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The map label for this gene is phaZ1 [H]

Identifier: 71907686

GI number: 71907686

Start: 2216451

End: 2217482

Strand: Reverse

Name: phaZ1 [H]

Synonym: Daro_2059

Alternate gene names: 71907686

Gene position: 2217482-2216451 (Counterclockwise)

Preceding gene: 71907689

Following gene: 71907684

Centisome position: 49.27

GC content: 63.18

Gene sequence:

>1032_bases
ATGGCAAGGCGAATACGAAAAACGGTCTGGAGCCGATCATTCCAGAGTGCACTGAAGGCAATGACGCGAACGGCAATGCG
TGTCGGCACCAAAGCAATCAAGCAAAGTTTGCGGGCCGCGCCGCTGACGAGCCAACGCAAACCCGTCAAAAAGGCGACGG
CGCACTCGGCCAACTGGACGAAGGGGATCGCCATCGGCGCGGCGGGGCCTCGTGGCTATCGACTCTATAAGCCATCCGGC
ATGCGGCGCAATGAGACGCGGCCCTTGCTCGTCATGCTGCACGGCTGCGGGCAGGATGCCGAAGCGCTGGCTGCCAGTAG
CCGGATGAACACGATTGCAGCCCGTGAGCGATTTTTTGTGCTCTACCCCGAGCAGGATCGCCTGTCGAACGTGCAAGGTT
GCTGGAACTGGTACGACACCCGGACAGGCAGGGCGCAGGCCGAAGCCAATTCGATCAGTGCGGCCATCGAGCAGATTTGC
CTGTCGCAAGCGGTAGACCGCAGTAAAGTTGCCCTGGCCGGAATTTCAGCCGGGGCGGGGATGGCGGTGTTGCTGGCGAC
GCATCATCCGGAACGTTTTCGGGCCATTGCCATGCATTCGGGAATCGCACCGGGGGTTGCGCATTCGTCGGCCAGCGCCA
TCAAGGCCATGTTCGGGCAAAGCGTGACAAGCTCGCCGCTCCCGGCCATTCCGCCCGACGTGAGGCTGCCGGCCTTATTG
GTCATCCACGGTGCAGCCGACCACGTGGTGGCGCCGGGGAATGGTGCCGAAGCGGCCATGCGGTGGGGGGAACGGGTTGG
CGCCAAGACGAGCAAGCCGCGCCTGGTGCAGCGCGGTGCGCGTTATGCGGCGACCATTACCGATTATCGAAAAAGCGGCC
GCTTGGTGGCGACGCTCTGTGCAGTCGACCGGCTTGGGCACGCCTGGAGCGGTGGCGCCGCAGGGCATTCTTACAGCGAT
CCCAAAGGCCCGGACGCATCTCGAATGATCTGGTCTTTCGTCGCCAGGCAATTTGCCCGTTCGGCAGACTAG

Upstream 100 bases:

>100_bases
ATAGCGAACCGCTCTGGCCAATGTTCGAGAGCAGACAGAGGCCGCTGCGGGTATGGCTTGATAATCGGGCCACTGACGAC
ACCGCTTGGAGGCTGCTCAA

Downstream 100 bases:

>100_bases
CAAGCGGATTGGGTGCAGTCGGCCGGTTCTCCATCAAATTGTTTTAGCGTTGAAGTGCTCAGTATTCCCCCTGGCATCCG
GGGCATTCAGCAGTGACCTT

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MARRIRKTVWSRSFQSALKAMTRTAMRVGTKAIKQSLRAAPLTSQRKPVKKATAHSANWTKGIAIGAAGPRGYRLYKPSG
MRRNETRPLLVMLHGCGQDAEALAASSRMNTIAARERFFVLYPEQDRLSNVQGCWNWYDTRTGRAQAEANSISAAIEQIC
LSQAVDRSKVALAGISAGAGMAVLLATHHPERFRAIAMHSGIAPGVAHSSASAIKAMFGQSVTSSPLPAIPPDVRLPALL
VIHGAADHVVAPGNGAEAAMRWGERVGAKTSKPRLVQRGARYAATITDYRKSGRLVATLCAVDRLGHAWSGGAAGHSYSD
PKGPDASRMIWSFVARQFARSAD

Sequences:

>Translated_343_residues
MARRIRKTVWSRSFQSALKAMTRTAMRVGTKAIKQSLRAAPLTSQRKPVKKATAHSANWTKGIAIGAAGPRGYRLYKPSG
MRRNETRPLLVMLHGCGQDAEALAASSRMNTIAARERFFVLYPEQDRLSNVQGCWNWYDTRTGRAQAEANSISAAIEQIC
LSQAVDRSKVALAGISAGAGMAVLLATHHPERFRAIAMHSGIAPGVAHSSASAIKAMFGQSVTSSPLPAIPPDVRLPALL
VIHGAADHVVAPGNGAEAAMRWGERVGAKTSKPRLVQRGARYAATITDYRKSGRLVATLCAVDRLGHAWSGGAAGHSYSD
PKGPDASRMIWSFVARQFARSAD
>Mature_342_residues
ARRIRKTVWSRSFQSALKAMTRTAMRVGTKAIKQSLRAAPLTSQRKPVKKATAHSANWTKGIAIGAAGPRGYRLYKPSGM
RRNETRPLLVMLHGCGQDAEALAASSRMNTIAARERFFVLYPEQDRLSNVQGCWNWYDTRTGRAQAEANSISAAIEQICL
SQAVDRSKVALAGISAGAGMAVLLATHHPERFRAIAMHSGIAPGVAHSSASAIKAMFGQSVTSSPLPAIPPDVRLPALLV
IHGAADHVVAPGNGAEAAMRWGERVGAKTSKPRLVQRGARYAATITDYRKSGRLVATLCAVDRLGHAWSGGAAGHSYSDP
KGPDASRMIWSFVARQFARSAD

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36775; Mature: 36643

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRIRKTVWSRSFQSALKAMTRTAMRVGTKAIKQSLRAAPLTSQRKPVKKATAHSANWT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCC
KGIAIGAAGPRGYRLYKPSGMRRNETRPLLVMLHGCGQDAEALAASSRMNTIAARERFFV
CCEEEECCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEE
LYPEQDRLSNVQGCWNWYDTRTGRAQAEANSISAAIEQICLSQAVDRSKVALAGISAGAG
EECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCC
MAVLLATHHPERFRAIAMHSGIAPGVAHSSASAIKAMFGQSVTSSPLPAIPPDVRLPALL
EEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
VIHGAADHVVAPGNGAEAAMRWGERVGAKTSKPRLVQRGARYAATITDYRKSGRLVATLC
EEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHH
AVDRLGHAWSGGAAGHSYSDPKGPDASRMIWSFVARQFARSAD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARRIRKTVWSRSFQSALKAMTRTAMRVGTKAIKQSLRAAPLTSQRKPVKKATAHSANWT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCC
KGIAIGAAGPRGYRLYKPSGMRRNETRPLLVMLHGCGQDAEALAASSRMNTIAARERFFV
CCEEEECCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEE
LYPEQDRLSNVQGCWNWYDTRTGRAQAEANSISAAIEQICLSQAVDRSKVALAGISAGAG
EECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCC
MAVLLATHHPERFRAIAMHSGIAPGVAHSSASAIKAMFGQSVTSSPLPAIPPDVRLPALL
EEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
VIHGAADHVVAPGNGAEAAMRWGERVGAKTSKPRLVQRGARYAATITDYRKSGRLVATLC
EEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHH
AVDRLGHAWSGGAAGHSYSDPKGPDASRMIWSFVARQFARSAD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]