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The map label for this gene is pleD [H]

Identifier: 71907401

GI number: 71907401

Start: 1906192

End: 1907541

Strand: Reverse

Name: pleD [H]

Synonym: Daro_1771

Alternate gene names: 71907401

Gene position: 1907541-1906192 (Counterclockwise)

Preceding gene: 71907402

Following gene: 71907399

Centisome position: 42.38

GC content: 57.85

Gene sequence:

>1350_bases
ATGTCCCCACGCAAACCGCGCATCCTGGCCATCGACGATATGCCGGGAAATCTATATACGCTAGGGACCGCCCTGGCTAA
CGACTTCGACCTGGAAATCGCCACCTCGGGCAGTCAGGGACTGACCATGGCAAAACATTCGCCCCCCGATCTGGTCCTGC
TCGATATCATGATGCCGGAAATGGATGGCCTTGAAACCTGTCGCCGTTTCCGCAGTGACCCTCGCCTGCAGGCCGTTCCC
ATCATCTTCCTGACCGCGCTCACCGACGTCGACTCGGAGGTGACAGGCCTCGATCTTGGCGTGGCAGACTACATCACCAA
GCCATTCCACCTCGATATCGTCAAACTACGTATCCGAAACACCCTCAAGTTAGCCAGCCTAAACCGGGAATTGCGGGCCA
GCGAAGAACGCATGCGCTTCGTGCTCGACGCCACCGGCGATGGCATCTGGGACTGGGAGATTGAATTCAATCGCGTCAGT
CACAACGAGGCCTGGTGCCGCATGATGGGTTTGGATACGAGTTTTCTCAGCCACCCGCTCGCGGATTACGTCGAGCGCAT
TCACCCCGAAGACCTCGCTGCGGTCGAGCGGGCGCTGGATATCAGCACCCATGAAAACACGCTCTTCACCGCTGAATACC
GCATCCGGCATAGTGATGGGCACTATTTGTGGATATCGGATACCGGCCAGGTCGTTGACCGGGACATCGACGGCAAGGCG
AGCCGCATGGTTGGCTGCGTCAAGAATATCGATGAGCGGAAACGCAGCGAAGCCGATATTCACAGACTGGCTTTTTTCGA
CGCACTGACCGAACTGCCCAATCGCCGGCTATTGATGGACCGCCTGCAACAGGCCATTTTCCACAACACCCGGAATAACA
GCCTGGGTGCCCTGTTATTCCTGGACATGGACCACTTCAAGCAACTGAACGACACCCACGGACATGCCATGGGCGATGCC
ATGCTCATTCAGGTTGCTTCACGGCTTAGACGCAACGTGCGCGAACAGGACACAGTTTCGCGCCTGGGAGGGGACGAATT
CGTTGTCCTGCTCGAAAGCCTGGCCCCTGACCCGGTTAGCGCCGGCAATGCGGCAACACTCATTGGCGAGAAGTTGCTGG
GCGCCTTGAATAGTCCTTACCAGTTGGGCTCCATCAAGCACCACAGTAGCCCCAGTATCGGCCTCACCCTGTTTGGTGGT
GACAACGAACAACTCGATGAGATCCTGAAGCGGGCAGACCATGCCATGTACGAAGCCAAGGGGGCCGGACGCAACACCTT
GCGCACACTGATCGATACCGAGCAACTACGTGCCACCCAGCCCAATCGGGGCAAGCCAACATCCGCTTGA

Upstream 100 bases:

>100_bases
CGAAATCGACGAAATCGCAGACTCAGTCAAGACCTTTCAGTTCCAAGCCGCACTGGGCCGCCTGCGTGCCCTGGTCAATG
GCTAGCTCCCGAGGAATAAC

Downstream 100 bases:

>100_bases
ACGAATCGATCGCAACGAAAAATACGCCAAGCTTGCAATAAAAAAGCCGGTTACCCGGCTTTCTTCATTGATGGCCACCT
TTGGCCGGCGGAGCCCCGGG

Product: PAS:GGDEF

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSPRKPRILAIDDMPGNLYTLGTALANDFDLEIATSGSQGLTMAKHSPPDLVLLDIMMPEMDGLETCRRFRSDPRLQAVP
IIFLTALTDVDSEVTGLDLGVADYITKPFHLDIVKLRIRNTLKLASLNRELRASEERMRFVLDATGDGIWDWEIEFNRVS
HNEAWCRMMGLDTSFLSHPLADYVERIHPEDLAAVERALDISTHENTLFTAEYRIRHSDGHYLWISDTGQVVDRDIDGKA
SRMVGCVKNIDERKRSEADIHRLAFFDALTELPNRRLLMDRLQQAIFHNTRNNSLGALLFLDMDHFKQLNDTHGHAMGDA
MLIQVASRLRRNVREQDTVSRLGGDEFVVLLESLAPDPVSAGNAATLIGEKLLGALNSPYQLGSIKHHSSPSIGLTLFGG
DNEQLDEILKRADHAMYEAKGAGRNTLRTLIDTEQLRATQPNRGKPTSA

Sequences:

