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The map label for this gene is nusA [H]

Identifier: 71908071

GI number: 71908071

Start: 2654472

End: 2655944

Strand: Reverse

Name: nusA [H]

Synonym: Daro_2453

Alternate gene names: 71908071

Gene position: 2655944-2654472 (Counterclockwise)

Preceding gene: 71908072

Following gene: 71908070

Centisome position: 59.01

GC content: 54.72

Gene sequence:

>1473_bases
ATGAGCCGTGAAATTTTGCTGCTGGTCGATGCTCTGGCCCGCGAAAAAAATGTCAGCAAGGAGATCGTCTTTGGCGCCCT
TGAGCTGGCACTCGCGTCAGCGACCAAAAAGCGCATCAATGACGAGGCCGATGTTCGTATCTCAATCGATCGCGACACTG
GTAGCTTCGAATCATTCCGTCGCTGGCAGGTTGTGCCGGATAACGAATACGTCAACGAATTCCTCGAAATTCCGTTGTCC
GATGCCCAGAAGGATGATCCTGAAATCGAGCCCGGCGACTCTCTGGAAGAGCCGCTTGAGCCGATCGATTTTGGTCGTAT
CGGTGCTCAGGCTGCCAAACAGGTTATTTTGCAGAAGATCCGCGACGCCGAGCGCGAGCAGATTCTGGCTGACTTTCTTG
GTCGCGGCGAGCATGTCGTTTCCGGTACCATCAAGCGCATGGAGCGTGGCAACGCCATTATTGAGGCTGGTAAAATTGAA
GCCATGCTGCCGCGCGACCAGATGATCCCCAAGGAAAATCTGCGTGTCGGCGACAGAGTTCGTGCCTATTTGCTGCGCAT
TGATCGCAATGCCCGTGGTCCGCAAATCATCCTTTCGCGCACCGCTCCGGAATTTGTCATCAAGCTTTTTGACATGGAAG
TGCCGGAAATTTCCGATGGCCTGATGGAACTCAAGGCCTGTGCCCGTGACCCCGGTCTGCGCGCCAAAATCGCTGTCAAG
TCGAACGATCCCCGTGTTGATCCAATCGGTACCTGCGTCGGTTTGCGCGGTTCCCGGGTTACCGCCGTTCGTAACGAAAT
CGGTGGCGAGAATATCGACATCGTGCTGTGGTCAGCCGATCCGGCGCAATTCGTTATCGGCGCGTTGTCGCCAGCTGAAG
TGTCCTCCATCGTGGTTGATGAAGAAAAGCACGCAATGGATGTCGTGGTCGACGAGGATAACCTTGCGATCGCCATCGGT
CGCAACGGGCAGAACGTTCGCCTGGCCTCCGAACTGACTGGCTGGACCATCAATCTGATGACGCAGGACGAGTCGGCCAA
GAAATCCGAAGCCGAATTCGCCGAGACGCGCGTCGTCTTTATGGAAAAGCTGGATATCGATGAAGAACTTGCCGATCTGC
TGATCGAGGAAGGGTTCTCGACGCTGGAAGAAGTGGCCTACGTGCCGCTGGCAGAAATGCTGGAAATCGAAGGTCTGGAT
GAGGAAATCGTAAATGAGTTGCGTAATCGGGCTCGTAACGTCCTGCTCACCGAGGCTATCGCAACTGAAGAAAAGCTGGA
AAGTGTTTCCGAAGACCTGATTGGTCTCGAAGGCATGAGCAAGGAACTGGCCGCCAAACTGGCTGGACACGATGTCAAAA
CCCGGGATGATCTCGCGGAATTGGCTGTTGATGAATTGACGGAAATGACCGGCATTGACGATGAGCGTGCCAAGGATCTT
ATCCTGAAGGCACGGGCTCACTGGTTCGAGTGA

Upstream 100 bases:

>100_bases
TTTGCGCCTGGCAAAAGATGATGTGGAACTGGAATTCACCAATATCGAGAAGGCACGTCTGGTGCCTCGTTTCGACTGAA
GGACTAGGAGGTTTTAGCCC

Downstream 100 bases:

>100_bases
GCGGGAGGTAGCAAATATGTCCGCAACAACTGTTTCACAATTTGCCGTTGAACTCAAAATGCCGGTTGTGGCCTTGCTCG
AGCAATTGGGCAAGGCTGGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSREILLLVDALAREKNVSKEIVFGALELALASATKKRINDEADVRISIDRDTGSFESFRRWQVVPDNEYVNEFLEIPLS
DAQKDDPEIEPGDSLEEPLEPIDFGRIGAQAAKQVILQKIRDAEREQILADFLGRGEHVVSGTIKRMERGNAIIEAGKIE
AMLPRDQMIPKENLRVGDRVRAYLLRIDRNARGPQIILSRTAPEFVIKLFDMEVPEISDGLMELKACARDPGLRAKIAVK
SNDPRVDPIGTCVGLRGSRVTAVRNEIGGENIDIVLWSADPAQFVIGALSPAEVSSIVVDEEKHAMDVVVDEDNLAIAIG
RNGQNVRLASELTGWTINLMTQDESAKKSEAEFAETRVVFMEKLDIDEELADLLIEEGFSTLEEVAYVPLAEMLEIEGLD
EEIVNELRNRARNVLLTEAIATEEKLESVSEDLIGLEGMSKELAAKLAGHDVKTRDDLAELAVDELTEMTGIDDERAKDL
ILKARAHWFE

Sequences:

>Translated_490_residues
MSREILLLVDALAREKNVSKEIVFGALELALASATKKRINDEADVRISIDRDTGSFESFRRWQVVPDNEYVNEFLEIPLS
DAQKDDPEIEPGDSLEEPLEPIDFGRIGAQAAKQVILQKIRDAEREQILADFLGRGEHVVSGTIKRMERGNAIIEAGKIE
AMLPRDQMIPKENLRVGDRVRAYLLRIDRNARGPQIILSRTAPEFVIKLFDMEVPEISDGLMELKACARDPGLRAKIAVK
SNDPRVDPIGTCVGLRGSRVTAVRNEIGGENIDIVLWSADPAQFVIGALSPAEVSSIVVDEEKHAMDVVVDEDNLAIAIG
RNGQNVRLASELTGWTINLMTQDESAKKSEAEFAETRVVFMEKLDIDEELADLLIEEGFSTLEEVAYVPLAEMLEIEGLD
EEIVNELRNRARNVLLTEAIATEEKLESVSEDLIGLEGMSKELAAKLAGHDVKTRDDLAELAVDELTEMTGIDDERAKDL
ILKARAHWFE
>Mature_489_residues
SREILLLVDALAREKNVSKEIVFGALELALASATKKRINDEADVRISIDRDTGSFESFRRWQVVPDNEYVNEFLEIPLSD
AQKDDPEIEPGDSLEEPLEPIDFGRIGAQAAKQVILQKIRDAEREQILADFLGRGEHVVSGTIKRMERGNAIIEAGKIEA
MLPRDQMIPKENLRVGDRVRAYLLRIDRNARGPQIILSRTAPEFVIKLFDMEVPEISDGLMELKACARDPGLRAKIAVKS
NDPRVDPIGTCVGLRGSRVTAVRNEIGGENIDIVLWSADPAQFVIGALSPAEVSSIVVDEEKHAMDVVVDEDNLAIAIGR
NGQNVRLASELTGWTINLMTQDESAKKSEAEFAETRVVFMEKLDIDEELADLLIEEGFSTLEEVAYVPLAEMLEIEGLDE
EIVNELRNRARNVLLTEAIATEEKLESVSEDLIGLEGMSKELAAKLAGHDVKTRDDLAELAVDELTEMTGIDDERAKDLI
LKARAHWFE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=493, Percent_Identity=51.3184584178499, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54485; Mature: 54353

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILLLVDALAREKNVSKEIVFGALELALASATKKRINDEADVRISIDRDTGSFESFR
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWQVVPDNEYVNEFLEIPLSDAQKDDPEIEPGDSLEEPLEPIDFGRIGAQAAKQVILQKI
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
RDAEREQILADFLGRGEHVVSGTIKRMERGNAIIEAGKIEAMLPRDQMIPKENLRVGDRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCEEEECCHHHCCCCCCCCHHHHH
RAYLLRIDRNARGPQIILSRTAPEFVIKLFDMEVPEISDGLMELKACARDPGLRAKIAVK
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
SNDPRVDPIGTCVGLRGSRVTAVRNEIGGENIDIVLWSADPAQFVIGALSPAEVSSIVVD
CCCCCCCCHHHEECCCCCEEHHHHHHCCCCCEEEEEECCCHHHHHEECCCHHHHHHEEEC
EEKHAMDVVVDEDNLAIAIGRNGQNVRLASELTGWTINLMTQDESAKKSEAEFAETRVVF
CCCCCEEEEEECCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHH
MEKLDIDEELADLLIEEGFSTLEEVAYVPLAEMLEIEGLDEEIVNELRNRARNVLLTEAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHEEHHHHH
ATEEKLESVSEDLIGLEGMSKELAAKLAGHDVKTRDDLAELAVDELTEMTGIDDERAKDL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ILKARAHWFE
HHHHHHHCCC
>Mature Secondary Structure 
SREILLLVDALAREKNVSKEIVFGALELALASATKKRINDEADVRISIDRDTGSFESFR
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RWQVVPDNEYVNEFLEIPLSDAQKDDPEIEPGDSLEEPLEPIDFGRIGAQAAKQVILQKI
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
RDAEREQILADFLGRGEHVVSGTIKRMERGNAIIEAGKIEAMLPRDQMIPKENLRVGDRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCEEEECCHHHCCCCCCCCHHHHH
RAYLLRIDRNARGPQIILSRTAPEFVIKLFDMEVPEISDGLMELKACARDPGLRAKIAVK
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
SNDPRVDPIGTCVGLRGSRVTAVRNEIGGENIDIVLWSADPAQFVIGALSPAEVSSIVVD
CCCCCCCCHHHEECCCCCEEHHHHHHCCCCCEEEEEECCCHHHHHEECCCHHHHHHEEEC
EEKHAMDVVVDEDNLAIAIGRNGQNVRLASELTGWTINLMTQDESAKKSEAEFAETRVVF
CCCCCEEEEEECCCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHH
MEKLDIDEELADLLIEEGFSTLEEVAYVPLAEMLEIEGLDEEIVNELRNRARNVLLTEAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHEEHHHHH
ATEEKLESVSEDLIGLEGMSKELAAKLAGHDVKTRDDLAELAVDELTEMTGIDDERAKDL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
ILKARAHWFE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]