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The map label for this gene is gacS [H]

Identifier: 71907731

GI number: 71907731

Start: 2266119

End: 2268161

Strand: Reverse

Name: gacS [H]

Synonym: Daro_2105

Alternate gene names: 71907731

Gene position: 2268161-2266119 (Counterclockwise)

Preceding gene: 71907734

Following gene: 71907720

Centisome position: 50.39

GC content: 53.7

Gene sequence:

>2043_bases
ATGAGCAATGCGGAAAACGACCTTTCGAACGAACGATTGCATGATTTTTCTCGCAGTTCTGCTGACTGGTTTTGGGAAAC
GGATACTGATCACCGGTTTACCTATCTGTCCGATGATATTGAGTCTAAAGTCGGCGTCCCTATTGACTACTTGATGGGGA
AAAGCCGCAGGGAACTGGCCACGCAACTCACTAACAATCCTCCCGGAGACTGGGAGAAGTACTTGGCCGCCTTGAATGCG
CGAGAAGCTTTTCGGGATTTTGAATACTGCGCCTTAGCGCCGAATGGCGTGGCCTATTGGTTCAGCATCAGCGGCGTCCC
GTTCTTTGACGAACAGGGGGAATTTCGTGGCTATCGGGGGGTCGGGCGTGATGTCACCGAAACGCACTCGATGCAGGTTG
AGCTGCACCGTTACCGGAGCCACCTTGAAGACGAGGTCCGAAGGCGCACGGAAGAATCATTGAAGGAGCGTGAACGGGCA
ATATCGGCGTCGCAGGCAAAGTCGATTTTTCTCGCCAACATGAGCCATGAAATTCGTACGCCGATGAATGCGATCGTCGG
ACTGACGCATTTGTTGCGCAAAGAGATCACGACGCCCGCACAGCTTGAAAAGCTCGCCCAGGTTTCTGCGTCTGCTGACC
ATTTGTTATCGGTCATCAACGATATTCTCGATATCTCAAAAATCGAGTCCGGCAATGCCGCCCTGGACGAACTGGATTTT
GAGCTGGAAGGCATGATCCGGCGGGTCAGCAGCGTCATCGCACTGCGCGCCCAGGCCAAGGGACTGGAACTCATCGTCGA
TATCAGAACGCTGCCGACTGTCCTGCATGGCGACCCCACCCGACTGAGCCAGGTCCTGATCAATTTTCTTGGCAATGCAG
TCAAATTTACCGTGCAGGGGTCGATTACCTTGCGCGGCCGGATAGAGGCCGAAACGGCAACAGATATGGTTGTCCGATTC
GAAGTCGAAGACACCGGCATCGGCATTTCGCCTGACGCACAAGAAAAAATCTTTGAGGCGTTTGAACAGGCGGATCAATC
AACCACCCGCAACTATGGCGGAACGGGATTGGGACTGGCGATTGCCAAGCATGCGGCGAAAATGATGAAAGGCGATGTCG
GGGTACGCAGCACGCCTGGCGTCGGCAGCGTATTCTGGATTACCGCCAGACTCGGGAAAGTCAATGCAGCCGGCCCGGAG
TTCATTGTCCCGGAAGCGGTAGGCGTATCCACTTTGGTGGTAGACGACTTGCCGTTAACGCAGGCGGTCCACTCTCAATT
GCTCAAACGCATCGGCCTCAACCCGATAGCGGTGATGTCCGGTCAAGAAGCTTTAACCGCTGTGCAGATTGCCGACCGCG
AACTGCATCCGTTTGGGATTGCCTTTATTGACCTTCACATGCCCGACTTGAATGGCCTGGAAGTCATTGCGAAAATCCGA
GCCTTGCCCTTGCGATACCAGCCGCATTGCGTTCTGGTCACTGCCGCTGGTATTCAAAGCATTGTCGACGAAGCCAAGTC
AGTCGGATATGCGGCAGTTTTGCAGAAACCAGCCAGCTTGGCTGGCCTGCGTGAAATTGTCAGCCAGCTTCTGACAACAC
AGGGAAGCCAATTTCAATATCTGAAAGAAAAAAAACGTCCTTGCGAAGCCTTGCTTCGGGAACACAAAGCCGGTTGCCGC
ATTCTGCTGGTTGAGGATGAACCAATCAATCAAATGATCGCCCGCGAATTGCTCGAAGATGCTTCGCTGGTTGTCCACGT
GGCGGACAACGGCCAACAGGCGGTTGAAATGGCGCTATCAACGCCTTCTCCTTATGACCTTATCCTGATGGACGTACAGA
TGCCGGTTTTGGACGGCATTGCCGCTGCCCACTGTATCCGCGAAAAGCGCCCGGATCTCCATACGCCCATCATTGCGCTG
ACGGCAAATGCGTTTTCTGATGACCGCCGCAAATGTCTGGCTGCAGGGATGAATGATTTTGTCTCCAAACCCGTTGATCC
GGATGTTCTATTCGAGATCATCTGGAAATGGCTGTCAAAATAG

Upstream 100 bases:

>100_bases
AATAATTCATATCTATTTTTATTGTAAAAATCATTAGAATCGCTGAATCTGATTTGTGAAACCCTCATCGCCACAACACT
TTGACGAGGCATTCCCTGCA

Downstream 100 bases:

>100_bases
GTACAGGTGTTCGTTTGAGCGCTGCCCGCCAATATTTTGTCGTCCTTGCCCACTCGTTACTGAACGTCAGGAAAGCAGTG
AATGGCTGCCTGATTAGCAG

Product: PAS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MSNAENDLSNERLHDFSRSSADWFWETDTDHRFTYLSDDIESKVGVPIDYLMGKSRRELATQLTNNPPGDWEKYLAALNA
REAFRDFEYCALAPNGVAYWFSISGVPFFDEQGEFRGYRGVGRDVTETHSMQVELHRYRSHLEDEVRRRTEESLKERERA
ISASQAKSIFLANMSHEIRTPMNAIVGLTHLLRKEITTPAQLEKLAQVSASADHLLSVINDILDISKIESGNAALDELDF
ELEGMIRRVSSVIALRAQAKGLELIVDIRTLPTVLHGDPTRLSQVLINFLGNAVKFTVQGSITLRGRIEAETATDMVVRF
EVEDTGIGISPDAQEKIFEAFEQADQSTTRNYGGTGLGLAIAKHAAKMMKGDVGVRSTPGVGSVFWITARLGKVNAAGPE
FIVPEAVGVSTLVVDDLPLTQAVHSQLLKRIGLNPIAVMSGQEALTAVQIADRELHPFGIAFIDLHMPDLNGLEVIAKIR
ALPLRYQPHCVLVTAAGIQSIVDEAKSVGYAAVLQKPASLAGLREIVSQLLTTQGSQFQYLKEKKRPCEALLREHKAGCR
ILLVEDEPINQMIARELLEDASLVVHVADNGQQAVEMALSTPSPYDLILMDVQMPVLDGIAAAHCIREKRPDLHTPIIAL
TANAFSDDRRKCLAAGMNDFVSKPVDPDVLFEIIWKWLSK

Sequences:

>Translated_680_residues
MSNAENDLSNERLHDFSRSSADWFWETDTDHRFTYLSDDIESKVGVPIDYLMGKSRRELATQLTNNPPGDWEKYLAALNA
REAFRDFEYCALAPNGVAYWFSISGVPFFDEQGEFRGYRGVGRDVTETHSMQVELHRYRSHLEDEVRRRTEESLKERERA
ISASQAKSIFLANMSHEIRTPMNAIVGLTHLLRKEITTPAQLEKLAQVSASADHLLSVINDILDISKIESGNAALDELDF
ELEGMIRRVSSVIALRAQAKGLELIVDIRTLPTVLHGDPTRLSQVLINFLGNAVKFTVQGSITLRGRIEAETATDMVVRF
EVEDTGIGISPDAQEKIFEAFEQADQSTTRNYGGTGLGLAIAKHAAKMMKGDVGVRSTPGVGSVFWITARLGKVNAAGPE
FIVPEAVGVSTLVVDDLPLTQAVHSQLLKRIGLNPIAVMSGQEALTAVQIADRELHPFGIAFIDLHMPDLNGLEVIAKIR
ALPLRYQPHCVLVTAAGIQSIVDEAKSVGYAAVLQKPASLAGLREIVSQLLTTQGSQFQYLKEKKRPCEALLREHKAGCR
ILLVEDEPINQMIARELLEDASLVVHVADNGQQAVEMALSTPSPYDLILMDVQMPVLDGIAAAHCIREKRPDLHTPIIAL
TANAFSDDRRKCLAAGMNDFVSKPVDPDVLFEIIWKWLSK
>Mature_679_residues
SNAENDLSNERLHDFSRSSADWFWETDTDHRFTYLSDDIESKVGVPIDYLMGKSRRELATQLTNNPPGDWEKYLAALNAR
EAFRDFEYCALAPNGVAYWFSISGVPFFDEQGEFRGYRGVGRDVTETHSMQVELHRYRSHLEDEVRRRTEESLKERERAI
SASQAKSIFLANMSHEIRTPMNAIVGLTHLLRKEITTPAQLEKLAQVSASADHLLSVINDILDISKIESGNAALDELDFE
LEGMIRRVSSVIALRAQAKGLELIVDIRTLPTVLHGDPTRLSQVLINFLGNAVKFTVQGSITLRGRIEAETATDMVVRFE
VEDTGIGISPDAQEKIFEAFEQADQSTTRNYGGTGLGLAIAKHAAKMMKGDVGVRSTPGVGSVFWITARLGKVNAAGPEF
IVPEAVGVSTLVVDDLPLTQAVHSQLLKRIGLNPIAVMSGQEALTAVQIADRELHPFGIAFIDLHMPDLNGLEVIAKIRA
LPLRYQPHCVLVTAAGIQSIVDEAKSVGYAAVLQKPASLAGLREIVSQLLTTQGSQFQYLKEKKRPCEALLREHKAGCRI
LLVEDEPINQMIARELLEDASLVVHVADNGQQAVEMALSTPSPYDLILMDVQMPVLDGIAAAHCIREKRPDLHTPIIALT
ANAFSDDRRKCLAAGMNDFVSKPVDPDVLFEIIWKWLSK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=567, Percent_Identity=32.6278659611993, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI87081816, Length=439, Percent_Identity=35.3075170842825, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI48994928, Length=566, Percent_Identity=31.095406360424, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI145693157, Length=537, Percent_Identity=31.6573556797021, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788713, Length=448, Percent_Identity=29.0178571428571, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=31.7596566523605, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786600, Length=254, Percent_Identity=28.740157480315, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=29.4605809128631, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=27.6679841897233, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082128, Length=222, Percent_Identity=29.2792792792793, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1790436, Length=266, Percent_Identity=27.8195488721804, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788393, Length=221, Percent_Identity=28.0542986425339, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=27.2340425531915, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790861, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787894, Length=225, Percent_Identity=29.3333333333333, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=78, Percent_Identity=50, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322000, Length=123, Percent_Identity=35.7723577235772, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323034, Length=144, Percent_Identity=34.7222222222222, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75175; Mature: 75044

