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The map label for this gene is prfC

Identifier: 71908343

GI number: 71908343

Start: 2925140

End: 2926714

Strand: Reverse

Name: prfC

Synonym: Daro_2730

Alternate gene names: 71908343

Gene position: 2926714-2925140 (Counterclockwise)

Preceding gene: 71908344

Following gene: 71908342

Centisome position: 65.02

GC content: 61.46

Gene sequence:

>1575_bases
GTGACTCCCCTCGACAACGAAATCTCCCGCCGTCGCACTTTCGCGATCATTTCCCACCCCGACGCCGGTAAAACGACGCT
GACCGAAAAGCTGCTGTGGTTCGGCGGCGCCATTCAGGTGGCCGGCGAAGTTCGCGCCCGCAAGGCATCCCGCCACGCGA
CCTCGGACTGGATGGAGCTGGAAAAGCAGCGCGGCATTTCGGTGACTTCGTCGGTGATGCAGTTCCCCTACCGCGAATGC
ATGATCAACCTGCTCGACACGCCGGGCCACGAGGACTTCTCGGAAGATACCTACCGCACGCTGACCGCCGTCGACTCGGC
CGTCATGGTCATCGACTCGGTCAACGGTGTCGAGGCGCAGACGATCAAGCTGCTGAACGTCTGCCGCATGCGCGACACAC
CGATCCTGACCTTCATCAACAAGCTCGACCGCGAAGGCAAGGAGCCGATCGACCTGCTCGACGAAATCGAGTCGGTGCTC
GGCATCCAGTGCGCGCCGATGACCTGGCCAATCGGCATGGGCAAGCGTTTCCGCGGCGTCTATCACCTCTACGACGATGC
CATTGCCTTCTTTGATCCGCAGGCCGAGAAGGGTACGGCCGAGATCATCCAGGGCCTCGACAATCCGCGCCTCGATGAGT
TGATTGGAACGCAGGCCGACGAGTTGCGCATGGATATCGAACTGGTCCGCGGCGCTTCGCACGCGTTCGATGCCGAAGCC
TATCTCTCGGGCAAGCAGTCGCCGGTCTTCTTCGGCTCGGCGGTCAACAACTTCGGTGTGCAGAGCCTGCTCGACGCTGT
GGTCGACCTGTCGCCGCCGCCGATTGCCCGTCCTTCGGTCAGCCGCGAGGTGTTGCCGAACGAGCCGAAGTTCTCCGGTT
TCGTATTCAAGATTCAGGCCAACATGGACCCCAAGCACCGGGACCGTATCGCCTTCCTGCGCGTCTGTTCCGGCCGCTTT
GACAGGGGTATGAAGGTCAAGCAGGTGGCTTCCGGCAAGATGCTGTCAATCAACAATGCCATTACCTTCATGGCCCGCGA
CCGTTCCACGACCGACGAGGCCTGGCCGGGCGACATCATCGGTATTCCGAACCACGGCACGATCCGCCTGGGCGAAACCT
TTACCGAAGGCGAAGATCTGCGCTTTACCGGCATCCCGTCCTTCGCGCCCGAGCATTTCCGTCTGGCCCGCATCGCCAAC
CCGCTGAAGATCAAGCAATTGCAAAAGGGCTTGCAGCAGCTGGCGGAAGAGGGCGCCACCCAGTTGTTCCGGCCGCTGTC
CGGTACCGACCTGATCCTTGGGGCGGTCGGTACGCTGCAGTTCGACGTGGTGGCCAGTCGTCTGGAAAATGAATACGGCG
TGCAGGTCATCTTCGAGCACTACAACTGCGCGACGGCGCGCTGGATTCACGGTGATGCCGCCGAGTTGCGCCAGTTGTCC
GACCGCTACAGTGCCAACGTCGCGTTGGATGGGGCTGACGACCCGGTCTATCTGGCACCGAACAATGTCTATCTGAACAT
GGTCAAAGAGAAGTACCCCAACCTGCGCTTCCTCGAAGCGCGCGAAGTGGCGTAA

Upstream 100 bases:

>100_bases
CCGGGCACTCTACCGCGAAGCACTGCCGCTCGCCTGAACGCCGGTGCTTGCCTAGCGGCAAAGGTATAATTGCTGCCTTT
CCGCATTTCCAAGCCCTGCC

Downstream 100 bases:

>100_bases
GAAGGGGGCTGAGCATGACTGTCCGGGTTCAGGGAGCGGATTTCGATGTTGGTGCCGAACTGGCCGCATTGCGCGCCGGG
GATGCCCGCGTCGGGGCGCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTPLDNEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKASRHATSDWMELEKQRGISVTSSVMQFPYREC
MINLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVL
GIQCAPMTWPIGMGKRFRGVYHLYDDAIAFFDPQAEKGTAEIIQGLDNPRLDELIGTQADELRMDIELVRGASHAFDAEA
YLSGKQSPVFFGSAVNNFGVQSLLDAVVDLSPPPIARPSVSREVLPNEPKFSGFVFKIQANMDPKHRDRIAFLRVCSGRF
DRGMKVKQVASGKMLSINNAITFMARDRSTTDEAWPGDIIGIPNHGTIRLGETFTEGEDLRFTGIPSFAPEHFRLARIAN
PLKIKQLQKGLQQLAEEGATQLFRPLSGTDLILGAVGTLQFDVVASRLENEYGVQVIFEHYNCATARWIHGDAAELRQLS
DRYSANVALDGADDPVYLAPNNVYLNMVKEKYPNLRFLEAREVA

Sequences:

>Translated_524_residues
MTPLDNEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKASRHATSDWMELEKQRGISVTSSVMQFPYREC
MINLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVL
GIQCAPMTWPIGMGKRFRGVYHLYDDAIAFFDPQAEKGTAEIIQGLDNPRLDELIGTQADELRMDIELVRGASHAFDAEA
YLSGKQSPVFFGSAVNNFGVQSLLDAVVDLSPPPIARPSVSREVLPNEPKFSGFVFKIQANMDPKHRDRIAFLRVCSGRF
DRGMKVKQVASGKMLSINNAITFMARDRSTTDEAWPGDIIGIPNHGTIRLGETFTEGEDLRFTGIPSFAPEHFRLARIAN
PLKIKQLQKGLQQLAEEGATQLFRPLSGTDLILGAVGTLQFDVVASRLENEYGVQVIFEHYNCATARWIHGDAAELRQLS
DRYSANVALDGADDPVYLAPNNVYLNMVKEKYPNLRFLEAREVA
>Mature_523_residues
TPLDNEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKASRHATSDWMELEKQRGISVTSSVMQFPYRECM
INLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVLG
IQCAPMTWPIGMGKRFRGVYHLYDDAIAFFDPQAEKGTAEIIQGLDNPRLDELIGTQADELRMDIELVRGASHAFDAEAY
LSGKQSPVFFGSAVNNFGVQSLLDAVVDLSPPPIARPSVSREVLPNEPKFSGFVFKIQANMDPKHRDRIAFLRVCSGRFD
RGMKVKQVASGKMLSINNAITFMARDRSTTDEAWPGDIIGIPNHGTIRLGETFTEGEDLRFTGIPSFAPEHFRLARIANP
LKIKQLQKGLQQLAEEGATQLFRPLSGTDLILGAVGTLQFDVVASRLENEYGVQVIFEHYNCATARWIHGDAAELRQLSD
RYSANVALDGADDPVYLAPNNVYLNMVKEKYPNLRFLEAREVA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=487, Percent_Identity=29.1581108829569, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=25.9842519685039, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI25306283, Length=350, Percent_Identity=28.2857142857143, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI25306287, Length=479, Percent_Identity=26.3048016701461, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=29.7619047619048, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=33.1081081081081, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310132016, Length=126, Percent_Identity=34.9206349206349, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=126, Percent_Identity=34.9206349206349, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=126, Percent_Identity=34.9206349206349, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790835, Length=528, Percent_Identity=57.5757575757576, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=484, Percent_Identity=29.1322314049587, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.974358974359, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.2281879194631, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=495, Percent_Identity=28.4848484848485, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=34.8314606741573, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=35.6687898089172, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=503, Percent_Identity=28.8270377733598, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=375, Percent_Identity=28.8, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324707, Length=563, Percent_Identity=23.0905861456483, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=563, Percent_Identity=23.0905861456483, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=147, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324166, Length=223, Percent_Identity=28.6995515695067, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=490, Percent_Identity=29.1836734693878, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=26.0700389105058, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.9622641509434, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_DECAR (Q47CH1)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285930.1
- ProteinModelPortal:   Q47CH1
- SMR:   Q47CH1
- STRING:   Q47CH1
- GeneID:   3566879
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2730
- NMPDR:   fig|159087.4.peg.3714
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   HERTVEP
- ProtClustDB:   PRK00741
- BioCyc:   DARO159087:DARO_2730-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 58395; Mature: 58264

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLDNEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKASRHATSDWMEL
CCCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHH
EKQRGISVTSSVMQFPYRECMINLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQ
HHHCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHH
TIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVLGIQCAPMTWPIGMGKRFRGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHH
YHLYDDAIAFFDPQAEKGTAEIIQGLDNPRLDELIGTQADELRMDIELVRGASHAFDAEA
HHHHHCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
YLSGKQSPVFFGSAVNNFGVQSLLDAVVDLSPPPIARPSVSREVLPNEPKFSGFVFKIQA
HHCCCCCCEEECCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
NMDPKHRDRIAFLRVCSGRFDRGMKVKQVASGKMLSINNAITFMARDRSTTDEAWPGDII
CCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCEE
GIPNHGTIRLGETFTEGEDLRFTGIPSFAPEHFRLARIANPLKIKQLQKGLQQLAEEGAT
ECCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QLFRPLSGTDLILGAVGTLQFDVVASRLENEYGVQVIFEHYNCATARWIHGDAAELRQLS
HHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCHHHHHHHH
DRYSANVALDGADDPVYLAPNNVYLNMVKEKYPNLRFLEAREVA
HHHCCCEEECCCCCCEEECCCCEEEEEHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
TPLDNEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKASRHATSDWMEL
CCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHH
EKQRGISVTSSVMQFPYRECMINLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQ
HHHCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHH
TIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVLGIQCAPMTWPIGMGKRFRGV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHH
YHLYDDAIAFFDPQAEKGTAEIIQGLDNPRLDELIGTQADELRMDIELVRGASHAFDAEA
HHHHHCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
YLSGKQSPVFFGSAVNNFGVQSLLDAVVDLSPPPIARPSVSREVLPNEPKFSGFVFKIQA
HHCCCCCCEEECCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
NMDPKHRDRIAFLRVCSGRFDRGMKVKQVASGKMLSINNAITFMARDRSTTDEAWPGDII
CCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCEE
GIPNHGTIRLGETFTEGEDLRFTGIPSFAPEHFRLARIANPLKIKQLQKGLQQLAEEGAT
ECCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QLFRPLSGTDLILGAVGTLQFDVVASRLENEYGVQVIFEHYNCATARWIHGDAAELRQLS
HHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCHHHHHHHH
DRYSANVALDGADDPVYLAPNNVYLNMVKEKYPNLRFLEAREVA
HHHCCCEEECCCCCCEEECCCCEEEEEHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA