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The map label for this gene is moaA [H]

Identifier: 71907628

GI number: 71907628

Start: 2152403

End: 2153476

Strand: Reverse

Name: moaA [H]

Synonym: Daro_1999

Alternate gene names: 71907628

Gene position: 2153476-2152403 (Counterclockwise)

Preceding gene: 71907629

Following gene: 71907624

Centisome position: 47.84

GC content: 55.77

Gene sequence:

>1074_bases
ATGCAAAACGAAACCCTCAGGCCGGAATCAACCCCCGCTGGATTTGCCGATTTCACACCGATCATCTCGGCAGCGAGCGG
CGGGCACAGTGAAGGAACGCTACTCGACAAATACGGGCGGCGGATTACTTATGTTCGCCTGTCAATAACCGATCGCTGTG
ACTTTCGTTGCACCTATTGCATGGCGGAGGAAATGACCTTCCTTCCTCGCAAGGAAGTCATGAGCCTGGAAGAATGCCTG
CGCGTTGCGTCGGTCTTTGTCGGACTGGGCGTGACCAAACTGCGGATTACCGGCGGCGAACCCCTGGTTCGCAAAGGGGC
CATGTGGCTGATCGAACGACTGGGCGCCCTGCCCGGTCTCGAAAACCTTGTATTAACCACCAATGGGTCGCAACTGGACA
AGTTTGCAGCCCCATTGCACGCCGCAGGTGTAAAGCGCATCAACATCAGTCTGGACACCCTGAAAGCTGATCGTTTCCGG
GCAATCACCCGCATCGGCGACTTGGCCAAGGTGCTGCGTGGCATCGAGGCAGCCCGTGCCACCGGCTTCCGCAGGACCAA
GCTAAATGCCGTCATGATGCGCGGAACCAACGACGACGAATTCGTTGACCTGGTTCGTTTCGCCATCGATCAGGAGCTGG
ATATTTCCTTCATCGAGGAAATGCCGCTTGGTGAAATCTTTGGACGAAATAGCACCTTCATCTCATCTGAAGAAACCCGG
GAAATGCTCGGACGTCATTTCGACTTGATTCCCTCTGCTGAAAATTCCGGGGGACCGGCTCGTTATTGGCGAATTCCGGG
CAGCGAGTCGCGCGTTGGCTTCATTTCGCCGCATAGCCACAACTTCTGCGACACCTGCAACCGGGTACGGATTACTGCCA
AAGGCGAGCTCTATCCCTGCCTGGGCCAAAACGATGCCGCCAATTTGCTACCGGCGCTGCGCGCCGGAAATGACAATGCC
GATGTACGCCAGGCCATCGTAAACAGCATGGGTATCAAACCGTTTGGCCATGATTTCACCCAACAAATGGATACACCACA
GGTCGTTCGATTTATGTCGATGACAGGCGGCTGA

Upstream 100 bases:

>100_bases
TTTTTTGATCTCTCTACTTGTTCAGTAGATGTTTGACGCTGATAATCGCCGTCCAGACTTCAAGCCCGCATCTTGCGGCT
CGCCAACGAAATTGTCCGCC

Downstream 100 bases:

>100_bases
TTTCCCCTGGCGCTCGGTAGCACCTTGGGCAATCAGCAGCCCGCATCCAATTCCTCACCAGAGAAACGTTAGCCGTACCC
GGACTCAGGGATAGACCCTG

Product: radical SAM:molybdenum cofactor synthesis C-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQNETLRPESTPAGFADFTPIISAASGGHSEGTLLDKYGRRITYVRLSITDRCDFRCTYCMAEEMTFLPRKEVMSLEECL
RVASVFVGLGVTKLRITGGEPLVRKGAMWLIERLGALPGLENLVLTTNGSQLDKFAAPLHAAGVKRINISLDTLKADRFR
AITRIGDLAKVLRGIEAARATGFRRTKLNAVMMRGTNDDEFVDLVRFAIDQELDISFIEEMPLGEIFGRNSTFISSEETR
EMLGRHFDLIPSAENSGGPARYWRIPGSESRVGFISPHSHNFCDTCNRVRITAKGELYPCLGQNDAANLLPALRAGNDNA
DVRQAIVNSMGIKPFGHDFTQQMDTPQVVRFMSMTGG

Sequences:

>Translated_357_residues
MQNETLRPESTPAGFADFTPIISAASGGHSEGTLLDKYGRRITYVRLSITDRCDFRCTYCMAEEMTFLPRKEVMSLEECL
RVASVFVGLGVTKLRITGGEPLVRKGAMWLIERLGALPGLENLVLTTNGSQLDKFAAPLHAAGVKRINISLDTLKADRFR
AITRIGDLAKVLRGIEAARATGFRRTKLNAVMMRGTNDDEFVDLVRFAIDQELDISFIEEMPLGEIFGRNSTFISSEETR
EMLGRHFDLIPSAENSGGPARYWRIPGSESRVGFISPHSHNFCDTCNRVRITAKGELYPCLGQNDAANLLPALRAGNDNA
DVRQAIVNSMGIKPFGHDFTQQMDTPQVVRFMSMTGG
>Mature_357_residues
MQNETLRPESTPAGFADFTPIISAASGGHSEGTLLDKYGRRITYVRLSITDRCDFRCTYCMAEEMTFLPRKEVMSLEECL
RVASVFVGLGVTKLRITGGEPLVRKGAMWLIERLGALPGLENLVLTTNGSQLDKFAAPLHAAGVKRINISLDTLKADRFR
AITRIGDLAKVLRGIEAARATGFRRTKLNAVMMRGTNDDEFVDLVRFAIDQELDISFIEEMPLGEIFGRNSTFISSEETR
EMLGRHFDLIPSAENSGGPARYWRIPGSESRVGFISPHSHNFCDTCNRVRITAKGELYPCLGQNDAANLLPALRAGNDNA
DVRQAIVNSMGIKPFGHDFTQQMDTPQVVRFMSMTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=327, Percent_Identity=36.697247706422, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI22027536, Length=327, Percent_Identity=36.697247706422, Blast_Score=209, Evalue=3e-54,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=32.5227963525836, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566331, Length=317, Percent_Identity=36.5930599369085, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32566329, Length=340, Percent_Identity=36.1764705882353, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17561210, Length=310, Percent_Identity=37.0967741935484, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI28574490, Length=302, Percent_Identity=39.7350993377483, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28574488, Length=302, Percent_Identity=39.7350993377483, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39473; Mature: 39473

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNETLRPESTPAGFADFTPIISAASGGHSEGTLLDKYGRRITYVRLSITDRCDFRCTYC
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEEEECCCCCCEEEEE
MAEEMTFLPRKEVMSLEECLRVASVFVGLGVTKLRITGGEPLVRKGAMWLIERLGALPGL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
ENLVLTTNGSQLDKFAAPLHAAGVKRINISLDTLKADRFRAITRIGDLAKVLRGIEAARA
CCEEEECCCCHHHHHHCCHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGFRRTKLNAVMMRGTNDDEFVDLVRFAIDQELDISFIEEMPLGEIFGRNSTFISSEETR
CCCHHEEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCEECCHHHH
EMLGRHFDLIPSAENSGGPARYWRIPGSESRVGFISPHSHNFCDTCNRVRITAKGELYPC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHCCCEEEEEECCCEEEC
LGQNDAANLLPALRAGNDNADVRQAIVNSMGIKPFGHDFTQQMDTPQVVRFMSMTGG
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQNETLRPESTPAGFADFTPIISAASGGHSEGTLLDKYGRRITYVRLSITDRCDFRCTYC
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCEEEEEEEEECCCCCCEEEEE
MAEEMTFLPRKEVMSLEECLRVASVFVGLGVTKLRITGGEPLVRKGAMWLIERLGALPGL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
ENLVLTTNGSQLDKFAAPLHAAGVKRINISLDTLKADRFRAITRIGDLAKVLRGIEAARA
CCEEEECCCCHHHHHHCCHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGFRRTKLNAVMMRGTNDDEFVDLVRFAIDQELDISFIEEMPLGEIFGRNSTFISSEETR
CCCHHEEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCEECCHHHH
EMLGRHFDLIPSAENSGGPARYWRIPGSESRVGFISPHSHNFCDTCNRVRITAKGELYPC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHCCCEEEEEECCCEEEC
LGQNDAANLLPALRAGNDNADVRQAIVNSMGIKPFGHDFTQQMDTPQVVRFMSMTGG
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA