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The map label for this gene is intB [H]

Identifier: 71908317

GI number: 71908317

Start: 2896523

End: 2897737

Strand: Reverse

Name: intB [H]

Synonym: Daro_2704

Alternate gene names: 71908317

Gene position: 2897737-2896523 (Counterclockwise)

Preceding gene: 71908326

Following gene: 71908315

Centisome position: 64.38

GC content: 54.81

Gene sequence:

>1215_bases
ATGCCGTTGAGTAACACTGCAATCATCAATGCCAAGCCTTCGGACAAGCCCTATGCGCTGACCGACGAACGCGGGCTGTC
TATTCAGATTCAACCAACTGGCGGGAAGTGGTGGCGCTTGCGTTATCGCTTCGACGGCAAGGCAAAGATGCTCTCTTTGG
GAGTATATCCTGACGTCGGCTTGAAAGAGGCTAGGGAGCGCCGCGAAGAGGCTCGCAAACTACTGGCCACAGGTGTTGAT
CCCGGAGAGCATCGAAAAGCACACAAGGCTGCAAAGGCAGAACGTTCAGCCAACAGTTTTGAAGTGATTTGCCGTGAATG
GCTGGAGCAGCGCAAAGATACAGTCGAGCCAGCACAGACCGCGAAGACCTTGGCCCGGATGGAAAACGATGTTTTCCCTT
GGATTGGTGGCAAAGCGATAAGCGAGATCACCGCTCCCGATGTATTGAGTGTGATGCGCCGAATGGATGAGCGGGGCGCC
AGATACACCGCCCACAGGGTACGGAGTGAAATCAGCCGGGCATTCCGATATGCAATTGCTACCGGCCGCGCCGAGCGCGA
TCCTTGTCCTGACCTTCGCGGGGCGATCCCGCCAGCCAAAGAAACACATTTTGCTGCGATTACCGACCCAAAAGAAGTCG
GAGGACTTCTAAGGGCGTTTGATGGCTTCAGCGGCACGTTCGTGGTCAAGTCGGCGCTACTCCTTGCACCGTTACTTTTC
ACTCGCCCTGGTGAGCTACGTAAAGCAGAGTGGGCAAGTTTTGATCTTGAGAAAAGCGACTGGCGCTATTTCATCAACAA
GACGAAGACCGAGCACCTAGTGCCACTGGCAACTCAGGCAGTCGTGATCCTCAGAGACTTGCATGCACTGACCGGGCATG
GCCGTTATCTGTTTCCGAATGCACGAACCAGTGAAAGACCAATGAGCGAGGCTGCGGTGAATGCGGCGCTACGCCGTATT
GGCTATGACACCAAAACAGAAATTTCAGGTCATGGCTTTCGTGCAATGGCTCGGACGATTCTGCATGAAGAACTGCACGT
GAAGCCGGAAGTGATCGAACACCAATTGGCACACACCGTACCCGATGCCCTTGGTACTGCCTACAACCGCACCAAGTTTC
TCAAGGAGCGCAAGGCGATGATGCAGCTATGGGCGGACTATCTGGATAAGCTGAAAGCAGGGGCGGAAGTCATACCGTTT
CATGGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GGTTTTTGTCCGGTTGTGTGCAACATGGTCCAACAATATCCAGTCTCCAGTGTGTATAACTCTGTGAGTAACTGGGTGTG
AGCCAGAGGGAGTTACACAC

Downstream 100 bases:

>100_bases
AATAAAAAAGCCGGGAGTTACCCCGGCCAGCCTGTCCTAAATTTTCAGGGTAGTTACTCTTGTGCGTTCATCGCCGCAGC
CCATTCAACAATCTGTTGCT

Product: Phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPLSNTAIINAKPSDKPYALTDERGLSIQIQPTGGKWWRLRYRFDGKAKMLSLGVYPDVGLKEARERREEARKLLATGVD
PGEHRKAHKAAKAERSANSFEVICREWLEQRKDTVEPAQTAKTLARMENDVFPWIGGKAISEITAPDVLSVMRRMDERGA
RYTAHRVRSEISRAFRYAIATGRAERDPCPDLRGAIPPAKETHFAAITDPKEVGGLLRAFDGFSGTFVVKSALLLAPLLF
TRPGELRKAEWASFDLEKSDWRYFINKTKTEHLVPLATQAVVILRDLHALTGHGRYLFPNARTSERPMSEAAVNAALRRI
GYDTKTEISGHGFRAMARTILHEELHVKPEVIEHQLAHTVPDALGTAYNRTKFLKERKAMMQLWADYLDKLKAGAEVIPF
HGAA

Sequences:

>Translated_404_residues
MPLSNTAIINAKPSDKPYALTDERGLSIQIQPTGGKWWRLRYRFDGKAKMLSLGVYPDVGLKEARERREEARKLLATGVD
PGEHRKAHKAAKAERSANSFEVICREWLEQRKDTVEPAQTAKTLARMENDVFPWIGGKAISEITAPDVLSVMRRMDERGA
RYTAHRVRSEISRAFRYAIATGRAERDPCPDLRGAIPPAKETHFAAITDPKEVGGLLRAFDGFSGTFVVKSALLLAPLLF
TRPGELRKAEWASFDLEKSDWRYFINKTKTEHLVPLATQAVVILRDLHALTGHGRYLFPNARTSERPMSEAAVNAALRRI
GYDTKTEISGHGFRAMARTILHEELHVKPEVIEHQLAHTVPDALGTAYNRTKFLKERKAMMQLWADYLDKLKAGAEVIPF
HGAA
>Mature_403_residues
PLSNTAIINAKPSDKPYALTDERGLSIQIQPTGGKWWRLRYRFDGKAKMLSLGVYPDVGLKEARERREEARKLLATGVDP
GEHRKAHKAAKAERSANSFEVICREWLEQRKDTVEPAQTAKTLARMENDVFPWIGGKAISEITAPDVLSVMRRMDERGAR
YTAHRVRSEISRAFRYAIATGRAERDPCPDLRGAIPPAKETHFAAITDPKEVGGLLRAFDGFSGTFVVKSALLLAPLLFT
RPGELRKAEWASFDLEKSDWRYFINKTKTEHLVPLATQAVVILRDLHALTGHGRYLFPNARTSERPMSEAAVNAALRRIG
YDTKTEISGHGFRAMARTILHEELHVKPEVIEHQLAHTVPDALGTAYNRTKFLKERKAMMQLWADYLDKLKAGAEVIPFH
GAA

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=397, Percent_Identity=35.7682619647355, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788690, Length=393, Percent_Identity=36.3867684478371, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788974, Length=401, Percent_Identity=32.1695760598504, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45414; Mature: 45282

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSNTAIINAKPSDKPYALTDERGLSIQIQPTGGKWWRLRYRFDGKAKMLSLGVYPDVG
CCCCCCEEEECCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCCCC
LKEARERREEARKLLATGVDPGEHRKAHKAAKAERSANSFEVICREWLEQRKDTVEPAQT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHH
AKTLARMENDVFPWIGGKAISEITAPDVLSVMRRMDERGARYTAHRVRSEISRAFRYAIA
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TGRAERDPCPDLRGAIPPAKETHFAAITDPKEVGGLLRAFDGFSGTFVVKSALLLAPLLF
HCCCCCCCCCCHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TRPGELRKAEWASFDLEKSDWRYFINKTKTEHLVPLATQAVVILRDLHALTGHGRYLFPN
CCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCC
ARTSERPMSEAAVNAALRRIGYDTKTEISGHGFRAMARTILHEELHVKPEVIEHQLAHTV
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PDALGTAYNRTKFLKERKAMMQLWADYLDKLKAGAEVIPFHGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure 
PLSNTAIINAKPSDKPYALTDERGLSIQIQPTGGKWWRLRYRFDGKAKMLSLGVYPDVG
CCCCCEEEECCCCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCCCC
LKEARERREEARKLLATGVDPGEHRKAHKAAKAERSANSFEVICREWLEQRKDTVEPAQT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHH
AKTLARMENDVFPWIGGKAISEITAPDVLSVMRRMDERGARYTAHRVRSEISRAFRYAIA
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TGRAERDPCPDLRGAIPPAKETHFAAITDPKEVGGLLRAFDGFSGTFVVKSALLLAPLLF
HCCCCCCCCCCHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TRPGELRKAEWASFDLEKSDWRYFINKTKTEHLVPLATQAVVILRDLHALTGHGRYLFPN
CCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCC
ARTSERPMSEAAVNAALRRIGYDTKTEISGHGFRAMARTILHEELHVKPEVIEHQLAHTV
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PDALGTAYNRTKFLKERKAMMQLWADYLDKLKAGAEVIPFHGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]