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The map label for this gene is gdhA [H]

Identifier: 71908334

GI number: 71908334

Start: 2915269

End: 2916552

Strand: Reverse

Name: gdhA [H]

Synonym: Daro_2721

Alternate gene names: 71908334

Gene position: 2916552-2915269 (Counterclockwise)

Preceding gene: 71908336

Following gene: 71908333

Centisome position: 64.8

GC content: 60.9

Gene sequence:

>1284_bases
ATGAATGCCCCGCTGCTGCCTTCTTATATTTCGGCTCAAAACATCGGTCCGTGGCACATCTTCCTGCAGCAAGTCGAGCA
GGTCGCACCGCTGCTCGACAAGTCGCTGTGGCCGTGGGTGGAAACCCTGCGTCGACCAAAACGTTCGTTGATCGTCGATG
TGCCGATCCGTCTCGATAGCGGTGAAGTGGCGCATTTTGAGGGTTATCGCGTGCATCACAACACCTCACGCGGGCCGGGC
AAGGGCGGCGTGCGCTTTCATCAGGATGTCACCTTGTCCGAGGTGATGGCGCTGGCCGGCTGGATGACGATCAAGAACGC
GGTAGTCAATGTGCCGTTCGGCGGTGCCAAGGGCGGTGTCCGTGTCGATCCCCGGCAGCTTTCGATTTCGGAACTCGAAG
GGCTGACCCGCCGTTACACCAGCGAAATCAGTTCGATGATCGGGCCGGACAAGGATATTCCGGCGCCGGACATGAATACC
AATGCCCAGGTCATGGCCTGGATGATGGATACCTATTCCATGGGTGAGGGGCGGACGGTGACTGGCGTCGTGACCGGCAA
GCCGCTGTCGCTGGGCGGTTCGCTCGGGCGGCAGGATGCAACCGGGCGGGGCGTTTTTGTCACTGCCCGCGAGGCAGCAC
GCAAGCTGAATCTGCCGATTGAGGGAGCGCGGGTGGCTGTGCAGGGCTTCGGTAATGTCGGCGAGGCTAGTGCGCGGATC
TTTGCGCAGGCCGGCGCCAGGATCGTTGCCGTGCAGGATGTCAGTGCGACGCTTTATTGCGAAGCGGGTCTGGATATCGC
GGCCTTGAAGCGGTATTTGGCGGAAAACAAGACATTGCTTGGCGCACCGGGTTGCGAAGTGATTGATAACGCGGCTTTCT
GGGCTGTGCCTTGCGACTTCATGGTGCCGGCCGCACTGGAGTCGCAGATCAACCGCTATAACGCCGGCCAGATTACCGCC
CGTATTGTCGTCGAAGGTGCCAATGGCCCGACCACCCCTGAGGCCGATGTCATCCTGGCCGAGCGCGGCATCACCGTTGT
GCCCGACGTGCTGGCCAATGCCGGGGGCGTCACGGTCAGTTACTTCGAGTGGGTGCAGGATTTTTCCAGCTTCTTCTGGA
GTGAGGATGAGATCTACCAGCGCCTTGAACGGATCATGTCCGAGGCCTTCAATGCGATCTGGCAACTGGCAGAGGCCCGC
CAGATGTCGCTCCGTTCGGCTGCCTTCGTGATTGGATGCACTCGGGTGCTCGAAGCCCGGGCGACGCGTGGTTTGTATCC
CTGA

Upstream 100 bases:

>100_bases
AATGTTGTGGGCAGAGCATACCGCCAAGCCGTGAAGGCGCGCTAGTCAAGTACGCTTTGATGCGCCACACTGAGCGTCAT
CCAGCCTGCAAGGAGTTGCC

Downstream 100 bases:

>100_bases
TATGGCTGCGGCGAGGCTTTTAGCCTGCGGTTGGACCGCATTCTATGGCGGCGCCTTCGGTATTGGCGGGTACTATCTGC
CTTTGCCCATTTAAGAGAAG

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal:Glu/Leu/Phe/Val dehydrogenase, dimerization region

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNAPLLPSYISAQNIGPWHIFLQQVEQVAPLLDKSLWPWVETLRRPKRSLIVDVPIRLDSGEVAHFEGYRVHHNTSRGPG
KGGVRFHQDVTLSEVMALAGWMTIKNAVVNVPFGGAKGGVRVDPRQLSISELEGLTRRYTSEISSMIGPDKDIPAPDMNT
NAQVMAWMMDTYSMGEGRTVTGVVTGKPLSLGGSLGRQDATGRGVFVTAREAARKLNLPIEGARVAVQGFGNVGEASARI
FAQAGARIVAVQDVSATLYCEAGLDIAALKRYLAENKTLLGAPGCEVIDNAAFWAVPCDFMVPAALESQINRYNAGQITA
RIVVEGANGPTTPEADVILAERGITVVPDVLANAGGVTVSYFEWVQDFSSFFWSEDEIYQRLERIMSEAFNAIWQLAEAR
QMSLRSAAFVIGCTRVLEARATRGLYP

Sequences:

>Translated_427_residues
MNAPLLPSYISAQNIGPWHIFLQQVEQVAPLLDKSLWPWVETLRRPKRSLIVDVPIRLDSGEVAHFEGYRVHHNTSRGPG
KGGVRFHQDVTLSEVMALAGWMTIKNAVVNVPFGGAKGGVRVDPRQLSISELEGLTRRYTSEISSMIGPDKDIPAPDMNT
NAQVMAWMMDTYSMGEGRTVTGVVTGKPLSLGGSLGRQDATGRGVFVTAREAARKLNLPIEGARVAVQGFGNVGEASARI
FAQAGARIVAVQDVSATLYCEAGLDIAALKRYLAENKTLLGAPGCEVIDNAAFWAVPCDFMVPAALESQINRYNAGQITA
RIVVEGANGPTTPEADVILAERGITVVPDVLANAGGVTVSYFEWVQDFSSFFWSEDEIYQRLERIMSEAFNAIWQLAEAR
QMSLRSAAFVIGCTRVLEARATRGLYP
>Mature_427_residues
MNAPLLPSYISAQNIGPWHIFLQQVEQVAPLLDKSLWPWVETLRRPKRSLIVDVPIRLDSGEVAHFEGYRVHHNTSRGPG
KGGVRFHQDVTLSEVMALAGWMTIKNAVVNVPFGGAKGGVRVDPRQLSISELEGLTRRYTSEISSMIGPDKDIPAPDMNT
NAQVMAWMMDTYSMGEGRTVTGVVTGKPLSLGGSLGRQDATGRGVFVTAREAARKLNLPIEGARVAVQGFGNVGEASARI
FAQAGARIVAVQDVSATLYCEAGLDIAALKRYLAENKTLLGAPGCEVIDNAAFWAVPCDFMVPAALESQINRYNAGQITA
RIVVEGANGPTTPEADVILAERGITVVPDVLANAGGVTVSYFEWVQDFSSFFWSEDEIYQRLERIMSEAFNAIWQLAEAR
QMSLRSAAFVIGCTRVLEARATRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=353, Percent_Identity=42.7762039660057, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4885281, Length=353, Percent_Identity=42.4929178470255, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI1788059, Length=431, Percent_Identity=30.3944315545244, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=341, Percent_Identity=40.7624633431085, Blast_Score=257, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6324951, Length=415, Percent_Identity=28.9156626506024, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319256, Length=417, Percent_Identity=29.7362110311751, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553475, Length=364, Percent_Identity=40.6593406593407, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI45549226, Length=374, Percent_Identity=40.1069518716578, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24649283, Length=338, Percent_Identity=41.1242603550296, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649460, Length=314, Percent_Identity=40.1273885350318, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46367; Mature: 46367

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLLPSYISAQNIGPWHIFLQQVEQVAPLLDKSLWPWVETLRRPKRSLIVDVPIRLDS
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECC
GEVAHFEGYRVHHNTSRGPGKGGVRFHQDVTLSEVMALAGWMTIKNAVVNVPFGGAKGGV
CCEEEECCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RVDPRQLSISELEGLTRRYTSEISSMIGPDKDIPAPDMNTNAQVMAWMMDTYSMGEGRTV
EECHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEHHHHCCCCCCEE
TGVVTGKPLSLGGSLGRQDATGRGVFVTAREAARKLNLPIEGARVAVQGFGNVGEASARI
EEEEECCCEECCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCEEEEECCCCCCHHHHHH
FAQAGARIVAVQDVSATLYCEAGLDIAALKRYLAENKTLLGAPGCEVIDNAAFWAVPCDF
HHHCCCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHCCCEEEEECCHH
MVPAALESQINRYNAGQITARIVVEGANGPTTPEADVILAERGITVVPDVLANAGGVTVS
HCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCEEECHHHCCCCCCCHH
YFEWVQDFSSFFWSEDEIYQRLERIMSEAFNAIWQLAEARQMSLRSAAFVIGCTRVLEAR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRGLYP
HHCCCCC
>Mature Secondary Structure
MNAPLLPSYISAQNIGPWHIFLQQVEQVAPLLDKSLWPWVETLRRPKRSLIVDVPIRLDS
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEEECC
GEVAHFEGYRVHHNTSRGPGKGGVRFHQDVTLSEVMALAGWMTIKNAVVNVPFGGAKGGV
CCEEEECCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RVDPRQLSISELEGLTRRYTSEISSMIGPDKDIPAPDMNTNAQVMAWMMDTYSMGEGRTV
EECHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEHHHHCCCCCCEE
TGVVTGKPLSLGGSLGRQDATGRGVFVTAREAARKLNLPIEGARVAVQGFGNVGEASARI
EEEEECCCEECCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCEEEEECCCCCCHHHHHH
FAQAGARIVAVQDVSATLYCEAGLDIAALKRYLAENKTLLGAPGCEVIDNAAFWAVPCDF
HHHCCCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHCCCEEEEECCHH
MVPAALESQINRYNAGQITARIVVEGANGPTTPEADVILAERGITVVPDVLANAGGVTVS
HCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCEEECHHHCCCCCCCHH
YFEWVQDFSSFFWSEDEIYQRLERIMSEAFNAIWQLAEARQMSLRSAAFVIGCTRVLEAR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATRGLYP
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]