGATGTTGACCAGTATCCTCGTCTACAAGAAGTATCAACAGAAAATC
CTTTGATTTTAAAAACAAAATTATTGAAGTCTATTATTGCTGAAACAGCTTTTGCAGCCAGTTTACAAGAAAGTCGTCCT
ATTTTAACAGGAGTTCATATTGTATTAAGTAATCATAAAGATTTTAAAGCAGTAGCGACTGACTCTCATCGTATGAGCCA
ACGTTTAATCACTTTGGACAATACTTCAGCAGATTTTGATGTAGTTATTCCAAGTAAATCTTTGAGAGAATTTTCAGCAG
TATTTACAGATGATATTGAGACCGTTGAGGTATTTTTCTCACCAAGCCAAATCTTGTTCAGAAGTGAACACATTTCTTTT
TATACACGCCTCTTAGAAGGAAATTATCCCGATACAGACCGTTTATTAATGACAGAATTTGAGACGGAGGTTGTTTTCAA
TACCCAATCCCTTCGCCACGCTATGGAACGTGCCTTCTTGATTTCTAATGCTACTCAAAATGGTACTGTTAAGCTTGAGA
TTACTCAAAATCATATTTCAGCTCATGTTAACTCACCTGAGGTTGGTAAGGTAAACGAGGATTTAGATATTGTTAGTCAG
TCTGGTAGTGATTTAACTATCAGCTTCAATCCAACTTACCTTATTGAGTCTTTAAAAGCTATTAAAAGTGAAACAGTAAA
AATTCATTTCTTATCACCAGTTCGACCATTCACCCTAACACCAGGCGATGAGGAAGAAAGTTTTATCCAATTAATTACAC
CAGTACGAACAAACTAA

Upstream 100 bases:

>100_bases
ATTTAGTCTTGGATTCTCTCGTTTATTTAGAGTTATCCACTATATACACAAGACCTACTACTACTACTTATTATTATACT
TATTAAATAAAGGAGTTCTC

Downstream 100 bases:

>100_bases
GTAAGAAAAAGCTCCCTTTTAGGAGTTTTTTTGTTATTATAAATATTAACGATAATATAAGTGGAGAAAAGGGAATGTAT
CAAATTGGATCATTTGTTGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVTLTGSNGQISIENTIPVSNENAGLLITSPGAILLEAS
FFINIISSLPDISINVKEIEQHQVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISF
YTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQ
SGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN

Sequences:

>Translated_378_residues
MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVTLTGSNGQISIENTIPVSNENAGLLITSPGAILLEAS
FFINIISSLPDISINVKEIEQHQVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISF
YTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQ
SGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN
>Mature_378_residues
MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVTLTGSNGQISIENTIPVSNENAGLLITSPGAILLEAS
FFINIISSLPDISINVKEIEQHQVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISF
YTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQ
SGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=347, Percent_Identity=23.6311239193084, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41885; Mature: 41885

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVTLTGSNGQISIENTIPV
CEEEEECCEEEEEEECCHHHHHCCCCCCCEEEEEEEEEEECCEEEECCCCEEEEECCCCC
SNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQHQVVLTSGKSEITLKGKDV
CCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCEEEHEECCCEEEEEECCCCEEEEECCCC
DQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRPILTGVHIVLSNHKDFKAVAT
CCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEC
DSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISF
CCHHHHCEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHH
YTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHIS
HHHHHCCCCCCCCCEEEEEECEEEEEEHHHHHHHHHHEEEEECCCCCCEEEEEEEECEEE
AHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLT
EECCCCCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCEEEC
PGDEEESFIQLITPVRTN
CCCCCCHHHEEECCCCCC
>Mature Secondary Structure
MIQFSINRTLFIHALNTTKRAISTKNAIPILSSIKIEVTSTGVTLTGSNGQISIENTIPV
CEEEEECCEEEEEEECCHHHHHCCCCCCCEEEEEEEEEEECCEEEECCCCEEEEECCCCC
SNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQHQVVLTSGKSEITLKGKDV
CCCCCEEEEECCCCEEEEHHHHHHHHHHCCCCEEEHEECCCEEEEEECCCCEEEEECCCC
DQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRPILTGVHIVLSNHKDFKAVAT
CCCCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEC
DSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIETVEVFFSPSQILFRSEHISF
CCHHHHCEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHH
YTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERAFLISNATQNGTVKLEITQNHIS
HHHHHCCCCCCCCCEEEEEECEEEEEEHHHHHHHHHHEEEEECCCCCCEEEEEEEECEEE
AHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYLIESLKAIKSETVKIHFLSPVRPFTLT
EECCCCCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCEEEC
PGDEEESFIQLITPVRTN
CCCCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]