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS00086 CYTOCHROME_P450 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAENDLSNERLHDFSRSSADWFWETDTDHRFTYLSDDIESKVGVPIDYLMGKSRRELA
CCCCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHCCCCCHHHHHCCHHHHHH
TQLTNNPPGDWEKYLAALNAREAFRDFEYCALAPNGVAYWFSISGVPFFDEQGEFRGYRG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCC
VGRDVTETHSMQVELHRYRSHLEDEVRRRTEESLKERERAISASQAKSIFLANMSHEIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHC
PMNAIVGLTHLLRKEITTPAQLEKLAQVSASADHLLSVINDILDISKIESGNAALDELDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCH
ELEGMIRRVSSVIALRAQAKGLELIVDIRTLPTVLHGDPTRLSQVLINFLGNAVKFTVQG
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEE
SITLRGRIEAETATDMVVRFEVEDTGIGISPDAQEKIFEAFEQADQSTTRNYGGTGLGLA
EEEEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IAKHAAKMMKGDVGVRSTPGVGSVFWITARLGKVNAAGPEFIVPEAVGVSTLVVDDLPLT
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCHH
QAVHSQLLKRIGLNPIAVMSGQEALTAVQIADRELHPFGIAFIDLHMPDLNGLEVIAKIR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
ALPLRYQPHCVLVTAAGIQSIVDEAKSVGYAAVLQKPASLAGLREIVSQLLTTQGSQFQY
HCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
LKEKKRPCEALLREHKAGCRILLVEDEPINQMIARELLEDASLVVHVADNGQQAVEMALS
HHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHC
TPSPYDLILMDVQMPVLDGIAAAHCIREKRPDLHTPIIALTANAFSDDRRKCLAAGMNDF
CCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCHHHH
VSKPVDPDVLFEIIWKWLSK
HCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNAENDLSNERLHDFSRSSADWFWETDTDHRFTYLSDDIESKVGVPIDYLMGKSRRELA
CCCCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHCCCCCHHHHHCCHHHHHH
TQLTNNPPGDWEKYLAALNAREAFRDFEYCALAPNGVAYWFSISGVPFFDEQGEFRGYRG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCC
VGRDVTETHSMQVELHRYRSHLEDEVRRRTEESLKERERAISASQAKSIFLANMSHEIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHC
PMNAIVGLTHLLRKEITTPAQLEKLAQVSASADHLLSVINDILDISKIESGNAALDELDF
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCH
ELEGMIRRVSSVIALRAQAKGLELIVDIRTLPTVLHGDPTRLSQVLINFLGNAVKFTVQG
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEE
SITLRGRIEAETATDMVVRFEVEDTGIGISPDAQEKIFEAFEQADQSTTRNYGGTGLGLA
EEEEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IAKHAAKMMKGDVGVRSTPGVGSVFWITARLGKVNAAGPEFIVPEAVGVSTLVVDDLPLT
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCHH
QAVHSQLLKRIGLNPIAVMSGQEALTAVQIADRELHPFGIAFIDLHMPDLNGLEVIAKIR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
ALPLRYQPHCVLVTAAGIQSIVDEAKSVGYAAVLQKPASLAGLREIVSQLLTTQGSQFQY
HCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
LKEKKRPCEALLREHKAGCRILLVEDEPINQMIARELLEDASLVVHVADNGQQAVEMALS
HHHHCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHC
TPSPYDLILMDVQMPVLDGIAAAHCIREKRPDLHTPIIALTANAFSDDRRKCLAAGMNDF
CCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCHHHH
VSKPVDPDVLFEIIWKWLSK
HCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]