Click here to switch to the map view.

The map label for this gene is kinA [H]

Identifier: 71908214

GI number: 71908214

Start: 2801178

End: 2802632

Strand: Reverse

Name: kinA [H]

Synonym: Daro_2601

Alternate gene names: 71908214

Gene position: 2802632-2801178 (Counterclockwise)

Preceding gene: 71908215

Following gene: 71908213

Centisome position: 62.27

GC content: 59.93

Gene sequence:

>1455_bases
ATGATCTTTCGCCGTAACCTTTCGTTGCGCTGGAAAATCCCGCTACGGGTGATGGCGGCAGTGCTGGGCACGGCAATTAC
CGTAACGGCGGCACTGGTTGTCTGGGAATACGACGAGATGCGGCAAAACCTGGAAGGCCATGCCAAGAGTCTCGGGCGGG
TATTGGCCAATACCCTGGTCGCGCCGGTGCTCCATGACGACATCTGGCGAGCCTACGAAATTCTGCAGTCAGCGCGTGAA
ACCTATCCGGCCGCTCCCGAGTTGCAAGCCGAGGTGGTCCTTATCACCGACGCCGATTACAAGGTTTTCGTATCGACGAA
GCCTCGCGAATTTCCGATCGGCAGCAGTCCAGCCACTCGCGGCGGCGCCTATGCGCAGTTGCGTACCGGCATGGTCAGCG
GTGACGGGATGGCGCAGCGTGTCCTCGAACCGGCTGATTCCCCGGTCTACTTCGTGATGTCGCCGCTGGTTGCCGATGGG
GTGGCACTCGGTCATGTCATTCTTGGCTACTCCAAGGTTTCTTTCGTGCCGCGCTTCGTCGGCTTGGTCAAGCGGGCGGC
GCTGGTCACCGTACTGGTGCTGCTGATCATTGCGCCGATTTCCTGGGTCTGGGCCAGTCGCACCGGGGAGCCGTTGCTTC
GTCTGGCTGAAGCTATGCGCCGCGTGCCGGGCGAGCTTGAGGAGTCTCGCCTGACAGACCTACCAAAAAGCGGCGACGAG
ATTGGCCAACTGGCAGCCGCTTATCAGCGCATGGTCTCTGAGCTAAAAAACAAGCAGGAACTGGAGCGGCAAATGCTCGT
CTCCGAACGGCTCGCTGCCGTCGGCCGCCTGACGGCCGGCATCGCCCACGAAATCAACAATCCGCTGGGCGGCATGCTGA
CCGCCATCAACACCTACCAGCGGCACGGCAGCGGTAACGATCCGCTTGCTCTGAGCACGCTGTCGCTGCTCGAACGCGGC
CTGACGCAGATTCGCCATACGGTGGGAGCACTGCTCGTCGAAAGCAAGGCCAAGGATCGCCCCTTCGCTTCCGAGGATGT
GGATGATCTGCTGATCCTGGCCGAGTCCGAAGCACATGGGCGTGGTATCGGCGTCGTCGTCGAAAGCAATCTCGATGGCG
CATTGCCTCTGCCGGCAACCCTGATGCGCCAGATTCTACTTAACCTGCTACTCAATGCCTTGGCTGCAGCCGAGGAGGGT
GGCGGCGTGCGTTTGATCGTCGAGTCAGCCGAAAAGCAATTGCACCTGGCTGTGACCAACGATGGTGACTACATTCCGCA
GGAACGCATGGATACCCTGTTCGAGCCTTTCGTTACGACCAGCGAGCGCGGCAATGGCCTGGGTTTGTGGATCGTTTATC
AGATCGTTCAGCAACTGGGTGGCGAAATCGAGGTCGAGAGCCTGCCCGGCTACACTACTTTCAAGGTTGATATTCCTTAT
GGCAACACCGTCTGA

Upstream 100 bases:

>100_bases
GGGGCGAATTGCTGGGCCGCTTGCGACTGGATGGCTTCGTGAGCGGATCGCCAGCCTTGTTCGATGGCATTGCCCAGATG
GCAGCCAAGGTCGGCCGGAA

Downstream 100 bases:

>100_bases
AATCCGCCCACATCTTTGCCTGATCGAGGACGACGAGATCATGGGCGAGTCGCTTTCCCAGCGCTTCCAGTTAGAAGGCT
TCGATGTCGATTGGTGGCGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIFRRNLSLRWKIPLRVMAAVLGTAITVTAALVVWEYDEMRQNLEGHAKSLGRVLANTLVAPVLHDDIWRAYEILQSARE
TYPAAPELQAEVVLITDADYKVFVSTKPREFPIGSSPATRGGAYAQLRTGMVSGDGMAQRVLEPADSPVYFVMSPLVADG
VALGHVILGYSKVSFVPRFVGLVKRAALVTVLVLLIIAPISWVWASRTGEPLLRLAEAMRRVPGELEESRLTDLPKSGDE
IGQLAAAYQRMVSELKNKQELERQMLVSERLAAVGRLTAGIAHEINNPLGGMLTAINTYQRHGSGNDPLALSTLSLLERG
LTQIRHTVGALLVESKAKDRPFASEDVDDLLILAESEAHGRGIGVVVESNLDGALPLPATLMRQILLNLLLNALAAAEEG
GGVRLIVESAEKQLHLAVTNDGDYIPQERMDTLFEPFVTTSERGNGLGLWIVYQIVQQLGGEIEVESLPGYTTFKVDIPY
GNTV