>Translated_449_residues
MSPRKPRILAIDDMPGNLYTLGTALANDFDLEIATSGSQGLTMAKHSPPDLVLLDIMMPEMDGLETCRRFRSDPRLQAVP
IIFLTALTDVDSEVTGLDLGVADYITKPFHLDIVKLRIRNTLKLASLNRELRASEERMRFVLDATGDGIWDWEIEFNRVS
HNEAWCRMMGLDTSFLSHPLADYVERIHPEDLAAVERALDISTHENTLFTAEYRIRHSDGHYLWISDTGQVVDRDIDGKA
SRMVGCVKNIDERKRSEADIHRLAFFDALTELPNRRLLMDRLQQAIFHNTRNNSLGALLFLDMDHFKQLNDTHGHAMGDA
MLIQVASRLRRNVREQDTVSRLGGDEFVVLLESLAPDPVSAGNAATLIGEKLLGALNSPYQLGSIKHHSSPSIGLTLFGG
DNEQLDEILKRADHAMYEAKGAGRNTLRTLIDTEQLRATQPNRGKPTSA
>Mature_448_residues
SPRKPRILAIDDMPGNLYTLGTALANDFDLEIATSGSQGLTMAKHSPPDLVLLDIMMPEMDGLETCRRFRSDPRLQAVPI
IFLTALTDVDSEVTGLDLGVADYITKPFHLDIVKLRIRNTLKLASLNRELRASEERMRFVLDATGDGIWDWEIEFNRVSH
NEAWCRMMGLDTSFLSHPLADYVERIHPEDLAAVERALDISTHENTLFTAEYRIRHSDGHYLWISDTGQVVDRDIDGKAS
RMVGCVKNIDERKRSEADIHRLAFFDALTELPNRRLLMDRLQQAIFHNTRNNSLGALLFLDMDHFKQLNDTHGHAMGDAM
LIQVASRLRRNVREQDTVSRLGGDEFVVLLESLAPDPVSAGNAATLIGEKLLGALNSPYQLGSIKHHSSPSIGLTLFGGD
NEQLDEILKRADHAMYEAKGAGRNTLRTLIDTEQLRATQPNRGKPTSA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=32.9787234042553, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=204, Percent_Identity=31.8627450980392, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=34.4262295081967, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=36.8098159509202, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=37.7952755905512, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=33.9506172839506, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788394, Length=143, Percent_Identity=33.5664335664336, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=35.2272727272727, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=31.3186813186813, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787262, Length=215, Percent_Identity=32.5581395348837, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=36.5269461077844, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789149, Length=103, Percent_Identity=36.8932038834951, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081974, Length=102, Percent_Identity=35.2941176470588, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786784, Length=95, Percent_Identity=36.8421052631579, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=111, Percent_Identity=36.036036036036, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=32.2033898305085, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50313; Mature: 50182

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRKPRILAIDDMPGNLYTLGTALANDFDLEIATSGSQGLTMAKHSPPDLVLLDIMMPE
CCCCCCCEEEEECCCCCEEEHHHHHCCCCCEEEEECCCCCCEECCCCCCCEEEEEECCCC
MDGLETCRRFRSDPRLQAVPIIFLTALTDVDSEVTGLDLGVADYITKPFHLDIVKLRIRN
CCHHHHHHHHCCCCCCEEHHHEEEEHHHCCCCCCCCCCCCHHHHHCCCCEEEEEEEEHHH
TLKLASLNRELRASEERMRFVLDATGDGIWDWEIEFNRVSHNEAWCRMMGLDTSFLSHPL
HHHHHHHCHHHHCCHHHHEEEEECCCCCEEEEEEEEEECCCCHHHHHEECCCHHHHHCHH
ADYVERIHPEDLAAVERALDISTHENTLFTAEYRIRHSDGHYLWISDTGQVVDRDIDGKA
HHHHHHHCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEECCCCCEECCCCCCHH
SRMVGCVKNIDERKRSEADIHRLAFFDALTELPNRRLLMDRLQQAIFHNTRNNSLGALLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LDMDHFKQLNDTHGHAMGDAMLIQVASRLRRNVREQDTVSRLGGDEFVVLLESLAPDPVS
EEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCC
AGNAATLIGEKLLGALNSPYQLGSIKHHSSPSIGLTLFGGDNEQLDEILKRADHAMYEAK
CCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
GAGRNTLRTLIDTEQLRATQPNRGKPTSA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SPRKPRILAIDDMPGNLYTLGTALANDFDLEIATSGSQGLTMAKHSPPDLVLLDIMMPE
CCCCCCEEEEECCCCCEEEHHHHHCCCCCEEEEECCCCCCEECCCCCCCEEEEEECCCC
MDGLETCRRFRSDPRLQAVPIIFLTALTDVDSEVTGLDLGVADYITKPFHLDIVKLRIRN
CCHHHHHHHHCCCCCCEEHHHEEEEHHHCCCCCCCCCCCCHHHHHCCCCEEEEEEEEHHH
TLKLASLNRELRASEERMRFVLDATGDGIWDWEIEFNRVSHNEAWCRMMGLDTSFLSHPL
HHHHHHHCHHHHCCHHHHEEEEECCCCCEEEEEEEEEECCCCHHHHHEECCCHHHHHCHH
ADYVERIHPEDLAAVERALDISTHENTLFTAEYRIRHSDGHYLWISDTGQVVDRDIDGKA
HHHHHHHCHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEECCCCCEECCCCCCHH
SRMVGCVKNIDERKRSEADIHRLAFFDALTELPNRRLLMDRLQQAIFHNTRNNSLGALLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LDMDHFKQLNDTHGHAMGDAMLIQVASRLRRNVREQDTVSRLGGDEFVVLLESLAPDPVS
EEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCC
AGNAATLIGEKLLGALNSPYQLGSIKHHSSPSIGLTLFGGDNEQLDEILKRADHAMYEAK
CCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
GAGRNTLRTLIDTEQLRATQPNRGKPTSA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA