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The map label for this gene is ykfC [H]

Identifier: 71907870

GI number: 71907870

Start: 2433628

End: 2434917

Strand: Reverse

Name: ykfC [H]

Synonym: Daro_2250

Alternate gene names: 71907870

Gene position: 2434917-2433628 (Counterclockwise)

Preceding gene: 71907871

Following gene: 71907867

Centisome position: 54.1

GC content: 56.82

Gene sequence:

>1290_bases
ATGGCGTTACACGCGAAAGCGAAGTCGGAATCTGGATATCGTTTCTACGCGCTGTACGACAAGATTTATCGCACGGATAT
TTTGGCGCATGCCTATGCCCAGTGCCGCTCCAATAAGGGCGCGCCGGGTGTGGATCGTCAGGATTTCGAGGATGTCGAGG
CGTATGGTGTGCGGCGATGGCTGGAGGAACTGGCGCTTGCGCTCAAAGAGGAGAGCTACCGACCGGATCCAATTCGGAGA
GTGTTTATCCCGAAAGCCAATGGCAAGTTAAGGCCTCTGGGCATTTCAACGCTGCATGATCGAGTGTGTATGACAGCAGC
CATGCTGGTACTCGAACCTATCTTTGAAGCTGATCTTCCTGATGAACAGTATGCCTACCGGCCGGGCCGCAATGCCCAGC
AGGCGGCAGAAGAAGTGAAGAACCGGCTCTACCTTGGACAAACGGACGTTGTCGATGCCGACCTGTCGGACTACTTCGGC
AGCATTCCACATTCTGAACTGATGAAGTCGCTGGCGCGACGCATCGTGGATCGGCGTGTGCTACATCTTATCAAGATGTG
GCTGGAGTGCGCGGTTGAGGAAACCGATCAGCGAGGACGGAAGAAACGGACGACCGAGGCCAAAGATCAGGGGCGAGGTA
TCCCGCAAGGCTCACCGATCTCGCCGCTGCTATCGAATTTGTATATGCGGCGGTTTGTGCTGGCGTGGAAGAAACTGGGG
TTGGAGCGAAGCCTTGGCAGTCGCATCGTCACCTATGCCGACGACCTCGTGATCCTGTGCAAGTGTGGCAAGGCGGAAGA
AGCCTTGCAATGGATGCGCACGATCATGGGGAAACTGAAGCTCACGGTGAACGAGGAAAAGACACGAATCTGTCAGGTAC
CGGCAGGGACGTTCGACTTTCTGGGTTACTCGTTTGGACGGCGATATGTGCCGCGCACAGGGAAGCCGCAGATCGCTCTG
TGGCCGTCGAAGAAAAGCATTCGACGCATGGTGGAGAAAATCCATGACATGACTGAGCGGCAAACGGGTTGGCAAGAGAC
CACGGAGCTGGTGGGCAAGTTGAATCGGACGCTACGCGGCTGGGCGAACTACTTCAGTGTAGGGAACGTCAGTCGCGCCT
ATCGTGCGCTCGACAGTTACACGGCAACGCGGTTGCGTCGGTGGTTGCGCTACAAGTACAAGCTCAGACATTGCAAGGGC
GGGAGCTATCCACTCTCGCACCTCTACGGGTACTTTGGTCTCGTACGTCTGGGCGCACGTGGGCGCAGCGAGGCGTGGGT
GAAGGCGTGA

Upstream 100 bases:

>100_bases
GGTGGAGGGAAGGGGCCTTGGTTCATGACAGACGCACTACGTAGTGAGGACGTGGAGATTGGGCAACCTATCAACTCCGT
TGCATGTTCAGAAACTGCAG

Downstream 100 bases:

>100_bases
TGTCGTGTCCGAGAGCCGGATGCGGGAAATCTGCATGTCCGGTTCGATGAGGGGGATGTGAAAACGGGGTTACGGCAGAG
TTACTTGGGCACCGCCAGAC

Product: RNA-directed DNA polymerase (Reverse transcriptase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MALHAKAKSESGYRFYALYDKIYRTDILAHAYAQCRSNKGAPGVDRQDFEDVEAYGVRRWLEELALALKEESYRPDPIRR
VFIPKANGKLRPLGISTLHDRVCMTAAMLVLEPIFEADLPDEQYAYRPGRNAQQAAEEVKNRLYLGQTDVVDADLSDYFG
SIPHSELMKSLARRIVDRRVLHLIKMWLECAVEETDQRGRKKRTTEAKDQGRGIPQGSPISPLLSNLYMRRFVLAWKKLG
LERSLGSRIVTYADDLVILCKCGKAEEALQWMRTIMGKLKLTVNEEKTRICQVPAGTFDFLGYSFGRRYVPRTGKPQIAL
WPSKKSIRRMVEKIHDMTERQTGWQETTELVGKLNRTLRGWANYFSVGNVSRAYRALDSYTATRLRRWLRYKYKLRHCKG
GSYPLSHLYGYFGLVRLGARGRSEAWVKA

Sequences:

>Translated_429_residues
MALHAKAKSESGYRFYALYDKIYRTDILAHAYAQCRSNKGAPGVDRQDFEDVEAYGVRRWLEELALALKEESYRPDPIRR
VFIPKANGKLRPLGISTLHDRVCMTAAMLVLEPIFEADLPDEQYAYRPGRNAQQAAEEVKNRLYLGQTDVVDADLSDYFG
SIPHSELMKSLARRIVDRRVLHLIKMWLECAVEETDQRGRKKRTTEAKDQGRGIPQGSPISPLLSNLYMRRFVLAWKKLG
LERSLGSRIVTYADDLVILCKCGKAEEALQWMRTIMGKLKLTVNEEKTRICQVPAGTFDFLGYSFGRRYVPRTGKPQIAL
WPSKKSIRRMVEKIHDMTERQTGWQETTELVGKLNRTLRGWANYFSVGNVSRAYRALDSYTATRLRRWLRYKYKLRHCKG
GSYPLSHLYGYFGLVRLGARGRSEAWVKA
>Mature_428_residues
ALHAKAKSESGYRFYALYDKIYRTDILAHAYAQCRSNKGAPGVDRQDFEDVEAYGVRRWLEELALALKEESYRPDPIRRV
FIPKANGKLRPLGISTLHDRVCMTAAMLVLEPIFEADLPDEQYAYRPGRNAQQAAEEVKNRLYLGQTDVVDADLSDYFGS
IPHSELMKSLARRIVDRRVLHLIKMWLECAVEETDQRGRKKRTTEAKDQGRGIPQGSPISPLLSNLYMRRFVLAWKKLGL
ERSLGSRIVTYADDLVILCKCGKAEEALQWMRTIMGKLKLTVNEEKTRICQVPAGTFDFLGYSFGRRYVPRTGKPQIALW
PSKKSIRRMVEKIHDMTERQTGWQETTELVGKLNRTLRGWANYFSVGNVSRAYRALDSYTATRLRRWLRYKYKLRHCKGG
SYPLSHLYGYFGLVRLGARGRSEAWVKA

Specific function: Unknown

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226520, Length=356, Percent_Identity=25, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6226521, Length=209, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015706
- InterPro:   IPR000477 [H]

Pfam domain/function: PF00078 RVT_1 [H]

EC number: NA

Molecular weight: Translated: 49448; Mature: 49317

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHAKAKSESGYRFYALYDKIYRTDILAHAYAQCRSNKGAPGVDRQDFEDVEAYGVRRW
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LEELALALKEESYRPDPIRRVFIPKANGKLRPLGISTLHDRVCMTAAMLVLEPIFEADLP
HHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DEQYAYRPGRNAQQAAEEVKNRLYLGQTDVVDADLSDYFGSIPHSELMKSLARRIVDRRV
CCHHCCCCCCCHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LHLIKMWLECAVEETDQRGRKKRTTEAKDQGRGIPQGSPISPLLSNLYMRRFVLAWKKLG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LERSLGSRIVTYADDLVILCKCGKAEEALQWMRTIMGKLKLTVNEEKTRICQVPAGTFDF
HHHHHCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHH
LGYSFGRRYVPRTGKPQIALWPSKKSIRRMVEKIHDMTERQTGWQETTELVGKLNRTLRG
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
WANYFSVGNVSRAYRALDSYTATRLRRWLRYKYKLRHCKGGSYPLSHLYGYFGLVRLGAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
GRSEAWVKA
CCCCCCCCC
>Mature Secondary Structure 
ALHAKAKSESGYRFYALYDKIYRTDILAHAYAQCRSNKGAPGVDRQDFEDVEAYGVRRW
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LEELALALKEESYRPDPIRRVFIPKANGKLRPLGISTLHDRVCMTAAMLVLEPIFEADLP
HHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DEQYAYRPGRNAQQAAEEVKNRLYLGQTDVVDADLSDYFGSIPHSELMKSLARRIVDRRV
CCHHCCCCCCCHHHHHHHHHHHEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LHLIKMWLECAVEETDQRGRKKRTTEAKDQGRGIPQGSPISPLLSNLYMRRFVLAWKKLG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LERSLGSRIVTYADDLVILCKCGKAEEALQWMRTIMGKLKLTVNEEKTRICQVPAGTFDF
HHHHHCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCHHHH
LGYSFGRRYVPRTGKPQIALWPSKKSIRRMVEKIHDMTERQTGWQETTELVGKLNRTLRG
HHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
WANYFSVGNVSRAYRALDSYTATRLRRWLRYKYKLRHCKGGSYPLSHLYGYFGLVRLGAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
GRSEAWVKA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]