Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 71908384

GI number: 71908384

Start: 2970651

End: 2971517

Strand: Reverse

Name: braD [H]

Synonym: Daro_2771

Alternate gene names: 71908384

Gene position: 2971517-2970651 (Counterclockwise)

Preceding gene: 71908385

Following gene: 71908383

Centisome position: 66.02

GC content: 60.9

Gene sequence:

>867_bases
ATGGATTTCGCTTCTTTCCTGATCCAGACGTTGAACTCGCTGCAGTACGGTTTGCTCCTCTTTCTGGTGGCAAGCGGTCT
GACGCTGGTGTTCGGCATCATGGGCATCATCAATCTGGCGCACGGCAGTTTTTACATGATCGGCGCCTATCTGGCCTTCG
TGCTGACCAGCACCACCGGCAGCCTGTTTATGGCCATTCTGCTCGGCGTGCCGCTGGCCCTGATCTTCGGGGCCTTCCTC
GAGTGGGCGCTGTTTTCCCATCTCTACAAGCGTGACCACCTTGAGCAGGTGTTGCTGACCTACGGCCTGATTCTCGTTTT
CGAGGAGTTGCGCAGCTTGCTGGTCGGCGACGAGGTCCATGGCATCGACATTCCTGCCATGTTCAATGGCTCGATCCAGC
TCTCCGAGGTGCTGAGCTACCCGGTCTACCGGCTGGTCATCTCGGCCGTCTGCGTCGTGCTCGCCGTGGCGCTGTATTTC
GTCATCCAGCGTACCCGTCTGGGCATGATGATCCGCGCCGGCAACCACGACCGGACCATGGTCGAAGCCCTGGGCATCAA
CATCAACATGATCTACCGCGTCGTCTTCGCCCTCGGTGTCGCGCTGGCCGCGCTGGCCGGCATGCTCGCCTCGCCGATCT
CGTCGGTGTTCCCGAACATGGGCTCGCAGGTGCTGATCATCTCCTTCGTGATTGTCGTCATCGGCGGCATCGGCTCGGTC
TGGGGGGCGCTCGCCGCTGCGCTGATCGTCGGTTTCGCCGACACCTTCGGCAAGGTGCTGCTGCCTGAGCTGTCCGGCAT
GGTGGTCTATATCGTGATGGCCGGTGTGCTCCTGTGGCGCCCCGAAGGCATCTTCAAGAAAGGTTGA

Upstream 100 bases:

>100_bases
TCGGCTTGTCTTCGCGCCTCGGTATTGCGCCGCCACGCCAATTTCTCCCTCTGAATAATTTTTTGGTCTGACGGGCTCAC
CCGTCCGACCGGGGACTCAC

Downstream 100 bases:

>100_bases
GACCATGAACGCACAACTCCTGCTCAAACTGCTCGGGCTGGCGATCCTGCTCTGCGTGCCGCTGACCGGCGAATCGTTCT
ACACCGAGCTGATCGCCAAG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSFYMIGAYLAFVLTSTTGSLFMAILLGVPLALIFGAFL
EWALFSHLYKRDHLEQVLLTYGLILVFEELRSLLVGDEVHGIDIPAMFNGSIQLSEVLSYPVYRLVISAVCVVLAVALYF
VIQRTRLGMMIRAGNHDRTMVEALGININMIYRVVFALGVALAALAGMLASPISSVFPNMGSQVLIISFVIVVIGGIGSV
WGALAAALIVGFADTFGKVLLPELSGMVVYIVMAGVLLWRPEGIFKKG

Sequences:

>Translated_288_residues
MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSFYMIGAYLAFVLTSTTGSLFMAILLGVPLALIFGAFL
EWALFSHLYKRDHLEQVLLTYGLILVFEELRSLLVGDEVHGIDIPAMFNGSIQLSEVLSYPVYRLVISAVCVVLAVALYF
VIQRTRLGMMIRAGNHDRTMVEALGININMIYRVVFALGVALAALAGMLASPISSVFPNMGSQVLIISFVIVVIGGIGSV
WGALAAALIVGFADTFGKVLLPELSGMVVYIVMAGVLLWRPEGIFKKG
>Mature_288_residues
MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSFYMIGAYLAFVLTSTTGSLFMAILLGVPLALIFGAFL
EWALFSHLYKRDHLEQVLLTYGLILVFEELRSLLVGDEVHGIDIPAMFNGSIQLSEVLSYPVYRLVISAVCVVLAVALYF
VIQRTRLGMMIRAGNHDRTMVEALGININMIYRVVFALGVALAALAGMLASPISSVFPNMGSQVLIISFVIVVIGGIGSV
WGALAAALIVGFADTFGKVLLPELSGMVVYIVMAGVLLWRPEGIFKKG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=301, Percent_Identity=30.8970099667774, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31089; Mature: 31089

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSFYMIGAYLAFVLTSTTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SLFMAILLGVPLALIFGAFLEWALFSHLYKRDHLEQVLLTYGLILVFEELRSLLVGDEVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GIDIPAMFNGSIQLSEVLSYPVYRLVISAVCVVLAVALYFVIQRTRLGMMIRAGNHDRTM
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHH
VEALGININMIYRVVFALGVALAALAGMLASPISSVFPNMGSQVLIISFVIVVIGGIGSV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
WGALAAALIVGFADTFGKVLLPELSGMVVYIVMAGVLLWRPEGIFKKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDFASFLIQTLNSLQYGLLLFLVASGLTLVFGIMGIINLAHGSFYMIGAYLAFVLTSTTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SLFMAILLGVPLALIFGAFLEWALFSHLYKRDHLEQVLLTYGLILVFEELRSLLVGDEVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GIDIPAMFNGSIQLSEVLSYPVYRLVISAVCVVLAVALYFVIQRTRLGMMIRAGNHDRTM
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHH
VEALGININMIYRVVFALGVALAALAGMLASPISSVFPNMGSQVLIISFVIVVIGGIGSV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
WGALAAALIVGFADTFGKVLLPELSGMVVYIVMAGVLLWRPEGIFKKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]