Sequences:

>Translated_484_residues
MIFRRNLSLRWKIPLRVMAAVLGTAITVTAALVVWEYDEMRQNLEGHAKSLGRVLANTLVAPVLHDDIWRAYEILQSARE
TYPAAPELQAEVVLITDADYKVFVSTKPREFPIGSSPATRGGAYAQLRTGMVSGDGMAQRVLEPADSPVYFVMSPLVADG
VALGHVILGYSKVSFVPRFVGLVKRAALVTVLVLLIIAPISWVWASRTGEPLLRLAEAMRRVPGELEESRLTDLPKSGDE
IGQLAAAYQRMVSELKNKQELERQMLVSERLAAVGRLTAGIAHEINNPLGGMLTAINTYQRHGSGNDPLALSTLSLLERG
LTQIRHTVGALLVESKAKDRPFASEDVDDLLILAESEAHGRGIGVVVESNLDGALPLPATLMRQILLNLLLNALAAAEEG
GGVRLIVESAEKQLHLAVTNDGDYIPQERMDTLFEPFVTTSERGNGLGLWIVYQIVQQLGGEIEVESLPGYTTFKVDIPY
GNTV
>Mature_484_residues
MIFRRNLSLRWKIPLRVMAAVLGTAITVTAALVVWEYDEMRQNLEGHAKSLGRVLANTLVAPVLHDDIWRAYEILQSARE
TYPAAPELQAEVVLITDADYKVFVSTKPREFPIGSSPATRGGAYAQLRTGMVSGDGMAQRVLEPADSPVYFVMSPLVADG
VALGHVILGYSKVSFVPRFVGLVKRAALVTVLVLLIIAPISWVWASRTGEPLLRLAEAMRRVPGELEESRLTDLPKSGDE
IGQLAAAYQRMVSELKNKQELERQMLVSERLAAVGRLTAGIAHEINNPLGGMLTAINTYQRHGSGNDPLALSTLSLLERG
LTQIRHTVGALLVESKAKDRPFASEDVDDLLILAESEAHGRGIGVVVESNLDGALPLPATLMRQILLNLLLNALAAAEEG
GGVRLIVESAEKQLHLAVTNDGDYIPQERMDTLFEPFVTTSERGNGLGLWIVYQIVQQLGGEIEVESLPGYTTFKVDIPY
GNTV

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=251, Percent_Identity=30.6772908366534, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=30.672268907563, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790300, Length=259, Percent_Identity=30.1158301158301, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790567, Length=96, Percent_Identity=40.625, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52724; Mature: 52724

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFRRNLSLRWKIPLRVMAAVLGTAITVTAALVVWEYDEMRQNLEGHAKSLGRVLANTLV
CEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
APVLHDDIWRAYEILQSARETYPAAPELQAEVVLITDADYKVFVSTKPREFPIGSSPATR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCC
GGAYAQLRTGMVSGDGMAQRVLEPADSPVYFVMSPLVADGVALGHVILGYSKVSFVPRFV
CCCHHHHHHCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVKRAALVTVLVLLIIAPISWVWASRTGEPLLRLAEAMRRVPGELEESRLTDLPKSGDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHH
IGQLAAAYQRMVSELKNKQELERQMLVSERLAAVGRLTAGIAHEINNPLGGMLTAINTYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RHGSGNDPLALSTLSLLERGLTQIRHTVGALLVESKAKDRPFASEDVDDLLILAESEAHG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
RGIGVVVESNLDGALPLPATLMRQILLNLLLNALAAAEEGGGVRLIVESAEKQLHLAVTN
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEC
DGDYIPQERMDTLFEPFVTTSERGNGLGLWIVYQIVQQLGGEIEVESLPGYTTFKVDIPY
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCC
GNTV
CCCC
>Mature Secondary Structure
MIFRRNLSLRWKIPLRVMAAVLGTAITVTAALVVWEYDEMRQNLEGHAKSLGRVLANTLV
CEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
APVLHDDIWRAYEILQSARETYPAAPELQAEVVLITDADYKVFVSTKPREFPIGSSPATR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCC
GGAYAQLRTGMVSGDGMAQRVLEPADSPVYFVMSPLVADGVALGHVILGYSKVSFVPRFV
CCCHHHHHHCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVKRAALVTVLVLLIIAPISWVWASRTGEPLLRLAEAMRRVPGELEESRLTDLPKSGDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHH
IGQLAAAYQRMVSELKNKQELERQMLVSERLAAVGRLTAGIAHEINNPLGGMLTAINTYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RHGSGNDPLALSTLSLLERGLTQIRHTVGALLVESKAKDRPFASEDVDDLLILAESEAHG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCC
RGIGVVVESNLDGALPLPATLMRQILLNLLLNALAAAEEGGGVRLIVESAEKQLHLAVTN
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEC
DGDYIPQERMDTLFEPFVTTSERGNGLGLWIVYQIVQQLGGEIEVESLPGYTTFKVDIPY
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCC
GNTV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]